Novel antibody compositions for preparing enriched dendritic cell preparations

Abstract

The present invention relates to antibody composition that are useful in preparing enriched cell preparations such as human hematopoietic progenitor cells and stem cells and non-hematopoietic tumor cells. The invention also relates to kits for carrying out the processes and to the cell preparations prepared by the processes.

We claim:  
     
         54 . A process for enriching and recovering dendritic cells from a sample comprising reacting the sample with an antibody composition containing antibodies capable of binding to the antigens glycophorin A, CD3, CD14, CD16, CD19, CD34, CD56 and CD66b under conditions so that conjugates are formed between the antibodies and the cells in the sample having the antigens glycophorin A, CD3, CD14, CD16, CD19, CD34, CD56 and CD66b on their surfaces; removing the conjugates; and recovering a cell preparation which is enriched in dendritic cells.  
     
     
         56 . A process according to  claim 54  wherein the sample is whole blood.  
     
     
         57 . A process according to  claim 54 , wherein the antibodies in the antibody composition are monoclonal antibodies.  
     
     
         58 . A process according to  claim 54 , wherein the antibodies in the antibody composition are labelled with a marker or they are conjugated to a matrix.  
     
     
         59 . A process according to  claim 54 , wherein the antibodies in the antibody composition are labelled with biotin or a fluorochrome.  
     
     
         60 . A process according to  claim 54 , wherein the matrix is magnetic beads, a panning surface, dense particles for density centrifugation, an adsorption column, or an adsorption membrane.  
     
     
         61 . A process according to  claim 57 , wherein each of the monoclonal antibodies in the antibody composition is incorporated in a tetrameric antibody complex which comprises a first monoclonal antibody of a first animal species from the antibody composition, and a second monoclonal antibody of the first animal species which is capable of binding to at least one antigen on the surface of a matrix, which have been conjugated to form a cyclic tetramer with two monoclonal antibodies of a second animal species directed against the Fc-fragments of the antibodies of the first animal species.  
     
     
         62 . An antibody composition for enriching for dendritic cells comprising antibodies specific for glycophorin A, CD3, CD14, CD16, CD19, CD34, CD56 and CD66b.  
     
     
         63 . An antibody composition according to  claim 62 , wherein the antibodies are monoclonal antibodies.  
     
     
         64 . An antibody composition according to  claim 63  wherein the antibodies are labelled with a marker or they are directly or indirectly conjugated to a matrix.  
     
     
         65 . An antibody composition according to  claim 63  wherein the antibodies are labelled with biotin or a fluorochrome.  
     
     
         66 . An antibody composition according to  claim 64  wherein the matrix is magnetic beads, a panning surface, dense particles for density centrifugation, an adsorption column, or an adsorption membrane.  
     
     
         67 . An antibody composition according to  claim 63  wherein the antibodies are joined to a cytotoxic agent.  
     
     
         68 . An antibody composition according to  claim 63  wherein each of the monoclonal antibodies is incorporated in a tetrameric antibody complex which comprises a first monoclonal antibody of a first animal species from the antibody composition, and a second monoclonal antibody of the first animal species which is capable of binding to at least one antigen on the surface of a matrix, which have been conjugated to form a cyclic tetramer with two monoclonal antibodies of a second animal species directed against the Fc-fragments of the antibodies of the first animal species.