US2003093831A1PendingUtilityA1
Methods of using viral replicase polynucleotides and polypeptides
Est. expiryFeb 25, 2019(expired)· nominal 20-yr term from priority
Inventors:William J. Gordon-KammKeith LoweMatthew A. BaileyCarolyn A. GregoryGoerge HoersterBrian LarkinsBrian DilkesRonald BurnettYoung Min Woo
C12N 15/8209C12N 15/8261C12N 15/8201C12N 15/8207Y02A40/146
57
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Claims
Abstract
The invention provides novel methods of using viral replicase polypeptides. Included are methods for increasing transformation frequencies, providing a positive growth advantage, and modulating cell division.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1 . A method for increasing transformation frequencies in a target plant cell comprising introducing into the target plant cell an isolated plant geminivirus replicase polypeptide.
2 . The method of claim 2 wherein the geminivirus replicase polypeptide is wheat dwarf virus Replicase.
3 . The method of claim 3 wherein the geminivirus replicase polypeptide is RepA.
4 . The method of claim 1 further comprising introducing into the target plant cell a polynucleotide of interest.
5 . The method of claim 4 wherein the plant cell is from a monocot or a dicot plant.
6 . The method of claim 5 wherein the plant cell is from corn, soybean, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, potato, tomato, and millet.
7 . A method for providing a positive growth advantage in a target plant cell comprising introducing into the target plant cell an isolated plant geminivirus replicase polypeptide.
8 . The method of claim 7 wherein the geminivirus replicase polypeptide is wheat dwarf virus Replicase.
9 . The method of claim 8 wherein the geminivirus replicase polypeptide is RepA.
10 . The method of claim 7 wherein the plant cell is a monocot or a dicot.
11 . The method of claim 10 wherein the plant cell is from corn, soybean, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, potato, tomato, and millet.
12 . A method for modulating cell division of a target plant cell capable of dividing, comprising introducing into the target plant cell an isolated viral replicase polypeptide.
13 . The method of claim 12 wherein the polypeptide is wheat dwarf virus Replicase.
14 . The method of claim 13 wherein the polypeptide is RepA.
15 . The method of claim 12 wherein the plant cell is from a monocot or a dicot plant.
16 . The method of claim 15 wherein the plant cell is from corn, soybean, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, potato, tomato, and millet.Join the waitlist — get patent alerts
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