US2003175311A1PendingUtilityA1
Recombinant coccidiosis vaccines
Est. expiryJun 3, 2008(expired)· nominal 20-yr term from priority
Inventors:Mary-Helen BingerRichard Anthony ChizzoniteRichard KramerPeter T. LomedicoStephen McandrewWerner Altenburger
A61P 37/00A61P 37/02A61P 33/02C07K 16/20C07K 14/455C12N 2710/24143C12N 15/86A61K 2039/51A61K 39/00C12N 15/11C12P 21/00A61K 39/02
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Claims
Abstract
This invention provides DNA sequences coding for Eimeria surface antigens, recombinant vectors containing such DNA sequences, transformed microorganisms containing such vectors and methods for producing the antigens using the transformed microorganisms. Methods are also provided for protecting poultry against coccidiosis using the Eimeria surface antigens. The surface antigens can be administered for such protection either as purified proteins or in the form of DNA encoding the proteins in a suitable viral vector such as vaccinia virus.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1 . A protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 28 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711.
2 . The protein of claim 1 encoded by the nucleic acid sequence
ATGGCAGACC TCTTCAGCGG ACTCGTGGGC GGCGTCGTCG GCGCTGTTGC TGCAGCAGAT TTGCCTGCGG AGGGCGAGAG GGCCCCCCGC CCCGCCCCCG GCACTGCCTG GACTTGCTGC TGCAGCAAAC TGCAAGAAGG GGCCCGCGAG CTGGAGGGTT TTGTGCAGCA GCTGAGTTTT GTTGCAGGGA AGCTGGCCTG CTGCCTGCGG GTGGGGGCGG AGCAGCTGGC GCGCTGCGCT GCGGAGGGGC GGCTGCCCAG CAGCAGCAGC AGCAGCAGCT GCTGCGCGCT GCTGCAGCTC GAGAAGCAGG ACCTCGAGCA GAGCCTCGAG GCCGGCAAGC AGGGCGCGGA GTGCCTCTTG AGGAGCAGCA AAGTGGCCCT CGAGGCCCTC CTCGAGGGGG CCCGCGTTGC AGCAACGCGG GGTTTGCTGC TGGTCGAGAG CAGCAAAGAC ACGGTGCTGC GCAGCATTCC CCACACCCAG GAGAAGCTGG CTCAGGCCTA CAGTTCTTTC CTGCGGGGCT ACCAGGGGGC AGCAGCGGGG AGGTCTCTGG GCTACGGGGC CCCTGCTGCT GCTTACGGCC AGCAGCAGCA GCCCAGCAGC TACGGGGCGC CCCCCGCCTC CAGCCAGCAG CCCTCCGGCT TCTTCTGGTA G
or by a functional equivalent or partial sequence thereof.
3 . The protein of claim 1 having the amino acid sequence
MetAlaAspLeuPheSerGlyLeuValGlyGlyValValGlyAlaValAlaAlaAlaAsp LeuProAlaGluGlyGluArgAlaProArgProAlaProGlyThrAlaTrpThrCysCys CysSerLysLeuGlnGluGlyAlaArgGluLeuGluGlyPheValGlnGlnLeuSerPhe ValAlaGlyLysLeuAlaCysCysLeuArgValGlyAlaGluGlnLeuAlaArgCysAla AlaGluGlyArgLeuProSerSerSerSerserSerserCysCysAlaLeuLeuGlnLeu GluLysGlnAspLeuGluGlnserLeuGluAlaGlyLysGlnGlyAlaGluCysLeuLeu ArgSerSerLysLeuAlaLeuGluAlaLeuLeuGluGlyAlaArgValAlaAlaThrArg GlyLeuLeuLeuValGluSerSerLysAspThrValLeuArgSerIleProHisThrGln GluLysLeuAlaGlnAlaTyrSerSerPheLeuArgGlyTyrGlnGlyAlaAlaAlaGly ArgSerLeuGlyTyrGlyAlaProAlaAlaAlaTyrGlyGlnGlnGlnGlnProSerSer TyrGlyAlaProProAlaSerSerGlnGlnProSerGlyPhePheTrp
or a functional equivalent or partial sequence thereof.
4 . A DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 28 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711.
5 . The DNA sequence of claim 4 encoding a protein having the amino acid sequence
MetAlaAspLeuPheSerGlyLeuValGlyGlyValValGlyAlaValAlaAlaAlaAsp LeuProAlaGluGlyGluArgAlaProArgProAlaProGlyThrAlaTrpThrCysCys CysSerLysLeuGlnGluGlyAlaArgGluLeuGluGlyPheValGlnGlnLeuSerPhe ValAlaGlyLysLeuAlaCysCysLeuArgValGlyAlaGluGlnLeuAlaArgCysAla AlaGluGlyArgLeuProSerSerSerSerSerSerSerCysCysAlaLeuLeuGlnLeu GluLysGlnAspLeuGluGlnSerLeuGluAlaGlyLysGlnGlyAlaGluCysLeuLeu ArgSerSerLysLeuAlaLeuGluAlaLeuLeuGluGlyAlaArgValAlaAlaThrArg GlyLeuLeuLeuValGluSerSerLysAspThrValLeuArgSerIleProHisThrGln GluLysLeuAlaGlnAlaTyrSerSerPheLeuArgGlyTyrGlnGlyAlaAlaAlaGly ArgSerLeuGlyTyrGlyAlaProAlaAlaAlaTyrGlyGlnGlnGlnGlnProSerSer TyrGlyAlaProProAlaSerSerGlnGlnProSerGlyPhePheTrp
or a functional equivalent or partial sequence thereof.
6 . The DNA sequence of claim 4 comprising all or part of the nucleotide sequence
ATGGCAGACC TCTTCAGCGG ACTCGTGGGC GGCGTCGTCG GCGCTGTTGC TGCAGCAGAT TTGCCTGCGG AGGGCGAGAG GGCCCCCCGC CCCGCCCCCG GCACTGCCTG GACTTGCTGC TGCAGCAAAC TGCAAGAAGG GGCCCGCGAG CTGGAGGGTT TTGTGCAGCA GCTGAGTTTT GTTGCAGGGA AGCTGGCCTG CTGCCTGCGG GTGGGGGCGG AGCAGCTGGC GCGCTGCGCT GCGGAGGGGC GGCTGCCCAG CAGCAGCAGC AGCAGCAGCT GCTGCGCGCT GCTGCAGCTC GAGAAGCAGG ACCTCGAGCA GAGCCTCGAG GCCGGCAAGC AGGGCGCGGA GTGCCTCTTG AGGAGCAGCA AACTGGCCCT CGAGGCCCTC CTCGAGGGGG CCCGCGTTGC AGCAACGCGG GGTTTGCTGC TGGTCGAGAG CAGCAAAGAC ACGGTGCTGC GCAGCATTCC CCACACCCAG GAGAAGCTGG CTCAGGCCTA CAGTTCTTTC CTGCGGGGCT ACCAGGGGGC AGCAGCGGGG AGGTCTCTGG GCTACGGGGC CCCTGCTGCT GCTTACGGCC AGCAGCAGCA GCCCAGCAGC TACGGGGCGC CCCCCGCCTC CAGCCAGCAG CCCTCCGGCT TCTTCTGGTA G.
7 . A recombinant vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 28 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711.
8 . The recombinant vector of claim 7 comprising a DNA sequence encoding a protein having the amino acid sequence
MetAlaAspLeuPheSerGlyLeuValGlyGlyValValGlyAlaValAlaAlaAlaAsp LeuProAlaGluGlyGluArgAlaProArgProAlaProGlyThrAlaTrpThrCysCys CysSerLysLeuGlnGluGlyAlaArgGluLeuGluGlyPheValGlnGlnLeuSerPhe ValAlaGlyLysLeuAlaCysCysLeuArgValGlyAlaGluGlnLeuAlaArgCysAla AlaGluGlyArgLeuProSerSerSerSerSerSerSerCysCysAlaLeuLeuGlnLeu GluLysGlnAspLeuGluGlnSerLeuGluAlaGlyLysGlnGlyAlaGluCysLeuLeu ArgserSerLysLeuAlaLeuGluAlaLeuLeuGluGlyAlaArgValAlaAlaThrArg GlyLeuLeuLeuValGluSerSerLysAspThrValLeuArgSerIleProHisThrGln GluLysLeuAlaGlnAlaTyrSerSerPheLeuArgGlyTyrGlnGlyAlaAlaAlaGly ArgSrLeuGlyTyrGlyAlaProAlaAlaAlaTyrGlyGlnGlnGlnGlnProSerSer TyrGlyAlaProProAlaSerSerGlnGlnProSerGlyPhePheTrp
or a functional equivalent or partial sequence thereof.
9 . The recombinant vector of claim 7 comprising all or part of the nucleotide sequence
ATGGCAGACC TCTTCAGCGG ACTCGTGGGC GGCGTCGTCG GCGCTGTTGC TGCAGCAGAT TTGCCTGCGG AGGGCGAGAG GGCCCCCCGC CCCGCCCCCG GCACTGCCTG GACTTGCTGC TGCAGCAAAC TGCAAGAAGG GGCCCGCGAG CTGGAGGGTT TTGTGCAGCA GCTGAGTTTT GTTGCAGGGA AGCTGGCCTG CTGCCTGCGG GTGGGGGCGG AGCAGCTGGC GCGCTGCGCT GCGGAGGGGC GGCTGCCCAG CAGCAGCAGC AGCAGCAGCT GCTGCGCGCT GCTGCAGCTC GAGAAGCAGG ACCTCGAGCA GAGCCTCGAG GCCGGCAAGC AGGGCGCGGA GTGCCTCTTG AGGAGCAGCA AACTGGCCCT CGAGGCCCTC CTCGAGGGGG CCCGCGTTGC AGCAACGCGG GGTTTGCTGC TGGTCGAGAG CAGCAAAGAC ACGGTGCTGC GCAGCATTCC CCACACCCAG GAGAAGCTGG CTCAGGCCTA CAGTTCTTTC CTGCGGGGCT ACCAGGGGGC AGCAGCGGGG AGGTCTCTGG GCTACGGGGC CCCTGCTGCT GCTTACGGCC AGCAGCAGCA GCCCAGCAGC TACGGGGCGC CCCCCGCCTC CAGCCAGCAG CCCTCCGGCT TCTTCTGGTA G.
10 . The recombinant vector of claim 7 which recombinant vector is an E. coli vector.
11 . The recombinant vector of claim 7 which is a pox virus vector.
12 . A microorganism transformed with a recombinant vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 28 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711.
13 . A method for producing a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 28 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711, which process comprises:
(a) culturing a microorganism transformed with a recombinant vector comprising a DNA sequence encoding the said protein in a culture medium under conditions in which the DNA sequence is expressed; (b) isolating the said protein from the culture.
14 . A vaccine for protecting poultry against coccidiosis comprising a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 28 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711 and a physiologically acceptable carrier.
15 . A vaccine for protecting poultry against coccidiosis containing a recombinant poxvirus vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 28 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711 and a physiologically acceptable carrier.
16 . A method for protecting poultry against coccidiosis, which method comprises administering an effective amount of a vaccine comprising a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 28 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711 and a physiologically acceptable carrier to a young fowl that is susceptible to coccidiosis.
17 . A method for protecting poultry against coccidiosis, which method comprises administering an effective amount of a vaccine containing a recombinant poxvirus vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 28 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711 and a physiologically acceptable carrier to a young fowl that is susceptible to coccidiosis.
18 . An isolated antibody which is directed against an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 28 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711.
19 . The antibody of claim 18 which is a monoclonal antibody, such as the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9711.
20 . A protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 120 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707.
21 . The protein of claim 20 encoded by the nucleic acid sequence
GAATTCATGT TTAGGCGGAT TTTGTTCCAT GCAAGAAAGC CCACCACCGG CTGCAGGTGG ACTGTACGGG GGACAGACTT TGGAACAACA AGGCATTGCT GTGAGGGAAA CTGCTTCGTG CAGCGAGAAC CCGTGCCCTA TCGACGCAAC GTGCGGAGAA TGGACAGAGT ACAGTGCGTG CTCCAGAACT TGCGGAGGCG GTACCCAAGA GAGGAAGAGG GAGCCGTGGT TGGATAATGC GCAACACGGG GGGCGCACCT GCATGGAACA GTATCCTGAT GGGCCCATAT CGGTCAGGGA GTGCAACACC CAGCCGTGCC CTGTGGACGA AGTAGTTGGT GATTGGGAAG ACTGGGGGCA ATGCAGCGAA CAGTGTGGTG GCGGCAAGCG GACTCGTAAT CGCGGCCCAA GCAAGCAAGA GGCCATGTTC GGAGGCAAGA CAGTTGCTCA ACAGAACGCA GAGCTCCCTG AAGGCGAGAA GATTGAGGTG GTTCAGGAAG AAGGATGCAA TGAAGTTCCA TGCGGACCTT GCACGCTCCC CTTCAGTGAG TGGACCGAAT GCGAGTCGTG CTCCGGGCAT AGAACCAGGG AATCCGCAGT AGCATTTGAT TACACTGACA GAATGTGCAG TGGTGACACA CACGAGGTAC AAAGCTGTGA GGAATACTGT TCCCAAAATG CTGGAGGGGG TGCTGGAGGA GATGGGGGCG CAGGAGGAGG GACTGGAGGC TCTGGAGAGG AGGAAGGAAA GGAGGAATCG AGTGGATTTC CAACTGCAGC TGTAGCCGGT GGCGTGGCTG GGGGAGTCCT CGCGATTGCT GCGGGAGCTG GAGCGTTTTA TGGATTGAGT GGTGGGAGCG CGGCTGCTGC CACTGAAGCA GGTGCTGAAG TGATGACAGA AGCTGGTACA TCCAATGCTG CTGAGGTAGA AAAGGAGAGC CTCATCAGTG CAGGTGAACA ATCAGAGATG TGGGCATCCT AAATGGAAAC GTCGCCGCCG CGGGTTTCGA AAAGGTGCGG ATCTTGCATA TCTGTGAACG AATTATTTAC TAACATCGAG CTCCTTGACC TCCCGTTGGC AAATCATTTA CCAAGCATCT CTGGCGCATA GCTTCTTGAA CAAGACAACG GAATGTCCAA CTGGGGAACA GCTATATTGC GAAGTGTGGT GTTCAAACCA GAAGAGAGCA CAGCGTCATG TGTATGTTAG GGTTGGGCGC CTCCTTTCCC TTATTTATCC CATTTCCTCC GCCTTCATCT TTCCGCCTTC TCTCTGTGCG CCGTATTTTG GGTGTTATTG GTGCCTGGCG GACAGTAAAG AGAGATTGGC GTTATTTGCA GCGTGCGCAG GCCATGGTAG GGTTGGATAA CACTCATTGG TGAAGCGCAA GCCAACAGGG CCACCTTTAC CTCCTGGTGG TCAATGGGGC AGCTTGCTTC TGATCATTGG TTGGTTCTGT TTCAAGGGGC CGGTAATGGG CAGCAGAAGC TTCTGCCAGC CACCACACAA TCGAAGCAAC AAATAAGGGA GGTTCTGCTA ACAATTGTGC GTAGTCATGA TTGTAGGTAG GCTCCGTTTC GAAGATGAAT GACCGGGAGC AGCCTGAATG AAACTTGACT CTCAAAGAAG GGAATTC
or by a functional equivalent or partial sequence thereof.
22 . The protein of claim 20 encoded by the nucleic acid sequence
CTGTACGGG GGACAGACTT TGGAACAACA AGGCATTGCT GTGAGGGAAA CTGCTTCGTG CAGCGAGAAC CCGTGCCCTA TCGACGCAAC GTGCGGAGAA TGGACAGAGT ACAGTGCGTG CTCCAGAACT TGCGGAGGCG GTACCCAAGA GAGGAAGAGG GAGCCGTGGT TGGATAATGC GCAACACGGG GGGCGCACCT GCATGGAACA GTATCCTGAT GGGCCCATAT CGGTCAGGGA GTGCAACACC CAGCCGTGCC CTGTGGACGA AGTAGTTGGT GATTGGGAAG ACTGGGGGCA ATGCAGCGAA CAGTGTGGTG GCGGCAAGCG GACTCGTAAT CGCGGCCCAA GCAAGCAAGA GGCCATGTTC GGAGGCAAGA CAGTTGCTCA ACAGAACGCA GAGCTCCCTG AAGGCGAGAA GATTGAGGTG GTTCAGGAAG AAGGATGCAA TGAAGTTCCA TGCGGACCTT GCACGCTCCC CTTCAGTGAG TGGACCGAAT GCGAGTCGTG CTCCGGGCAT AGAACCAGGG AATCCGCAGT AGCATTTGAT TACACTGACA GAATGTGCAG TGGTGACACA CACGAGGTAC AAAGCTGTGA GGAATACTGT TCCCAAAATG CTGGAGGGGG TGCTGGAGGA GATGGGGGCG CAGGAGGAGG GACTGGAGGC TCTGGAGAGG AGGAAGGAAA GGAGGAATCG AGTGGATTTC CAACTGCAGC TGTAGCCGGT GGCGTGGCTG GGGGAGTCCT CGCCATTGCT GCGGGAGCTG GAGCGTTTTA TGGATTGAGT GGTGGGAGCG CGGCTGCTGC CACTGAAGCA GGTGCTGAAG TGATGACAGA AGCTGGTACA TCCAATGCTG CTGAGGTAGA AAAGGAGAGC CTCATCAGTG CAGGTGAACA ATCAGAGATG TGGGCATCCT AA
or by a functional equivalent or partial sequence thereof.
