Method for producing micro-carrier and test method by using said micro-carrier

Abstract

The invention provides a method for producing a microcarrier, which includes patterning pluralities of bar code on a mask; exposing the bar code to a substrate coated with photoresist; etching and removing residual photoresist and electroforming to a nickel plate; placing a bead coated with biotin or poly-L-lysine between two-nickel plates, and compressing the bar code on the surface of the bead to form a microcake-like particle with bar code; and combining the is particle with the corresponding bio-molecule thereof to produce a micro-carrier with a label. The invention also provides a test method for identifying a bio-molecule, which includes mixing several micro-carriers with the labeled unknown bio-molecules; and identifying the bar code on the micro-carrier via image recognition system, wherein the numbers and types of the known micro-carrier can be flexibly adjusted.

What is claimed is:  
     
         1 . A method for identifying unknown target bio-molecule, comprising: 
 providing a plurality of micro-carriers with known bio-molecules and codes;    labeling unknown target bio-molecule;    mixing said micro-carriers with said labeled unknown target bio-molecules to allow known bio-molecule on the micro-carrier to react with the labeled unknown target bio-molecule; and    identifying the labeled unknown target bio-molecule by analyzing the code on the micro-carrier that has bound with the labeled unknown target bio-molecule.    
     
     
         2 . A method as claimed in  claim 1 , wherein said micro-carrier refers to a substrate with a specific code that corresponds with a known bio-molecule.  
     
     
         3 . A method as claimed in  claim 2 , wherein the material of said substrate includes silicon, glass, plant cellulose, gel, and organic polymers.  
     
     
         4 . A method as claimed in  claim 1 , wherein said code can use bar code attached on a micro-carrier to identify the unknown bio-molecule.  
     
     
         5 . A method as claimed in  claim 1 , wherein said code can also use the shape, size, or color of the micro-carrier as a code to identify the unknown bio-molecule.  
     
     
         6 . A method as claimed in  claim 1 , wherein the function of the code is for identifying the unknown bio-molecule.  
     
     
         7 . A method as claimed in  claim 1 , wherein said bio-molecule comprises nucleic acid, oligonucleotide, peptide nucleic acid, antigen, antibody, enzyme or protein.  
     
     
         8 . A method as claimed in  claim 1 , wherein the step of labeling the unknown target bio-molecules can use fluorescence-labeled.  
     
     
         9 . A method as claimed in  claim 1 , wherein an image recognition system can be used to recognize the code on the micro-carrier whereby the unknown bio-molecule is identified.  
     
     
         10 . A method as claimed in  claim 9 , wherein said image recognition system includes a microscope or a CCD camera.  
     
     
         11 . A method as claimed in  claim 1 , wherein the each code on a micro-carrier uniquely corresponds to at least one known bio-molecule attached on the micro-carrier.