Nanoemulsion comprising metabolites of ginseng saponin as an active component and a method for preparing the same, and a skin-care composition for anti-aging containing the same

Abstract

Disclosed herein is nanoemulsion prepared by emulsifying main metabolites of ginseng saponin obtained by conversion of glucose, i.e. compound K (20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol), ginsenoside F1 (20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol) and compound Y (20-O-[α-L-arabinopyranosyl(1→6)-β-D-glucopyranosyl]-20(S)-protopanaxadiol); and admixture thereof, in fine emulsion or liposome with dermotropic emulsifier by nano-emulsification; and having enhanced skin penetration, so to be effective in promoting proliferation of fibroblast and biosynthesis of collagen.

What claimed is:  
     
         1 . A nanoemulsion comprising metabolite of ginseng saponin as an effective component, wherein said metabolite of ginseng saponin is obtained by conversion of glucose from ginseng saponin.  
     
     
         2 . The nanoemulsion according to  claim 1 , wherein said metabolite of ginseng saponin is selected from the group consisting of 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol represented by the following formula 1; 20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol represented by the following formula 2; and 20-O-[α-L-arabinopyranosyl(1→6)-β-D-glucopyranosyl]-20(S)-protopanaxadiol represented by the following formula 3; and admixture thereof:  
       3.  
       
         
           
           
               
               
           
         
       
       (wherein, R 1  is O-Glc, R 2  is OH and R 3  is H).  
       
         
           
           
               
               
           
         
       
       (wherein, R 1  is O-Glc, R 2  is OH and R 3  is OH).  
       
         
           
           
               
               
           
         
       
       (wherein, R 1  is O-Glc 6 - 1 Arap, R 2  is OH and R 3  is H).  
     
     
         3 . The nanoemulsion according to  claim 2 , wherein said admixture comprises 30˜50 wt % of 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, 5˜25 wt % of 20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol and 5˜25 wt % of 20-O-[α-L-arabinopyranosyl(1→6)-β-D-glucopyranosyl]-20(S)-protopanaxadiol as main components.  
     
     
         4 . The nanoemulsion according to  claim 1 , which comprises said metabolite of ginseng saponin in an amount of 10 −10 ˜50% by weight based on the total weight of nanoemulsion.  
     
     
         5 . The nanoemulsion according to  claim 4 , which comprises said metabolite of ginseng saponin in an amount of 0.001˜30% by weight based on the total weight of nanoemulsion.  
     
     
         6 . The nanoemulsion according to  claim 1 , which has the diameter of 30˜500 nm.  
     
     
         7 . The nanoemulsion according to  claim 1 , which is emulsified with lecithin or its derivatives.  
     
     
         8 . The nanoemulsion according to  claim 7 , wherein said lecithin is a liposome containing at lease one selected from the group consisting of unsaturated choline compound, serine compound, cephalin compound and hydrogenated compounds thereof, and is employed in an amount of 0.5˜10% by weight based on the total weight of nanoemulsion.  
     
     
         9 . The nanoemulsion according to claims  8 , wherein said unsaturated choline compound is phosphatidylcholine or lysophosphatidylcholine; and said cephalin compound is phosphatidylethanolamine.  
     
     
         10 . The nanoemulsion according to any one of claims  7 , wherein at least one supplementary emulsifier selected from the group consisting of anionic, cationic, nonionic and amphoteric emulsifier is employed together with lecithin, in a ratio of 0.5˜5 times based on the weight of lecithin.  
     
     
         11 . The nanoemulsion according to  claim 1 , which is emulsified by nano-emulsification of homogenization under 500˜2,500 bar.  
     
     
         12 . A method for preparing the nanoemulsion according to  claim 1 , which comprises a step of emulsifying said metabolite of ginseng saponin with lecithin or its derivatives.  
     
     
         13 . The method according to  claim 12 , which comprises a step of emulsifying said metabolite of ginseng saponin by nano-emulsification.  
     
     
         14 . The method according to  claim 13 , wherein said nano-emulsification is homogenization under 500˜2,500 bar.  
     
     
         15 . A skin-care composition for anti-aging containing a nanoemulsion that comprises metabolite of ginseng saponin as an effective component in an amount of 10 −8 ˜50% by weight based on the total weight of composition, wherein said metabolite of ginseng saponin is obtained by conversion of glucose from ginseng saponin.  
     
     
         16 . The skin-care composition according to  claim 15 , wherein said metabolite of ginseng saponin is selected from the group consisting of 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol represented by the following formula 1; 20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol represented by the following formula 2; and 20-O-[α-L-arabinopyranosyl(1→6)-β-D-glucopyranosyl]-20(S)-protopanaxadiol represented by the following formula 3; and admixture thereof:  
       
         
           
           
               
               
           
         
       
       (wherein, R 1  is O-Glc, R 2  is OH and R 3  is H).  
       
         
           
           
               
               
           
         
       
       (wherein, R 1  is O-Glc, R 2  is OH and R 3  is OH).  
       
         
           
           
               
               
           
         
       
       (wherein, R 1  is O-Glc 6 - 1 Arap, R 2  is OH and R 3  is H).  
     
     
         17 . The skin-care composition according to  claim 16 , wherein said admixture comprises 30˜50 wt % of 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, 5˜25 wt % of 20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol and 5˜25 wt % of 20-O-[α-L-arabinopyranosyl(1→6)-β-D-glucopyranosyl]-20(S)-protopanaxadiol as main components.  
     
     
         18 . The skin-care composition according to  claim 15 , wherein said nanoemulsion comprises said metabolite of ginseng saponin in an amount of 10 −10 ˜50% by weight based on the total weight of nanoemulsion.  
     
     
         19 . The skin-care composition according to  claim 15 , wherein said nanoemulsion has the diameter of 30˜500 nm.  
     
     
         20 . The composition according to  claim 15 , which has a formulation selected from the group consisting of skin softeners, astringents, nutrient toilet water, nutrient creams, massage creams, essences, eye creams, eye essences, cleansing creams, cleansing foams, cleansing water, packs, powders, body lotions, body creams, body oils, body essences, make-up bases, foundation, hairdyes, shampoos, rinses, body cleansers, toothpastes, oral cleaning fluid, lotions, ointments, gels, creams, patches and sprays.