Method for tissue culture in vitro

Abstract

The method for tissue culture in vitro of the present invention is to partially digest the tissue blocks via enzyme, and then seed the partially digested tissue blocks into the hollow cavity of the porous chamber, wherein tissue grow three-dimensionally inside-out toward the surrounding pores of the porous chamber, thus new tissue is proliferated directly with the original tissue form, a method that cultures tissue needed in vitro by using the smallest quantity of tissue within the shortest period of time.

What is claimed is:  
     
         1 . A method for tissue culture in vitro, comprising steps as follows: providing a porous chamber with hollow cavity; digesting partially the tissue blocks via enzyme; 
 seeding partially enzyme-digested tissue blocks and the tissue cells obtained after digestion in said hollow cavity of said porous chamber; and    placing said porous chamber containing said tissue blocks and said tissue cells in the culture medium to conduct the culture process.    
     
     
         2 . A method for tissue culture in vitro as in  claim 1 , wherein said porous chamber is characterized in that said porous chamber contains at least one hollow cavity for the purpose of placing said tissue blocks.  
     
     
         3 . A method for tissue culture in vitro as in  claim 1 , wherein the diameters of said tissue blocks are larger than the pore diameters of the scaffold in the periphery of said hollow cavity of said porous chamber.  
     
     
         4 . A method for tissue culture in vitro as in  claim 1 , wherein the diameters of said tissue blocks are from 500 to 1000 μm.  
     
     
         5 . A method for tissue culture in vitro as in  claim 1 , wherein the shapes of said porous chamber can be cylindrical, cube or any other kinds of shape.  
     
     
         6 . A method for tissue culture in vitro as in  claim 1 , wherein the range for the pore diameters of said porous chamber is from 50 to 500 μm.  
     
     
         7 . A method for tissue culture in vitro as in  claim 1 , wherein said porous chamber is made of biologically absorbable polymer material chosen from groups as follows: polyglycolic acid (PGA), polylactic acid (PLA), poly (glycolic co-Lactic) acid (PLGA), polyanhydrides, polycapralactone, polydioxanone and polyorthoester.  
     
     
         8 . A method for tissue culture in vitro as in  claim 7 , wherein the better material for said porous chamber is poly (glycolic co-Lactic) acid (PLGA).  
     
     
         9 . A method for tissue culture in vitro as in  claim 1 , wherein said tissue blocks can be of any animal tissue, e.g., cartilage tissue.  
     
     
         10 . A method for tissue culture in vitro as in  claim 1 , wherein said enzyme can be collagenase, or any other enzymes whereby tissue block cells can be digested.  
     
     
         11 . A method for tissue culture in vitro as in  claim 1 , wherein said culture medium is the one containing fetal calf serum.  
     
     
         12 . A porous chamber, comprising: 
 a main body;    at least one hollow cavity having an aperture, located in the interior of said main body; and    an upper cover, used for covering said aperture of said hollow cavity.    
     
     
         13 . A porous chamber as in  claim 12 , wherein the shape of said main body can be cylindrical, cube or any other kinds of shape.  
     
     
         14 . A porous chamber as in  claim 12 , wherein the range for the pore diameters of said porous chamber is from 50 to 500 μm.  
     
     
         15 . A porous chamber as in  claim 12 , wherein said porous chamber is made of biologically absorbable polymer material which is chosen from groups as follows: polyglycolic acid (PGA), polylactic acid (PLA), poly (glycolic co-Lactic) acid (PLGA), polyanhydrides, polycapralactone, polydioxanone and polyorthoester.  
     
     
         16 . A porous chamber as in  claim 15 , wherein the better material for said porous chamber is poly (glycolic co-Lactic) acid (PLGA).  
     
     
         17 . A porous chamber as in  claim 12 , wherein said upper cover is made of porous material identical to that of said main body.