US2003040619A1PendingUtilityA1
Process for altering the host range of bacillus thuringiensis toxins, and novel toxins produced thereby
Priority: Dec 12, 1985Filed: Dec 27, 2001Published: Feb 27, 2003
Est. expiryDec 12, 2005(expired)· nominal 20-yr term from priority
C07K 14/325C12R 2001/075C12N 1/205A01N 63/50
53
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Claims
Abstract
The invention concerns an in vitro process for altering the insect host range (spectrum) of pesticidal toxins. The process comprises recombining in vitro the variable region(s) (non-homologous) of two or more genes encoding a pesticidal toxin. Specifically exemplified is the recombining of the variable regions of two genes obtained from well-known strains of Bacillius thuringiensis var. kurstaki. The resulting products are chimeric toxins which are shown to have an expanded and/or amplified insect host range as compared to the parent toxins.
Claims
exact text as granted — not AI-modified1 . A process for altering the host range of Bacillus toxins which comprises recombining in vitro the variable region of two or more Bacillus toxin genes.
2 . A process, according to claim 1 , wherein the Bacillus is a Bacillius thuringiensis.
3 . A process, according to claim 2 , wherein variable regions of Bacillius thuringiensis var. kurstaki HD-1 and Bacillius thuringiensis var. kurstaki HD-73 are recombined in vitro to give genes encoding chimeric toxins having altered host ranges.
4 . DNA, denoted pEW3, encoding a chimeric toxin having pesticidal activity, as follows:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
AAGAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATAGG GGTTATTATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTGGAT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG GATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGGA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1)
and equivalent nucleotide sequences coding for toxin EW3 with the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E R S I R S P H L M D I L
N S I T I Y T D A H R G Y Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L D E I P P Q N N N V
P P R Q G F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N Q L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E.
5 . DNA, denoted pEW4, encoding a chimeric toxin, having pesticidal activity, as follows:
(start HD-1) ATGG ATAACAATCC GAACATCAAT
GAATGCATTC CTTATAATTG TTTAAGTAAC CCTGAAGTAG AAGTATTAGG
600
TGGAGAAAGA ATAGAAACTG GTTACACCCC AATCGATATT TCCTTGTCGC
TAACGCAATT TCTTTTGAGT GAATTTGTTC CCGGTGCTGG ATTTGTGTTA
700
GGACTAGTTG ATATAATATG GGGAATTTTT GGTCCCTCTC AATGGGACGC
ATTTCCTGTA CAAATTGAAC AGTTAATTAA CCAAAGAATA GAAGAATTCG
800
CTAGGAACCA AGCCATTTCT AGATTAGAAG GACTAAGCAA TCTTTATCAA
ATTTACGCAG AATCTTTTAG AGAGTGGGAA GCAGATCCTA CTAATCCAGC
900
ATTAAGAGAA GAGATGCGTA TTCAATTCAA TGACATGAAC AGTGCCCTTA
CAACCGCTAT TCCTCTTTTG GCAGTTCAAA ATTATCAAGT TCCTCTTTTA
1000
TCAGTATATG TTCAAGCTGC AAATTTACAT TTATCAGTTT TGAGAGATGT
TTCAGTGTTT GGACAAAGGT GGGGATTTGA TGCCGCGACT ATCAATAGTC
1100
GTTATAATGA TTTAACTAGG CTTATTGGCA ACTATACAGA TTATGCTGTG
CGCTGGTACA ATACGGGATT AGAGCGTGTA TGGGGACCGG ATTCTAGAGA
1200
TTGGGTAAGG TATAATCAAT TTAGAAGAGA GCTAACACTT ACTGTATTAG
ATATCGTTGC TCTATTCTCA AATTATGATA GTCGAAGGTA TCCAATTCGA
1300
ACAGTTTCCC AATTAACAAG AGAAATTTAT ACGAACCCAG TATTAGAAAA
TTTTGATGGT AGTTTTCGTG GAATGGCTCA GAGAATAGAA CAGAATATTA
1400
GGCAACCACA TCTTATGGAT ATCCTTAATA GTATAACCAT TTATACTGAT
GTGCATAGAG GCTTTAATTA TTGGTCAGGG CATCAAATAA CAGCTTCTCC
1500
TGTAGGGTTT TCAGGACCAG AATTCGCATT CCCTTTATTT GGGAATGCGG
GGAATGCAGC TCCACCCGTA CTTGTCTCAT TAACTGGTTT GGGGPTTTTT
1600
AGAACATTAT CTTCACCTTT ATATAGAAGA ATTATACTTG GTTCAGGCCC
AAATAATCAG GAACTGTTTG TCCTTGATGG AACGGAGTTT TCTTTTGCCT
1700
CCCTAACGAC CAACTTGCCT TCCACTATAT ATAGACAAAG GGGTACAGTC
GATTCACTAG ATGTAATACC GCCACAGGAT AATAGTGTAC CACCTCGTGC
1800
GGGATTTAGC CATCGATTGA GTCATGTTAC AATGCTGAGC CAAGCAGCTG
GAGCAGTTTA CACCTTGAGA GCTCAACGT (stop HD-1)
(start HD-73) CCT ATGTTCTCTT
GGATACATCG TAGTGCTGAA TTTAATAATA TAATTGCATC GGATAGTATT
1800
ACTCAAATCC CTGCAGTGAA GGGAAACTTT CTTTTTAATG GTTCTGTAAT
TTCAGGACCA GGATTTACTG GTGGGGACTT AGTTAGATTA AATAGTAGTG
1900
GAAATAACAT TCAGAATAGA GGGTATATTG AAGTTCCAAT TCACTTCCCA
TCGACATCTA CCAGATATCG AGTTCGTGTA CGGTATGCTT CTGTAACCCC
2000
GATTCACCTC AACGTTAATT GGGGTAATTC ATCCATTTTT TCCAATACAG
TACCAGCTAC AGCTACGTCA TTAGATAATC TACAATCAAG TGATTTTGGT
2100
TATTTTGAAA GTGCCAATGC TTTTACATCT TCATTAGGTA ATATAGTAGG
TGTTAGAAAT TTTAGTGGGA CTGCAGGAGT GATAATAGAC AGATTTGAAT
2200
TTATTCCAGT TACTGCAACA CTCGAGGCTG AATATAATCT GGAAAGAGCG
CAGAAGGCGG TGAATGCGCT GTTTACGTCT ACAAACCAAC TAGGGCTAAA
2300
AACAAATGTA ACGGATTATC ATATTGATCA AGTGTCCAAT TTAGTTACGT
ATTTATCGGA TGAATTTTGT CTGGATGAAA AGCGAGAATT GTCCGAGAAA
2400
GTCAAACATG CGAAGCGACT CAGTGATGAA CGCAATTTAC TCCAAGATTC
AAATTTCAAA GACATTAATA GGCAACCAGA ACGTGGGTGG GGCGGAAGTA
2500
CAGGGATTAC CATCCAAGGA GGGGATGACG TATTTAAAGA AAATTACGTC
ACACTATCAG GTACCTTTGA TGAGTGCTAT CCAACATATT TGTATCAAAA
2600
AATCGATGAA TCAAAATTAA AAGCCTTTAC CCGTTATCAA TTAAGAGGGT
ATATCGAAGA TAGTCAAGAC TTAGAAATCT ATTTAATTCG CTACAATGCA
2700
AAACATGAAA CAGTAAATGT GCCAGGTACG GGTTCCTTAT GGCCGCTTTC
AGCCCAAAGT CCAATCGGAA AGTGTGGAGA GCCGAATCGA TGCGCGCCAC
2800
ACCTTGAATG GAATCCTGAC TTAGATTGTT CGTGTAGGGA TGGAGAAAAG
TGTGCCCATC ATTCGCATCA TTTCTCCTTA GACATTGATG TAGGATGTAC
2900
AGACTTAAAT GAGGACCTAG GTGTATGGGT GATCTTTAAG ATTAAGACGC
AAGATGGGCA CGCAAGACTA GGGAATCTAG AGTTTCTCGA AGAGAAACCA
3000
TTAGTAGGAG AAGCGCTAGC TCGTGTGAAA AGAGCGGAGA AAAAATGGAG
AGACAAACGT GAAAAATTGG AATGGGAAAC AAATATCGTT TATAAAGAGG
3100
CAAAAGAATC TGTAGATGCT TTATTTGTAA ACTCTCAATA TGATCAATTA
CAAGCGGATA CGAATATTGC CATGATTCAT GCGGCAGATA AACGTGTTCA
3200
TAGCATTCGA GAAGCTTATC TGCCTGAGCT GTCTGTGATT CCGGGTGTCA
ATGCGGCTAT TTTTGAAGAA TTAGAAGGGC GTATTTTCAC TGCATTCTCC
3300
CTATATGATG CGAGAAATGT CATTAAAAAT GGTGATTTTA ATAATGGCTT
ATCCTGCTGG AACGTGAAAG GGCATGTAGA TGTAGAAGAA CAAAACAACC
3400
AACGTTCGGT CCTTGTTGTT CCGGAATGGG AAGCAGAAGT GTCACAAGAA
GTTCGTGTCT GTCCGGGTCG TGGCTATATC CTTCGTGTCA CAGCGTACAA
3500
GGAGGGATAT GGAGAAGGTT GCGTAACCAT TCATGAGATC GAGAACAATA
CAGACGAACT GAAGTTTAGC AACTGCGTAG AAGAGGAAAT CTATCCAAAT
3600
AACACGGTAA CGTGTAATGA TTATACTGTA AATCAAGAAG AATACGGAGG
TGCGTACACT TCTCGTAATC GAGGATATAA CGAAGCTCCT TCCGTACCAG
3700
CTGATTATGC GTCAGTCTAT GAAGAAAAAT CGTATACAGA TGGACGAAGA
GAGAATCCTT GTGAATTTAA CAGAGGGTAT AGGGATTACA CGCCACTACC
3800
AGTTGGTTAT GTGACAAAAG AATTAGAATA CTTCCCAGAA ACCGATAAGG
TATGGATTGA GATTGGAGAA ACGGAAGGAA CATTTATCGT GGACAGCGTG
3900
GAATTACTCC TTATGGAGGA A (end HD-73)
and equivalent nucleotide sequences coding for toxin EW4 with the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F P V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L L A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F S N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G M A Q R I E Q N I R Q P H L M D I L
N S I T I Y T D V H R G F N Y W S G H Q I T A S P V G F S G
P E F A F P L F G N A G N A A P P V L V S L T G L G I F R T
L S S P L Y R R I I L G S G P N N Q E L F V L D G T E F S F
A S L T T N L P S T I Y R Q R G T V D S L D V I P P Q D N S
V P P R A G F S H R L S H V T M L S Q A A G A V Y T L R A Q