23 . The protein of claim 20 having the amino acid sequence
SerCysLeuGlyGlyPheCysSerMetGlnGluSerProProProAlaAlaGlyGlyLeu TyrGlyGlyGlnThrLeuGluGlnGlnGlyIleAlaValArgGluThrAlaSerCysSer GluAsnProCysProlleAspAlaThrCysGlyGluTrpThrGluTyrSerAlaCysSer ArgThrCysGlyGlyGlyThrGlnGluArgLysArgGluProTrpLeuAspAsnAlaGln HisGlyGlyArgThrCysMetGluGlnTyrProAspGlyProIleSerValArgGluCys AsnThrGlnProCysProValAspGluValValGlyAspTrpGluAspTrpGlyGlnCys SerGluGlnCysGlyGlyGlyLysArgThrArgAsnArgGlyProSerLysGlnGluAla MetPheGlyGlyLysThrValAlaGlnGlnAsnAlaGluLeuProGluGlyGluLysIle GluValValGlnGluGluGlyCysAsnGluValProCysGlyProCysThrLeuProPhe SerGluTrpThrGluCysGluSerCysSerGlyHisArgThrArgGluSerAlaValAla PheAspTyrThrAspArgMetCysSerGlyAspThrHisGluValGlnSerCysGluGlu TyrCysSerGlnAsnAlaGlyGlyGlyAlaGlyGlyAspGlyGlyAlaGlyGlyGlyThr GlyGlySerGlyGluGluGluGlyLysGluGluSerSerGlyPheProThrAlaAlaVal AlaGlyGlyValAlaGlyGlyValLeuAlaIleAlaAlaGlyAlaGlyAlaPheTyrGly LeuSerGlyGlySerAlaAlaAlaAlaThrGluAlaGlyAlaGluValMetThrGluAla GlyThrSerAsnAlaAlaGluValGluLysGluSerLeuIleSerAlaGlyGluGlnSer GluMetTrpAlaSer
or a functional equivalent or partial sequence thereof.
24 . The protein of claim 20 having the amino acid sequence
LeuTyrGlyGlyGlnThrLeuGluGlnGlnGlyIleAlaValArgGluThrAlaSerCys SerGluAsnProCysProIleAspAlaThrCysGlyGluTrpThrGluTyrSerAlaCys SerArgThrCysGlyGlyGlyThrGlnGluArgLysArgGluProTrpLeuAspAsnAla GlnHisGlyGlyArgThrCysMetGluGlnTyrProAspGlyProIleSerValArgGlu CysAsnThrGlnProCysProValAspGluValValGlyAspTrpGluAspTrpGlyGln CysSerGluGlnCysGlyGlyGlyLysArgThrArgAsnArgGlyProSerLysGlnGlu AlaMetPheGlyGlyLysThrValAlaGlnGlnAsnAlaGluLeuProGluGlyGluLys IleGluValValGlnGluGluGlyCysAsnGluValProCysGlyProCysThrLeuPro PheSerGluTrpThrGluCysGluSerCysSerGlyHisArgThrArgGluSerAlaVal AlaPheAspTyrThrAspArgMetCysSerGlyAspThrHisGluValGlnSerCysGlu GluTyrCysSerGlnAsnAlaGlyGlyGlyAlaGlyGlyAspGlyGlyAlaGlyGlyGly ThrGlyGlySerGlyGluGluGluGlyLysGluGluSerSerGlyPheProThrAlaAla ValAlaGlyGlyValAlaGlyGlyValLeuAlaIleAlaAlaGlyAlaGlyAlaPheTyr GlyLeuSerGlyGlySerAlaAlaAlaAlaThrGluAlaGlyAlaGluValMetThrGlu AlaGlyThrSerAsnAlaAlaGluValGluLysGluSerLeuIleSerAlaGlyGluGln SerGluMetTrpAlaSer
or a functional equivalent or partial sequence thereof.
25 . A DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 120 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707.
26 . The DNA sequence of claim 25 encoding a protein having the amino acid sequence
SerCysLeuGlyGlyPheCysSerMetGlnGluSerProProProAlaAlaGlyGlyLeu TyrGlyGlyGlnThrLeuGluGlnGlnGlyIleAlaValArgGluThrAlaSerCysSer GluAsnProCysProIleAspAlaThrCysGlyGluTrpThrGluTyrSerAlaCysSer ArgThrCysGlyGlyGlyThrGlnGluArgLysArgGluProTrpLeuAspAsnAlaGln HisGlyGlyArgThrCysMetGluGlnTyrProAspGlyProIleSerValArgGluCys AsnThrGlnProCysProValAspGluValValGlyAspTrpGluAspTrpGlyGlnCys SerGluGlnCysGlyGlyGlyLysArgThrArgAsnArgGlyProSerLysGlnGluAla MetPheGlyGlyLysThrValAlaGlnGlnAsnAlaGluLeuProGluGlyGluLysIle GluValValGlnGluGluGlyCysAsnGluValProCysGlyProCysThrLeuProPhe SerGluTrpThrGluCysGluSerCysSerGlyHisArgThrArgGluSerAlaValAla PheAspTyrThrAspArgMetCysSerGlyAspThrHisGluValGlnSerCysGluGlu TyrCysSerGlnAsnAlaGlyGlyGlyAlaGlyGlyAspGlyGlyAlaGlyGlyGlyThr GlyGlySerGlyGluGluGluGlyLysGluGluSerSerGlyPheProThrAlaAlaVal AlaGlyGlyValAlaGlyGlyValLeuAlaIleAlaAlaGlyAlaGlyAlaPheTyrGly LeuSerGlyGlySerAlaAlaAlaAlaThrGluAlaGlyAlaGluValMetThrGluAla GlyThrSerAsnAlaAlaGluValGluLysGluSerLeuIleSerAlaGlyGluGlnSer GluMetTrpAlaSer
or a functional equivalent or par wal sequence thereof.
27 . The DNA sequence of claim 25 encoding a protein having the amino acid sequence
LeuTyrGlyGlyGlnThrLeuGluGlnGlnGlyIleAlaValArgGluThrAlaSerCys SerGluAsnProCysProIleAspAlaThrCysGlyGluTrpThrGluTyrSerAlaCys SerArgThrCysGlyGlyGlyThrGlnGluArgLysArgGluProTrpLeuAspAsnAla GlnHisGlyGlyArgThrCysMetGluGlnTyrProAspGlyProIleSerValArgGlu CysAsnThrGlnProCysProValAspGluValValGlyAspTrpGluAspTrpGlyGln CysSerGluGlnCysGlyGlyGlyLysArgThrArgAsnArgGlyProSerLysGlnGlu AlaMetPheGlyGlyLysThrValAlaGlnGlnAsnAlaGluLeuProGluGlyGluLys IleGluValValGlnGluGluGlyCysAsnGluValProCysGlyProCysThrLeuPro PheSerGluTrpThrGluCysGluSerCysSerGlyHisArgThrArgGluSerAlaVal AlaPheAspTyrThrAspArgMetCysSerGlyAspThrHisGluValGlnSerCysGlu GluTyrCysSerGlnAsnAlaGlyGlyGlyAlaGlyGlyAspGlyGlyAlaGlyGlyGly ThrGlyGlySerGlyGluGluGluGlyLysGluGluSerSerGlyPheProThrAlaAla ValAlaGlyGlyValAlaGlyGlyValLeuAlaIleAlaAlaGlyAlaGlyAlaPheTyr GlyLeuSerGlyGlySerAlaAlaAlaAlaThrGluAlaGlyAlaGluValMetThrGlu AlaGlyThrSerAsnAlaAlaGluValGluLysGluSerLeuIleSerAlaGlyGluGln SerGluMetTrpAlaSer
or a functional equivalent or partial sequence thereof.
28 . The DNA sequence of claim 25 comprising all or part of the nucleotide sequence
GAATTCATGT TTAGGCGGAT TTTGTTCCAT GCAAGAAAGC CCACCACCGG CTGCAGGTGG ACTGTACGGG GGACAGACTT TGGAACAACA AGGCATTGCT GTGAGGGAAA CTGCTTCGTG CAGCGAGAAC CCGTGCCCTA TCGACGCAAC GTGCGGAGAA TGGACAGAGT ACAGTGCGTG CTCCAGAACT TGCGGAGGCG GTACCCAAGA GAGGAAGAGG GAGCCGTGGT TGGATAATGC GCAACACGGG GGGCGCACCT GCATGGAACA GTATCCTGAT GGGCCCATAT CGGTCAGGGA GTGCAACACC CAGCCGTGCC CTGTGGACGA AGTAGTTGGT GATTGGGAAG ACTGGGGGCA ATGCAGCGAA CAGTGTGGTG GCGGCAAGCG GACTCGTAAT CGCGGCCCAA GCAAGCAAGA GGCCATGTTC GGAGGCAAGA CAGTTGCTCA ACAGAACGCA GAGCTCCCTG AAGGCGAGAA GATTGAGGTG GTTCAGGAAG AAGGATGCAA TGAAGTTCCA TGCGGACCTT GCACGCTCCC CTTCAGTGAG TGGACCGAAT GCGAGTCGTG CTCCGGGCAT AGAACCAGGG AATCCGCAGT AGCATTTGAT TACACTGACA GAATGTGCAG TGGTGACACA CACGAGGTAC AAAGCTGTGA GGAATACTGT TCCCAAAATG CTGGAGGGGG TGCTGGAGGA GATGGGGGCG CAGGAGGAGG GACTGGAGGC TCTGGAGAGG AGGAAGGAAA GGAGGAATCG AGTGGATTTC CAACTGCAGC TGTAGCCGGT GGCGTGGCTG GGGGAGTCCT CGCGATTGCT GCGGGAGCTG GAGCGTTTTA TGGATTGAGT GGTGGGAGCG CGGCTGCTGC CACTGAAGCA GGTGCTGAAG TGATGACAGA AGCTGGTACA TCCAATGCTG CTGAGGTAGA AAAGGAGAGC CTCATCAGTG CAGGTGAACA ATCAGAGATG TGGGCATCCT AAATGGAAAC GTCGCCGCCG CGGGTTTCGA AAAGGTGCGG ATCTTGCATA TCTGTGAACG AATTATTTAC TAACATCGAG CTCCTTGACC TCCCGTTGGC AAATCATTTA CCAAGCATCT CTGGCGCATA GCTTCTTGAA CAAGACAACG GAATGTCCAA CTGGGGAACA GCTATATTGC GAAGTGTGGT GTTCAAACCA GAAGAGAGCA CAGCGTCATG TGTATGTTAG GGTTGGGCGC CTCCTTTCCC TTATTTATCC CATTTCCTCC GCCTTCATCT TTCCGCCTTC TCTCTGTGCG CCGTATTTTG GGTGTTATTG GTGCCTGGCG GACAGTAAAG AGAGATTGGC GTTATTTGCA GCGTGCGCAG GCCATGGTAG GGTTGGATAA CACTCATTGG TGAAGCGCAA GCCAACAGGG CCACCTTTAC CTCCTGGTGG TCAATGGGGC AGCTTGCTTC TGATCATTGG TTGGTTCTGT TTCAAGGGGC CGGTAATGGG CAGCAGAAGC TTCTGCCAGC CACCACACAA TCGAAGCAAC AAATAAGGGA GGTTCTGCTA ACAATTGTGC GTAGTCATGA TTGTAGGTAG GCTCCGTTTC GAAGATGAAT GACCGGGAGC AGCCTGAATG AAACTTGACT CTCAAAGAAG GGAATTC.
29 . The DNA sequence of claim 25 comprising all or part of the nucleotide sequence
CTGTACGGG GGACAGACTT TGGAACAACA AGGCATTGCT GTGAGGGAAA CTGCTTCGTG CAGCGAGAAC CCGTGCCCTA TCGACGCAAC GTGCGGAGAA TGGACAGAGT ACAGTGCGTG CTCCAGAACT TGCGGAGGCG GTACCCAAGA GAGGAAGAGG GAGCCGTGGT TGGATAATGC GCAACACGGG GGGCGCACCT GCATGGAACA GTATCCTGAT GGGCCCATAT CGGTCAGGGA GTGCAACACC CAGCCGTGCC CTGTGGACGA AGTAGTTGGT GATTGGGAAG ACTGGGGGCA ATGCAGCGAA CAGTGTGGTG GCGGCAAGCG GACTCGTAAT CGCGGCCCAA GCAAGCAAGA GGCCATGTTC GGAGGCAAGA CAGTTGCTCA ACAGAACGCA GAGCTCCCTG AAGGCGAGAA GATTGAGGTG GTTCAGGAAG AAGGATGCAA TGAAGTTCCA TGCGGACCTT GCACGCTCCC CTTCAGTGAG TGGACCGAAT GCGAGTCGTG CTCCGGGCAT AGAACCAGGG AATCCGCAGT AGCATTTGAT TACACTGACA GAATGTGCAG TGGTGACACA CACGAGGTAC AAAGCTGTGA GGAATACTGT TCCCAAAATG CTGGAGGGGG TGCTGGAGGA GATGGGGGCG CAGGAGGAGG GACTGGAGGC TCTGGAGAGG AGGAAGGAAA GGAGGAATCG AGTGGATTTC CAACTGCAGC TGTAGCCGGT GGCGTGGCTG GGGGAGTCCT CGCCATTGCT GCGGGAGCTG GAGCGTTTTA TGGATTGAGT GGTGGGAGCG CGGCTGCTGC CACTGAAGCA GGTGCTGAAG TGATGACAGA AGCTGGTACA TCCAATGCTG CTGAGGTAGA AAAGGAGAGC CTCATCAGTG CAGGTGAACA ATCAGAGATG TGGGCATCCT AA.
30 . A recombinant vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigen c determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 120 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707.
31 . The recombinant vector of claim 30 comprising a DNA sequence encoding a protein having the amino acid sequence
SerCysLeuGlyGlyPheCysSerMetGlnGluSerProProProAlaAlaGlyGlyLeu TyrGlyGlyGlnThrLeuGluGlnGlnGlyIleAlaValArgGluThrAlaSerCysSer GluAsnProCysProIleAspAlaThrCysGlyGluTrpThrGluTyrSerAlaCysSer ArgThrCysGlyGlyGlyThrGlnGluArgLysArgGluProTrpLeuAspAsnAlaGln HisGlyGlyArgThrCysMetGluGlnTyrProAspGlyProIleSerValArgGluCys AsnThrGlnProCysProValAspGluValValGlyAspTrpGluAspTrpGlyGlnCys SerGluGlnCysGlyGlyGlyLysArgThrArgAsnArgGlyProSerLysGlnGluAla MetPheGlyGlyLysThrValAlaGlnGlnAsnAlaGluLeuProGluGlyGluLysIle GluValValGlnGluGluGlyCysAsnGluValProCysGlyProCysThrLeuProPhe SerGluTrpThrGluCysGluSerCysSerGlyHisArgThrArgGluSerAlaValAla PheAspTyrThrAspArgMetCysSerGlyAspThrHisGluValGlnSerCysGluGlu TyrCysSerGlnAsnAlaGlyGlyGlyAlaGlyGlyAspGlyGlyAlaGlyGlyGlyThr GlyGlySerGlyGluGluGluGlyLysGluGluSerSerGlyPheProThrAlaAlaVal AlaGlyGlyValAlaGlyGlyValLeuAlaIleAlaAlaGlyAlaGlyAlaPheTyrGly LeuSerGlyGlySerAlaAlaAlaAlaThrGluAlaGlyAlaGluValMetThrGluAla GlyThrSerAsnAlaAlaGluValGluLysGluSerLeuIleSerAlaGlyGluGlnSer GluMetTrpAlaSer
or a functional equivalent or partial sequence thereof.
32 . The recombinant vector of claim 30 comprising a DNA sequence encoding a protein having the amino acid sequence
LeuTyrGlyGlyGlnThrLeuGluGlnGlnGlyIleAlaValArgGluThrAlaSerCys SerGluAsnProCysProIleAspAlaThrCysGlyGluTrpThrGluTyrSerAlaCys SerArgThrCysGlyGlyGlyThrGlnGluArgLysArgGluProTrpLeuAspAsnAla GlnHisGlyGlyArgThrCysMetGluGlnTyrProAspGlyProIleSerValArgGlu CysAsnThrGlnProCysProValAspGluValValGlyAspTrpGluAspTrpGlyGln CysSerGluGlnCysGlyGlyGlyLysArgThrArgAsnArgGlyProSerLysGlnGlu AlaMetPheGlyGlyLysThrValAlaGlnGlnAsnAlaGluLeuProGluGlyGluLys IleGluValValGlnGluGluGlyCysAsnGluValProCysGlyProCysThrLeuPro PheSerGluTrpThrGluCysGluSerCysSerGlyHisArgThrArgGluSerAlaVal AlaPheAspTyrThrAspArgMetCysSerGlyAspThrHisGluValGlnSerCysGlu GluTyrCysSerGlnAsnAlaGlyGlyGlyAlaGlyGlyAspGlyGlyAlaGlyGlyGly ThrGlyGlySerGlyGluGluGluGlyLysGluGluSerSerGlyPheProThrAlaAla ValAlaGlyGlyValAlaGlyGlyValLeuAlaIleAlaAlaGlyAlaGlyAlaPheTyr GlyLeuSerGlyGlySerAlaAlaAlaAlaThrGluAlaGlyAlaGluValMetThrGlu AlaGlyThrSerAsnAlaAlaGluValGluLysGluSerLeuIleSerAlaGlyGluGln SerGluMetTrpAlaSer
or a functional equivalent or partial sequence thereof.
