Method for assaying creatine kinase isozyme activity and assay reagent

Abstract

A method for measuring the activity of creatine kinase MB (CK-MB) isozyme which is accurate, high in specificity, convenient, by inhibiting the activity of a mitochondria-localized creatine kinase (mCK) isozyme to avoid the influence of mCK and a measurement reagent therefor are provided. A method for measuring the activity of CK-MB isozyme comprising selectively measuring the enzymatic activity of CK isozymes by an immunological inhibition method, wherein after a treatment of selectively and immunologically eliminating the enzymatic actions of CK-M subunit and mCK is performed, the activity of remaining CK is measured.

1 . A method for measuring the activity of creatine kinase (CK) MB (CK-MB) isozyme comprising selectively measuring the enzymatic activity of CK isozymes by an immunological inhibition method, wherein after a treatment of selectively and immunologically eliminating the enzymatic actions of CK-M subunit and mCK is performed, the activity of remaining CK is measured.  
     
     
         2 . The method for measuring the activity of CK-MB isozyme as claimed in  claim 1 , wherein the treatment of selectively and immunologically eliminating the enzymatic actions of CK-M subunit and mCK is a treatment in which an inhibitory antibody to the CK-M subunit and an inhibitory antibody to the mCK are used.  
     
     
         3 . The method for measuring the activity of CK-MB isozyme as claimed in  claim 2 , wherein the inhibitory antibody to the CK-M subunit and the inhibitory antibody to the mCK are simultaneously acted in one step.  
     
     
         4 . The method for measuring the activity of CK-MB isozyme as claimed in  claim 2 , wherein the inhibitory antibody to the CK-M subunit and the inhibitory antibody to the mCK are acted in different steps.  
     
     
         5 . The method for measuring the activity of CK-MB isozyme as claimed in  claim 2 , wherein the inhibitory antibody to the CK-M subunit is acted and inhibitory antibody to the mCK is acted and the measurement is performed again to simultaneously achieve measurement of the activity of mCK.  
     
     
         6 . A method for measuring the activity of mCK comprising measuring the activity of CK, acting an inhibitory antibody to mCK, and again performing the measurement.  
     
     
         7 . A measurement reagent for measuring CK isozymes, comprising a reagent necessary for a measurement method as claimed in any one of  claims 1  to  6  as a kit or as a single item.