R P M F S W I H R S A E F N N I I A S D S I T Q I P A V K G
N F L F N G S V I S G P G F T G G D L V R L N S S G N N I Q
N R G Y I E V P I H F P S T S T R Y R V R V R Y A S V T P I
H L N V N W G N S S I F S N T V P A T A T S L D N L Q S S D
F G Y F E S A N A F T S S L G N I V G V R N F S G T A G V I
I D R F E F I P V T A T L E A E Y N L E R A Q K A V N A L F
T S T N Q L G L K T N V T D Y H I D Q V S N L V T Y L S D E
F C L D E K R E L S E K V K H A K R L S D E R N L L Q D S N
F K D I N R Q P E R G W G G S T G I T I Q G G D D V F K E N
Y V T L S G T F D E C Y P T Y L Y Q K I D E S K L K A F T R
Y Q L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P
G T G S L W P L S A Q S P I G K C G E P N R C A P H L E W N
P D L D C S C R D G E K C A H H S H H F S L D I D V G C T D
L N E D L G V W V I F K I K T Q D G H A R L G N L E F L E E
K P L V G E A L A R V K R A E K K W R D K R E K L E W E T N
I V Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M
I H A A D K R V H S I R E A Y L P E L S V I P G V N A A I F
E E L E G R I F T A F S L Y D A R N V I K N G D F N N G L S
C W N V K G H V D V E E Q N N Q R S V L V V P E W E A E V S
Q E V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H
E I E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y
T V N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S
V Y E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V
G Y V T K E L E Y F P E T D K V W I E I G E T E G T F I V D
S V E L L L M E E.
6 . DNA, denoted pACB-l, encoding a chimeric toxin, having pesticidal activity, as follows:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
AAGAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATAGG GGTTATTATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTG A AT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG A ATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1)
and equivalent; nucleotide sequences coding for toxin ACB-1 with the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E R S I R S P H L M D I L
N S I T I Y T D A H R G Y Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L N E I P P Q N N N V
P P R Q E F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E.
7 . DNA, denoted pSYW1, encoding a chimeric toxin, having pesticidal activity, as follows:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
A G GAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATA AA GG GG A A TATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTGGAT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG GATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1)
and equivalent nucleotide sequences coding for toxin SYW1 with the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E G S I R S P H L M D I L
N S I T I Y T D A H K G E Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L D E I P P Q N N N V
P P R Q G F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A P L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E.
8 . A chimeric toxin, EW3, having pesticidal activity, having the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E R S I R S P H L M D I L
N S I T I Y T D A H R G Y Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L D E I P P Q N N N V
P P R Q G F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E
and muteins thereof which do not alter the protein secondary structure.
9 . A chimeric toxin, EW4, having pesticidal activity, having the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F P V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L L A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F S N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G M A Q R I E Q N I R Q P H L M D I L
N S I T I Y T D V H R G F N Y W S G H Q I T A S P V G F S G
P E F A F P L F G N A G N A A P P V L V S L T G L G I F R T
L S S P L Y R R I I L G S G P N N Q E L F V L D G T E F S F
A S L T T N L P S T I Y R Q R G T V D S L D V I P P Q D N S
V P P R A G F S H R L S H V T M L S Q A A G A V Y T L R A Q
R P M F S W I H R S A E F N N I I A S D S I T Q I P A V K G
N F L F N G S V I S G P G F T G G D L V R L N S S G N N I Q
N R G Y I E V P I H F P S T S T R Y R V R V R Y A S V T P I
H L N V N W G N S S I F S N T V P A T A T S L D N L Q S S D
F G Y F E S A N A F T S S L G N I V G V R N F S G T A G V I
I D R F E F I P V T A T L E A E Y N L E R A Q K A V N A L F
T S T N Q L G L K T N V T D Y H I D Q V S N L V T Y L S D E
F C L D E K R E L S E K V K H A K R L S D E R N L L Q D S N
F K D I N R Q P E R G W G G S T G I T I Q G G D D V F K E N
Y V T L S G T F D E C Y P T Y L Y Q K I D E S K L K A F T R
Y Q L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P
G T G S L W P L S A Q S P I G K C G E P N R C A P H L E W N
P D L D C S C R D G E K C A H H S H H F S L D I D V G C T D
L N E D L G V W V I F K I K T Q D G H A R L G N L E F L E E
K P L V G E A L A R V K R A E K K W R D K R E K L E W E T N
I V Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M
I H A A D K R V H S I R E A Y L P E L S V I P G V N A A I F
E E L E G R I F T A F S L Y D A R N V I K N G D F N N G L S
C W N V K G H V D V E E Q N N Q R S V L V V P E W E A E V S
Q E V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H
E I E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y
T V N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S
V Y E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V
G Y V T K E L E Y F P E T D K V W I E I G E T E G T F I V D
S V E L L L M E E
and muteins thereof which do not alter the protein secondary structure.
10 . A chimeric toxin, ACB-1, having pesticidal activity, having the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E R S I R S P H L M D I L
N S I T I Y T D A H R G Y Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L N E I P P Q N N N V
P P R Q E F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E
and muteins thereof which do not alter the protein secondary structure.
11 . A chimeric toxin, SYW1, having pesticidal activity, having the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E G S I R S P H L M D I L
N S I T I Y T D A H K G E Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L D E I P P Q N N N V
P P R Q G F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E
and muteins thereof which do not alter the protein secondary structure.
12 . A pesticidal composition comprising pesticide-containing substantially intact cells having prolonged pesticidal activity when applied to the environment of a target pest, wherein said pesticide is a chimeric toxin, is intracellular and is produced as a result of expression of a heterologous gene encoding said chimeric toxin in said cell.
13 . A pesticidal composition according to claim 12 , wherein said cells are killed under protease deactivating or cell wall strengthening conditions, while retaining pesticidal activity.
14 . A pesticidal composition, according to claim 12 , wherein said cells are prokaryotes selected from the group consisting of Enterobacteriaceae, Bacillaceae, Rhizobiaceae, Spirillaceae, Lactobacillaceae, Pseudomonadaceae, Azotobacteraceae, and Nitrobacteraceae; or lower eukaryotes selected from the group consisting of Phycomycetes, Ascomycetes, and Basidiomycetes.