33 . The recombinant vector of claim 30 comprising all or part of the nucleotide sequence
GAATTCATGT TTAGGCGGAT TTTGTTCCAT GCAAGAAAGC CCACCACCGG CTGCAGGTGG ACTGTACGGG GGACAGACTT TGGAACAACA AGGCATTGCT GTGAGGGAAA CTGCTTCGTG CAGCGAGAAC CCGTGCCCTA TCGACGCAAC GTGCGGAGAA TGGACAGAGT ACAGTGCGTG CTCCAGAACT TGCGGAGGCG GTACCCAAGA GAGGAAGAGG GAGCCGTGGT TGGATAATGC GCAACACGGG GGGCGCACCT GCATGGAACA GTATCCTGAT GGGCCCATAT CGGTCAGGGA GTGCAACACC CAGCCGTGCC CTGTGGACGA AGTAGTTGGT GATTGGGAAG ACTGGGGGCA ATGCAGCGAA CAGTGTGGTG GCGGCAAGCG GACTCGTAAT CGCGGCCCAA GCAAGCAAGA GGCCATGTTC GGAGGCAAGA CAGTTGCTCA ACAGAACGCA GAGCTCCCTG AAGGCGAGAA GATTGAGGTG GTTCAGGAAG AAGGATGCAA TGAAGTTCCA TGCGGACCTT GCACGCTCCC CTTCAGTGAG TGGACCGAAT GCGAGTCGTG CTCCGGGCAT AGAACCAGGG AATCCGCAGT AGCATTTGAT TACACTGACA GAATGTGCAG TGGTGACACA CACGAGGTAC AAAGCTGTGA GGAATACTGT TCCCAAAATG CTGGAGGGGG TGCTGGAGGA GATGGGGGCG CAGGAGGAGG GACTGGAGGC TCTGGAGAGG AGGAAGGAAA GGAGGAATCG AGTGGATTTC CAACTGCAGC TGTAGCCGGT GGCGTGGCTG GGGGAGTCCT CGCGATTGCT GCGGGAGCTG GAGCGTTTTA TGGATTGAGT GGTGGGAGCG CGGCTGCTGC CACTGAAGCA GGTGCTGAAG TGATGACAGA AGCTGGTACA TCCAATGCTG CTGAGGTAGA AAAGGAGAGC CTCATCAGTG CAGGTGAACA ATCAGAGATG TGGGCATCCT AAATGGAAAC GTCGCCGCCG CGGGTTTCGA AAAGGTGCGG ATCTTGCATA TCTGTGAACG AATTATTTAC TAACATCGAG CTCCTTGACC TCCCGTTGGC AAATCATTTA CCAAGCATCT CTGGCGCATA GCTTCTTGAA CAAGACAACG GAATGTCCAA CTGGGGAACA GCTATATTGC GAAGTGTGGT GTTCAAACCA GAAGAGAGCA CAGCGTCATG TGTATGTTAG GGTTGGGCGC CTCCTTTCCC TTATTTATCC CATTTCCTCC GCCTTCATCT TTCCGCCTTC TCTCTGTGCG CCGTATTTTG GGTGTTATTG GTGCCTGGCG GACAGTAAAG AGAGATTGGC GTTATTTGCA GCGTGCGCAG GCCATGGTAG GGTTGGATAA CACTCATTGG TGAAGCGCAA GCCAACAGGG CCACCTTTAC CTCCTGGTGG TCAATGGGGC AGCTTGCTTC TGATCATTGG TTGGTTCTGT TTCAAGGGGC CGGTAATGGG CAGCAGAAGC TTCTGCCAGC CACCACACAA TCGAAGCAAC AAATAAGGGA GGTTCTGCTA ACAATTGTGC GTAGTCATGA TTGTAGGTAG GCTCCGTTTC GAAGATGAAT GACCGGGAGC AGCCTGAATG AAACTTGACT CTCAAAGAAG GGAATTC.
34 . The recombinant vector of claim 30 comprising all or part of the nucleotide sequence
CTGTACGGG GGACAGACTT TGGAACAACA AGGCATTGCT GTGAGGGAAA CTGCTTCGTG CAGCGAGAAC CCGTGCCCTA TCGACGCAAC GTGCGGAGAA TGGACAGAGT ACAGTGCGTG CTCCAGAACT TGCGGAGGCG GTACCCAAGA GAGGAAGAGG GAGCCGTGGT TGGATAATGC GCAACACGGG GGGCGCACCT GCATGGAACA GTATCCTGAT GGGCCCATAT CGGTCAGGGA GTGCAACACC CAGCCGTGCC CTGTGGACGA AGTAGTTGGT GATTGGGAAG ACTGGGGGCA ATGCAGCGAA CAGTGTGGTG GCGGCAAGCG GACTCGTAAT CGCGGCCCAA GCAAGCAAGA GGCCATGTTC GGAGGCAAGA CAGTTGCTCA ACAGAACGCA GAGCTCCCTG AAGGCGAGAA GATTGAGGTG GTTCAGGAAG AAGGATGCAA TGAAGTTCCA TGCGGACCTT GCACGCTCCC CTTCAGTGAG TGGACCGAAT GCGAGTCGTG CTCCGGGCAT AGAACCAGGG AATCCGCAGT AGCATTTGAT TACACTGACA GAATGTGCAG TGGTGACACA CACGAGGTAC AAAGCTGTGA GGAATACTGT TCCCAAAATG CTGGAGGGGG TGCTGGAGGA GATGGGGGCG CAGGAGGAGG GACTGGAGGC TCTGGAGAGG AGGAAGGAAGGAGGAATCG AGTGGATTTC CAACTGCAGC TGTAGCCGGT GGCGTGGCTG GGGGAGTCCT CGCCATTGCT GCGGGAGCTG GAGCGTTTTA TGGATTGAGT GGTGGGAGCG CGGCTGCTGC CACTGAAGCA GGTGCTGAAG TGATGACAGA AGCTGGTACA TCCAATGCTG CTGAGGTAGA AAAGGAGAGC CTCATCAGTG CAGGTGAACA ATCAGAGATG TGGGCATCCT AA.
35 . The recombinant vector of claim 30 which recombinant vector is an E. coli vector such as pEV/2-4.
36 . The recombinant vector of claim 30 which is a pox virus vector.
37 . A microorganism transformed with a recombinant vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 120 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707.
38 . A method for producing a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 120 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707, which process comprises:
(a) culturing a microorganism transformed with a recombinant vector comprising a DNA sequence encoding the said protein in a culture medium under conditions in which the DNA sequence is expressed; (b) isolating the said protein from the culture.
39 . A vaccine for protecting poultry against coccidiosis comprising a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 120 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707 and a physiologically acceptable carrier.
40 . A vaccine for protecting poultry against coccidiosis containing a recombinant poxvirus vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 120 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707 and a physiologically acceptable carrier.
41 . A method for protecting poultry against coccidiosis, which method comprises administering an effective amount of a vaccine comprising a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 120 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707 and a physiologically acceptable carrier to a young fowl that is susceptible to coccidiosis.
42 . A method for protecting poultry against coccidiosis, which method comprises administering an effective amount of a vaccine containing a recombinant poxvirus vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 120 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707 and a physiologically acceptable carrier to a young fowl that is susceptible to coccidiosis.
43 . An isolated antibody which is directed against an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 120 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707.
44 . The antibody of claim 43 which is a monoclonal antibody, such as the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9707.
45 . A protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 37 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710.
46 . The protein of claim 45 encoded by the nucleic acid sequence
GAATTCCCGA CTTCGAGGGA GGCCCCGGGG GCCTCCCCGC CTGCGAAGCG GCGGCGGACG TCGCTGGGGG CCCCTGCAGC AGGGGAGGGC CCCCTGCGGC GGTGGGAGCA GCCTGCTGCT GGGACTGCAG CAGCAATCCG GCAGCAGGCT GGAGGAGCGG GAGCAGCAGC GGCAGCGCGA GCGGCAGCTG CAGCACGTGC GCGCACCCCC GGGCGCGCAG CAGCAGTGCA GGCGCGGCTA AACGCGTGGG TGGCGGAGGG CAACAAACTG CCAGAGTCCG AGAGAAGAAG AAGGATGTTA GAACAATATA TGAACTTGGA AAAAGTCAAA AAGTTGAGGA AAAAACTCGA CGAAGAAGCA GAAGCCAGGG CCAAATACAT CGAAGGCGGA GTTCAAAAAG AACCCCCACT GGGGGCCCCT CAAGGCCGAA AACCCTTTGC TGCCTTTTGC CCAGAGAGAG GCCGACGAGG CCTACAGGCG GTTCGGCAGG GGCGCTCCCT CTGCGGGGCC CCTCAGGGAG AAGATGCTGC AGGCCCGCAG GAAGTAAAGC AGCAGCAGCA GCAGCAGCAG CAGCGGCAGC GGCAGCGGCA GGGGAGGCGG AGGAGGCAGG GAGGCTTTTG TTTCGAATTG TTTAGGGAGA GGGCGGAGGG CTCGCGGGGT GTATGTACAG CGCGCGAACG CGGCGGCTCA TGTTTGGGTG TTGGATTTCG TCTGCAGAAA ACACGAAGCA AATTAAACTG GCAGAAGTTC CATTTTTCCA CTTTAAAATG CCACTTTTGC TCTTTATATT GATTAAAACT AATGCCATGC TGCTGGCATT CATATAAATG CATTTCCGCG CATTTATGTG CATGCATGGC TGGGCGCGGG TGTCTGTACA CCCCAAGAGC CTTGCTGCTC CGCCGGCGCG AATTTATATT TATATTTCAT TTATGTAAAT ATAAAAGCCT TCAAAAACAC AAATGGACAT TAATTTATCA AGAAAAAAGA TTAAGGAATT C
or by a functional equivalent or partial sequence thereof.
47 . The protein of claim 45 encoded by the nucleic acid sequence
GAATTCCCGA CTTCGAGGGA GGCCCCGGGG GCCTCCCCGC CTGCGAAGCG GCGGCGGACG TCGCTGGGGG CCCCTGCAGC AGGGGAGGGC CCCCTGCGGC GGTGGGAGCA GCCTGCTGCT GGGACTGCAG CAGCAATCCG GCAGCAGCTG GAGGAGCGGG AGCAGCAGCG GCAGCGCGAG CAGCAGCTGC AGCACGTGCA GCGCACCCCC GGGCGCGCAG CAGCAGTGCA GGCGCGGCTA AACGCGTGGG TGGCGGAGGG CAACAAACTG CCAGAGTCCG AGAGAAGAAG AAGGATGTTA GAACAATATA TGAACTTGGA AAAAGTCAAA AAGTTGAGGA AAAAACTCGA CGAAGAAGCA GAAGCCAGGG CCAAATACAT CGAAGGCGAG TTCAAAAAGA ACCCCCACTG GGGGCCCCTC AAGGCCGAAA ACCCTTTGCT GCCTTTTGCC CAGAGAGAGG CCGACGAGGC CTACAGGCGG TTCGGCAGGG GCGCTCCCTC TGCGGGGCCC CTCAGGGAGA AGATGCTGCA GGCCCGCAGG AAGTAAAGCA GCAGCAGCAG CAGCAGCAGC AGCGGCAGCG GCAGCGGCAG GGGAGGCGGA GGAGGCAGGG AGGCTTTTGT TTGAATTGT TTAGGGAGAGG GCGGAGGGCT CGCGGGGTGT ATGTACAGCG CGCGAACGCG CGGGCTCATG TTTGGGTGTT GGATTTCGTC TGCAGAAAAC ACGAAGCAAA TTAAACTGGC AGAAGTTCCA TTTTTCCACT TTAAAATGCC ACTTTTGCTC TTTATATTGA TTAAAACTAA TGCCATGCTG CTGGCATTCA TATAAATGCA TTTCCGCGCA TTTATGTGCA TGCATGGCTG GGCGCGGGTG TCTGTACACC CCAAGAGCCT TGCTGCTCCG CCGGCGCGAA TTTATATTTA TATTTCATTT ATGTAAATAT AAAAGCCTTC AAAAACACAA ATGGACATTA ATTTATCAAG AAAAAAGATT AAGGAATTC
or by a functional equivalent or partial sequence thereof.
48 . The protein of claim 45 encoded by the nucleic acid sequence
CGA CTTCGAGGGA GGCCCCGGGG GCCTCCCCGC CTGCGAAGCG GCGGCGGACG TCGCTGGGGG CCCCTGCAGC AGGGGAGGGC CCCCTGCGGC GGTGGGAGCA GCCTGCTGCT GGGACTGCAG CAGCAATCCG GCAGCAGCTG GAGGAGCGGG AGCAGCAGCG GCAGCGCGAG CAGCAGCTGC AGCACGTGCA GCGCACCCCC GGGCGCGCAG CAGCAGTGCA GGCGCGGCTA AACGCGTGGG TGGCGGAGGG CAACAAACTG CCAGAGTCCG AGAGAAGAAG AAGGATGTTA GAACAATATA TGAACTTGGA AAAAGTCAAA AAGTTGAGGA AAAAACTCGA CGAAGAAGCA GAAGCCAGGG CCAAATACAT CGAAGGCGAG TTCAAAAAGA ACCCCCACTG GGGGCCCCTC AAGGCCGAAA ACCCTTTGCT GCCTTTTGCC CAGAGAGAGG CCGACGAGGC CTACAGGCGG TTCGGCAGGG GCGCTCCCTC TGCGGGGCCC CTCAGGGAGA AGATGCTGCA GGCCCGCAGG AAGTAA
or by a functional equivalent or partial sequence thereof.
49 . The protein of claim 45 having the amino acid sequence
GluPheProThrSerArgGluAlaProGlyAlaSerProProAlaLysArgArgArgThr SerLeuGlyAlaProAlaAlaGlyGluGlyProLeuArgArgTrpGluGlnProAlaAla GlyThrAlAlaAlaIleArgGlnGlnLeuGluGluArgGluGlnGlnArgGlnArgGlu GlnGlnLeuGlnHisValArgSerThrProGlyArgAlaAlaAlaValGlnAlaArgLeu AsnAlaTrpValAlaGluGlyAsnLysLeuProGluSerGluArgArgArgArgMetLeu GluGlnTyrMetAsnLeuGluLysValLysLysLeuArgLysLysLeuAspGluGluAla GluAlaArgAlaLysTyrIleGluGlyGluPheLysLysAsnProHisTrpGlyProLeu LysAlaGluAsnProLeuLeuProPheAlaGlnArgGluAlaAspGluAlaTyrArgArg PheGlyArgGlyAlaProSerAlaGlyProLeuArgGluLysMetLeuGlnAlaArgArg Lys
or a functional equivalent or partial sequence thereof.
50 . The protein of claim 45 having the amino acid sequence
ThrSerArgGluAlaProGlyAlaSerProProAlaLysArgArgArgThrSerLeuGly AlaProAlaAlaGlyGluGlyProLeuArgArgTrpGluGlnProAlaAlaGlyThrAla AlaAlaIleArgGlnGlnLeuGluGluArgGluGlnGlnArgGlnArgGluGlnGlnLeu GlnHisValArgSerThrProGlyArgAlaAlaAlaValGlnAlaArgLeuAsnAlaTrp ValAlaGluGlyAsnLysLeuProGluSerGluArgArgArgArgMetLeuGluGlnTyr MetAsnLeuGluLysValLysLysLeuArgLysLysLeuAspGluGluAlaGluAlaArg AlaLysTyrIleGluGlyGluPheLysLysAsnProHisTrpGlyProLeuLysAlaGlu AsnProLeuLeuProPheAlaGlnArgGluAlaAspGluAlaTyrArgArgPheGlyArg GlyAlaProSerAlaGlyProLeuArgGluLysMetLeuGlnAlaArgArgLys
or a functional equivalent or partial sequence thereof.
51 . A DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 37 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710.
52 . The DNA sequence of claim 51 encoding a protein having the amino acid sequence
GluPheProThrSerArgGluAlaProGlyAlaSerProProAlaLysArgArgArgThr SerLeuGlyAlaProAlaAlaGlyGluGlyProLeuArgArgTrpGluGlnProAlaAla GlyThrAlaAlaAlaIleArgGlnGlnLeuGluGluArgGluGlnGlnArgGlnArgGlu GlnGlnLeuGlnHisValArgSerThrProGlyArgAlaAlaAlaValGlnAlaArgLeu AsnAlaTrpValAlaGluGlyAsnLysLeuProGluSerGluArgArgArgArgMetLeu GluGlnTyrMetAsnLeuGluLysValLysLysLeuArgLysLysLeuAspGluGluAla GluAlaArgAlaLysTyrIleGluGlyGluPheLysLysAsnProHisTrpGlyProLeu LysAlaGluAsnProLeuLeuProPheAlaGlnArgGluAlaAspGluAlaTyrArgArg PheGlyArgGlyAlaProSerAlaGlyProLeuArgGluLysMetLeuGlnAlaArgArg Lys
or a functional equivalent or partial sequence thereof.
53 . The DNA sequence of claim 51 encoding a protein having the amino acid sequence
ThrSerArgGluAlaProGlyAlaSerProProAlaLysArgArgArgThrSerLeuGly AlaProAlaAlaGlyGluGlyProLeuArgArgTrpGluGlnProAlaAlaGlyThrAla AlaAlaIleArgGlnGlnLeuGluGluArgGluGlnGlnArgGlnArgGluGlnGlnLeu GlnHisValArgSerThrProGlyArgAlaAlaAlaValGlnAlaArgLeuAsnAlaTrp ValAlaGluGlyAsnLysLeuProGluSerGluArgArgArgArgMetLeuGluGlnTyr MetAsnLeuGluLysValLysLysLeuArgLysLysLeuAspGluGluAlaGluAlaArg AlaLysTyrIleGluGlyGluPheLysLysAsnProHisTrpGlyProLeuLysAlaGlu AsnProLeuLeuProPheAlaGlnArgGluAlaAspGluAlaTyrArgArgPheGlyArg GlyAlaProSerAlaGlyProLeuArgGluLysMetLeuGlnAlaArgArgLys
or a functional equivalent or partial sequence thereof.
54 . The DNA sequence of claim 51 comprising all or part of the nucleotide sequence
GAATTCCCGA CTTCGAGGGA GGCCCCGGGG GCCTCCCCGC CTGCGAAGCG GCGGCGGACG TCGCTGGGGG CCCCTGCAGC AGGGGAGGGC CCCCTGCGGC GGTGGGAGCA GCCTGCTGCT GGGACTGCAG CAGCAATCCG GCAGCAGGCT GGAGGAGCGG GAGCAGCAGC GGCAGCGCGA GCGGCAGCTG CAGCACGTGC GCGCACCCCC GGGCGCGCAG CAGCAGTGCA GGCGCGGCTA AACGCGTGGG TGGCGGAGGG CAACAAACTG CCAGAGTCCG AGAGAAGAAG AAGGATGTTA GAACAATATA TGAACTTGGA AAAAGTCAAA AAGTTGAGGA AAAAACTCGA CGAAGAAGCA GAAGCCAGGG CCAAATACAT CGAAGGCGGA GTTCAAAAAG AACCCCCACT GGGGGCCCCT CAAGGCCGAA AACCCTTTGC TGCCTTTTGC CCAGAGAGAG GCCGACGAGG CCTACAGGCG GTTCGGCAGG GGCGCTCCCT CTGCGGGGCC CCTCAGGGAG AAGATGCTGC AGGCCCGCAG GAAGTAAAGC AGCAGCAGCA GCAGCAGCAG CAGCGGCAGC GGCAGCGGCA GGGGAGGCGG AGGAGGCAGG GAGGCTTTTG TTTCGAATTG TTTAGGGAGA GGGCGGAGGG CTCGCGGGGT GTATGTACAG CGCGCGAACG CGGCGGCTCA TGTTTGGGTG TTGGATTTCG TCTGCAGAAA ACACGAAGCA AATTAAACTG GCAGAAGTTC CATTTTTCCA CTTTAAAATG CCACTTTTGC TCTTTATATT GATTAAAACT AATGCCATGC TGCTGGCATT CATATAAATG CATTTCCGCG CATTTATGTG CATGCATGGC TGGGCGCGGG TGTCTGTACA CCCCAAGAGC CTTGCTGCTC CGCCGGCGCG AATTTATATT TATATTTCAT TTATGTAAAT ATAAAAGCCT TCAAAAACAC AAATGGACAT TAATTTATCA AGAAAAAAGA TTAAGGAATT C.
