Active slide and the preparation method thereof

Abstract

The invention discloses a method for preparing an active slide, including introducing a monomer containing an aldehyde group, or a mixture of a monomer containing an aldehyde group and an acidic functional group provider into a plasma chamber; and depositing the aldehyde group and acidic functional group onto the surface of an organic or inorganic matrix using plasma deposition to form a slide comprising a layer of polymerized actively functional groups thereon. The aldehyde groups and negatively charged groups are deposited on the surface of the active slide, such that the bio-molecules bound thereto possess the properties of an inducible orientation and thus form a mono-layer.

What is claimed is:  
     
         1 . A method for preparing an active slide, comprising: 
 (a) introducing a monomer containing an aldehyde group, or a mixture of a monomer containing an aldehyde group and an acidic functional group provider into a plasma chamber; and    (b) depositing said aldehyde group and acidic functional group onto the surface of an organic matrix using plasma deposition to form a slide comprising a layer of polymerized actively functional groups thereon.    
     
     
         2 . The method as claimed in  claim 1 , further comprising cleaning said organic matrix and plasma chamber before step (a).  
     
     
         3 . A method for preparing an active slide, comprising: 
 (a) introducing an organic compound containing silicon into a plasma chamber and forming an interlayer on the surface of an inorganic matrix using plasma deposition; and    (b) introducing a mixture of a monomer containing an aldehyde group and an acidic functional group provider into the plasma chamber, and depositing said aldehyde group and acidic functional group onto the surface of said interlayer using plasma deposition to form a slide comprising a layer of polymerized actively functional groups thereon.    
     
     
         4 . The method as claimed in  claim 3 , further comprising cleaning said inorganic matrix and plasma chamber before step (a).  
     
     
         5 . The method as claimed in  claim 1 , wherein said organic matrix comprises a polymer polymerized by an organic molecule, wherein said organic molecule is selected from the group consisting of a monomer of ethylene, propylene, ester, acrylic acid, acrylate, alkyl acrylic acid and alkyl acrylate.  
     
     
         6 . The method as claimed in  claim 3 , wherein said inorganic matrix comprises silicon wafer, ceramic material, glass or metal.  
     
     
         7 . The method as claimed in any one of  claim 2  and  claim 4 , wherein the cleaning step is performed by the pretreatment with an solvent and/or sonication.  
     
     
         8 . The method as claimed in  claim 7 , wherein said solvent is selected from the group consisting of surfactant, water, alcohol and acetone.  
     
     
         9 . The method as claimed in  claim 3 , wherein said organic compound containing silicon comprises the volatile monomer of hexamethyl disilazane or hexamethyl disiloxane.  
     
     
         10 . The method as claimed in any one of  claim 1  and  claim 3 , wherein said monomer containing an aldehyde group comprises the volatile monomer of acrolein or benzaldehyde.  
     
     
         11 . The method as claimed in any one of  claim 1  and  claim 3 , wherein said acidic functional group comprises carboxyl group, phosphate group or sulfonate group.  
     
     
         12 . An active slide, comprising: 
 (i) an organic matrix; and    (ii) a layer of actively functional groups polymerized by a monomer containing an aldehyde group using plasma deposition, wherein said layer is deposited onto said organic matrix.    
     
     
         13 . An active slide, comprising: 
 (i) an inorganic matrix;    (ii) an interlayer polymerized by a monomer of an organic compound containing silicon using plasma deposition, wherein said interlayer is deposited onto said inorganic matrix; and    (iii) a layer of actively functional groups polymerized by a monomer containing an aldehyde group using plasma deposition, wherein said layer is deposited onto said interlayer.    
     
     
         14 . An active slide with a negatively charged surface, comprising: 
 (i) an organic matrix; and    (ii) a layer of actively functional groups polymerized by a mixture of a monomer containing an aldehyde group and an acidic functional group provider using plasma deposition, wherein said layer is deposited onto said organic matrix.    
     
     
         15 . An active slide with a negatively charged surface, comprising: 
 (i) an inorganic matrix;    (ii) an interlayer polymerized by a monomer of an organic compound containing silicon using plasma deposition, wherein said interlayer is deposited onto said inorganic matrix; and    (iii) a layer of actively functional groups polymerized by a mixture of a monomer containing an aldehyde group and an acidic functional group provider using plasma deposition, wherein said layer is deposited onto said interlayer.    
     