15 . A pesticidal composition, according to claim 14 , wherein said prokaryote is a Bacillus specie selected from a pesticide-Producing strain of Bacillus thuringiensis, consisting of B. thuringiensis M-7, B. thuringiensis var. kurstaki, B. thuringiensis var. finitimus, B. thuringiensis var. alesti, B. thuringiensis var. sotto, B. thuringiensis var. dendrolimus, B. thuringiensis var. kenyae B. thuringiensis var. galleriae, B. thuringiensis var. canadensis, B. thuringiensis var. entomocidus, B. thuringiensis var. subtoxicus, B. thuringiensis var. aizawai, B. thuringiensis var. morrisoni, B. thuringiensis var. ostriniae, B. thuringiensis var. tolworthi, B. thuringiensis var. darmstadiensis, B. thuringiensis var. toumanoffi, B. thuringiensis var. kyushuensis, B. thuringiensis var. thompsoni, B. thuringiensis var. pakistani, B. thuringiensis var. israelensis, B. thuringiensis var. indiana, B. thuringiensis var. dakota, B. thuringiensis var. tohokuensis, B. thuringiensis var. kumanotoensis, B. thuringiensis var. tochigiensis, B. thuringiensis var. colmeri, B. thuringiensis var. wuhanensis, B. thuringiensis var. tenebrionis, B. thuringiensis var. thuringiensis, and other Bacillus species selected from B. cereus, B. moritai, B. popilliae, B. lentimorbus, and B. sphaericus.
16 . A method of protecting plants against pests which comprises applying to said plants an effective amount of a pesticidal composition comprising pesticide-containing substantially intact unicellular microorganisms, wherein said pesticide is a chimeric toxin, is intracellular, and is produced as a result of expression of a heterologous gene encoding said chimeric toxin in said microorganism, and said microorganism is treated under conditions which prolong the pesticidal activity when said composition is applied to the environment of a target pest.
17 . A method according to claim 16 , wherein said microorganisms are prokaryotes selected from the group consisting of Enterobacteriaceae, Bacillaceae, Rhizobiaceae, Spirillaceae, Lactobacillaceae, Pseudomonadaceae, Azotobacteraceae, and Nitrobacteraceae; or lower eukaryotes, selected from the group consisting of Phycomycetes, Ascomycetes, and Basidiomycetes.
18 . A method according to claim 16 , wherein said unicellular microorganisms are killed under protease deactivating or cell wall strengthening conditions, while retaining pesticidal activity.
19 . Substantially intact unicellular microorganism cells containing an intracellular chimeric toxin, which toxin is a result of expression of a heterologous gene encoding said chimeric toxin, wherein said cells are killed under protease deactivating or cell wall strengthening conditions, while retaining pesticidal activity when said cell is applied to the environment of a target pest.
20 . Cells according to claim 19 , wherein said microorganism is a Pseudomonad and said toxin is derived from a B. thuringiensis.
21 . A pesticidal composition, according to claim 12 , wherein said gene, denoted pEW3, encoding a chimeric toxin, is as follows:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
AAGAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATAGG GGTTATTATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTGGAT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG GATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1)
and equivalent nucleotide sequences coding for toxin EW3 with the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E R S I R S P H L M D I L
N S I T I Y T D A H R G Y Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L D E I P P Q N N N V
P P R Q G F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E.
22 . A pesticidal composition, according to claim 12 , wherein said gene, denoted pEW4, encoding a chimeric toxin, is as follows:
(start HD-1) ATGG ATAACAATCC GAACATCAAT
GAATGCATTC CTTATAATTG TTTAAGTAAC CCTGAAGTAG AAGTATTAGG
600
TGGAGAAAGA ATAGAAACTG GTTACACCCC AATCGATATT TCCTTGTCGC
TAACGCAATT TCTTTTGAGT GAATTTGTTC CCGGTGCTGG ATTTGTGTTA
700
GGACTAGTTG ATATAATATG GGGAATTTTT GGTCCCTCTC AATGGGACGC
ATTTCCTGTA CAAATTGAAC AGTTAATTAA CCAAAGAATA GAAGAATTCG
800
CTAGGAACCA AGCCATTTCT AGATTAGAAG GACTAAGCAA TCTTTATCAA
ATTTACGCAG AATCTTTTAG AGAGTGGGAA GCAGATCCTA CTAATCCAGC
900
ATTAAGAGAA GAGATGCGTA TTCAATTCAA TGACATGAAC AGTGCCCTTA
CAACCGCTAT TCCTCTTTTG GCAGTTCAAA ATTATCAAGT TCCTCTTTTA
1000
TCAGTATATG TTCAAGCTGC AAATTTACAT TTATCAGTTT TGAGAGATGT
TTCAGTGTTT GGACAAAGGT GGGGATTTGA TGCCGCGACT ATCAATAGTC
1100
GTTATAATGA TTTAACTAGG CTTATTGGCA ACTATACAGA TTATGCTGTG
CGCTGGTACA ATACGGGATT AGAGCGTGTA TGGGGACCGG ATTCTAGAGA
1200
TTGGGTAAGG TATAATCAAT TTAGAAGAGA GCTAACACTT ACTGTATTAG
ATATCGTTGC TCTATTCTCA AATTATGATA GTCGAAGGTA TCCAATTCGA
1300
ACAGTTTCCC AATTAACAAG AGAAATTTAT ACGAACCCAG TATTAGAAAA
TTTTGATGGT AGTTTTCGTG GAATGGCTCA GAGAATAGAA CAGAATATTA
1400
GGCAACCACA TCTTATGGAT ATCCTTAATA GTATAACCAT TTATACTGAT
GTGCATAGAG GCTTTAATTA TTGGTCAGGG CATCAAATAA CAGCTTCTCC
1500
TGTAGGGTTT TCAGGACCAG AATTCGCATT CCCTTTATTT GGGAATGCGG
GGAATGCAGC TCCACCCGTA CTTGTCTCAT TAACTGGTTT GGGGATTTTT
1600
AGAACATTAT CTTCACCTTT ATATAGAAGA ATTATACTTG GTTCAGGCCC
AAATAATCAG GAACTGTTTG TCCTTGATGG AACGGAGTTT TCTTTTGCCT
1700
CCCTAACGAC CAACTTGCCT TCCACTATAT ATAGACAAAG GGGTACAGTC
GATTCACTAG ATGTAATACC GCCACAGGAT AATAGTGTAC CACCTCGTGC
1800
GGGATTTAGC CATCGATTGA GTCATGTTAC AATGCTGAGC CAAGCAGCTG
GAGCAGTTTA CACCTTGAGA GCTCAACGT (stop HD-1
(start HD-73) CCT ATGTTCTCTT
GGATACATCG TAGTGCTGAA TTTAATAATA TAATTGCATC GGATAGTATT
1800
ACTCAAATCC CTGCAGTGAA GGGAAACTTT CTTTTTAATG GTTCTGTAAT
TTCAGGACCA GGATTTACTG GTGGGGACTT AGTTAGATTA AATAGTAGTG
1900
GAAATAACAT TCAGAATAGA GGGTATATTG AAGTTCCAAT TCACTTCCCA
TCGACATCTA CCAGATATCG AGTTCGTGTA CGGTATGCTT CTGTAACCCC
2000
GATTCACCTC AACGTTAATT GGGGTAATTC ATCCATTTTT TCCAATACAG
TACCAGCTAC AGCTACGTCA TTAGATAATC TACAATCAAG TGATTTTGGT
2100
TATTTTGAAA GTGCCAATGC TTTTACATCT TCATTAGGTA ATATAGTAGG
TGTTAGAAAT TTTAGTGGGA CTGCAGGAGT GATAATAGAC AGATTTGAAT
2200
TTATTCCAGT TACTGCAACA CTCGAGGCTG AATATAATCT GGAAAGAGCG
CAGAAGGCGG TGAATGCGCT GTTTACGTCT ACAAACCAAC TAGGGCTAAA
2300
AACAAATGTA ACGGATTATC ATATTGATCA AGTGTCCAAT TTAGTTACGT
ATTTATCGGA TGAATTTTGT CTGGATGAAA AGCGAGAATT GTCCGAGAAA
2400
GTCAAACATG CGAAGCGACT CAGTGATGAA CGCAATTTAC TCCAAGATTC
AAATTTCAAA GACATTAATA GGCAACCAGA ACGTGGGTGG GGCGGAAGTA
2500
CAGGGATTAC CATCCAAGGA GGGGATGACG TATTTAAAGA AAATTACGTC
ACACTATCAG GTACCTTTGA TGAGTGCTAT CCAACATATT TGTATCAAAA
2600
AATCGATGAA TCAAAATTAA AAGCCTTTAC CCGTTATCAA TTAAGAGGGT
ATATCGAAGA TAGTCAAGAC TTAGAAATCT ATTTAATTCG CTACAATGCA
2700
AAACATGAAA CAGTAAATGT GCCAGGTACG GGTTCCTTAT GGCCGCTTTC
AGCCCAAAGT CCAATCGGAA AGTGTGGAGA GCCGAATCGA TGCGCGCCAC
2800
ACCTTGAATG GAATCCTGAC TTAGATTGTT CGTGTAGGGA TGGAGAAAAG
TGTGCCCATC ATTCGCATCA TTTCTCCTTA GACATTGATG TAGGATGTAC
