Nucleic acid sequences that encode a cell growth regulatory protein and uses thereof

Abstract

Compositions of matter consisting of a family of related nucleic acid sequences that encode proteins, termed Cell Growth Regulatory Proteins, that phosphorylate cell cycle targets, and methods of using the nucleotide sequences and the proteins encoded thereby, to diagnose and/or treat disease where the Cell Growth Regulatory Proteins have an apparent molecular weight of about 54.6 kdaltons.

1 . A composition of matter comprising an isolated nucleic acid sequence that encodes a human Cell Growth Regulatory Protein.  
     
     
         2 . A composition of matter as described in  claim 1  wherein said isolated nucleic acid sequence encodes a human Cell Growth Regulatory Protein with a transmembrane region.  
     
     
         3 . A composition of matter as described in  claim 2  wherein said isolated nucleic acid sequence encodes a human Cell Growth Regulatory Protein with a molecular weight of about 54.6 kd.  
     
     
         4 . A composition of matter comprising an isolated nucleic acid sequence as described in  claim 3  wherein said nucleic sequence is a cDNA sequence.  
     
     
         5 . A composition of matter as described in  claim 4  wherein said isolated nucleic acid cDNA sequence encoding said human Cell Growth Regulatory Protein comprises an isolated nucleic acid fragment.  
     
     
         6 . A nucleic acid sequence hybridizable under high stringency conditions to said nucleic acid sequence that encodes a human Cell Growth Regulatory Protein of  claim 4 .  
     
     
         7 . A nucleic acid sequence that encodes a human Cell Growth Regulatory Protein, wherein said nucleic acid sequence is about 90% homologous to said nucleic acid sequence of  claim 4 .  
     
     
         8 . A nucleic acid sequence that encodes a human Cell Growth Regulatory Protein, wherein said nucleic acid sequence is about 90% homologous to said nucleic acid sequence of  claim 4 .  
     
     
         9 . Isolated host cells comprising an isolated nucleic acid sequence of  claim 4  which encode a human Cell Growth Regulatory Protein.  
     
     
         10 . Vectors that comprise an isolated nucleic acid sequence of  claim 4  which encodes a human Cell Growth Regulatory Protein.  
     
     
         11 . A nucleic acid sequence on deposit with the ATCC with accession no. 98169 which encodes a human Cell Growth Regulatory Protein.  
     
     
         12 . A nucleic acid sequence that encodes a human Cell Growth Regulatory Protein, wherein said nucleic acid sequence is about 95% homologous to said nucleic acid sequence of  claim 11  on deposit with the ATCC with accession no. 98169.  
     
     
         13 . A nucleic acid sequence that encodes a human Cell Growth Regulatory Protein, wherein said nucleic acid sequence is about 90% homologous to said nucleic acid sequence of  claim 11  on deposit with the ATCC with accession no. 98169.  
     
     
         14 . A nucleic acid sequence that encodes a human Cell Growth Regulatory Protein, wherein said nucleic acid sequence encodes human Cell Growth Regulatory Proteins is selected from the groups consisting of Cell Growth Regulatory Protein-1b (residues 9-499), Cell Growth Regulatory Protein-1A (residues 58-499), Cell Growth Regulatory Protein-4b (residues 9-292), and Cell Growth Regulatory Protein-ΔT.M. (residues 9-499 with residues 379-398 deleted).  
     
     
         15 . A composition of matter comprising a human Cell Growth Regulatory Protein.  
     
     
         16 . A composition of matter as described in  claim 15  wherein said human Cell Growth Regulatory Protein has a molecular weight of about 54.6 k daltons.  
     
     
         17 . A composition of matter as described in  claim 15  wherein said human Cell Growth Regulatory Protein is selected from the groups consisting of Cell Growth Regulatory Protein-1b (residues 9-499), Cell Growth Regulatory Protein-1A (residues 58-499), Cell Growth Regulatory Protein-4b (residues 9-292), and Cell Growth Regulatory Protein-ΔT.M. (residues 9-499 with residues 379-398 deleted).  
     
     
         18 . Isolated host cells comprising an isolated nucleic acid sequence of  claim 11  which encodes a Cell Growth Regulatory Protein.  
     
     
         19 . A complex comprising a substantially purified cell cycle target and said Cell Growth Regulatory Protein of  claim 15 .  
     
     
         20 . A complex comprising a substantially purified cell cycle target and a Cell Growth Regulatory Protein selected from the groups consisting of Cell Growth Regulatory Protein-1b (residues 9-499), Cell Growth Regulatory Protein-1A (residues 58-499), Cell Growth Regulatory Protein-4b (residues 9-292), and Cell Growth Regulatory Protein-ΔT.M. (residues 9-499 with residues 379-398 deleted).  
     
     
         21 . A host cell comprising said complex of  claim 19 .  
     
     
         22 . A host cell comprising said complex of  claim 20 .  
     
     
         23 . A method of forming a complex comprising a cell cycle target and Cell Growth Regulatory Protein, comprising contacting in solution said cell cycle target with said Cell Growth Regulatory Protein, allowing sufficient time for said complex to form, and removing uncomplexed cell cycle target and Cell Growth Regulatory Protein.  
     
     
         24 . Isolated antibody that binds to said Cell Growth Regulatory Protein of  claim 15 .  
     
     
         25 . A pharmaceutical composition comprising a Cell Growth Regulatory Protein encoded by a nucleic acid on deposit with the ATCC, Accession Number 98169.  
     
     
         26 . A process for producing a Cell Growth Regulatory Protein comprising culturing cells of  claim 9  in a suitable culture medium and isolating said protein from said cells or said medium.  
     
     
         27 . A method of identifying compounds that affect the interaction of a Cell Growth Regulatory Protein with a cell cycle target, comprising the steps of: 
 contacting said Cell Growth Regulatory Protein with said cell cycle target in the presence or absence of said compounds and under conditions that favor the phosphoylation of said cell cycle target; and    measuring the effect of said compounds on the phosphorylation of said cell cycle target.    
     
     
         28 . A method as described in  claim 27 , wherein said Cell Cycle Growth Regulatory Protein is encoded by a nucleic acid sequence on deposit with the ATCC with accession no. 98169.  
     
     
         29 . A method as described in  claim 28 , wherein said Cell Cycle Growth Regulatory Protein is encoded by a nucleic acid sequence that is about 95% or 90% homologous to said nucleic acid sequence on deposit with the ATCC with accession no. 98169.  
     
     
         30 . A method of identifying compounds that affect the interaction of a Cell Growth Regulatory Protein with a cell cycle target having enzymatic activity, comprising the steps of: 
 contacting said Cell Growth Regulatory Protein with said cell cycle target in the presence or absence of said compounds and under conditions that favor the phosphoylation of said cell cycle target; and    measuring the effect of said compounds on the enzymatic activity of said cell cycle target.    
     
     
         31 . A method as described in  claim 29 , wherein said Cell Cycle Growth Regulatory Protein is encoded by a nucleic acid sequence on deposit with the ATCC with accession no. 98169.  
     
     
         32 . A method as described in  claim 30 , wherein said Cell Cycle Growth Regulatory Protein is encoded by a nucleic acid sequence that is about 95% or 90% homologous to said nucleic acid sequence on deposit with the ATCC with accession no. 98169.