Phytochrome regulated transcription factor for control of higher plant development

Abstract

The present invention involves the isolation and characterization of the first discovered phytochrome-regulated transcriptional factor, a protein designated CCA1 which binds to the promoter region of the chlorophyll binding protein gene (Lhcb1*3) of Arabidopsis. The Lhcb1*3 gene of Arabidopsis is known to be regulated by phytochrome in etiolated seedlings where a brief illumination by red light results in a large increase in the level of mRNA from this gene. A DNA binding activity, designated CA-1, that interacts with the promoter region of Lhcb1*3 was previously discovered in cellular extracts. This binding activity was used to obtain a cDNA clone for a transcription factor that binds specifically to the Lhcb1*3 promoter. Modification of the expression of CCA1 using techniques of genetic engineering results in unexpected changes in the timing of plant flowering. When CCA1 is overexpressed, it appears that the normal circadian rhythms of the plant are disrupted. The plants take a significantly longer time to reach flowering even in the presence of day length conditions that normally induce flowering. Thus, a method of extending vegetative growth and delaying flowering is provided.

What is claimed is:  
     
         1 . A transcription factor comprising a member selected from the group consisting of: 
 (a) a peptide having an amino acid sequence of SEQ ID NO:2;    (b) a peptide having an amino acid sequence identical to a peptide produced by translation of coding portions of nucleic acid sequence Seq ID NO:1;    (c) a peptide having an amino acid sequence identical to a peptide produced by translation of nucleic acid sequence SEQ NO:3;    (d) a peptide having at least 95% sequence homology to peptide (a).    
     
     
         2 . An isolated polynucleotide comprising a member selected from the group consisting of: 
 (a) a polynucleotide having a sequence identical to SEQ ID NO:1; and    (b) a polynucleotide which hybridizes to and which is at least 95% complementary to polynucleotide (a); and    (c) a polynucleotide that is exactly complementary to polynucleotide (b).    
     
     
         3 . An isolated polynucleotide comprising a member selected from the group consisting of: 
 (a) a polynucleotide having a sequence identical to SEQ ID NO:3;    (b) a polynucleotide which hybridizes to and which is at least 95% complementary to polynucleotide (a); and    (c) a polynucleotide that is exactly complementary to polynucleotide (b)    
     
     
         4 . A method of altering plant development comprising transforming a plant with nucleic acid sequence selected from the group consisting of: 
 (a) a polynucleotide having a sequence identical to SEQ ID NO:1;    (b) a polynucleotide which hybridizes to and which is at least 95% complementary to polynucleotide (a)    (c) a polynucleotide having a sequence identical to SEQ ID NO:3; and    (d) a polynucleotide which hybridizes to and which is at least 95% complementary to polynucleotide (c).    
     
     
         5 . A transgenic plant produced by transforming a plant with a nucleic acid sequence selected from the group consisting of: 
 (a) a polynucleotide having a sequence identical to SEQ ID NO:1;    (b) a polynucleotide which hybridizes to and which is at least 95% complementary to polynucleotide (a)    (c) a polynucleotide having a sequence identical to SEQ ID NO:3; and    (d) a polynucleotide which hybridizes to and which is at least 95% complementary to polynucleotide (c).    
     
     
         6 . A method of altering plant development comprising transforming a plant with a nucleic acid sequence coding for a CCA1 protein, said protein having a domain showing at least 85% homology to amino acids 24-75 of SEQ ID NO:2.  
     
     
         7 . A transgenic plant transformed with a nucleic acid sequence coding for a CCA1 protein, said protein having a domain showing at least 85% homology to amino acids 24-75 of SEQ ID NO:2.