2900
AGACTTAAAT GAGGACCTAG GTGTATGGGT GATCTTTAAG ATTAAGACGC
AAGATGGGCA CGCAAGACTA GGGAATCTAG AGTTTCTCGA AGAGAAACCA
3000
TTAGTAGGAG AAGCGCTAGC TCGTGTGAAA AGAGCGGAGA AAAAATGGAG
AGACAAACGT GAAAAATTGG AATGGGAAAC AAATATCGTT TATAAAGAGG
3100
CAAAAGAATC TGTAGATGCT TTATTTGTAA ACTCTCAATA TGATCAATTA
CAAGCGGATA CGAATATTGC CATGATTCAT GCGGCAGATA AACGTGTTCA
3200
TAGCATTCGA GAAGCTTATC TGCCTGAGCT GTCTGTGATT CCGGGTGTCA
ATGCGGCTAT TTTTGAAGAA TTAGAAGGGC GTATTTTCAC TGCATTCTCC
3300
CTATATGATG CGAGAAATGT CATTAAAAAT GGTGATTTTA ATAATGGCTT
ATCCTGCTGG AACGTGAAAG GGCATGTAGA TGTAGAAGAA CAAAACAACC
3400
AACGTTCGGT CCTTGTTGTT CCGGAATGGG AAGCAGAAGT GTCACAAGAA
GTTCGTGTCT GTCCGGGTCG TGGCTATATC CTTCGTGTCA CAGCGTACAA
3500
GGAGGGATAT GGAGAAGGTT GCGTAACCAT TCATGAGATC GAGAACAATA
CAGACGAACT GAAGTTTAGC AACTGCGTAG AAGAGGAAAT CTATCCAAAT
3600
AACACGGTAA CGTGTAATGA TTATACTGTA AATCAAGAAG AATACGGAGG
TGCGTACACT TCTCGTAATC GAGGATATAA CGAAGCTCCT TCCGTACCAG
3700
CTGATTATGC GTCAGTCTAT GAAGAAAAAT CGTATACAGA TGGACGAAGA
GAGAATCCTT GTGAATTTAA CAGAGGGTAT AGGGATTACA CGCCACTACC
3800
AGTTGGTTAT GTGACAAAAG AATTAGAATA CTTCCCAGAA ACCGATAAGG
TATGGATTGA GATTGGAGAA ACGGAAGGAA CATTTATCGT GGACAGCGTG
3900
GAATTACTCC TTATGGAGGA A (end HD-73)
and equivalent nucleotide sequences coding for toxin EW4 with the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F P V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L L A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F S N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G M A Q R I E Q N I R Q P H L M D I L
N S I T I Y T D V H R G F N Y W S G H Q I T A S P V G F S G
P E F A F P L F G N A G N A A P P V L V S L T G L G I F R T
L S S P L Y R R I I L G S G P N N Q E L F V L D G T E F S F
A S L T T N L P S T I Y R Q R G T V D S L D V I P P Q D N S
V P P R A G F S H R L S H V T M L S Q A A G A V Y T L R A Q
R P M F S W I H R S A E F N N I I A S D S I T Q I P A V K G
N F L F N G S V I S G P G F T G G D L V R L N S S G N N I Q
N R G Y I E V P I H F P S T S T R Y R V R V R Y A S V T P I
H L N V N W G N S S I F S N T V P A T A T S L D N L Q S S D
F G Y F E S A N A F T S S L G N I V G V R N F S G T A G V I
I D R F E F I P V T A T L E A E Y N L E R A Q K A V N A L F
T S T N Q L G L K T N V T D Y H I D Q V S N L V T Y L S D E
F C L D E K R E L S E K V K H A K R L S D E R N L L Q D S N
F K D I N R Q P E R G W G G S T G I T I Q G G D D V F K E N
Y V T L S G T F D E C Y P T Y L Y Q K I D E S K L K A F T R
Y Q L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P
G T G S L W P L S A Q S P I G K C G E P N R C A P H L E W N
P D L D C S C R D G E K C A H H S H H F S L D I D V G C T D
L N E D L G V W V I F K I K T Q D G H A R L G N L E F L E E
K P L V G E A L A R V K R A E K K W R D K R E K L E W E T N
I V Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M
I H A A D K R V H S I R E A Y L P E L S V I P G V N A A I F
E E L E G R I F T A F S L Y D A R N V I K N G D F N N G L S
C W N V K G H V D V E E Q N N Q R S V L V V P E W E A E V S
Q E V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H
E I E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y
T V N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S
V Y E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V
G Y V T K E L E Y F P E T D K V W I E I G E T E G T F I V D
S V E L L L M E E.
23 . A pesticidal composition, according to claim 12 , wherein said gene, denoted pACB-1, encoding a chimeric toxin, is as follows:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
AAGAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATAGG GGTTATTATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTG A AT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG A ATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1)
and equivalent nucleotide sequences coding for toxin ACB-1 with the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E R S I R S P H L M D I L
N S I T I Y T D A H R G Y Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L N E I P P Q N N N V
P P R Q E F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E.
24 . A pesticidal composition, according to claim 12 , wherein said gene, denoted pSYW1, encoding a chimeric toxin, is as follows:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
A G GAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATA AA GG GG A A TATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTGGAT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG GATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1)
and equivalent nucleotide sequences coding for toxin SYW1 with the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E G S I R S P H L M D I L
N S I T I Y T D A H K G E Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L D E I P P Q N N N V
P P R Q G F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E.
25 . A recombinant DNA transfer vector comprising DNA having the following nucleotide sequence or equivalent nucleotide sequences containing bases whose translated region codes for the same amino acid sequence:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
AAGAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATAGG GGTTATTATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTGGAT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG GATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1).
26 . A recombinant DNA transfer vector comprising DNA having the following nucleotide sequence or equivalent nucleotide sequences containing bases whose translated region codes for the same amino acid sequence:
start HD-1) ATGG ATAACAATCC GAACATCAAT
GAATGCATTC CTTATAATTG TTTAAGTAAC CCTGAAGTAG AAGTATTAGG
600
TGGAGAAAGA ATAGAAACTG GTTACACCCC AATCGATATT TCCTTGTCGC
TAACGCAATT TCTTTTGAGT GAATTTGTTC CCGGTGCTGG ATTTGTGTTA
700
GGACTAGTTG ATATAATATG GGGAATTTTT GGTCCCTCTC AATGGGACGC
ATTTCCTGTA CAAATTGAAC AGTTAATTAA CCAAAGAATA GAAGAATTCG
800
CTAGGAACCA AGCCATTTCT AGATTAGAAG GACTAAGCAA TCTTTATCAA
ATTTACGCAG AATCTTTTAG AGAGTGGGAA GCAGATCCTA CTAATCCAGC
900
ATTAAGAGAA GAGATGCGTA TTCAATTCAA TGACATGAAC AGTGCCCTTA
CAACCGCTAT TCCTCTTTTG GCAGTTCAAA ATTATCAAGT TCCTCTTTTA
1000
TCAGTATATG TTCAAGCTGC AAATTTACAT TTATCAGTTT TGAGAGATGT
TTCAGTGTTT GGACAAAGGT GGGGATTTGA TGCCGCGACT ATCAATAGTC
1100
GTTATAATGA TTTAACTAGG CTTATTGGCA ACTATACAGA TTATGCTGTG
CGCTGGTACA ATACGGGATT AGAGCGTGTA TGGGGACCGG ATTCTAGAGA
1200
TTGGGTAAGG TATAATCAAT TTAGAAGAGA GCTAACACTT ACTGTATTAG
ATATCGTTGC TCTATTCTCA AATTATGATA GTCGAAGGTA TCCAATTCGA
1300
ACAGTTTCCC AATTAACAAG AGAAATTTAT ACGAACCCAG TATTAGAAAA
TTTTGATGGT AGTTTTCGTG GAATGGCTCA GAGAATAGAA CAGAATATTA
1400
GGCAACCACA TCTTATGGAT ATCCTTAATA GTATAACCAT TTATACTGAT
GTGCATAGAG GCTTTAATTA TTGGTCAGGG CATCAAATAA CAGCTTCTCC
1500
TGTAGGGTTT TCAGGACCAG AATTCGCATT CCCTTTATTT GGGAATGCGG
GGAATGCAGC TCCACCCGTA CTTGTCTCAT TAACTGGTTT GGGGATTTTT
