Tissue regenerative composition, method of making, and method of use thereof

Abstract

A matrix, including epithelial basement membrane, for inducing repair of mammalian tissue defects and in vitro cell propagation derived from epithelial tissues of a warm-blooded vertebrate.

We claim:  
     
         1 . A devitalized matrix for inducing repair of tissue defects in a mammal, comprising: 
 an isolated devitalized mammalian epithelial basement membrane and tunica propria immediately subjacent to said basement membrane.    
     
     
         2 . The matrix of  claim 1  wherein said basement membrane is derived from urinary bladder.  
     
     
         3 . The matrix of  claim 1  wherein said basement membrane is derived from small intestine.  
     
     
         4 . The matrix of  claim 1  wherein said matrix is sutured at said tissue defect.  
     
     
         5 . The matrix of  claim 1  wherein said matrix is injected into said tissue defect.  
     
     
         6 . The matrix of  claim 1  wherein said matrix is applied to a tissue defect fixation device.  
     
     
         7 . The matrix of  claim 1  wherein said matrix is mixed with a pharmaceutical agent.  
     
     
         8 . A method for inducing repair of a tissue defect in a mammal, said method comprising: 
 providing to a defect site, a devitalized matrix comprising a mammalian epithelial basement membrane and tunica propria immediately subjacent to said basement membrane.    
     
     
         9 . The method of  claim 8  wherein said repair comprises induction of connective tissue repair and epithelial tissue repair at said tissue defect site.  
     
     
         10 . A devitalized composition, comprising: 
 mammalian epithelial basement membrane and tunica propria immediately subjacent to said epithelial basement membrane, wherein said basement membrane and tunica propria are delaminated from cells of an epithelium and abluminal portions of a tunica propria of a mammalian epithelial tissue.    
     
     
         11 . A devitalized composition, comprising: 
 mammalian epithelial basement membrane, tunica propria, and smooth muscle cells of the tunica muscularis, wherein said basement membrane, tunica propria, and smooth muscle are delaminated from epithelial cells of a mammalian epithelium.    
     
     
         12 . A devitalized composition, comprising: 
 mammalian epithelial basement membrane and submucosa, wherein said basement membrane and submucosa are delaminated from the cells of an epithelium and tunica muscularis of a mammalian epithelial tissue.    
     
     
         13 . A method for manufacture of a devitalized tissue graft composition, comprising: 
 soaking a mammalian epithelial tissue in a deepthelializing solution to form a deepithelialized tissue having an epithelial basement membrane; and,    abrading said deepithelialized tissue on an abluminal surface of said tissue to form a delaminated tissue, wherein the delaminated tissue remaining after abrading said tissue, comprises at least a portion of the epithelial basement membrane and said delaminated tissue comprising said basement membrane induces endogenous tissue restoration.    
     
     
         14 . The method of  claim 13  wherein said deepithelializing solution comprises 1.0 N saline.  
     
     
         15 . The method of  claim 13  wherein said abluminal surface comprises a tissue surface deeper than said epithelial basement membrane.  
     
     
         16 . The method of  claim 13  wherein said epithelial tissue comprises urinary bladder.  
     
     
         17 . The method of  claim 13  wherein said epithelial tissue comprises small intestine.