Cyclin D polynucleotides polypeptides and uses thereof

Abstract

The invention provides isolated polynucleotides and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants.

What is claimed is:  
     
         1 . An isolated protein having cell cycle accelerating activity comprising: 
 (a) a polypeptide comprising at least 25 contiguous amino acids of SEQ ID NOS: 2, 12, 14, or 22;    (b) a polypeptide which is a monocot cyclin D protein;    (c) a polypeptide comprising at least 85% sequence identity to SEQ ID NOS: 2, 12, 14, or 22, wherein the % sequence identity is based on the entire sequence and is determined by GAP 10 using default parameters;    (d) a polypeptide encoded by a nucleic acid of SEQ ID NOS: 1, 11, 13, or 21;    (e) a polypeptide comprising a first domain of the plant CycD specific binding motif LXCXE; a second domain of a WILKV motif; and a third domain of a cyclin box;    (f) a polypeptide encoded by the polynucleotide having the sequence of ATCC deposit having the Designation No. 98848 or 98847; or    (g) a polypeptide characterized by SEQ ID NO: 2, 12, 14, or 22.    
     
     
         2 . The protein of  claim 1 , wherein the polypeptide is catalytically active.  
     
     
         3 . A host cell comprising the isolated protein of  claim 1 .  
     
     
         4 . The host cell of  claim 3  that is a plant cell.  
     
     
         5 . The plant cell of  claim 4  that is a transgenic cell.  
     
     
         6 . A ribonucleic acid sequence encoding the isolated protein of  claim 1 .  
     
     
         7 . An isolated protein having RB phosphorylation activity comprising: 
 (a) a polypeptide comprising at least 25 contiguous amino acids of SEQ ID NOS: 2, 12, 14, or 22;    (b) a polypeptide which is a monocot cyclin D protein;    (c) a polypeptide comprising at least 85% sequence identity to SEQ ID NOS: 2, 12, 14, or 22, wherein the % sequence identity is based on the entire sequence and is determined by GAP 10 using default parameters;    (d) a polypeptide encoded by a nucleic acid of SEQ ID NOS: 1, 11, 13, or 21;    (e) a polypeptide comprising a first domain of the plant CycD specific binding motif LXCXE; a second domain of a WILKV motif; and a third domain of a cyclin box;    (f) a polypeptide encoded by the polynucleotide having the sequence of ATCC deposit having the Designation No. 98848 or 98847; or    (g) a polypeptide characterized by SEQ ID NO: 2, 12, 14, or 22.    
     
     
         8 . A host cell comprising the isolated protein of  claim 7 .  
     
     
         9 . The host cell of  claim 8  that is a plant cell.  
     
     
         10 . The plant cell of  claim 9  that is a transgenic cell.  
     
     
         11 . A ribonucleic acid sequence encoding the isolated protein of  claim 7 .  
     
     
         12 . The polypeptide of  claim 7 , wherein the polypeptide further comprises cell cycle accelerating activity.  
     
     
         13 . An isolated polypeptide having transformation efficiency activity comprising: 
 (a) a polypeptide comprising at least 25 contiguous amino acids of SEQ ID NOS: 2, 12, 14, or 22;    (b) a polypeptide which is a monocot cyclin D protein;    (c) a polypeptide comprising at least 85% sequence identity to SEQ ID NOS: 2, 12, 14, or 22, wherein the % sequence identity is based on the entire sequence and is determined by GAP 10 using default parameters;    (d) a polypeptide encoded by a nucleic acid of SEQ ID NOS: 1, 11, 13, or 21;    (e) a polypeptide comprising a first domain of the plant CycD specific binding motif LXCXE; a second domain of a WILKV motif; and a third domain of a cyclin box;    (f) a polypeptide encoded by the polynucleotide having the sequence of ATCC deposit having the Designation No. 98848 or 98847; or    (g) a polypeptide characterized by SEQ ID NO: 2, 12, 14, or 22.    
     
     
         14 . A host cell comprising the isolated protein of  claim 13 .  
     
     
         15 . The host cell of  claim 14  that is a plant cell.  
     
     
         16 . The plant cell of  claim 15  that is a transgenic cell.  
     
     
         17 . A ribonucleic acid sequence encoding the isolated protein of  claim 13 .  
     
     
         18 . A method of modulating the level of CycD protein in a cell, comprising: 
 (a) transforming a cell with a recombinant expression cassette comprising a CycD polynucleotide operably linked to a promoter;    (b) growing the cell under cell-growing conditions for a time sufficient to induce expression of the polynucleotide sufficient to modulate CycD protein in the cell.    
     
     
         19 . The method of  claim 18 , wherein the level of CycD protein is modulated upward to provide for positive selection.  
     
     
         20 . A method of increasing transformation efficiency, comprising introducing a CycD polypeptide into a plant cell comprising an exogenous nucleic acid.