US2003083464A1PendingUtilityA1

Novel peptides comprising repetitive units of amino acids and DNA sequences encoding the same

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Assignee: PROTEIN POLYMER TECH INCPriority: Nov 4, 1986Filed: Mar 12, 2002Published: May 1, 2003
Est. expiryNov 4, 2006(expired)· nominal 20-yr term from priority
Inventors:Franco FerrariCharles C. RichardsonJames ChambersStuart CauseyThomas J. PollockJoseph CappelloJohn W. Crissman
C12N 15/75C12N 15/66C12N 15/70C07K 14/43586C12N 15/11C12N 15/62C07K 2319/33C07K 14/78C12N 1/06C07K 2319/61C07K 2319/00C07K 2319/02
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Claims

Abstract

Novel polypeptides comprising repetitive units of amino acids, as well as synthetic genes encoding the subject polypeptides are provided. The subject polypeptides are characterized by comprising repetitive units of amino acids, where the repetitive units are present in naturally occurring proteins, particularly naturally occurring structural proteins. The subject polypeptides find use in a variety of applications, such as structural components of prosthetic devices, synthetic fibers, and the like.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A protein of at least 30 kDal comprising at least 50 number percent of amino acids of at least one naturally occurring repeating unit of a naturally occurring structural protein and of from 3 to 20 amino acids, each of the same said repeating unit having the same amino acids.  
     
     
         2 . A protein according to  claim 1 , wherein said repeating unit is of from 3 to 15 amino acids.  
     
     
         3 . A protein according to  claim 1 , wherein said repeating unit is of from 3 to 9 amino acids.  
     
     
         4 . A protein according to  claim 1 , wherein said repetitive units are in blocks of at least 2 repetitive units.  
     
     
         5 . A protein according to  claim 1 , wherein said protein comprises an intervening group of from 1 to 50 amino acids between said blocks of repetitive units.  
     
     
         6 . A protein of at least 30 kDal comprising at least 50 number percent of amino acids of at least one naturally occurring repeating unit of a naturally occurring structural protein selected from the group consisting of collagen, elastin, silk, and keratin and of from 3 to 20 amino acids, each of the same said repeating unit having the same amino acids.  
     
     
         7 . A protein according to  claim 6 , wherein said repetitive units are in blocks of at least 2 repetitive units.  
     
     
         8 . A protein according to  claim 6 , wherein said protein comprises an intervening group of from 1 to 50 amino acids between said blocks of repetitive units.  
     
     
         9 . The polypeptide according to  claim 6 , wherein said repeating unit is selected from the-group consisting of SGAGAG (SEQ ID NO:21) and GAGAGS (SEQ ID NO:41).  
     
     
         10 . A protein according to  claim 6 , wherein said repeating unit is selected from the group consisting of GVGVP (SEQ ID NO:23), VPGVG (SEQ ID NO:24) APGVGV (SEQ ID NO:25).  
     
     
         11 . A protein according to  claim 6 , wherein said repeating unit is Gap, wherein a and P are any amino acid, a and P being selected so that the encoded protein comprises from about 10 to 45 number % proline.  
     
     
         12 . A protein according to  claim 6 , wherein said repeating unit is K-L-(1)-L-A-E-A (SEQ ID NO:105), wherein 1 is a basic or acidic amino acid, particularly K or E and the repeating units alternate as to whether 1 is a basic or acidic amino acid.  
     
     
         13 . A protein of at least 30 kDal comprising at least 50 number percent of amino acids of at least one naturally occurring repeating unit of a naturally occurring structural protein and of from 3 to 20 amino acids, each of the same said repeating unit having the same amino acids, further comprising intervening groups of from 1 to 50 amino acids between regions of at least 2 repeating units.  
     
     
         14 . A DNA sequence encoding a protein according to  claim 1 .  
     
     
         15 . A DNA sequence encoding a protein according to  claim 6 .  
     
     
         16 . A DNA sequence encoding a protein according to  claim 13 .  
     
     
         17 . A cell comprising a DNA sequence according to  claim 14 .  
     
     
         18 . A formed object comprising a protein according to  claim 1 .  
     
     
         19 . A formed object comprising a protein according to  claim 6 .  
     
     
         20 . A formed object comprising a protein according to  claim 1.

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