55 . The DNA sequence of claim 51 comprising all or part of the nucleotide sequence
GAATTCCCGA CTTCGAGGGA GGCCCCGGGG GCCTCCCCGC CTGCGAAGCG GCGGCGGACG TCGCTGGGGG CCCCTGCAGC AGGGGAGGGC CCCCTGCGGC GGTGGGAGCA GCCTGCTGCT GGGACTGCAG CAGCAATCCG GCAGCAGCTG GAGGAGCGGG AGCAGCAGCG GCAGCGCGAG CAGCAGCTGC AGCACGTGCA GCGCACCCCC GGGCGCGCAG CAGCAGTGCA GGCGCGGCTA AACGCGTGGG TGGCGGAGGG CAACAAACTG CCAGAGTCCG AGAGAAGAAG AAGGATGTTA GAACAATATA TGAACTTGGA AAAAGTCAAA AAGTTGAGGA AAAAACTCGA CGAAGAAGCA GAAGCCAGGG CCAAATACAT CGAAGGCGAG TTCAAAAAGA ACCCCCACTG GGGGCCCCTC AAGGCCGAAA ACCCTTTGCT GCCTTTTGCC CAGAGAGAGG CCGACGAGGC CTACAGGCGG TTCGGCAGGG GCGCTCCCTC TGCGGGGCCC CTCAGGGAGA AGATGCTGCA GGCCCGCAGG AAGTAAAGCA GCAGCAGCAG CAGCAGCAGC AGCGGCAGCG GCAGCGGCAG GGGAGGCGGA GGAGGCAGGG AGGCTTTTGT TTGAATTGT TTAGGGAGAGG GCGGAGGGCT CGCGGGGTGT ATGTACAGCG CGCGAACGCG CGGGCTCATG TTTGGGTGTT GGATTTCGTC TGCAGAAAAC ACGAAGCAAA TTAAACTGGC AGAAGTTCCA TTTTTCCACT TTAAAATGCC ACTTTTGCTC TTTATATTGA TTAAAACTAA TGCCATGCTG CTGGCATTCA TATAAATGCA TTTCCGCGCA TTTATGTGCA TGCATGGCTG GGCGCGGGTG TCTGTACACC CCAAGAGCCT TGCTGCTCCG CCGGCGCGAA TTTATATTTA TATTTCATTT ATGTAAATAT AAAAGCCTTC AAAAACACAA ATGGACATTA ATTTATCAAG AAAAAAGATT AAGGAATTC.
56 . The DNA sequence of claim 51 comprising all or part of the nucleotide sequence
CGA CTTCGAGGGA GGCCCCGGGG GCCTCCCCGC CTGCGAAGCG GCGGCGGACG TCGCTGGGGG CCCCTGCAGC AGGGGAGGGC CCCCTGCGGC GGTGGGAGCA GCCTGCTGCT GGGACTGCAG CAGCAATCCG GCAGCAGCTG GAGGAGCGGG AGCAGCAGCG GCAGCGCGAG CAGCAGCTGC AGCACGTGCA GCGCACCCCC GGGCGCGCAG CAGCAGTGCA GGCGCGGCTA AACGCGTGGG TGGCGGAGGG CAACAAACTG CCAGAGTCCG AGAGAAGAAG AAGGATGTTA GAACAATATA TGAACTTGGA AAAAGTCAAA AAGTTGAGGA AAAAACTCGA CGAAGAAGCA GAAGCCAGGG CCAAATACAT CGAAGGCGAG TTCAAAAAGA ACCCCCACTG GGGGCCCCTC AAGGCCGAAA ACCCTTTGCT GCCTTTTGCC CAGAGAGAGG CCGACGAGGC CTACAGGCGG TTCGGCAGGG GCGCTCCCTC TGCGGGGCCC CTCAGGGAGA AGATGCTGCA GGCCCGCAGG AAGTAA.
57 . A recombinant vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 37 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710.
58 . The recombinant vector of claim 57 comprising a DNA sequence encoding a protein having the amino acid sequence
GluPheProThrSerArgGluAlaProGlyAlaSerProProAlaLysArgArgArgThr SerLeuGlyAlaProAlaAlaGlyGluGlyProLeuArgArgTrpGluGlnProAlaAla GlyThrAlaAlaAlaIleArgGlnGlnLeuGluGluArgGluGlnGlnArgGlnArgGlu GlnGlnLeuGlnHisValArgSerThrProGlyArgAlaAlaAlaValGlnAlaArgLeu AsnAlaTrpValAlaGluGlyAsnLysLeuProGluSerGluArgArgArgArgMetLeu GluGlnTyrMetAsnLeuGluLysValLysLysLeuArgLysLysLeuAspGluGluAla GluAlaArgAlaLysTyrIleGluGlyGluPheLysLysAsnProHisTrpGlyProLeu LysAlaGluAsnProLeuLeuProPheAlaGlnArgGluAlaAspGluAlaTyrArgArg PheGlyArgGlyAlaProSerAlaGlyProLeuArgGluLysMetLeuGlnA,aArgArg Lys
or a functional equivalent or partial sequence thereof.
59 . The recombinant vector of claim 57 comprising a DNA sequence encoding a protein having the amino acid sequence
ThrSerArgGluAlaProGlyAlaSerProProAlaLysArgArgArgThrSerLeuGly AlaProAlaAlaGlyGluGlyProLeuArgArgTrpGluGlnProAlaAlaGlyThrAla AlaAlaIleArgGlnGlnLeuGluGluArgGluGlnGlnArgGlnArgGluGlnGlnLeu GlnHisValArgserThrproGlyArgAlaAlaAlaValGlnAlaArgLeuAsnAlaTrp ValAlaGluGlyAsnLysLeuProGluSerGluArgArgArgArgMetLeuGluGlnTyr MetAsnLeuGluLysValLysLysLeuArgLysLysLeuAspGluGluAlaGluAlaArg AlaLysTyrIleGluGlyGluPheLysLysAsnProHisTrpGlyProLeuLysAlaGlu AsnProLeuLeuProPheAlaGlnArgGluAlaAspGluAlaTyrArgArgPheGlyArg GlyAlaProSerAlaGlyProLeuArgGluLysMetLeuGlnAlaArgArgLys
or a functional equivalent or partial sequence thereof.
60 . The recombinant vector of claim 57 comprising all or part of the nucleotide sequence
GAATTCCCGA CTTCGAGGGA GGCCCCGGGG GCCTCCCCGC CTGCGAAGCG GCGGCGGACG TCGCTGGGGG CCCCTGCAGC AGGGGAGGGC CCCCTGCGGC GGTGGGAGCA GCCTGCTGCT GGGACTGCAG CAGCAATCCG GCAGCAGGCT GGAGGAGCGG GAGCAGCAGC GGCAGCGCGA GCGGCAGCTG CAGCACGTGC GCGCACCCCC GGGCGCGCAG CAGCAGTGCA GGCGCGGCTA AACGCGTGGG TGGCGGAGGG CAACAAACTG CCAGAGTCCG AGAGAAGAAG AAGGATGTTA GAACAATATA TGAACTTGGA AAAAGTCAAA AAGTTGAGGA AAAAACTCGA CGAAGAAGCA GAAGCCAGGG CCAAATACAT CGAAGGCGGA GTTCAAAAAG AACCCCCACT GGGGGCCCCT CAAGGCCGAA AACCCTTTGC TGCCTTTTGC CCAGAGAGAG GCCGACGAGG CCTACAGGCG GTTCGGCAGG GGCGCTCCCT CTGCGGGGCC CCTCAGGGAG AAGATGCTGC AGGCCCGCAG GAAGTAAAGC AGCAGCAGCA GCAGCAGCAG CAGCGGCAGC GGCAGCGGCA GGGGAGGCGG AGGAGGCAGG GAGGCTTTTG TTTCGAATTG TTTAGGGAGA GGGCGGAGGG CTCGCGGGGT GTATGTACAG CGCGCGAACG CGGCGGCTCA TGTTTGGGTG TTGGATTTCG TCTGCAGAAA ACACGAAGCA AATTAAACTG GCAGAAGTTC CATTTTTCCA CTTTAAAATG CCACTTTTGC TCTTTATATT GATTAAAACT AATGCCATGC TGCTGGCATT CATATAAATG CATTTCCGCG CATTTATGTG CATGCATGGC TGGGCGCGGG TGTCTGTACA CCCCAAGAGC CTTGCTGCTC CGCCGGCGCG AATTTATATT TATATTTCAT TTATGTAAAT ATAAAAGCCT TCAAAAACAC AAATGGACAT TAATTTATCA AGAAAAAAGA TTAAGGAATT C.
61 . The recombinant vector of claim 57 comprising all or part of the nucleotide sequence
GAATTCCCGA CTTCGAGGGA GGCCCCGGGG GCCTCCCCGC CTGCGAAGCG GCGGCGGACG TCGCTGGGGG CCCCTGCAGC AGGGGAGGGC CCCCTGCGGC GGTGGGAGCA GCCTGCTGCT GGGACTGCAG CAGCAATCCG GCAGCAGCTG GAGGAGCGGG AGCAGCAGCG GCAGCGCGAG CAGCAGCTGC AGCACGTGCA GCGCACCCCC GGGCGCGCAG CAGCAGTGCA GGCGCGGCTA AACGCGTGGG TGGCGGAGGG CAACAAACTG CCAGAGTCCG AGAGAAGAAG AAGGATGTTA GAACAATATA TGAACTTGGA AAAAGTCAAA AAGTTGAGGA AAAAACTCGA CGAAGAAGCA GAAGCCAGGG CCAAATACAT CGAAGGCGAG TTCAAAAAGA ACCCCCACTG GGGGCCCCTC AAGGCCGAAA ACCCTTTGCT GCCTTTTGCC CAGAGAGAGG CCGACGAGGC CTACAGGCGG TTCGGCAGGG GCGCTCCCTC TGCGGGGCCC CTCAGGGAGA AGATGCTGCA GGCCCGCAGG AAGTAAAGCA GCAGCAGCAG CAGCAGCAGC AGCGGCAGCG GCAGCGGCAG GGGAGGCGGA GGAGGCAGGG AGGCTTTTGT TTGAATTGT TTAGGGAGAGG GCGGAGGGCT CGCGGGGTGT ATGTACAGCG CGCGAACGCG CGGGCTCATG TTTGGGTGTT GGATTTCGTC TGCAGAAAAC ACGAAGCAAA TTAAACTGGC AGAAGTTCCA TTTTTCCACT TTAAAATGCC ACTTTTGCTC TTTATATTGA TTAAAACTAA TGCCATGCTG CTGGCATTCA TATAAATGCA TTTCCGCGCA TTTATGTGCA TGCATGGCTG GGCGCGGGTG TCTGTACACC CCAAGAGCCT TGCTGCTCCG CCGGCGCGAA TTTATATTTA TATTTCATTT ATGTAAATAT AAAAGCCTTC AAAAACACAA ATGGACATTA ATTTATCAAG AAAAAAGATT AAGGAATTC.
62 . The recombinant vector of claim 57 comprising all or part of the nucleotide sequence
CGA CTTCGAGGGA GGCCCCGGGG GCCTCCCCGC CTGCGAAGCG GCGGCGGACG TCGCTGGGGG CCCCTGCAGC AGGGGAGGGC CCCCTGCGGC GGTGGGAGCA GCCTGCTGCT GGGACTGCAG CAGCAATCCG GCAGCAGCTG GAGGAGCGGG AGCAGCAGCG GCAGCGCGAG CAGCAGCTGC AGCACGTGCA GCGCACCCCC GGGCGCGCAG CAGCAGTGCA GGCGCGGCTA AACGCGTGGG TGGCGGAGGG CAACAAACTG CCAGAGTCCG AGAGAAGAAG AAGGATGTTA GAACAATATA TGAACTTGGA AAAAGTCAAA AAGTTGAGGA AAAAACTCGA CGAAGAAGCA GAAGCCAGGG CCAAATACAT CGAAGGCGAG TTCAAAAAGA ACCCCCACTG GGGGCCCCTC AAGGCCGAAA ACCCTTTGCT GCCTTTTGCC CAGAGAGAGG CCGACGAGGC CTACAGGCGG TTCGGCAGGG GCGCTCCCTC TGCGGGGCCC CTCAGGGAGA AGATGCTGCA GGCCCGCAGG AAGTAA.
63 . The recombinant vector of claim 57 which recombinant vector is an E. coli vector.
64 . The recombinant vector of claim 57 which is a pox virus vector.
65 . A microorganism transformed with a recombinant vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 37 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710.
66 . A method for producing a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 37 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710, which process comprises:
(a) culturing a microorganism transformed with a recombinant vector comprising a DNA sequence encoding the said protein in a culture medium under conditions in which the DNA sequence is expressed; (b) isolating the said protein from the culture.
67 . A vaccine for protecting poultry against coccidiosis comprising a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 37 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710 and a physiologically acceptable carrier.
68 . A vaccine for protecting poultry against coccidiosis containing a recombinant poxvirus vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 37 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710 and a physiologically acceptable carrier.
69 . A method for protecting poultry against coccidiosis, which method comprises administering an effective amount of a vaccine comprising a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 37 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710 and a physiologically acceptable carrier to a young-fowl that is susceptible to coccidiosis.
70 . A method for protecting poultry against coccidiosis, which method comprises administering an effective amount of a vaccine containing a recombinant poxvirus vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 37 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710 and a physiologically acceptable carrier to a young fowl that is susceptible to coccidiosis.
71 . An isolated antibody which is directed against an Eimeria surface antigen, which surface antigen has an apparent molecular weight of about 37 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710.
72 . The antibody of claim 71 which is a monoclonal antibody, such as the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9710.
73 . A protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of greater than 200 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712.
74 . The protein of claim 73 encoded by the nucleic acid sequence
GAATTCCGCC CCAGACAGCT AAGCGTGGCA ACATTCTTGG TCTTGTGGGC ATGGTAGCCG CTGTCGTCGT GACCTTCACG GAGGCAGGGT TTGGACAGCA TTACTTGCTG TTTTTCGCCA CTGCTGCACC GGCCCTTGGC CTGGGGCTGT ACATTGCGCA GTCTGTCAAC ATGACTGAGA TGCCTCAACT CGTGGCTCTT TTCCACAGTT TCGTCGGTCT TGCCGCCGTA ATGGTTGGGT TCGCGAACTT CCACTCCCCT GCTGGCGTGG AGCGCGCTTC CTCACTTCTA CGTCTGTTGG AGGTCTACGC CGGCGTTTTC GTCGCCGGTA TCACCTTCAC CGGATCAGTG GTCGCTGCGG CAAAGCTCCA TGGATCGATG GAGAGCCGCT CATTGAGGGT TCCCGGACGC CATGCGTTGA ATACTGCCAC TATTGCTGCC ATTGGCGTAC TTGGCGCTCT TTTTTGCGTC TCTTCTGGCC ACTTTACACG CATGCTTTGC CTTTATGTGA ATGCTGGCTT GAGCATGTGG CTTGGTTTTC ACCTGGTCGC CGCTATTGGT GGAGCTGACA TGCCCGTCGT GATCAGCTTG CTGAACTCGT ATTCCGGAGT GGCGTTGGCT GCCAGTGGCT TCATGTTGGA CAACAACTTG CTGATCATTG CTGGTGCTCT CATCGCGTCA TCTGGTGCCA TTCTGTCTTA CATCATGTGC AAAGGCATGA ACCGGAGTCT GTGGAATGTC GTTCTTGGTG GCTTCGAGGA GGCCGAGGAC GTTGGCGCAG CCAGCCCTCA GGGGGCTGTG CAGCAGGCCA CGGCTGATCA GGTCGCCGAC GAGTTGCTGG CTGCCCGCAA AGTTTTGATC GTGCCTGGAT ACGGAATGGC CGTTGCAAGG TGCCAGAGCG AGCTTGCAGA CATTGCCAAG AACTTGATGA ACTGCGGTAT CACCGTCGAT TTCGGCATCC ATCCAGTTGC TGGTCGCATG CCAGGCCACA TGAACGTCCT CCTCGCTGAG GCTGATGTTC CGTACAAGAT TGTCAAGGAG ATGTCTGAAG TCAACCCGGA AATGAGCTCC TACGACGTCG TCCTGGTTGT TGGAGCCAAC GACACCGTCA ATCCTGCAGC CCTTGAGCCA GGATCAAAGA TCTCAGGAAT GCCCGTTATA GAGGCCTGGA AAGCTAGACG CGTTTTTGTG CTGAAGCGGT CCATGGCTGC TGGATATGCC AGCATTGAAA ATCCACTTTT CCATCTGGAG AACACACGCA TGCTCTTCGG AAACGCAAAG AACACCACTT CTGCAGTCTT CGCCCGTGTC AATGCCAGAG CCGAGCAAAT GCCACCATCT GCTGCCCGTG ATGACCTCGA AGCTGGACTA CTTGAGTTCG ATAGGGAAGA ACGTGTTGAT CCCTCTTCTT GGCCATATCC CAGGATGGCT GTTGGTGTTC TGAGAGACTC CAATGGCTCT GTTATGGTGC CAGTAGCTCC GAAGTTTGTG CCCAAGCTGA GGAAGTTGGC ATTCCGTGTC AATGTCGAGT CTGGTGCTGG CGCCGATGCC GGCTTTACTG ACGAAGAGTA CAGGAGGGCT GGAGCAGAAG TCCTGTCGGG CCCCGATGCA GTCATTAACC AGTCTCAAGT CCTGCTCCGC GTTTCAGCGC CGTCGCCAGA TCTGGTTTCG CGCATTCCTA GGGACAAGGT CCTTATCAGT TACCTATTCC CCAGCATCAA CCAACAAGCT CTTGACATGC TAGCACGCCA AGGCGTCACC GCACTTGCTG TGGATGAGGT TCCTCGCGTC ACAAGAGCAC AGAAGCTAGA CGTGAAGTCT GCTATGCAAG GTCTCCAGGG ATACCGCGCC GTTATCGAAG CGTTCAACGC TCTTCCGAAG CTCAGCAAAG CATCTATCAG TGCTGCAGGC CGTGTAGAAG CCGCTAAAGT TTTCGTCATC GGTGCCGGTG TTGCTGGACT ACAGGCAATT TCTACCGCCC ATGGTTTGGG TGCACAAGTA 8TTGGCCACG ATGTGCGCTC TGCAACTCGT GAGGAAGTCG AATCTTGCGG TGGAAAGTTC ATTGGTTTGA GAATGGGAGA GGAGGGTGAA GTCCTCGGAG GATATGCACG CGAGATGGGT GATGCATATC AGAGAGCGCA ACGCGAGATG ATTGCCAACA CAATCAAGCA CTGCGATGTC GTCATCTGTA CCGCTGCTAT TCACGGCAGA CCTTCACCAA AGCTCATATC ACGCGACATG TTGCGTTCAA TGAAGCCTGG CTCCGTCGTC GTAGATCTTG CAACAGAGTT CGGTGATGTG CGCTCCGGCT GGGGTGGAAA CGTCGAGGTT TCGCCTAAGG ACGACCAGAT TGTCGTTGAT GGCGTCACTG TCATTGGTCG CAGACGCATT GAGACTCGCA TGCCCATTCA GGCGTCTGAG CTGTTCTCCA TGAACATATG CAACCTCCTT GAGGATCTTG GTGGTGGCAG CAACTTCCGC ATCAACATGG ACGACGAAGT CATCAGAGGA TTGGTCGCAG TCTACCAAGG TCGCAACGTG TGGCAGCCAT CGCAGCCCAC TCCTGTTTCC AGGACACCTC CGCGCGGCCA GATGCCGCCC CCGTCTGCAC CTGGTGCACC AGCTCCTGAG AAGCCTGGTG CCTTTGCTCA AGCACTTGCT TCGGATGCAT TCTTCGCAAT GTGTCTTGTT GTTGCTGCCG CTGTTGTCGG GCTCCTTGGC ATTGTCCTTG ACCCTGTGGA GCTCAAGCAT TTGACTCTCC TCGGCTTGTC TCTCATCGTC GGCTACTACT GCGTGTGGGC CGTTACGCCT TCGCTTCACA CACCATTGAT GTCTGTGACG AATGCCCTTT CGGGAGTCAT TGTCATCGGC TGCATGCTCG AGTACGGAAC CGCCATGATA TCCGGATTCA CTCTTCTCGC ACTCATTGGA ACCTTCTTGG CTTCCGTCAA CGTTGCTGGT GGATTCTTCG TAACTCACCG CATGCTGAAG ATGTTTCAGA TATAAGGGGA GAACCCCCTT GAGTTAATCT TAACTCAGAA TAACTCTTTT TCAATTGTAT AAACCTGTAC TCGTTGCAAA AAAAAAAGGA ATTC
or by a functional equivalent or partial sequence thereof.
