Nucleotide sequence of the Mycoplasma genitalium genome, fragments thereof, and uses thereof

Abstract

The present invention provides the nucleotide sequence of the entire genome of Mycoplasma genitalium , SEQ ID NO: 1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies protein encoding fragments of the genome, and identifies, by position relative to two (2) genes known to flank the origin of replication, any regulatory elements which modulate the expression of the protein encoding fragments of the Mycoplasma genitalium genome.

What is claimed is:  
     
         1 . An isolated polynucleotide comprising the nucleotide sequence of any one of the fragments of SEQ ID NO: 1 depicted in Tables 1a, 1c and 2, or a degenerate variant thereof.  
     
     
         2 . An isolated polynucleotide complementary to the polynucleotide of  claim 1 .  
     
     
         3 . The isolated polynucleotide of  claim 1 , wherein said polynucleotide comprises a heterologous nucleic acid sequence.  
     
     
         4 . A method for making a recombinant vector comprising inserting the isolated polynucleotide of  claim 1  into a vector.  
     
     
         5 . A recombinant vector comprising the isolated polynucleotide of  claim 1 .  
     
     
         6 . The recombinant vector of  claim 5 , wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene expression.  
     
     
         7 . A recombinant host cell comprising the isolated polynucleotide of  claim 1 .  
     
     
         8 . The recombinant host cell of  claim 7 , wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene expression.  
     
     
         9 . An isolated polynucleotide comprising a nucleic acid sequence which hybridizes under hybridization conditions comprising hybridization in 5×SSC and 50% formamide at 50° C. and washing in a wash buffer consisting of 0.5× SSC at 65° C., to the complementary strand of any one of the fragments of SEQ ID NO: 1 depicted in Tables 1a, 1c and 2, or a degenerate variant thereof.  
     
     
         10 . An isolated polynucleotide complementary to the polynucleotide of  claim 9 .  
     
     
         11 . The isolated polynucleotide of  claim 9 , wherein said polynucleotide comprises a heterologous nucleic acid sequence.  
     
     
         12 . A recombinant vector comprising the isolated polynucleotide of  claim 9 .  
     
     
         13 . A recombinant host cell comprising the isolated polynucleotide of  claim 9 .  
     
     
         14 . An isolated polynucleotide comprising at least 50 contiguous nucleotides of any one of the fragments of SEQ ID NO: 1 depicted in Tables 1a, 1c and 2, or a degenerate variant thereof.  
     
     
         15 . The isolated polynucleotide of  claim 14 , wherein said polynucleotide comprises at least 100 contiguous nucleotides of any one of the fragments of SEQ ID NO: 1 depicted in Tables 1a, 1c and 2, or a degenerate variant thereof.  
     
     
         16 . An isolated polynucleotide complementary to the polynucleotide of  claim 14 .  
     
     
         17 . The isolated polynucleotide of  claim 14 , wherein said polynucleotide comprises a heterologous nucleic acid sequence.  
     
     
         18 . A recombinant vector comprising the isolated polynucleotide of  claim 14 .  
     
     
         19 . A recombinant host cell comprising the isolated polynucleotide of  claim 14.