1600
AGAACATTAT CTTCACCTTT ATATAGAAGA ATTATACTTG GTTCAGGCCC
AAATAATCAG GAACTGTTTG TCCTTGATGG AACGGAGTTT TCTTTTGCCT
1700
CCCTAACGAC CAACTTGCCT TCCACTATAT ATAGACAAAG GGGTACAGTC
GATTCACTAG ATGTAATACC GCCACAGGAT AATAGTGTAC CACCTCGTGC
1800
GGGATTTAGC CATCGATTGA GTCATGTTAC AATGCTGAGC CAAGCAGCTG
GAGCAGTTTA CACCTTGAGA GCTCAACGT (stop HD-1)
(start HD-73) CCT ATGTTCTCTT
GGATACATCG TAGTGCTGAA TTTAATAATA TAATTGCATC GGATAGTATT
1800
ACTCAAATCC CTGCAGTGAA GGGAAACTTT CTTTTTAATG GTTCTGTAAT
TTCAGGACCA GGATTTACTG GTGGGGACTT AGTTAGATTA AATAGTAGTG
1900
GAAATAACAT TCAGAATAGA GGGTATATTG AAGTTCCAAT TCACTTCCCA
TCGACATCTA CCAGATATCG AGTTCGTGTA CGGTATGCTT CTGTAACCCC
2000
GATTCACCTC AACGTTAATT GGGGTAATTC ATCCATTTTT TCCAATACAG
TACCAGCTAC AGCTACGTCA TTAGATAATC TACAATCAAG TGATTTTGGT
2100
TATTTTGAAA GTGCCAATGC TTTTACATCT TCATTAGGTA ATATAGTAGG
TGTTAGAAAT TTTAGTGGGA CTGCAGGAGT GATAATAGAC AGATTTGAAT
2200
TTATTCCAGT TACTGCAACA CTCGAGGCTG AATATAATCT GGAAAGAGCG
CAGAAGGCGG TGAATGCGCT GTTTACGTCT ACAAACCAAC TAGGGCTAAA
2300
AACAAATGTA ACGGATTATC ATATTGATCA AGTGTCCAAT TTAGTTACGT
ATTTATCGGA TGAATTTTGT CTGGATGAAA AGCGAGAATT GTCCGAGAAA
2400
GTCAAACATG CGAAGCGACT CAGTGATGAA CGCAATTTAC TCCAAGATTC
AAATTTCAAA GACATTAATA GGCAACCAGA ACGTGGGTGG GGCGGAAGTA
2500
CAGGGATTAC CATCCAAGGA GGGGATGACG TATTTAAAGA AAATTACGTC
ACACTATCAG GTACCTTTGA TGAGTGCTAT CCAACATATT TGTATCAAAA
2600
AATCGATGAA TCAAAATTAA AAGCCTTTAC CCGTTATCAA TTAAGAGGGT
ATATCGAAGA TAGTCAAGAC TTAGAAATCT ATTTAATTCG CTACAATGCA
2700
AAACATGAAA CAGTAAATGT GCCAGGTACG GGTTCCTTAT GGCCGCTTTC
AGCCCAAAGT CCAATCGGAA AGTGTGGAGA GCCGAATCGA TGCGCGCCAC
2800
ACCTTGAATG GAATCCTGAC TTAGATTGTT CGTGTAGGGA TGGAGAAAAG
TGTGCCCATC ATTCGCATCA TTTCTCCTTA GACATTGATG TAGGATGTAC
2900
AGACTTAAAT GAGGACCTAG GTGTATGGGT GATCTTTAAG ATTAAGACGC
AAGATGGGCA CGCAAGACTA GGGAATCTAG AGTTTCTCGA AGAGAAACCA
3000
TTAGTAGGAG AAGCGCTAGC TCGTGTGAAA AGAGCGGAGA AAAAATGGAG
AGACAAACGT GAAAAATTGG AATGGGAAAC AAATATCGTT TATAAAGAGG
3100
CAAAAGAATC TGTAGATGCT TTATTTGTAA ACTCTCAATA TGATCAATTA
CAAGCGGATA CGAATATTGC CATGATTCAT GCGGCAGATA AACGTGTTCA
3200
TAGCATTCGA GAAGCTTATC TGCCTGAGCT GTCTGTGATT CCGGGTGTCA
ATGCGGCTAT TTTTGAAGAA TTAGAAGGGC GTATTTTCAC TGCATTCTCC
3300
CTATATGATG CGAGAAATGT CATTAAAAAT GGTGATTTTA ATAATGGCTT
ATCCTGCTGG AACGTGAAAG GGCATGTAGA TGTAGAAGAA CAAAACAACC
3400
AACGTTCGGT CCTTGTTGTT CCGGAATGGG AAGCAGAAGT GTCACAAGAA
GTTCGTGTCT GTCCGGGTCG TGGCTATATC CTTCGTGTCA CAGCGTACAA
3500
GGAGGGATAT GGAGAAGGTT GCGTAACCAT TCATGAGATC GAGAACAATA
CAGACGAACT GAAGTTTAGC AACTGCGTAG AAGAGGAAAT CTATCCAAAT
3600
AACACGGTAA CGTGTAATGA TTATACTGTA AATCAAGAAG AATACGGAGG
TGCGTACACT TCTCGTAATC GAGGATATAA CGAAGCTCCT TCCGTACCAG
3700
CTGATTATGC GTCAGTCTAT GAAGAAAAAT CGTATACAGA TGGACGAAGA
GAGAATCCTT GTGAATTTAA CAGAGGGTAT AGGGATTACA CGCCACTACC
3800
AGTTGGTTAT GTGACAAAAG AATTAGAATA CTTCCCAGAA ACCGATAAGG
TATGGATTGA GATTGGAGAA ACGGAAGGAA CATTTATCGT GGACAGCGTG
3900
GAATTACTCC TTATGGAGGA A (end HD-73).
27 . A recombinant DNA transfer vector comprising DNA having the following nucleotide sequence or equivalent nucleotide sequences containing bases whose translated region codes for the same amino acid sequence:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
AAGAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATAGG GGTTATTATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTG A AT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG A ATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACACA AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT APTTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1).
28 . A recombinant DNA transfer vector comprising DNA having the following nucleotide sequence or equivalent nucleotide sequences containing bases whose translated region codes for the same amino acid sequence:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
A G GAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATA AA GG GG A A TATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTGGAT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG GATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1).
29 . The DNA transfer vector of claim 25 transferred to and replicated in a prokaryotic or lower eukaryotic microorganism.
30 . The DNA transfer vector of claim 26 transferred to and replicated in a prokaryotic or lower eukaryotic microorganism.
31 . The DNA transfer vector of claim 27 transferred to and replicated in a prokaryotic or lower eukaryotic microorganism.
32 . The DNA transfer vector of claim 28 transferred to and replicated in a prokaryotic or lower eukaryotic microorganism.
33 . Plasmid pEW1 as shown in FIG. 1 of the drawings.
34 . Plasmid pEW2 as shown in FIG. 2 of the drawings.
35 . Plasmid pEW3 as shown in FIG. 3 of the drawings.
36 . Plasmid pEW4 as shown in FIG. 4 of the drawings.
37 . Plasmid pACB-1, having the construction of plasmid pEW3 except that the DNA encoding aspartic acid at position 411 is converted to encode asparagine, and the DNA encoding glycine at position 425 is converted to encode glutamic acid.
38 . Plasmid pSYW1, having the construction of plasmid pEW3 except that the DNA encoding arginine at position 289 is converted to encode glycine, the DNA encoding arginine at position 311 is converted to encode lysine, and the DNA encoding tyrosine at position 313 is converted to encode glutamate.
39 . A microorganism transformed by the transfer vector of claim 25 .
40 . A microorganism transformed by the transfer vector of claim 26 .
41 . A microorganism transformed by the transfer vector of claim 27 .
42 . A microorganism transformed by the transfer vector of claim 28 .
43 . E. coli (pEW3), a microorganism according to claim 39 .
44 . E. coli (pEW4), a microorganism according to claim 40 .
45 . E. coli (pACB-1), a microorganism according to claim 41 .
46 . E. coli (pSYW1), a microorganism according to claim 42 .
47 . A process for preparing pesticidal chimeric toxin EW3 having the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E R S I R S P H L M D I L
N S I T I Y T D A H R G Y Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L D E I P P Q N N N V
P P R Q G F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E
which comprises culturing a prokaryotic microbe hosting a recombinant DNA transfer vector, denoted pEW3, comprising DNA having the following nucleotide sequence or equivalent nucleotide sequences containing bases whose translated region codes for the same amino acid sequence:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
AAGAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATAGG GGTTATTATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTGGAT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG GATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1).