75 . The protein of claim 73 encoded by the nucleic acid sequence
CC CCAGACAGCT AAGCGTGGCA ACATTCTTGG TCTTGTGGGC ATGGTAGCCG CTGTCGTCGT GACCTTCACG GAGGCAGGGT TTGGACAGCA TTACTTGCTG TTTTTCGCCA CTGCTGCACC GGCCCTTGGC CTGGGGCTGT ACATTGCGCA GTCTGTCAAC ATGACTGAGA TGCCTCAACT CGTGGCTCTT TTCCACAGTT TCGTCGGTCT TGCCGCCGTA ATGGTTGGGT TCGCGAACTT CCACTCCCCT GCTGGCGTGG AGCGCGCTTC CTCACTTCTA CGTCTGTTGG AGGTCTACGC CGGCGTTTTC GTCGCCGGTA TCACCTTCAC CGGATCAGTG GTCGCTGCGG CAAAGCTCCA TGGATCGATG GAGAGCCGCT CATTGAGGGT TCCCGGACGC CATGCGTTGA ATACTGCCAC TATTGCTGCC ATTGGCGTAC TTGGCGCTCT TTTTTGCGTC TCTTCTGGCC ACTTTACACG CATGCTTTGC CTTTATGTGA ATGCTGGCTT GAGCATGTGG CTTGGTTTTC ACCTGGTCGC CGCTATTGGT GGAGCTGACA TGCCCGTCGT GATCAGCTTG CTGAACTCGT ATTCCGGAGT GGCGTTGGCT GCCAGTGGCT TCATGTTGGA CAACAACTTG CTGATCATTG CTGGTGCTCT CATCGCGTCA TCTGGTGCCA TTCTGTCTTA CATCATGTGC AAAGGCATGA ACCGGAGTCT GTGGAATGTC GTTCTTGGTG GCTTCGAGGA GGCCGAGGAC GTTGGCGCAG CCAGCCCTCA GGGGGCTGTG CAGCAGGCCA CGGCTGATCA GGTCGCCGAC GAGTTGCTGG CTGCCCGCAA AGTTTTGATC GTGCCTGGAT ACGGAATGGC CGTTGCAAGG TGCCAGAGCG AGCTTGCAGA CATTGCCAAG AACTTGATGA ACTGCGGTAT CACCGTCGAT TTCGGCATCC ATCCAGTTGC TGGTCGCATG CCAGGCCACA TGAACGTCCT CCTCGCTGAG GCTGATGTTC CGTACAAGAT TGTCAAGGAG ATGTCTGAAG TCAACCCGGA AATGAGCTCC TACGACGTCG TCCTGGTTGT TGGAGCCAAC GACACCGTCA ATCCTGCAGC CCTTGAGCCA GGATCAAAGA TCTCAGGAAT GCCCGTTATA GAGGCCTGGA AAGCTAGACG CGTTTTTGTG CTGAAGCGGT CCATGGCTGC TGGATATGCC AGCATTGAAA ATCCACTTTT CCATCTGGAG AACACACGCA TGCTCTTCGG AAACGCAAAG AACACCACTT CTGCAGTCTT CGCCCGTGTC AATGCCAGAG CCGAGCAAAT GCCACCATCT GCTGCCCGTG ATGACCTCGA AGCTGGACTA CTTGAGTTCG ATAGGGAAGA ACGTGTTGAT CCCTCTTCTT GGCCATATCC CAGGATGGCT GTTGGTGTTC TGAGAGACTC CAATGGCTCT GTTATGGTGC CAGTAGCTCC GAAGTTTGTG CCCAAGCTGA GGAAGTTGGC ATTCCGTGTC AATGTCGAGT CTGGTGCTGG CGCCGATGCC GGCTTTACTG ACGAAGAGTA CAGGAGGGCT GGAGCAGAAG TCCTGTCGGG CCCCGATGCA GTCATTAACC AGTCTCAAGT CCTGCTCCGC GTTTCAGCGC CGTCGCCAGA TCTGGTTTCG CGCATTCCTA GGGACAAGGT CCTTATCAGT TACCTATTCC CCAGCATCAA CCAACAAGCT CTTGACATGC TAGCACGCCA AGGCGTCACC GCACTTGCTG TGGATGAGGT TCCTCGCGTC ACAAGAGCAC AGAAGCTAGA CGTGAAGTCT GCTATGCAAG GTCTCCAGGG ATACCGCGCC GTTATCGAAG CGTTCAACGC TCTTCCGAAG CTCAGCAAAG CATCTATCAG TGCTGCAGGC CGTGTAGAAG CCGCTAAAGT TTTCGTCATC GGTGCCGGTG TTGCTGGACT ACAGGCAATT TCTACCGCCC ATGGTTTGGG TGCACAAGTA TTTGGCCACG ATGTGCGCTC TGCAACTCGT GAGGAAGTCG AATCTTGCGG TGGAAAGTTC ATTGGTTTGA GAATGGGAGA GGAGGGTGAA GTCCTCGGAG GATATGCACG CGAGATGGGT GATGCATATC AGAGAGCGCA ACGCGAGATG ATTGCCAACA CAATCAAGCA CTGCGATGTC GTCATCTGTA CCGCTGCTAT TCACGGCAGA CCTTCACCAA AGCTCATATC ACGCGACATG TTGCGTTCAA TGAAGCCTGG CTCCGTCGTC GTAGATCTTG CAACAGAGTT CGGTGATGTG CGCTCCGGCT GGGGTGGAAA CGTCGAGGTT TCGCCTAAGG ACGACCAGAT TGTCGTTGAT GGCGTCACTG TCATTGGTCG CAGACGCATT GAGACTCGCA TGCCCATTCA GGCGTCTGAG CTGTTCTCCA TGAACATATG CAACCTCCTT GAGGATCTTG GTGGTGGCAG CAACTTCCGC ATCAACATGG ACGACGAAGT CATCAGAGGA TTGGTCGCAG TCTACCAAGG TCGCAACGTG TGGCAGCCAT CGCAGCCCAC TCCTGTTTCC AGGACACCTC CGCGCGGCCA GATGCCGCCC CCGTCTGCAC CTGGTGCACC AGCTCCTGAG AAGCCTGGTG CCTTTGCTCA AGCACTTGCT TCGGATGCAT TCTTCGCAAT GTGTCTTGTT GTTGCTGCCG CTGTTGTCGG GCTCCTTGGC ATTGTCCTTG ACCCTGTGGA GCTCAAGCAT TTGACTCTCC TCGGCTTGTC TCTCATCGTC GGCTACTACT GCGTGTGGGC CGTTACGCCT TCGCTTCACA CACCATTGAT GTCTGTGACG AATGCCCTTT CGGGAGTCAT TGTCATCGGC TGCATGCTCG AGTACGGAAC CGCCATGATA TCCGGATTCA CTCTTCTCGC ACTCATTGGA ACCTTCTTGG CTTCCGTCAA CGTTGCTGGT GGATTCTTCG TAACTCACCG CATGCTGAAG ATGTTTCAGA TATAA
or by a functional equivalent or partial sequence thereof.
76 . The protein of claim 73 having the amino acid sequence
ProGlnThrAlaLysArgGlyAsnIleLeuGlyLeuValGlyMetValAlaAlaValVal ValThrPheThrGluAlaGlyPheGlyGlnHisTyrLeuLeuPhePheAlaThrAlaAla ProAlaLeuGlyLeuGlyLeuTyrIleAlaGlnSerValAsnMetThrGluMetProGln LeuValAlaLeuPheHisSerPheValGlyLeuAlaAlaValMetValGlyPheAlaAsn PheHisSerProAlaGlyValGluArgAlaSerSerLeuLeuArgLeuLeuGluValTyr AlaGlyValPheValAlaGlyIleThrPheThrGlySerValValAlaAlaAlaLysLeu HisGlySerMetGluSerArgSerLeuArgValProGlyArgHisAlaLeuAsnThrAla ThrIleAlaAlaIleGlyValLeuGlyAlaLeuPheCysValSerSerGlyHisPheThr ArgMetLeuCysLeuTyrValAsnAlaGlyLeuSerMetTrpLeuGlyPheHisLeuVal AlaAlaIleGlyGlyAlaAspMetProValValIleSerLeuLeuAsnSerTyrSerGly ValAlaLeuAlaAlaSerGlyPheMetLeuAspAsnAsnLeuLeuIleIleAlaGlyAla LeuIleAlaSerSerGlyAlaIleLeuSerTyrIleMetCysLysGlyMetAsnArgSer LeuTrpAsnValValLeuGlyGlyPheGluGluAlaGluAspValGlyAlaAlaSerPro GlnGlyAlaValGlnGlnAlaThrAlaAspGlnValAlaAspGluLeuLeuAlaAlaArg LysValLeuIleValProGlyTyrGlyMetAlaValAlaArgCysGlnSerGluLeuAla AspIleAlaLysAsnLeuMetAsnCysGlyIleThrValAspPheGlyIleHisProVal AlaGlyArgMetProGlyHisMetAsnValLeuLeuAlaGluAlaAspValProTyrLys IleValLysGluMetSerGluValAsnProGluMetSerSerTyrAspValValLeuVal ValGlyAlaAsnAspThrValAsnProAlaAlaLeuGluProGlySerLysIleSerGly MetProValIleGluAlaTrpLysAlaArgArgValPheValLeuLysArgSerMetAla AlaGlyTyrAlaSerIleGluAsnProLeuPheHisLeuGluAsnThrArgMetLeuPhe GlyAsnAlaLysAsnThrThrSerAlaValPheAlaArgValAsnAlaArgAlaGluGln MetProProSerAlaAlaArgAspAspLeuGluAlaGlyLeuLeuGluPheAspArgGlu GluArgValAspProSerSerTrpProTyrProArgMetAlaValGlyValLeuArgAsp SerAsnGlySerValMetValProValAlaProLysPheValProLysLeuArgLysLeu AlaPheArgValAsnValGluSerGlyAlaGlyAlaAspAlaGlyPheThrAspGluGlu TyrArgArgAlaGlyAlaGluValLeuSerGlyProAspAlaValIleAsnGlnSerGln ValLeuLeuArgValSerAlaProSerProAspLeuValSerArgIleProArgAspLys ValLeuIleSerTyrLeuPheProSerIleAsnGlnGlnAlaLeuAspMetLeuAlaArg GlnGlyValThrAlaLeuAlaValAspGluValProArgValThrArgAlaGlnLysLeu AspValLysSerAlaMetGlnGlyLeuGlnGlyTyrArgAlaValIleGluAlaPheAsn AlaLeuProLysLeuSerLysAlaSerIleSerAlaAlaGlyArgValGluAlaAlaLys ValPheValIleGlyAlaGlyValAlaGlyLeuGlnAlaIleSerThrAlaHisGlyLeu GlyAlaGlnVal???GlyHisAspValArgSerAlaThrArgGluGluValGluSerCys GlyGlyLysPheIleGlyLeuArgMetGlyGluGluGlyGluValLeuGlyGlyTyrAla ArgGluMetGlyAspAlaTyrGlnArgAlaGlnArgGluMetIleAlaAsnThrIleLys HisCysAspValValIleCysThrAlaAlaIleHisGlyArgProSerProLysLeuIle SerArgAspMetLeuArgSerMetLysProGlySerValValValAspLeuAlaThrGlu PheGlyAspValArgSerGlyTrpGlyGlyAsnValGluValSerProLysAspAspGln IleValValAspGlyValThrValIleGlyArgArgArgIleGluThrArgMetProIle GlnAlaSerGluLeuPheSerMetAsnIleCysAsnLeuLeuGluAspLeuGlyGlyGly SerAsnPheArgIleAsnMetAspAspGluValIleArgGlyLeuValAlaValTyrGln GlyArgAsnValTrpGlnProSerGlnProThrProValSerArgThrProProArgGly GlnMetProProProSerAlaProGlyAlaProAlaProGluLysProGlyAlaPheAla GlnAlaLeuAlaSerAspAlaPhePheAlaMetCysLeuValValAlaAlaAlaValVal GlyLeuLeuGlyIleValLeuAspProValGluLeuLysHisLeuThrLeuLeuGlyLeu SerLeuIleValGlyTyrTyrCysValTrpAlaValThrProSerLeuHisThrProLeu MetSerValThrAsnAlaLeuSerGlyValIleValIleGlyCysMetLeuGluTyrGly ThrAlaMetIleSerGlyPheThrLeuLeuAlaLeuIleGlyThrPheLeuAlaSerVal AsnValAlaGlyGlyPhePheValThrHisArgMetLeuLysMetPheGlnIle
or a functional equivalent or partial sequence thereof.
77 . The protein of claim 73 having the amino acid sequence
ThrAlaLysArgGlyAsnIleLeuGlyLeuValGlyMetValAlaAlaValVal ValThrPheThrGluAlaGlyPheGlyGlnHisTyrLeuLeuPhePheAlaThrAlaAla ProAlaLeuGlyLeuGlyLeuTyrIleAlaGlnSerValAsnMetThrGluMetProGln LeuValAlaLeuPheHisSerPheValGlyLeuAlaAlaValMetValGlyPheAlaAsn PheHisSerProAlaGlyValGluArgAlaSerSerLeuLeuArgLeuLeuGluValTyr AlaGlyValPheValAlaGlyIleThrPheThrGlySerValValAlaAlaAlaLysLeu HisGlySerMetGluSerArgSerLeuArgValProGlyArgHisAlaLeuAsnThrAla ThrIleAlaAlaIleGlyValLeuGlyAlaLeuPheCysValSerSerGlyHisPheThr ArgMetLeuCysLeuTyrValAsnAlaGlyLeuSerMetTrpLeuGlyPheHisLeuVal AlaAlaIleGlyGlyAlaAspMetProValValIleSerLeuLeuAsnSerTyrSerGly ValAlaLeuAlaAlaSerGlyPheMetLeuAspAsnAsnLeuLeuIleIleAlaGlyAla LeuIleAlaSerSerGlyAlaIleLeuSerTyrIleMetCysLysGlyMetAsnArgSer LeuTrpAsnValValLeuGlyGlyPheGluGluAlaGluAspValGlyAlaAlaSerPro GlnGlyAlaValGlnGlnAlaThrAlaAspGlnValAlaAspGluLeuLeuAlaAlaArg LysValLeuIleValProGlyTyrGlyMetAlaValAlaArgCysGlnSerGluLeuAla AspIleAlaLysAsnLeuMetAsnCysGlyIleThrValAspPheGlyIleHisProVal AlaGlyArgMetProGlyHisMetAsnValLeuLeuAlaGluAlaAspValProTyrLys IleValLysGluMetSerGluValAsnProGluMetSerSerTyrAspValValLeuVal ValGlyAlaAsnAspThrValAsnProAlaAlaLeuGluProGlySerLysIleSerGly MetProValIleGluAlaTrpLysAlaArgArgValPheValLeuLysArgserMetAla AlaGlyTyrAlaSerIleGluAsnProLeuPheHisLeuGluAsnThrArgMetLeuPhe GlyAsnAlaLysAsnThrThrSerAlaValPheAlaArgValAsnAlaArgAlaGluGln MetProProSerAlaAlaArgAspAspLeuGluAlaGlyLeuLeuGluPheAspArgGlu GluArgValAspProSerSerTrpProTyrProArgMetAlaValGlyValLeuArgAsp SerAsnGlySerValMetValProValAlaProLysPheValProLysLeuArgLysLeu AlaPheArgValAsnValGluSerGlyAlaGlyAlaAspAlaGlyPheThrAspGluGlu TyrArgArgAlaGlyAlaGluValLeuSerGlyProAspAlaValIleAsnGlnSerGln ValLeuLeuArgValSerAlaProSerProAspLeuValSerArgIleProArgAspLys ValLeuIleSerTyrLeuPheProSerIleAsnGlnGlnAlaLeuAspMetLeuAlaArg GlnGlyValThrAlaLeuAlaValAspGluValProArgValThrArgAlaGlnLysLeu AspValLysSerAlaMetGlnGlyLeuGlnGlyTyrArgAlaValIleGluAlaPheAsn AlaLeuProLysLeuSerLysAlaSerIleSerAlaAlaGlyArgValGluAlaAlaLys ValPheValIleGlyAlaGlyValAlaGlyLeuGlnAlaIleSerThrAlaHisGlyLeu GlyAlaGlnValPheGlyHisAspValArgSerAlaThrArgGluGluValGluSerCys GlyGlyLysPheIleGlyLeuArgMetGlyGluGluGlyGluValLeuGlyGlyTyrAla ArgGluMetGlyAspAlaTyrGlnArgAlaGlnArgGluMetIleAlaAsnThrIleLys HisCysAspValValIleCysThrAlaAlaIleHisGlyArgProSerProLysLeuIle SerArgAspMetLeuArgSerMetLysProGlySerValValValAspLeuAlaThrGlu PheGlyAspValArgSerGlyTrpGlyGlyAsnValGluValSerProLysAspAspGln IleValValAspGlyValThrValIleGlyArgArgArgIleGluThrArgMetProIle GlnAlaSerGluLeuPheSerMetAsnIleCysAsnLeuLeuGluAspLeuGlyGlyGly SerAsnPheArgIleAsnMetAspAspGluValIleArgGlyLeuValAlaValTyrGln GlyArgAsnValTrpGlnProSerGlnProThrProValSerArgThrProProArgGly GlnMetProProProSerAlaProGlyAlaProAlaProGluLysProGlyAlaPheAla GlnAlaLeuAlaSerAspAlaPhePheAlaMetCysLeuValValAlaAlaAlaValVal GlyLeuLeuGlyIleValLeuAspProValGluLeuLysHisLeuThrLeuLeuGlyLeu SerLeuIleValGlyTyrTyrCysValTrpAlaValThrProSerLeuHisThrProLeu MetSerValThrAsnAlaLeuSerGlyValIleValIleGlyCysMetLeuGluTyrGly ThrAlaMetIleSerGlyPheThrLeuLeuAlaLeuIleGlyThrPheLeuAlaSerVal AsnValAlaGlyGlyPhePheValThrHisArgMetLeuLysMetPheGlnIle
or a functional equivalent or partial sequence thereof.