     
         16 . The active slide as claimed in any one of  claim 12  and  claim 14 , wherein said organic matrix comprises a polymer polymerized by an organic molecule, wherein said organic molecule is selected from the group consisting of a monomer of ethylene, propylene, ester, acrylic acid, acrylate, alkyl acrylic acid and alkyl acrylate.  
     
     
         17 . The active slide as claimed in any one of  claim 13  and  claim 15 , wherein said inorganic matrix comprises silicon wafer, ceramic material, glass or metal.  
     
     
         18 . The active slide as claimed in any one of  claim 13  and  claim 15 , wherein said organic compound containing silicon comprises the volatile monomer of hexamethyl disilazane or hexamethyl disiloxane.  
     
     
         19 . The active slide as claimed in any one of claims  12 - 15 , wherein said monomer containing an aldehyde group comprises the volatile monomer of acrolein or benzaldehyde.  
     
     
         20 . The active slide as claimed in any one of  claim 14  and  claim 15 , wherein said acidic functional group comprises carboxyl group, phosphate group or sulfonate group.  
     
     
         21 . An active slide microarray, comprising: 
 (i) an organic matrix;    (ii) a layer of actively functional groups polymerized by a monomer containing an aldehyde group using plasma deposition, wherein said layer is deposited onto said organic matrix; and    (iii) a biologically active material, which is immobilized onto said layer of actively functional groups.    
     
     
         22 . An active slide microarray, comprising: 
 (i) an inorganic matrix;    (ii) an interlayer polymerized by a monomer of an organic compound containing silicon using plasma deposition, wherein said interlayer is deposited onto said inorganic matrix;    (iii) a layer of actively functional groups polymerized by a monomer containing an aldehyde group using plasma deposition, wherein said layer is deposited onto said interlayer; and    (iv) a biologically active material, which is immobilized onto said layer of actively functional groups.    
     
     
         23 . An active slide microarray with a negatively charged surface, comprising: 
 (i) an organic matrix;    (ii) a layer of actively functional groups polymerized by a mixture of a monomer containing an aldehyde group and an acidic functional group provider using plasma deposition, wherein said layer is deposited onto said organic matrix; and    (iii) a biologically active material, which is immobilized onto said layer of actively functional groups.    
     
     
         24 . An active slide microarray with a negatively charged surface, comprising: 
 (i) an inorganic matrix;    (ii) an interlayer polymerized by a monomer of an organic compound containing silicon using plasma deposition, wherein said interlayer is deposited onto said inorganic matrix;    (iii) a layer of actively functional groups polymerized by a mixture of a monomer containing an aldehyde group and an acidic functional group provider using plasma deposition, wherein said layer is deposited onto said interlayer; and    (iv) a biologically active material, which is immobilized onto said layer of actively functional groups.    
     
     
         25 . The microarray as claimed in any one of  claim 21  and  claim 23 , wherein said organic matrix comprises a polymer polymerized by an organic molecule, wherein said organic molecule is selected from the group consisting of a monomer of ethylene, propylene, ester, acrylic acid, acrylate, alkyl acrylic acid and alkyl acrylate.  
     
     
         26 . The microarray as claimed in any one of  claim 22  and  claim 24 , wherein said inorganic matrix comprises silicon wafer, ceramic material, glass or metal.  
     
     
         27 . The microarray as claimed in any one of  claim 22  and  claim 24 , wherein said organic compound containing silicon comprises the volatile monomer of hexamethyl disilazane or hexamethyl disiloxane.  
     
     
         28 . The microarray as claimed in any one of claims  21 - 24 , wherein said monomer containing an aldehyde group comprises the volatile monomer of acrolein or benzaldehyde.  
     
     
         29 . The microarray as claimed in any one of  claim 23  or  claim 24 , wherein said acidic functional group comprises carboxyl group, phosphate group or sulfonate group.  
     
     
         30 . The microarray as claimed in any one of claims  21 - 24 , wherein said biologically active material comprises nucleic acid, oligonucleotide, peptide nucleic acid, antigen, antibody, enzyme or protein.  
     
     
         31 . The microarray as claimed in any one of  claim 23  or  claim 24 , wherein said biologically active material is characterized by having an inducible orientation and forming a mono-layer.