48 . A process for preparing pesticidal chimeric toxin EW4 having the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F P V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L L A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F S N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G M A Q R I E Q N I R Q P H L M D I L
N S I T I Y T D V H R G F N Y W S G H Q I T A S P V G F S G
P E F A F P L F G N A G N A A P P V L V S L T G L G I F R T
L S S P L Y R R I I L G S G P N N Q E L F V L D G T E F S F
A S L T T N L P S T I Y R Q R G T V D S L D V I P P Q D N S
V P P R A G F S H R L S H V T M L S Q A A G A V Y T L R A Q
R P M F S W I H R S A E F N N I I A S D S I T Q I P A V K G
N F L F N G S V I S G P G F T G G D L V R L N S S G N N I Q
N R G Y I E V P I H F P S T S T R Y R V R V R Y A S V T P I
H L N V N W G N S S I F S N T V P A T A T S L D N L Q S S D
F G Y F E S A N A F T S S L G N I V G V R N F S G T A G V I
I D R F E F I P V T A T L E A E Y N L E R A Q K A V N A L F
T S T N Q L G L K T N V T D Y H I D Q V S N L V T Y L S D E
F C L D E K R E L S E K V K H A K R L S D E R N L L Q D S N
F K D I N R Q P E R G W G G S T G I T I Q G G D D V F K E N
Y V T L S G T F D E C Y P T Y L Y Q K I D E S K L K A F T R
Y Q L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P
G T G S L W P L S A Q S P I G K C G E P N R C A P H L E W N
P D L D C S C R D G E K C A H H S H H F S L D I D V G C T D
L N E D L G V W V I F K I K T Q D G H A R L G N L E F L E E
K P L V G E A L A R V K R A E K K W R D K R E K L E W E T N
I V Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M
I H A A D K R V H S I R E A Y L P E L S V I P G V N A A I F
E E L E G R I F T A F S L Y D A R N V I K N G D F N N G L S
C W N V K G H V D V E E Q N N Q R S V L V V P E W E A E V S
Q E V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H
E I E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y
T V N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S
V Y E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V
G Y V T K E L E Y F P E T D K V W I E I G E T E G T F I V D
S V E L L L M E E
which comprises culturing a prokaryotic microbe hosting a recombinant DNA transfer vector, denoted pEW4, comprising DNA having the following nucleotide sequence or equivalent nucleotide sequences containing bases whose translated region codes for the same amino acid sequence:
(start HD-1) ATGG ATAACAATCC GAACATCAAT
GAATGCATTC CTTATAATTG TTTAAGTAAC CCTGAAGTAG AAGTATTAGG
600
TGGAGAAAGA ATAGAAACTG GTTACACCCC AATCGATATT TCCTTGTCGC
TAACGCAATT TCTTTTGAGT GAATTTGTTC CCGGTGCTGG ATTTGTGTTA
700
GGACTAGTTG ATATAATATG GGGAATTTTT GGTCCCTCTC AATGGGACGC
ATTTCCTGTA CAAATTGAAC AGTTAATTAA CCAAAGAATA GAAGAATTCG
800
CTAGGAACCA AGCCATTTCT AGATTAGAAG GACTAAGCAA TCTTTATCAA
ATTTACGCAG AATCTTTTAG AGAGTGGGAA GCAGATCCTA CTAATCCAGC
900
ATTAAGAGAA GAGATGCGTA TTCAATTCAA TGACATGAAC AGTGCCCTTA
CAACCGCTAT TCCTCTTTTG GCAGTTCAAA ATTATCAAGT TCCTCTTTTA
1000
TCAGTATATG TTCAAGCTGC AAATTTACAT TTATCAGTTT TGAGAGATGT
TTCAGTGTTT GGACAAAGGT GGGGATTTGA TGCCGCGACT ATCAATAGTC
1100
GTTATAATGA TTTAACTAGG CTTATTGGCA ACTATACAGA TTATGCTGTG
CGCTGGTACA ATACGGGATT AGAGCGTGTA TGGGGACCGG ATTCTAGAGA
1200
TTGGGTAAGG TATAATCAAT TTAGAAGAGA GCTAACACTT ACTGTATTAG
ATATCGTTGC TCTATTCTCA AATTATGATA GTCGAAGGTA TCCAATTCGA
1300
ACAGTTTCCC AATTAACAAG AGAAATTTAT ACGAACCCAG TATTAGAAAA
TTTTGATGGT AGTTTTCGTG GAATGGCTCA GAGAATAGAA CAGAATATTA
1400
GGCAACCACA TCTTATGGAT ATCCTTAATA GTATAACCAT TTATACTGAT
GTGCATAGAG GCTTTAATTA TTGGTCAGGG CATCAAATAA CAGCTTCTCC
1500
TGTAGGGTTT TCAGGACCAG AATTCGCATT CCCTTTATTT GGGAATGCGG
GGAATGCAGC TCCACCCGTA CTTGTCTCAT TAACTGGTTT GGGGATTTTT
1600
AGAACATTAT CTTCACCTTT ATATAGAAGA ATTATACTTG GTTCAGGCCC
AAATAATCAG GAACTGTTTG TCCTTGATGG AACGGAGTTT TCTTTTGCCT
1700
CCCTAACGAC CAACTTGCCT TCCACTATAT ATAGACAAAG GGGTACAGTC
GATTCACTAG ATGTAATACC GCCACAGGAT AATAGTGTAC CACCTCGTGC
1800
GGGATTTAGC CATCGATTGA GTCATGTTAC AATGCTGAGC CAAGCAGCTG
GAGCAGTTTA CACCTTGAGA GCTCAACGT (stop HD-1)
(start HD-73) CCT ATGTTCTCTT
GGATACATCG TAGTGCTGAA TTTAATAATA TAATTGCATC GGATAGTATT
1800
ACTCAAATCC CTGCAGTGAA GGGAAACTTT CTTTTTAATG GTTCTGTAAT
TTCAGGACCA GGATTTACTG GTGGGGACTT AGTTAGATTA AATAGTAGTG
1900
GAAATAACAT TCAGAATAGA GGGTATATTG AAGTTCCAAT TCACTTCCCA
TCGACATCTA CCAGATATCG AGTTCGTGTA CGGTATGCTT CTGTAACCCC
2000
GATTCACCTC AACGTTAATT GGGGTAATTC ATCCATTTTT TCCAATACAG
TACCAGCTAC AGCTACGTCA TTAGATAATC TACAATCAAG TGATTTTGGT
2100
TATTTTGAAA GTGCCAATGC TTTTACATCT TCATTAGGTA ATATAGTAGG
TGTTAGAAAT TTTAGTGGGA CTGCAGGAGT GATAATAGAC AGATTTGAAT
2200
TTATTCCAGT TACTGCAACA CTCGAGGCTG AATATAATCT GGAAAGAGCG
CAGAAGGCGG TGAATGCGCT GTTTACGTCT ACAAACCAAC TAGGGCTAAA
2300
AACAAATGTA ACGGATTATC ATATTGATCA AGTGTCCAAT TTAGTTACGT
ATTTATCGGA TGAATTTTGT CTGGATGAAA AGCGAGAATT GTCCGAGAAA
2400
GTCAAACATG CGAAGCGACT CAGTGATGAA CGCAATTTAC TCCAAGATTC
AAATTTCAAA GACATTAATA GGCAACCAGA ACGTGGGTGG GGCGGAAGTA
2500
CAGGGATTAC CATCCAAGGA GGGGATGACG TATTTAAAGA AAATTACGTC
ACACTATCAG GTACCTTTGA TGAGTGCTAT CCAACATATT TGTATCAAAA
2600
AATCGATGAA TCAAAATTAA AAGCCTTTAC CCGTTATCAA TTAAGAGGGT
ATATCGAAGA TAGTCAAGAC TTAGAAATCT ATTTAATTCG CTACAATGCA
2700
AAACATGAAA CAGTAAATGT GCCAGGTACG GGTTCCTTAT GGCCGCTTTC
AGCCCAAAGT CCAATCGGAA AGTGTGGAGA GCCGAATCGA TGCGCGCCAC
2800
ACCTTGAATG GAATCCTGAC TTAGATTGTT CGTGTAGGGA TGGAGAAAAG
TGTGCCCATC ATTCGCATCA TTTCTCCTTA GACATTGATG TAGGATGTAC
2900
AGACTTAAAT GAGGACCTAG GTGTATGGGT GATCTTTAAG ATTAAGACGC
AAGATGGGCA CGCAAGACTA GGGAATCTAG AGTTTCTCGA AGAGAAACCA
3000
TTAGTAGGAG AAGCGCTAGC TCGTGTGAAA AGAGCGGAGA AAAAATGGAG
AGACAAACGT GAAAAATTGG AATGGGAAAC AAATATCGTT TATAAAGAGG
3100
CAAAAGAATC TGTAGATGCT TTATTTGTAA ACTCTCAATA TGATCAATTA
CAAGCGGATA CGAATATTGC CATGATTCAT GCGGCAGATA AACGTGTTCA
3200
TAGCATTCGA GAAGCTTATC TGCCTGAGCT GTCTGTGATT CCGGGTGTCA
ATGCGGCTAT TTTTGAAGAA TTAGAAGGGC GTATTTTCAC TGCATTCTCC
3300
CTATATGATG CGAGAAATGT CATTAAAAAT GGTGATTTTA ATAATGGCTT
ATCCTGCTGG AACGTGAAAG GGCATGTAGA TGTAGAAGAA CAAAACAACC
3400
AACGTTCGGT CCTTGTTGTT CCGGAATGGG AAGCAGAAGT GTCACAAGAA
GTTCGTGTCT GTCCGGGTCG TGGCTATATC CTTCGTGTCA CAGCGTACAA
3500
GGAGGGATAT GGAGAAGGTT GCGTAACCAT TCATGAGATC GAGAACAATA
CAGACGAACT GAAGTTTAGC AACTGCGTAG AAGAGGAAAT CTATCCAAAT
3600
AACACGGTAA CGTGTAATGA TTATACTGTA AATCAAGAAG AATACGGAGG
TGCGTACACT TCTCGTAATC GAGGATATAA CGAAGCTCCT TCCGTACCAG
3700
CTGATTATGC GTCAGTCTAT GAAGAAAAAT CGTATACAGA TGGACGAAGA
GAGAATCCTT GTGAATTTAA CAGAGGGTAT AGGGATTACA CGCCACTACC
3800
AGTTGGTTAT GTGACAAAAG AATTAGAATA CTTCCCAGAA ACCGATAAGG
TATGGATTGA GATTGGAGAA ACGGAAGGAA CATTTATCGT GGACAGCGTG
3900
GAATTACTCC TTATGGAGGA A (end HD-73).