78 . A DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of greater than 200 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712.
79 . The DNA sequence of claim 78 encoding a protein having the amino acid sequence
ProGlnThrAlaLysArgGlyAsnIleLeuGlyLeuValGlyMetValAlaAlaValVal ValThrPheThrGluAlaGlyPheGlyGlnHisTyrLeuLeuPhePheAlaThrAlaAla ProAlaLeuGlyLeuGlyLeuTyrIleAlaGlnSerValAsnMetThrGluMetProGln LeuValAlaLeuPheHisSerPheValGlyLeuAlaAlaValMetValGlyPheAlaAsn PheHisSerProAlaGlyValGluArgAlaSerSerLeuLeuArgLeuLeuGluValTyr AlaGlyValPheValAlaGlyIleThrPheThrGlySerValValAlaAlaAlaLysLeu HisGlySerMetGluSerArgSerLeuArgValProGlyArgHisAlaLeuAsnThrAla ThrIleAlaAlaIleGlyValLeuGlyAlaLeuPheCysValSerSerGlyHisPheThr ArgMetLeuCysLeuTyrValAsnAlaGlyLeuSerMetTrpLeuGlyPheHisLeuVal AlaAlaIleGlyGlyAlaAspMetProValValIleSerLeuLeuAsnSerTyrSerGly ValAlaLeuAlaAlaSerGlyPheMetLeuAspAsnAsnLeuLeuIleIleAlaGlyAla LeuIleAlaSerSerGlyAlaIleLeuSerTyrIleMetCysLysGlyMetAsnArgser LeuTrpAsnValValLeuGlyGlyPheGluGluAlaGluAspValGlyAlaAlaSerPro GlnGlyAlaValGlnGlnAlaThrAlaAspGlnValAlaAspGluLeuLeuAlaAlaArg LysValLeuIleValProGlyTyrGlyMetAlaValAlaArgCysGlnSerGluLeuAla AspIleAlaLysAsnLeuMetAsnCysGlyIleThrValAspPheGlyIleHisProVal AlaGlyArgMetProGlyHisMetAsnValLeuLeuAlaGluAlaAspValProTyrLys IleValLysGluMetSerGluValAsnProGluMetSerSerTyrAspValValLeuVal ValGlyAlaAsnAspThrValAsnProAlaAlaLeuGluProGlySerLysIleSerGly MetProValIleGluAlaTrpLysAlaArgArgValPheValLeuLysArgserMetAla AlaGlyTyrAlaSerIleGluAsnProLeuPheHisLeuGluAsnThrArgMetLeuPhe GlyAsnAlaLysAsnThrThrSerAlaValPheAlaArgValAsnAlaArgAlaGluGln MetProProSerAlaAlaArgAspAspLeuGluAlaGlyLeuLeuGluPheAspArgGlu GluArgValAspProSerSerTrpProTyrProArgMetAlaValGlyValLeuArgAsp SerAsnGlySerValMetValProValAlaProLysPheValProLysLeuArgLysLeu AlaPheArgValAsnValGluSerGlyAlaGlyAlaAspAlaGlyPheThrAspGluGlu TyrArgArgAlaGlyAlaGluValLeuSerGlyProAspAlaValIleAsnGlnSerGln ValLeuLeuArgValSerAlaProSerProAspLeuValSerArgIleProArgAspLys ValLeuIleSerTyrLeuPheProSerIleAsnGlnGlnAlaLeuAspMetLeuAlaArg GlnGlyValThrAlaLeuAlaValAspGluValProArgValThrArgAlaGlnLysLeu AspValLysSerAlaMetGlnGlyLeuGlnGlyTyrArgAlaValIleGluAlaPheAsn AlaLeuProLysLeuSerLysAlaSerIleSerAlaAlaGlyArgValGluAlaAlaLys ValPheValIleGlyAlaGlyValAlaGlyLeuGlnAlaIleSerThrAlaHisGlyLeu GlyAlaGlnVal???GlyHisAspValArgSerAlaThrArgGluGluValGluSerCys GlyGlyLysPheIleGlyLeuArgMetGlyGluGluGlyGluValLeuGlyGlyTyrAla ArgGluMetGlyAspAlaTyrGlnArgAlaGlnArgGluMetIleAlaAsnThrIleLys HisCysAspValValIleCysThrAlaAlaIleHisGlyArgProSerProLysLeuIle SerArgAspMetLeuArgSerMetLysProGlySerValValValAspLeuAlaThrGlu PheGlyAspValArgSerGlyTrpGlyGlyAsnValGluValSerProLysAspAspGln IleValValAspGlyValThrValIleGlyArgArgArgIleGluThrArgMetProIle GlnAlaSerGluLeuPheSerMetAsnIleCysAsnLeuLeuGluAspLeuGlyGlyGly SerAsnPheArgIleAsnMetAspAspGluValIleArgGlyLeuValAlaValTyrGln GlyArgAsnValTrpGlnProSerGlnProThrProValSerArgThrProProArgGly GlnMetProProProSerAlaProGlyAlaProAlaProGluLysProGlyAlaPheAla GlnAlaLeuAlaSerAspAlaPhePheAlaMetCysLeuValValAlaAlaAlaValVal GlyLeuLeuGlyIleValLeuAspProValGluLeuLysHisLeuThrLeuLeuGlyLeu SerLeuIleValGlyTyrTyrCysValTrpAlaValThrProSerLeuHisThrProLeu MetSerValThrAsnAlaLeuSerGlyValIleValIleGlyCysMetLeuGluTyrGly ThrAlaMetIleSerGlyPheThrLeuLeuAlaLeuIleGlyThrPheLeuAlaSerVal AsnValAlaGlyGlyphepheValThrHisArgMetLeuLysMetPheGlnIle
or a functional equivalent or partial sequence thereof.
80 . The DNA sequence of claim 78 encoding a protein having the amino acid sequence
ThrAlaLysArgGlyAsnIleLeuGlyLeuValGlyMetValAlaAlaValVal ValThrPheThrGluAlaGlyPheGlyGlnHisTyrLeuLeuPhePheAlaThrAlaAla ProAlaLeuGlyLeuGlyLeuTyrIleAlaGlnSerValAsnMetThrGluMetProGln LeuValAlaLeupheHisSerpheValGlyLeuAlaAlaValMetValGlyPheAlaAsn PheHisSerProAlaGlyValGluArgAlaSerSerLeuLeuArgLeuLeuGluValTyr AlaGlyValPheValAlaGlyIleThrPheThrGlySerValValAlaAlaAlaLysLeu HisGlySerMetGluSerArgSerLeuArgValProGlyArgHisAlaLeuAsnThrAla ThrIleAlaAlaIleGlyValLeuGlyAlaLeuPheCysValSerSerGlyHisPheThr ArgMetLeuCysLeuTyrValAsnAlaGlyLeuSerMetTrpLeuGlyPheHisLeuVal AlaAlaIleGlyGlyAlaAspMetProValValIleSerLeuLeuAsnSerTyrSerGly ValAlaLeuAlaAlaSerGlyPheMetLeuAspAsnAsnLeuLeuIleIleAlaGlyAla LeuIleAlaSerSerGlyAlaIleLeuSerTyrIleMetCysLysGlyMetAsnArgSer LeuTrpAsnValValLeuGlyGlyPheGluGluAlaGluAspValGlyAlaAlaSerPro GlnGlyAlaValGlnGlnAlaThrAlaAspGlnValAlaAspGluLeuLeuAlaAlaArg LysValLeuIleValProGlyTyrGlyMetAlaValAlaArgCysGlnSerGluLeuAla AspIleAlaLysAsnLeuMetAsnCysGlyIleThrValAspPheGlyIleHisProVal AlaGlyArgMetProGlyHisMetAsnValLeuLeuAlaGluAlaAspValProTyrLys IleValLysGluMetSerGluValAsnProGluMetSerSerTyrAspValValLeuVal ValGlyAlaAsnAspThrValAsnProAlaAlaLeuGluProGlySerLysIleSerGly MetProValIleGluAlaTrpLysAlaArgArgValPheValLeuLysArgSerMetAla AlaGlyTyrAlaSerIleGluAsnProLeuPheHisLeuGluAsnThrArgMetLeuPhe GlyAsnAlaLysAsnThrThrSerAlaValPheAlaArgValAsnAlaArgAlaGluGln MetProProSerAlaAlaArgAspAspLeuGluAlaGlyLeuLeuGluPheAspArgGlu GluArgValAspProSerSerTrpProTyrProArgMetAlaValGlyValLeuArgAsp SerAsnGlySerValMetValProValAlaProLysPheValProLysLeuArgLysLeu AlaPheArgValAsnValGluSerGlyAlaGlyAlaAspAlaGlyPheThrAspGluGlu TyrArgArgAlaGlyAlaGluValLeuSerGlyProAspAlaValIleAsnGlnSerGln ValLeuLeuArgValSerAlaProSerProAspLeuValSerArgIleProArgAspLys ValLeuIleSerTyrLeuPheProSerIleAsnGlnGlnAlaLeuAspMetLeuAlaArg GlnGlyValThrAlaLeuAlaValAspGluValProArgValThrArgAlaGlnLysLeu AspValLysSerAlaMetGlnGlyLeuGlnGlyTyrArgAlaValIleGluAlaPheAsn AlaLeuProLysLeuSerLysAlaSerIleSerAlaAlaGlyArgValGluAlaAlaLys ValPheValIleGlyAlaGlyValAlaGlyLeuGlnAlaIleSerThrAlaHisGlyLeu GlyAlaGlnValPheGlyHisAspValArgSerAlaThrArgGluGluValGluSerCys GlyGlyLysPheIleGlyLeuArgMetGlyGluGluGlyGluValLeuGlyGlyTyrAla ArgGluMetGlyAspAlaTyrGlnArgAlaGlnArgGluMetIleAlaAsnThrIleLys HisCysAspValValIleCysThrAlaAlaIleHisGlyArgProSerProLysLeuIle SerArgAspMetLeuArgSerMetLysProGlySerValValwalAspLeuAlaThrGlu PheGlyAspValArgSerGlyTrpGlyGlyAsnValGluValSerPfoLysAspAspGln IleValValAspGlyValThrValIleGlyArgArgArgIleGluThrArgMetProIle GlnAlaSerGluLeuPheSerMetAsnIleCysAsnLeuLeuGluAspLeuGlyGlyGly SerAsnPheArgIleAsnMetAspAspGluValIleArgGlyLeuValAlaValTyrGln GlyArgAsnValTrpGlnProSerGlnProThrProValSerArgThrProProArgGly GlnMetProProProSerAlaProGlyAlaProAlaProGluLysProGlyAlaPheAla GlnAlaLeuAlaSerAspAlaPhePheAlaMetCysLeuValValAlaAlaAlaValVal GlyLeuLeuGlyIleValLeuAspProValGluLeuLysHisLeuThrLeuLeuGlyLeu SerLeuIleValGlyTyrTyrCysValTrpAlaValThrProSerLeuHisThrProLeu MetSerValThrAsnAlaLeuSerGlyVallleVallleGlyCysMetLeuGluTyrGly ThrAlaMetIleSerGlyPheThrLeuLeuAlaLeuIleGlyThrPheLeuAlaSerVal AsnValAlaGlyGlyPhePheValThrHisArgMetLeuLysMetPheGlnIle
or a functional equivalent or partial sequence thereof.
81 . The DNA sequence of claim 78 comprising all or part of the nucleotide sequence
GAATTCCGCC CCAGACAGCT AAGCGTGGCA ACATTCTTGG TCTTGTGGGC ATGGTAGCCG CTGTCGTCGT GACCTTCACG GAGGCAGGGT TTGGACAGCA TTACTTGCTG TTTTTCGCCA CTGCTGCACC GGCCCTTGGC CTGGGGCTGT ACATTGCGCA GTCTGTCAAC ATGACTGAGA TGCCTCAACT CGTGGCTCTT TTCCACAGTT TCGTCGGTCT TGCCGCCGTA ATGGTTGGGT TCGCGAACTT CCACTCCCCT GCTGGCGTGG AGCGCGCTTC CTCACTTCTA CGTCTGTTGG AGGTCTACGC CGGCGTTTTC GTCGCCGGTA TCACCTTCAC CGGATCAGTG GTCGCTGCGG CAAAGCTCCA TGGATCGATG GAGAGCCGCT CATTGAGGGT TCCCGGACGC CATGCGTTGA ATACTGCCAC TATTGCTGCC ATTGGCGTAC TTGGCGCTCT TTTTTGCGTC TCTTCTGGCC ACTTTACACG CATGCTTTGC CTTTATGTGA ATGCTGGCTT GAGCATGTGG CTTGGTTTTC ACCTGGTCGC CGCTATTGGT GGAGCTGACA TGCCCGTCGT GATCAGCTTG CTGAACTCGT ATTCCGGAGT GGCGTTGGCT GCCAGTGGCT TCATGTTGGA CAACAACTTG CTGATCATTG CTGGTGCTCT CATCGCGTCA TCTGGTGCCA TTCTGTCTTA CATCATGTGC AAAGGCATGA ACCGGAGTCT GTGGAATGTC GTTCTTGGTG GCTTCGAGGA GGCCGAGGAC GTTGGCGCAG CCAGCCCTCA GGGGGCTGTG CAGCAGGCCA CGGCTGATCA GGTCGCCGAC GAGTTGCTGG CTGCCCGCAA AGTTTTGATC GTGCCTGGAT ACGGAATGGC CGTTGCAAGG TGCCAGAGCG AGCTTGCAGA CATTGCCAAG AACTTGATGA ACTGCGGTAT CACCGTCGAT TTCGGCATCC ATCCAGTTGC TGGTCGCATG CCAGGCCACA TGAACGTCCT CCTCGCTGAG GCTGATGTTC CGTACAAGAT TGTCAAGGAG ATGTCTGAAG TCAACCCGGA AATGAGCTCC TACGACGTCG TCCTGGTTGT TGGAGCCAAC GACACCGTCA ATCCTGCAGC CCTTGAGCCA GGATCAAAGA TCTCAGGAAT GCCCGTTATA GAGGCCTGGA AAGCTAGACG CGTTTTTGTG CTGAAGCGGT CCATGGCTGC TGGATATGCC AGCATTGAAA ATCCACTTTT CCATCTGGAG AACACACGCA TGCTCTTCGG AAACGCAAAG AACACCACTT CTGCAGTCTT CGCCCGTGTC AATGCCAGAG CCGAGCAAAT GCCACCATCT GCTGCCCGTG ATGACCTCGA AGCTGGACTA CTTGAGTTCG ATAGGGAAGA ACGTGTTGAT CCCTCTTCTT GGCCATATCC CAGGATGGCT GTTGGTGTTC TGAGAGACTC CAATGGCTCT GTTATGGTGC CAGTAGCTCC GAAGTTTGTG CCCAAGCTGA GGAAGTTGGC ATTCCGTGTC AATGTCGAGT CTGGTGCTGG CGCCGATGCC GGCTTTACTG ACGAAGAGTA CAGGAGGGCT GGAGCAGAAG TCCTGTCGGG CCCCGATGCA GTCATTAACC AGTCTCAAGT CCTGCTCCGC GTTTCAGCGC CGTCGCCAGA TCTGGTTTCG CGCATTCCTA GGGACAAGGT CCTTATCAGT TACCTATTCC CCAGCATCAA CCAACAAGCT CTTGACATGC TAGCACGCCA AGGCGTCACC GCACTTGCTG TGGATGAGGT TCCTCGCGTC ACAAGAGCAC AGAAGCTAGA CGTGAAGTCT GCTATGCAAG GTCTCCAGGG ATACCGCGCC GTTATCGAAG CGTTCAACGC TCTTCCGAAG CTCAGCAAAG CATCTATCAG TGCTGCAGGC CGTGTAGAAG CCGCTAAAGT TTTCGTCATC GGTGCCGGTG TTGCTGGACT ACAGGCAATT TCTACCGCCC ATGGTTTGGG TGCACAAGTA 8TTGGCCACG ATGTGCGCTC TGCAACTCGT GAGGAAGTCG AATCTTGCGG TGGAAAGTTC ATTGGTTTGA GAATGGGAGA GGAGGGTGAA GTCCTCGGAG GATATGCACG CGAGATGGGT GATGCATATC AGAGAGCGCA ACGCGAGATG ATTGCCAACA CAATCAAGCA CTGCGATGTC GTCATCTGTA CCGCTGCTAT TCACGGCAGA CCTTCACCAA AGCTCATATC ACGCGACATG TTGCGTTCAA TGAAGCCTGG CTCCGTCGTC GTAGATCTTG CAACAGAGTT CGGTGATGTG CGCTCCGGCT GGGGTGGAAA CGTCGAGGTT TCGCCTAAGG ACGACCAGAT TGTCGTTGAT GGCGTCACTG TCATTGGTCG CAGACGCATT GAGACTCGCA TGCCCATTCA GGCGTCTGAG CTGTTCTCCA TGAACATATG CAACCTCCTT GAGGATCTTG GTGGTGGCAG CAACTTCCGC ATCAACATGG ACGACGAAGT CATCAGAGGA TTGGTCGCAG TCTACCAAGG TCGCAACGTG TGGCAGCCAT CGCAGCCCAC TCCTGTTTCC AGGACACCTC CGCGCGGCCA GATGCCGCCC CCGTCTGCAC CTGGTGCACC AGCTCCTGAG AAGCCTGGTG CCTTTGCTCA AGCACTTGCT TCGGATGCAT TCTTCGCAAT GTGTCTTGTT GTTGCTGCCG CTGTTGTCGG GCTCCTTGGC ATTGTCCTTG ACCCTGTGGA GCTCAAGCAT TTGACTCTCC TCGGCTTGTC TCTCATCGTC GGCTACTACT GCGTGTGGGC CGTTACGCCT TCGCTTCACA CACCATTGAT GTCTGTGACG AATGCCCTTT CGGGAGTCAT TGTCATCGGC TGCATGCTCG AGTACGGAAC CGCCATGATA TCCGGATTCA CTCTTCTCGC ACTCATTGGA ACCTTCTTGG CTTCCGTCAA CGTTGCTGGT GGATTCTTCG TAACTCACCG CATGCTGAAG ATGTTTCAGA TATAAGGGGA GAACCCCCTT GAGTTAATCT TAACTCAGAA TAACTCTTTT TCAATTGTAT AAACCTGTAC TCGTTGCAAA AAAAAAAGGA ATTC.
82 . The DNA sequence of claim 78 comprising all or part of the nucleotide sequence
CC CCAGACAGCT AAGCGTGGCA ACATTCTTGG TCTTGTGGGC ATGGTAGCCG CTGTCGTCGT GACCTTCACG GAGGCAGGGT TTGGACAGCA TTACTTGCTG TTTTTCGCCA CTGCTGCACC GGCCCTTGGC CTGGGGCTGT ACATTGCGCA GTCTGTCAAC ATGACTGAGA TGCCTCAACT CGTGGCTCTT TTCCACAGTT TCGTCGGTCT TGCCGCCGTA ATGGTTGGGT TCGCGAACTT CCACTCCCCT GCTGGCGTGG AGCGCGCTTC CTCACTTCTA CGTCTGTTGG AGGTCTACGC CGGCGTTTTC GTCGCCGGTA TCACCTTCAC CGGATCAGTG GTCGCTGCGG CAAAGCTCCA TGGATCGATG GAGAGCCGCT CATTGAGGGT TCCCGGACGC CATGCGTTGA ATACTGCCAC TATTGCTGCC ATTGGCGTAC TTGGCGCTCT TTTTTGCGTC TCTTCTGGCC ACTTTACACG CATGCTTTGC CTTTATGTGA ATGCTGGCTT GAGCATGTGG CTTGGTTTTC ACCTGGTCGC CGCTATTGGT GGAGCTGACA TGCCCGTCGT GATCAGCTTG CTGAACTCGT ATTCCGGAGT GGCGTTGGCT GCCAGTGGCT TCATGTTGGA CAACAACTTG CTGATCATTG CTGGTGCTCT CATCGCGTCA TCTGGTGCCA TTCTGTCTTA CATCATGTGC AAAGGCATGA ACCGGAGTCT GTGGAATGTC GTTCTTGGTG GCTTCGAGGA GGCCGAGGAC GTTGGCGCAG CCAGCCCTCA GGGGGCTGTG CAGCAGGCCA CGGCTGATCA GGTCGCCGAC GAGTTGCTGG CTGCCCGCAA AGTTTTGATC GTGCCTGGAT ACGGAATGGC CGTTGCAAGG TGCCAGAGCG AGCTTGCAGA CATTGCCAAG AACTTGATGA ACTGCGGTAT CACCGTCGAT TTCGGCATCC ATCCAGTTGC TGGTCGCATG CCAGGCCACA TGAACGTCCT CCTCGCTGAG GCTGATGTTC CGTACAAGAT TGTCAAGGAG ATGTCTGAAG TCAACCCGGA AATGAGCTCC TACGACGTCG TCCTGGTTGT TGGAGCCAAC GACACCGTCA ATCCTGCAGC CCTTGAGCCA GGATCAAAGA TCTCAGGAAT GCCCGTTATA GAGGCCTGGA AAGCTAGACG CGTTTTTGTG CTGAAGCGGT CCATGGCTGC TGGATATGCC AGCATTGAAA ATCCACTTTT CCATCTGGAG AACACACGCA TGCTCTTCGG AAACGCAAAG AACACCACTT CTGCAGTCTT CGCCCGTGTC AATGCCAGAG CCGAGCAAAT GCCACCATCT GCTGCCCGTG ATGACCTCGA AGCTGGACTA CTTGAGTTCG ATAGGGAAGA ACGTGTTGAT CCCTCTTCTT GGCCATATCC CAGGATGGCT GTTGGTGTTC,TGAGAGACTC CAATGGCTCT GTTATGGTGC CAGTAGCTCC GAAGTTTGTG CCCAAGCTGA GGAAGTTGGC ATTCCGTGTC AATGTCGAGT CTGGTGCTGG CGCCGATGCC GGCTTTACTG ACGAAGAGTA CAGGAGGGCT GGAGCAGAAG TCCTGTCGGG CCCCGATGCA GTCATTAACC AGTCTCAAGT CCTGCTCCGC GTTTCAGCGC CGTCGCCAGA TCTGGTTTCG CGCATTCCTA GGGACAAGGT CCTTATCAGT TACCTATTCC CCAGCATCAA CCAACAAGCT CTTGACATGC TAGCACGCCA AGGCGTCACC GCACTTGCTG TGGATGAGGT TCCTCGCGTC ACAAGAGCAC AGAAGCTAGA CGTGAAGTCT GCTATGCAAG GTCTCCAGGG ATACCGCGCC GTTATCGAAG CGTTCAACGC TCTTCCGAAG CTCAGCAAAG CATCTATCAG TGCTGCAGGC CGTGTAGAAG CCGCTAAAGT TTTCGTCATC GGTGCCGGTG TTGCTGGACT ACAGGCAATT TCTACCGCCC ATGGTTTGGG TGCACAAGTA 8TTGGCCACG ATGTGCGCTC TGCAACTCGT GAGGAAGTCG AATCTTGCGG TGGAAAGTTC ATTGGTTTGA GAATGGGAGA GGAGGGTGAA GTCCTCGGAG GATATGCACG CGAGATGGGT GATGCATATC AGAGAGCGCA ACGCGAGATG ATTGCCAACA CAATCAAGCA CTGCGATGTC GTCATCTGTA CCGCTGCTAT TCACGGCAGA CCTTCACCAA AGCTCATATC ACGCGACATG TTGCGTTCAA TGAAGCCTGG CTCCGTCGTC GTAGATCTTG CAACAGAGTT CGGTGATGTG CGCTCCGGCT GGGGTGGAAA CGTCGAGGTT TCGCCTAAGG ACGACCAGAT TGTCGTTGAT GGCGTCACTG TCATTGGTCG CAGACGCATT GAGACTCGCA TGCCCATTCA GGCGTCTGAG CTGTTCTCCA TGAACATATG CAACCTCCTT GAGGATCTTG GTGGTGGCAG CAACTTCCGC ATCAACATGG ACGACGAAGT CATCAGAGGA TTGGTCGCAG TCTACCAAGG TCGCAACGTG TGGCAGCCAT CGCAGCCCAC TCCTGTTTCC AGGACACCTC CGCGCGGCCA GATGCCGCCC CCGTCTGCAC CTGGTGCACC AGCTCCTGAG AAGCCTGGTG CCTTTGCTCA AGCACTTGCT TCGGATGCAT TCTTCGCAAT GTGTCTTGTT GTTGCTGCCG CTGTTGTCGG GCTCCTTGGC ATTGTCCTTG ACCCTGTGGA GCTCAAGCAT TTGACTCTCC TCGGCTTGTC TCTCATCGTC GGCTACTACT GCGTGTGGGC CGTTACGCCT TCGCTTCACA CACCATTGAT GTCTGTGACG AATGCCCTTT CGGGAGTCAT TGTCATCGGC TGCATGCTCG AGTACGGAAC CGCCATGATA TCCGGATTCA CTCTTCTCGC ACTCATTGGA ACCTTCTTGG CTTCCGTCAA CGTTGCTGGT GGATTCTTCG TAACTCACCG CATGCTGAAG ATGTTTCAGA TATAA.
83 . A recombinant vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of greater than 200 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712.
84 . The recombinant vector of claim 83 comprising a DNA sequence encoding a protein having the amino acid sequence
ProGlnThrAlaLysArgGlyAsnIleLeuGlyLeuValGlyMetValAlaAlaValVal ValThrPheThrGluAlaGlyPheGlyGlnHisTyrLeuLeuPhePheAlaThrAlaAla ProAlaLeuGlyLeuGlyLeuTyrIleAlaGlnSerValAsnMetThrGluMetProGln LeuValAlaLeuPheHisSerPheValGlyLeuAlaAlaValMetValGlyPheAlaAsn PheHisSerProAlaGlyValGluArgAlaSerSerLeuLeuArgLeuLeuGluValTyr AlaGlyValPheValAlaGlyIleThrPheThrGlySerValValAlaAlaAlaLysLeu HisGlySerMetGluSerArgSerLeuArgValProGlyArgHisAlaLeuAsnThrAla ThrIleAlaAlaIleGlyValLeuGlyAlaLeuPheCysValSerSerGlyHisPheThr ArgMetLeuCysLeuTyrValAsnAlaGlyLeuSerMetTrpLeuGlyPheHisLeuVal AlaAlaIleGlyGlyAlaAspMetProValValIleSerLeuLeuAsnSerTyrSerGly ValAlaLeuAlaAlaSerGlyPheMetLeuAspAsnAsnLeuLeuIleIleAlaGlyAla LeuIleAlaSerSerGlyAlaIleLeuSerTyrIleMetCysLysGlyMetAsnArgser LeuTrpAsnValValLeuGlyGlyPheGluGluAlaGluAspValGlyAlaAlaSerPro GlnGlyAlaValGlnGlnAlaThrAlaAspGlnValAlaAspGluLeuLeuAlaAlaArg LysValLeuIleValProGlyTyrGlyMetAlaValAlaArgCysGlnSerGluLeuAla AspIleAlaLysAsnLeuMetAsnCysGlyIleThrValAspPheGlyIleHisProVal AlaGlyArgMetProGlyHisMetAsnValLeuLeuAlaGluAlaAspValProTyrLys IleValLysGluMetSerGluValAsnProGluMetSerSerTyrAspValValLeuVal ValGlyAlaAsnAspThrValAsnProAlaAlaLeuGluProGlySerLysIleSerGly MetProValIleGluAlaTrpLysAlaArgArgValPheValLeuLysArgserMetAla AlaGlyTyrAlaSerIleGluAsnProLeuPheHisLeuGluAsnThrArgMetLeuPhe GlyAsnAlaLysAsnThrThrSerAlaValPheAlaArgValAsnAlaArgAlaGluGln MetProProSerAlaAlaArgAspAspLeuGluAlaGlyLeuLeuGluPheAspArgGlu GluArgValAspProSerSerTrpProTyrProArgMetAlaValGlyValLeuArgAsp SerAsnGlySerValMetValProValAlaProLysPheValProLysLeuArgLysLeu AlaPheArgValAsnValGluSerGlyAlaGlyAlaAspAlaGlyPheThrAspGluGlu TyrArgArgAlaGlyAlaGluValLeuSerGlyProAspAlaValIleAsnGlnSerGln ValLeuLeuArgValSerAlaProSerProAspLeuValSerArgIleProArgAspLys ValLeuIleSerTyrLeuPheProSerIleAsnGlnGlnAlaIeuAspMetLeuAlaArg GlnGlyValThrAlaLeuAlaValAspGluValProArgValThrArgAlaGlnLysLeu AspValLysSerAlaMetGlnGlyLeuGlnGlyTyrArgAlaValIleGluAlaPheAsn AlaLeuProLysLeuSerLysAlaSerIleSerAlaAlaGlyArgValGluAlaAlaLys ValPheValIleGlyAlaGlyValAlaGlyLeuGlnAlaIleSerThrAlaHisGlyLeu GlyAlaGlnVal???GlyHisAspValArgSerAlaThrArgGluGluValGluSerCys GlyGlyLysPheIleGlyLeuArgMetGlyGluGluGlyGluValLeuGlyGlyTyrAla ArgGluMetGlyAspAlaTyrGlnArgAlaGlnArgGluMetIleAlaAsnThrIleLys HisCysAspValValIleCysThrAlaAlaIleHisGlyArgProSerProLysLeuIle SerArgAspMetLeuArgSerMetLysProGlySerValValValAspLeuAlaThrGlu PheGlyAspValArgSerGlyTrpGlyGlyAsnValGluValSerProLysAspAspGln IleValValAspGlyValThrValIleGlyArgArgArgIleGluThrArgMetproIle GlnAlaSerGluLeuPheSerMetAsnIleCysAsnLeuLeuGluAspLeuGlyGlyGly SerAsnPheArgIleAsnMetAspAspGluValIleArgGlyLeuValAlaValTyrGln GlyArgAsnValTrpGlnProSerGlnProThrProValSerArgThrProProArgGly GlnMetProProProSerAlaProGlyAlaProAlaProGluLysProGlyAlaPheAla GlnAlaLeuAlaSerAspAlaPhePheAlaMetCysLeuValValAlaAlaAlaValVal GlyLeuLeuGlyIleValLeuAspProValGluLeuLysHisLeuThrLeuLeuGlyLeu SerLeuIleValGlyTyrTyrCysValTrpAlaValThrProSerLeuHisThrProLeu MetSerValThrAsnAlaLeuSerGlyVallleValIleGlyCysMetLeuGluTyrGly ThrAlaMetIleSerGlyPheThrLeuLeuAlaLeuIleGlyThrPheLeuAlaSerVal AsnValAlaGlyGlyPhePheValThrHisArgMetLeuLysMetPheGlnIle
or a functional equivalent or partial sequence thereof.
85 . The recombinant vector of claim 83 comprising a DNA sequence encoding a protein having the amino acid sequence
ThrAlaLysArgGlyAsnIleLeuGlyLeuValGlyMetValAlaAlaValVal ValThrPheThrGluAlaGlyPheGlyGlnHisTyrLeuLeuPhePheAlaThrAlaAla ProAlaLeuGlyLeuGlyLeuTyrIleAlaGlnSerValAsnMetThrGluMetProGln LeuValAlaLeuPheHisSerPheValGlyLeuAlaAlaValMetValGlyPheAlaAsn PheHisSerProAlaGlyValGluArgAlaSerSerLeuLeuArgLeuLeuGluValTyr AlaGlyValPheValAlaGlyIleThrPheThrGlySerValValAlaAlaAlaLysLeu HisGlySerMetGluSerArgSerLeuArgValProGlyArgHisAlaLeuAsnThrAla ThrIleAlaAlaIleGlyValLeuGlyAlaLeuPheCysValSerSerGlyHisPheThr ArgMetLeuCysLeuTyrValAsnAlaGlyLeuSerMetTrpLeuGlyPheHisLeuVal AlaAlaIleGlyGlyAlaAspMetProValValIleSerLeuLeuAsnSerTyrSerGly ValAlaLeuAlaAlaSerGlyPheMetLeuAspAsnAsnLeuLeuIleIleAlaGlyAla LeuIleAlaSerSerGlyAlaIleLeuSerTyrIleMetCysLysGlyMetAsnArgser LeuTrpAsnValValLeuGlyGlyPheGluGluAlaGluAspValGlyAlaAlaSerPro GlnGlyAlaValGlnGlnAlaThrAlaAspGlnValAlaAspGluLeuLeuAlaAlaArg LysValLeuIleValProGlyTyrGlyMetAlaValAlaArgCysGlnSerGluLeuAla AspIleAlaLysAsnLeuMetAsnCysGlyIleThrValAspPheGlyIleHisProVal AlaGlyArgMetProGlyHisMetAsnValLeuLeuAlaGluAlaAspValProTyrLys IleValLysGluMetSerGluValAsnProGluMetSerSerTyrAspValValLeuVal ValGlyAlaAsnAspThrValAsnProAlaAlaLeuGluProGlySerLysIleSerGly MetProValIleGluAlaTrpLysAlaArgArgValPheValLeuLysArgSerMetAla AlaGlyTyrAlaSerIleGluAsnProLeuPheHisLeuGluAsnThrArgMetLeuPhe GlyAsnAlaLysAsnThrThrSerAlaValPheAlaArgValAsnAlaArgAlaGluGln MetProProSerAlaAlaArgAspAspLeuGluAlaGlyLeuLeuGluPheAspArgGlu GluArgValAspProSerSerTrpProTyrProArgMetAlaValGlyValLeuArgAsp SerAsnGlySerValMetValProValAlaProLysPheValProLysLeuArgLysLeu AlaPheArgValAsnValGluSerGlyAlaGlyAlaAspAlaGlyPheThrAspGluGlu TyrArgArgAlaGlyAlaGluValLeuSerGlyProAspAlaValIleAsnGlnSerGln ValLeuLeuArgValSerAlaProSerProAspLeuValSerArgIleProArgAspLys ValLeuIleSerTyrLeuPheProSerIleAsnGlnGlnAlaLeuAspMetLeuAlaArg GlnGlyValThrAlaLeuAlaValAspGluValProArgValThrArgAlaGlnLysLeu AspValLysSerAlaMetGlnGlyLeuGlnGlyTyrArgAlaValIleGluAlaPheAsn AlaLeuProLysLeuSerLysAlaSerIleSerAlaAlaGlyArgValGluAlaAlaLys ValPheValIleGlyAlaGlyValAlaGlyLeuGlnAlaIleSerThrAlaHisGlyLeu GlyAlaGlnValPheGlyHisAspValArgSerAlaThrArgGluGluValGluSerCys GlyGlyLysPheIleGlyLeuArgMetGlyGluGluGlyGluValLeuGlyGlyTyrAla ArgGluMetGlyAspAlaTyrGlnArgAlaGlnArgGluMetIleAlaAsnThrIleLys HisCysAspValValIleCysThrAlaAlaIleHisGlyArgProSerProLysLeuIle SerArgAspMetLeuArgSerMetLysProGlySerValValValAspLeuAlaThrGlu PheGlyAspValArgSerGlyTrpGlyGlyAsnValGluValSerProLysAspAspGln IleValValAspGlyValThrValIleGlyArgArgArgIleGluThrArgMetProIle GlnAlaSerGluLeuPheSerMetAsnIleCysAsnLeuLeuGluAspLeuGlyGlyGly SerAsnPheArgIleAsnMetAspAspGluValIleArgGlyLeuValAlaValTyrGln GlyArgAsnValTrpGlnProSerGlnProThrProValSerArgThrProProArgGly GlnMetProProProSerAlaProGlyAlaProAlaProGluLysProGlyAlaPheAla GlnAlaLeuAlaSerAspAlaPhePheAlaMetCysLeuValValAlaAlaAlaValVal GlyLeuLeuGlyIleValLeuAspProValGluLeuLysHisLeuThrLeuLeuGlyLeu SerLeuIleValGlyTyrTyrCysValTrpAlaValThrProSerLeuHisThrProLeu MetSerValThrAsnAlaLeuSerGlyValIleValIleGlyCysMetLeuGluTyrGly ThrAlaMetIleSerGlyPheThrLeuLeuAlaLeuIleGlyThrPheLeuAlaSerVal AsnValAlaGlyGlyPhePheValThrHisArgMetLeuLysMetPheGlnIle
or a functional equivalent or partial sequence thereof.