49 . A process for preparing pesticidal chimeric toxin ACB-1 having the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E W E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E R S I R S P H L M D I L
N S I T I Y T D A H R G Y Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L N E I P P Q N N N V
P P R Q E F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V R V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E
which comprises culturing a prokaryotic microbe hosting a recombinant DNA transfer vector, denoted pACB-1, comprising DNA having the following nucleotide sequence or equivalent nucleotide sequences containing bases whose translated region codes for the same amino acid sequence:
(start HD-73) ATG GATAACAATC
400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT
500
TTCCTTGTCG CTAACGCAAT TTCTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT
600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA
700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA
800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT
900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG
1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT
1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA
1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
AAGAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA
1300
TCTATACGGA TGCTCATAGG GGTTATTATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA
1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT
1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG
1600
GAACGGTAGA TTCGCTG A AT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG A ATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC
1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT
1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG
2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG
2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT
2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT
2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA
2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT
2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA
2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT
2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA
2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG
2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA
3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA
3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT
3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT
3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA
3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA
3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG
3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT
3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA
3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT
3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA
4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1)
50 . A process for preparing pesticidal chimeric toxin SYW1 having the following amino acid sequence:
M D N N P N I N E C I P Y N C L S N P E V E V L G G E R I E
T G Y T P I D I S L S L T Q F L L S E F V P G A G F V L G L
V D I I W G I F G P S Q W D A F L V Q I E Q L I N Q R I E E
F A R N Q A I S R L E G L S N L Y Q I Y A E S F R E Q E A D
P T N P A L R E E M R I Q F N D M N S A L T T A I P L F A V
Q N Y Q V P L L S V Y V Q A A N L H L S V L R D V S V F G Q
R W G F D A A T I N S R Y N D L T R L I G N Y T D Y A V R W
Y N T G L E R V W G P D S R D W V R Y N Q F R R E L T L T V
L D I V A L F P N Y D S R R Y P I R T V S Q L T R E I Y T N
P V L E N F D G S F R G S A Q G I E G S I R S P H L M D I L
N S I T I Y T D A H K G E Y Y W S G H Q I M A S P V G F S G
P E F T F P L Y G T M G N A A P Q Q R I V A Q L G Q G V Y R
T L S S T L Y R R P F N I G I N N Q Q L S V L D G T E F A Y
G T S S N L P S A V Y R K S G T V D S L D E I P P Q N N N V
P P R Q G F S H R L S H V S M F R S G F S N S S V S I I R A
P T F S W Q H R S A E F N N I I P S S Q I T Q I P L T K S T
N L G S G T S V V K G P G F T G G D I L R R T S P G Q I S T
L R V N I T A P L S Q R Y R V R I R Y A S T T N L Q F H T S
I D G R P I N Q G N F S A T M S S G S N L Q S G S F R T V G
F T T P F N F S N G S S V F T L S A H V F N S G N E V Y I D
R I E F V P A E V T F E A E Y D L E R A Q K A V N E L F T S
S N Q I G L K T D V T D Y H I D Q V S N L V E C L S D E F C
L D E K Q E L S E K V K H A K R L S D E R N L L Q D P N F R
G I N R Q L D R G W R G S T D I T I Q G G D D V F K E N Y V
T L L G T F D E C Y P T Y L Y Q K I D E S K L K A Y T R Y Q
L R G Y I E D S Q D L E I Y L I R Y N A K H E T V N V P G T
G S L W P L S A Q S P I G K C G E P N R C A P H L E W N P D
L D C S C R D G E K C A H H S H H F S L D I D V G C T D L N
E D L G V W V I F K I K T Q D G H A R L G N L E F L E E K P
L V G E A L A R V K R A E K K W R D K R E K L E W E T N I V
Y K E A K E S V D A L F V N S Q Y D Q L Q A D T N I A M I H
A A D K R V H S I R E A Y L P E L S V I P G V N A A I F E E
L E G R I F T A F S L Y D A R N V I K N G D F N N G L S C W
N V K G H V D V E E Q N N Q R S V L V L P E W E A E V S Q E
V P V C P G R G Y I L R V T A Y K E G Y G E G C V T I H E I
E N N T D E L K F S N C V E E E I Y P N N T V T C N D Y T V
N Q E E Y G G A Y T S R N R G Y N E A P S V P A D Y A S V Y
E E K S Y T D G R R E N P C E F N R G Y R D Y T P L P V G Y
V T K E L E Y F P E T D K V W I E I G E T E G T F I V D S V
E L L L M E E
which comprises culturing a prokaryotic microbe hosting a recombinant DNA transfer vector, denoted pSYW1, comprising DNA having the following nucleotide sequence or equivalent nucleotide sequences containing bases whose translated region codes for the same amino acid sequence:
(start HD-73) ATG GATAACAATC 400
CGAACATCAA TGAATGCATT CCTTATAATT GTTTAAGTAA CCCTGAAGTA
GAAGTATTAG GTGGAGAAAG AATAGAAACT GGTTACACCC CAATCGATAT 500
TTCCTTGTCG CTAACGCAAT TTDTTTTGAG TGAATTTGTT CCCGGTGCTG
GATTTGTGTT AGGACTAGTT GATATAATAT GGGGAATTTT TGGTCCCTCT 600
CAATGGGACG CATTTCTTGT ACAAATTGAA CAGTTAATTA ACCAAAGAAT
AGAAGAATTC GCTAGGAACC AAGCCATTTC TAGATTAGAA GGACTAAGCA 700
ATCTTTATCA AATTTACGCA GAATCTTTTA GAGAGTGGGA AGCAGATCCT
ACTAATCCAG CATTAAGAGA AGAGATGCGT ATTCAATTCA ATGACATGAA 800
CAGTGCCCTT ACAACCGCTA TTCCTCTTTT TGCAGTTCAA AATTATCAAG
TTCCTCTTTT ATCAGTATAT GTTCAAGCTG CAAATTTACA TTTATCAGTT 900
TTGAGAGATG TTTCAGTGTT TGGACAAAGG TGGGGATTTG ATGCCGCGAC