86 . The recombinant vector of claim 83 comprising all or part of the nucleotide sequence
GAATTCCGCC CCAGACAGCT AAGCGTGGCA ACATTCTTGG TCTTGTGGGC ATGGTAGCCG CTGTCGTCGT GACCTTCACG GAGGCAGGGT TTGGACAGCA TTACTTGCTG TTTTTCGCCA CTGCTGCACC GGCCCTTGGC CTGGGGCTGT ACATTGCGCA GTCTGTCAAC ATGACTGAGA TGCCTCAACT CGTGGCTCTT TTCCACAGTT TCGTCGGTCT TGCCGCCGTA ATGGTTGGGT TCGCGAACTT CCACTCCCCT GCTGGCGTGG AGCGCGCTTC CTCACTTCTA CGTCTGTTGG AGGTCTACGC CGGCGTTTTC GTCGCCGGTA TCACCTTCAC CGGATCAGTG GTCGCTGCGG CAAAGCTCCA TGGATCGATG GAGAGCCGCT CATTGAGGGT TCCCGGACGC CATGCGTTGA ATACTGCCAC TATTGCTGCC ATTGGCGTAC TTGGCGCTCT TTTTTGCGTC TCTTCTGGCC ACTTTACACG CATGCTTTGC CTTTATGTGA ATGCTGGCTT GAGCATGTGG CTTGGTTTTC ACCTGGTCGC CGCTATTGGT GGAGCTGACA TGCCCGTCGT GATCAGCTTG CTGAACTCGT ATTCCGGAGT GGCGTTGGCT GCCAGTGGCT TCATGTTGGA CAACAACTTG CTGATCATTG CTGGTGCTCT CATCGCGTCA TCTGGTGCCA TTCTGTCTTA CATCATGTGC AAAGGCATGA ACCGGAGTCT GTGGAATGTC GTTCTTGGTG GCTTCGAGGA GGCCGAGGAC GTTGGCGCAG CCAGCCCTCA GGGGGCTGTG CAGCAGGCCA CGGCTGATCA GGTCGCCGAC GAGTTGCTGG CTGCCCGCAA AGTTTTGATC GTGCCTGGAT ACGGAATGGC CGTTGCAAGG TGCCAGAGCG AGCTTGCAGA CATTGCCAAG AACTTGATGA ACTGCGGTAT CACCGTCGAT TTCGGCATCC ATCCAGTTGC TGGTCGCATG CCAGGCCACA TGAACGTCCT CCTCGCTGAG GCTGATGTTC CGTACAAGAT TGTCAAGGAG ATGTCTGAAG TCAACCCGGA AATGAGCTCC TACGACGTCG TCCTGGTTGT TGGAGCCAAC GACACCGTCA ATCCTGCAGC CCTTGAGCCA GGATCAAAGA TCTCAGGAAT GCCCGTTATA GAGGCCTGGA AAGCTAGACG CGTTTTTGTG CTGAAGCGGT CCATGGCTGC TGGATATGCC AGCATTGAAA ATCCACTTTT CCATCTGGAG AACACACGCA TGCTCTTCGG AAACGCAAAG AACACCACTT CTGCAGTCTT CGCCCGTGTC AATGCCAGAG CCGAGCAAAT GCCACCATCT GCTGCCCGTG ATGACCTCGA AGCTGGACTA CTTGAGTTCG ATAGGGAAGA ACGTGTTGAT CCCTCTTCTT GGCCATATCC CAGGATGGCT GTTGGTGTTC TGAGAGACTC CAATGGCTCT GTTATGGTGC CAGTAGCTCC GAAGTTTGTG CCCAAGCTGA GGAAGTTGGC ATTCCGTGTC AATGTCGAGT CTGGTGCTGG CGCCGATGCC GGCTTTACTG ACGAAGAGTA CAGGAGGGCT GGAGCAGAAG TCCTGTCGGG CCCCGATGCA GTCATTAACC AGTCTCAAGT CCTGCTCCGC GTTTCAGCGC CGTCGCCAGA TCTGGTTTCG CGCATTCCTA GGGACAAGGT CCTTATCAGT TACCTATTCC CCAGCATCAA CCAACAAGCT CTTGACATGC TAGCACGCCA AGGCGTCACC GCACTTGCTG TGGATGAGGT TCCTCGCGTC ACAAGAGCAC AGAAGCTAGA CGTGAAGTCT GCTATGCAAG GTCTCCAGGG ATACCGCGCC GTTATCGAAG CGTTCAACGC TCTTCCGAAG CTCAGCAAAG CATCTATCAG TGCTGCAGGC CGTGTAGAAG CCGCTAAAGT TTTCGTCATC GGTGCCGGTG TTGCTGGACT ACAGGCAATT TCTACCGCCC ATGGTTTGGG TGCACAAGTA 8TTGGCCACG ATGTGCGCTC TGCAACTCGT GAGGAAGTCG AATCTTGCGG TGGAAAGTTC ATTGGTTTGA GAATGGGAGA GGAGGGTGAA GTCCTCGGAG GATATGCACG CGAGATGGGT GATGCATATC AGAGAGCGCA ACGCGAGATG ATTGCCAACA CAATCAAGCA CTGCGATGTC GTCATCTGTA CCGCTGCTAT TCACGGCAGA CCTTCACCAA AGCTCATATC ACGCGACATG TTGCGTTCAA TGAAGCCTGG CTCCGTCGTC GTAGATCTTG CAACAGAGTT CGGTGATGTG CGCTCCGGCT GGGGTGGAAA CGTCGAGGTT TCGCCTAAGG ACGACCAGAT TGTCGTTGAT GGCGTCACTG TCATTGGTCG CAGACGCATT GAGACTCGCA TGCCCATTCA GGCGTCTGAG CTGTTCTCCA TGAACATATG CAACCTCCTT GAGGATCTTG GTGGTGGCAG CAACTTCCGC ATCAACATGG ACGACGAAGT CATCAGAGGA TTGGTCGCAG TCTACCAAGG TCGCAACGTG TGGCAGCCAT CGCAGCCCAC TCCTGTTTCC AGGACACCTC CGCGCGGCCA GATGCCGCCC CCGTCTGCAC CTGGTGCACC AGCTCCTGAG AAGCCTGGTG CCTTTGCTCA AGCACTTGCT TCGGATGCAT TCTTCGCAAT GTGTCTTGTT GTTGCTGCCG CTGTTGTCGG GCTCCTTGGC ATTGTCCTTG ACCCTGTGGA GCTCAAGCAT TTGACTCTCC TCGGCTTGTC TCTCATCGTC GGCTACTACT GCGTGTGGGC CGTTACGCCT TCGCTTCACA CACCATTGAT GTCTGTGACG AATGCCCTTT CGGGAGTCAT TGTCATCGGC TGCATGCTCG AGTACGGAAC CGCCATGATA TCCGGATTCA CTCTTCTCGC ACTCATTGGA ACCTTCTTGG CTTCCGTCAA CGTTGCTGGT GGATTCTTCG TAACTCACCG CATGCTGAAG ATGTTTCAGA TATAAGGGGA GAACCCCCTT GAGTTAATCT TAACTCAGAA TAACTCTTTT TCAATTGTAT AAACCTGTAC TCGTTGCAAA AAAAAAAGGA ATTC.
87 . The recombinant vector of claim 83 comprising all or part of the nucleotide sequence
CC CCAGACAGCT AAGCGTGGCA ACATTCTTGG TCTTGTGGGC ATGGTAGCCG CTGTCGTCGT GACCTTCACG GAGGCAGGGT TTGGACAGCA TTACTTGCTG TTTTTCGCCA CTGCTGCACC GGCCCTTGGC CTGGGGCTGT ACATTGCGCA GTCTGTCAAC ATGACTGAGA TGCCTCAACT CGTGGCTCTT TTCCACAGTT TCGTCGGTCT TGCCGCCGTA ATGGTTGGGT TCGCGAACTT CCACTCCCCT GCTGGCGTGG AGCGCGCTTC CTCACTTCTA CGTCTGTTGG AGGTCTACGC CGGCGTTTTC GTCGCCGGTA TCACCTTCAC CGGATCAGTG GTCGCTGCGG CAAAGCTCCA TGGATCGATG GAGAGCCGCT CATTGAGGGT TCCCGGACGC CATGCGTTGA ATACTGCCAC TATTGCTGCC ATTGGCGTAC TTGGCGCTCT TTTTTGCGTC TCTTCTGGCC ACTTTACACG CATGCTTTGC CTTTATGTGA ATGCTGGCTT GAGCATGTGG CTTGGTTTTC ACCZGGTCGC CGCTATTGGT GGAGCTGACA TGCCCGTCGT GATCAGCTTG CTGAACTCGT ATTCCGGAGT GGCGTTGGCT GCCAGTGGCT TCATGTTGGA CAACAACTTG CTGATCATTG CTGGTGCTCT CATCGCGTCA TCTGGTGCCA TTCTGTCTTA CATCATGTGC AAAGGCATGA ACCGGAGTCT GTGGAATGTC GTTCTTGGTG GCTTCGAGGA GGCCGAGGAC GTTGGCGCAG CCAGCCCTCA GGGGGCTGTG CAGCAGGCCA CGGCTGATCA GGTCGCCGAC GAGTTGCTGG CTGCCCGCAA AGTTTTGATC GTGCCTGGAT ACGGAATGGC CGTTGCAAGG TGCCAGAGCG AGCTTGCAGA CATTGCCAAG AACTTGATGA ACTGCGGTAT CACCGTCGAT TTCGGCATCC ATCCAGTTGC TGGTCGCATG CCAGGCCACA TGAACGTCCT CCTCGCTGAG GCTGATGTTC CGTACAAGAT TGTCAAGGAG ATGTCTGAAG TCAACCCGGA AATGAGCTCC TACGACGTCG TCCTGGTTGT TGGAGCCAAC GACACCGTCA ATCCTGCAGC CCTTGAGCCA GGATCAAAGA TCTCAGGAAT GCCCGTTATA GAGGCCTGGA AAGCTAGACG CGTTTTTGTG CTGAAGCGGT CCATGGCTGC TGGATATGCC AGCATTGAAA ATCCACTTTT CCATCTGGAG AACACACGCA TGCTCTTCGG AAACGCAAAG AACACCACTT CTGCAGTCTT CGCCCGTGTC AATGCCAGAG CCGAGCAAAT GCCACCATCT GCTGCCCGTG ATGACCTCGA AGCTGGACTA CTTGAGTTCG ATAGGGAAGA ACGTGTTGAT CCCTCTTCTT GGCCATATCC CAGGATGGCT GTTGGTGTTC TGAGAGACTC CAATGGCTCT GTTATGGTGC CAGTAGCTCC GAAGTTTGTG CCCAAGCTGA GGAAGTTGGC ATTCCGTGTC AATGTCGAGT CTGGTGCTGG CGCCGATGCC GGCTTTACTG ACGAAGAGTA CAGGAGGGCT GGAGCAGAAG TCCTGTCGGG CCCCGATGCA GTCATTAACC AGTCTCAAGT CCTGCTCCGC GTTTCAGCGC CGTCGCCAGA TCTGGTTTCG CGCATTCCTA GGGACAAGGT CCTTATCAGT TACCTATTCC CCAGCATCAA CCAACAAGCT CTTGACATGC TAGCACGCCA AGGCGTCACC GCACTTGCTG TGGATGAGGT TCCTCGCGTC ACAAGAGCAC AGAAGCTAGA CGTGAAGTCT GCTATGCAAG GTCTCCAGGG ATACCGCGCC GTTATCGAAG CGTTCAACGC TCTTCCGAAG CTCAGCAAAG CATCTATCAG TGCTGCAGGC CGTGTAGAAG CCGCTAAAGT TTTCGTCATC GGTGCCGGTG TTGCTGGACT ACAGGCAATT TCTACCGCCC ATGGTTTGGG TGCACAAGTA 8TTGGCCACG ATGTGCGCTC TGCAACTCGT GAGGAAGTCG AATCTTGCGG TGGAAAGTTC ATTGGTTTGA GAATGGGAGA GGAGGGTGAA GTCCTCGGAG GATATGCACG CGAGATGGGT GATGCATATC AGAGAGCGCA ACGCGAGATG ATTGCCAACA CAATCAAGCA CTGCGATGTC GTCATCTGTA CCGCTGCTAT TCACGGCAGA CCTTCACCAA AGCTCATATC ACGCGACATG TTGCGTTCAA TGAAGCCTGG CTCCGTCGTC GTAGATCTTG CAACAGAGTT CGGTGATGTG CGCTCCGGCT GGGGTGGAAA CGTCGAGGTT TCGCCTAAGG ACGACCAGAT TGTCGTTGAT GGCGTCACTG TCATTGGTCG CAGACGCATT GAGACTCGCA TGCCCATTCA GGCGTCTGAG CTGTTCTCCA TGAACATATG CAACCTCCTT GAGGATCTTG GTGGTGGCAG CAACTTCCGC ATCAACATGG ACGACGAAGT CATCAGAGGA TTGGTCGCAG TCTACCAAGG TCGCAACGTG TGGCAGCCAT CGCAGCCCAC TCCTGTTTCC AGGACACCTC CGCGCGGCCA GATGCCGCCC CCGTCTGCAC CTGGTGCACC AGCTCCTGAG AAGCCTGGTG CCTTTGCTCA AGCACTTGCT TCGGATGCAT TCTTCGCAAT GTGTCTTGTT GTTGCTGCCG CTGTTGTCGG GCTCCTTGGC ATTGTCCTTG ACCCTGTGGA GCTCAAGCAT TTGACTCTCC TCGGCTTGTC TCTCATCGTC GGCTACTACT GCGTGTGGGC CGTTACGCCT TCGCTTCACA CACCATTGAT GTCTGTGACG AATGCCCTTT CGGGAGTCAT TGTCATCGGC TGCATGCTCG AGTACGGAAC CGCCATGATA TCCGGATTCA CTCTTCTCGC ACTCATTGGA ACCTTCTTGG CTTCCGTCAA CGTTGCTGGT GGATTCTTCG TAACTCACCG CATGCTGAAG ATGTTTCAGA TATAA.
88 . The recombinant vector of claim 83 which recombinant vector is an E. coli vector.
89 . The recombinant vector of claim 83 which is a pox virus vector.
90 . A microorganism transformed with a recombinant vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of greater than 200 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712.
91 . A method for producing a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of greater than 200 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712, which process comprises:
(a) culturing a microorganism transformed with a recombinant vector comprising a DNA sequence encoding the said protein in a culture medium under conditions in which the DNA sequence is expressed; (b) isolating the said protein from the culture.
92 . A vaccine for protecting poultry against coccidiosis comprising a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of greater than 200 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712 and a physiologically acceptable carrier.
93 . A vaccine for protecting poultry against coccidiosis containing a recombinant poxvirus vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of greater than 200 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712 and a physiologically acceptable carrier.
94 . A method for protecting poultry against coccidiosis, which method comprises administering an effective amount of a vaccine comprising a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of greater than 200 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712 and a physiologically acceptable carrier to a young fowl that is susceptible to coccidiosis.
95 . A method for protecting poultry against coccidiosis, which method comprises administering an effective amount of a vaccine for protecting poultry against coccidiosis containing a recombinant poxvirus vector comprising a DNA sequence encoding a protein having one or more immunoreactive and/or antigenic determinants of an Eimeria surface antigen, which surface antigen has an apparent molecular weight of greater than 200 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712 and a physiologically acceptable carrier to a young fowl that is susceptible to coccidiosis.
96 . An isolated antibody which is directed against an Eimeria surface antigen, which surface antigen has an apparent molecular weight of greater than 200 kilodaltons and specifically binds to the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712.
97 . The antibody of claim 96 which is a monoclonal antibody, such as the monoclonal antibody deposited with the American Type Culture Collection and assigned accession No. HB 9712.Cited by (0)
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