TATCAATAGT CGTTATAATG ATTTAACTAG GCTTATTGGC AACTATACAG 1000
ATTATGCTGT ACGCTGGTAC AATACGGGAT TAGAACGTGT ATGGGGACCG
GATTCTAGAG ATTGGGTAAG GTATAATCAA TTTAGAAGAG AATTAACACT 1100
AACTGTATTA GATATCGTTG CTCTGTTCCC GAATTATGAT AGTAGAAGAT
ATCCAATTCG AACAGTTTCC CAATTAACAA GAGAAATTTA TACAAACCCA 1200
GTATTAGAAA ATTTTGATGG TAGTTTTCGA GGCTCGGCTC AGGGCATAGA
A G GAAGTATT AGGAGTCCAC ATTTGATGGA TATACTTAAC AGTATAACCA 1300
TCTATACGGA TGCTCATA AA GG GG A A TATT ATTGGTCAGG GCATCAAATA
ATGGCTTCTC CTGTAGGGTT TTCGGGGCCA GAATTCACTT TTCCGCTATA 1400
TGGAACTATG GGAAATGCAG CTCCACAACA ACGTATTGTT GCTCAACTAG
GTCAGGGCGT GTATAGAACA TTATCGTCCA CTTTATATAG AAGACCTTTT 1500
AATATAGGGA TAAATAATCA ACAACTATCT GTTCTTGACG GGACAGAATT
TGCTTATGGA ACCTCCTCAA ATTTGCCATC CGCTGTATAC AGAAAAAGCG 1600
GAACGGTAGA TTCGCTGGAT GAAATACCGC CACAGAATAA CAACGTGCCA
CCTAGGCAAG GATTTAGTCA TCGATTAAGC CATGTTTCAA TGTTTCGTTC 1700
AGGCTTTAGT AATAGTAGTG TAAGTATAAT AAGAGCT (end hd-73)
(start HD-1) CCAACGT TTTCTTGGCA GCATCGCAGT 1900
GCTGAATTTA ATAATATAAT TCCTTCATCA CAAATTACAC AAATACCTTT
AACAAAATCT ACTAATCTTG GCTCTGGAAC TTCTGTCGTT AAAGGACCAG 2000
GATTTACAGG AGGAGATATT CTTCGAAGAA CTTCACCTGG CCAGATTTCA
ACCTTAAGAG TAAATATTAC TGCACCATTA TCACAAAGAT ATCGGGTAAG 2100
AATTCGCTAC GCTTCTACTA CAAATTTACA ATTCCATACA TCAATTGACG
GAAGACCTAT TAATCAGGGT AATTTTTCAG CAACTATGAG TAGTGGGAGT 2200
AATTTACAGT CCGGAAGCTT TAGGACTGTA GGTTTTACTA CTCCGTTTAA
CTTTTCAAAT GGATCAAGTG TATTTACGTT AAGTGCTCAT GTCTTCAATT 2300
CAGGCAATGA AGTTTATATA GATCGAATTG AATTTGTTCC GGCAGAAGTA
ACCTTTGAGG CAGAATATGA TTTAGAAAGA GCACAAAAGG CGGTGAATGA 2400
GCTGTTTACT TCTTCCAATC AAATCGGGTT AAAAACAGAT GTGACGGATT
ATCATATTGA TCAAGTATCC AATTTAGTTG AGTGTTTATC AGATGAATTT 2500
TGTCTGGATG AAAAACAAGA ATTGTCCGAG AAAGTCAAAC ATGCGAAGCG
ACTTAGTGAT GAGCGGAATT TACTTCAAGA TCCAAACTTC AGAGGGATCA 2600
ATAGACAACT AGACCGTGGC TGGAGAGGAA GTACGGATAT TACCATCCAA
GGAGGCGATG ACGTATTCAA AGAGAATTAC GTTACGCTAT TGGGTACCTT 2700
TGATGAGTGC TATCCAACGT ATTTATATCA AAAAATAGAT GAGTCGAAAT
TAAAAGCCTA TACCCGTTAT CAATTAAGAG GGTATATCGA AGATAGTCAA 2800
GACTTAGAAA TCTATTTAAT TCGCTACAAT GCAAAACATG AAACAGTAAA
TGTGCCAGGT ACGGGTTCCT TATGGCCGCT TTCAGCCCAA AGTCCAATCG 2900
GAAAGTGTGG AGAGCCGAAT CGATGCGCGC CACACCTTGA ATGGAATCCT
GACTTAGATT GTTCGTGTAG GGATGGAGAA AAGTGTGCCC ATCATTCGCA 3000
TCATTTCTCC TTAGACATTG ATGTAGGATG TACAGACTTA AATGAGGACC
TAGGTGTATG GGTGATCTTT AAGATTAAGA CGCAAGATGG GCACGCAAGA 3100
CTAGGGAATC TAGAGTTTCT CGAAGAGAAA CCATTAGTAG GAGAAGCGCT
AGCTCGTGTG AAAAGAGCGG AGAAAAAATG GAGAGACAAA CGTGAAAAAT 3200
TGGAATGGGA AACAAATATC GTTTATAAAG AGGCAAAAGA ATCTGTAGAT
GCTTTATTTG TAAACTCTCA ATATGATCAA TTACAAGCGG ATACGAATAT 3300
TGCCATGATT CATGCGGCAG ATAAACGTGT TCATAGCATT CGAGAAGCTT
ATCTGCCTGA GCTGTCTGTG ATTCCGGGTG TCAATGCGGC TATTTTTGAA 3400
GAATTAGAAG GGCGTATTTT CACTGCATTC TCCCTATATG ATGCGAGAAA
TGTCATTAAA AATGGTGATT TTAATAATGG CTTATCCTGC TGGAACGTGA 3500
AAGGGCATGT AGATGTAGAA GAACAAAACA ACCAACGTTC GGTCCTTGTT
CTTCCGGAAT GGGAAGCAGA AGTGTCACAA GAAGTTCGTG TCTGTCCGGG 3600
TCGTGGCTAT ATCCTTCGTG TCACAGCGTA CAAGGAGGGA TATGGAGAAG
GTTGCGTAAC CATTCATGAG ATCGAGAACA ATACAGACGA ACTGAAGTTT 3700
AGCAACTGCG TAGAAGAGGA AATCTATCCA AATAACACGG TAACGTGTAA
TGATTATACT GTAAATCAAG AAGAATACGG AGGTGCGTAC ACTTCTCGTA 3800
ATCGAGGATA TAACGAAGCT CCTTCCGTAC CAGCTGATTA TGCGTCAGTC
TATGAAGAAA AATCGTATAC AGATGGACGA AGAGAGAATC CTTGTGAATT 3900
TAACAGAGGG TATAGGGATT ACACGCCACT ACCAGTTGGT TATGTGACAA
AAGAATTAGA ATACTTCCCA GAAACCGATA AGGTATGGAT TGAGATTGGA 4000
GAAACGGAAG GAACATTTAT CGTGGACAGC GTGGAATTAC TCCTTATGGA
GGAA (end HD-1).
51 . A chimeric toxin, having the amino acid sequence of toxin EW3, with changes which can be shown schematically as follows:
wherein X is one of the 20 common amino acids except Asp when the amino acid at position 425 is Gly; Y is one of the 20 common amino acids except Gly when the amino acid at position 411 is Asp.
52 . A chimeric toxin, having the amino acid sequence of toxin EW3, with changes which can be shown schematically as follows:
wherein X is one of the 20 common amino acids except Arg when the amino acid at position 311 is Arg and the amino acid at position 313 is Tyr; Y is one of the 20 common amino acids except Arg when the amino acid at position 289 is Arg and the amino acid at position 313 is Tyr; and Z is one of the 20 common amino acids except Tyr when the amino acid at position 289 is Arg and the amino acid at position 311 is Arg.
53 . DNA encoding a chimeric toxin as shown in claim 51 .
54 . DNA encoding a chimeric toxin as shown in claim 52 .
55 . A recombinant DNA transfer vector comprising DNA encoding a chimeric toxin as shown in claim 51 .
56 . A recombinant DNA transfer vector comprising DNA encoding a chimeric toxin as shown in claim 52 .
57 . A chimeric toxin comprising the variable region or regions of two or more Bacillus toxins.
58 . A toxin, according to claim 57 , wherein the Bacillus toxins are B. thuringiensis toxins.
59 . A toxin, according to claim 58 , wherein the B. thuringiensis toxins are B. thuringiensis var. kurstaki HD-1 toxin and B. thuringiensis var. kurstaki HD-73 toxin.
60 . A toxin, according to claim 58 , wherein the B. thuringiensis toxins are encoded by a pesticide-producing strain of Bacillius thuringiensis , consisting of B. thuringiensis M-7, B. thuringiensis var. kurstaki, B. thuringiensis var. finitimus, B. thuringiensis var. alesti, B. thuringiensis var. sotto, B. thuringiensis var. dendrolimus, B. thuringiensis var. kenyae, B. thuringiensis var. galleriae, B. thuringiensis var. canadensis, B. thuringiensis var. entomocidus, B. thuringiensis var. subtoxicus, B. thuringiensis var. aizawai, B. thuringiensis var. morrisoni, B. thuringiensis var. ostriniae, B. thuringiensis var. tolworthi, B. thuringiensis var. darmstadiensis, B. thuringiensis var. toumanoffi, B. thuringiensis var. kyushuensis, B. thuringiensis var. thompsoni, B. thuringiensis var. pakistani, B. thuringiensis var. israelensis, B. thuringiensis var. indiana, B. thuringiensis var. dakota, B. thuringiensis var. tohokuensis, B. thuringiensis var. kumanotoensis, B. thuringiensis var. tochigiensis, B. thuringiensis var. colmeri, B. thuringiensis var. wuhanensis, B. thuringiensis var. tenebrionis, B. thuringiensis var. thuringiensis, and other Bacillus species selected from B. cereus, B. moritai, B. popilliae, B. lentimorbus, and B. sphaericus.Join the waitlist — get patent alerts
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