Protein tyrosine kinases

Abstract

The protein tyrosine kinase receptors, designated Rse and HPTK6, have been purified from human and/or murine cell tissues. Rse and HPTK6 have been cloned from a cDNA library of a human liver carcinoma cell line (i.e., Hep 3B) using PCR amplification. Provided herein are nucleic acid sequences encoding Rse and HPTK6 useful as diagnostics and in the recombinant preparation of Rse and HPTK6. Rse and HPTK6 are used in the preparation and purification of antibodies thereto and in diagnostic assays.

What is claimed is:  
     
         1 . Isolated Rse or HPTK6 receptor protein tyrosine kinase (rPTK).  
     
     
         2 . The Rse rPTK of  claim 1 , wherein the Rse rPTK is antigenically active.  
     
     
         3 . The Rse rPTK of  claim 1 , wherein the Rse rPTK is biologically active.  
     
     
         4 . The Rse rPTK of  claim 1  sharing at least 80% sequence identity with the translated Rse sequence shown in FIG. 1A.  
     
     
         5 . The Rse rPTK of  claim 4  sharing at least 90% sequence identity with the translated Rse sequence shown in FIG. 1A.  
     
     
         6 . The HPTK6 rPTK of  claim 1 , wherein the HPTK6 rPTK is antigenically active.  
     
     
         7 . The HPTK6 rPTK of  claim 1 , wherein the HPTK6 rPTK is biologically active.  
     
     
         8 . The HPTK6 rPTK of  claim 1  sharing at least 80% sequence identity with the translated HPTK6 sequence shown in FIG. 2.  
     
     
         9 . The HPTK6 rPTK of  claim 8  sharing at least 90% sequence identity with the translated HPTK6 sequence shown in FIG. 2.  
     
     
         10 . An isolated receptor protein tyrosine kinase comprising an amino acid sequence selected from the group consisting of: 
 the amino acid sequence shown in FIG. 1A;    the amino acid sequence shown in FIG. 1B; and    the amino acid sequence shown in FIG. 2.    
     
     
         11 . An isolated extracellular domain of Rse receptor protein tyrosine kinase (rPTK) essentially free of transmembrane and intracellular domains of full sequence Rse rPTK or an isolated extracellular domain of HPTK6 rPTK essentially free of transmembrane and intracellular domains of full sequence HPTK6 rPTK.  
     
     
         12 . The extracellular domain of Rse rPTK of  claim 11 , sharing at least 80% sequence identity with the translated extracellular domain of Rse rPTK shown in FIG. 1A.  
     
     
         13 . The extracellular domain of Rse rPTK as claimed in  claim 12 , sharing at least 90% sequence identity with the translated extracellular domain of Rse rPTK shown in FIG. 1A.  
     
     
         14 . The extracellular domain of HPTK6 rPTK of  claim 11 , sharing at least 80% sequence identity with the translated extracellular domain of HPTK6 rPTK shown in FIG. 2.  
     
     
         15 . The extracellular domain of HPTK6 rPTK of  claim 14 , sharing at least 90% sequence identity with the translated extracellular domain of HPTK6 rPTK shown in FIG. 2.  
     
     
         16 . A composition comprising the Rse rPTK of  claim 3  and a pharmaceutically acceptable carrier.  
     
     
         17 . A composition comprising the HPTK6 rPTK of  claim 7  and a pharmaceutically acceptable carrier.  
     
     
         18 . An isolated ligand capable of binding Rse receptor protein tyrosine kinase (rPTK) or HPTK6 rPTK.  
     
     
         19 . The ligand of  claim 18 , wherein the ligand comprises a polyclonal antibody or a monoclonal antibody.  
     
     
         20 . An isolated Rse receptor protein tyrosine kinase (rPTK) or HPTK6 rPTK nucleic acid molecule.  
     
     
         21 . The isolated nucleic acid molecule of  claim 20  having a nucleic acid sequence selected from the group consisting of: 
 (a) the nucleic acid sequence shown in FIG. 1A;  
 (b) the nucleic acid sequence shown in FIG. 1B;  
 (c) the nucleic acid sequence shown in FIG. 2;  
 (d) a sequence corresponding to the sequence of (a), (b) or (c) within the scope of degeneracy of the genetic code;  
 (e) a sequence which hybridizes with a sequence complementary to the sequence from (a), (b), (c) or (d) under stringent conditions and which codes for a receptor protein with tyrosine kinase activity.  
 
     
     
         22 . An isolated nucleic acid molecule encoding a ligand to Rse receptor protein tyrosine kinase (rPTK) or HPTK6 rPTK.  
     
     
         23 . A vector comprising the nucleic acid molecule of  claim 20 .  
     
     
         24 . A host cell comprising the vector of  claim 23 .  
     
     
         25 . A method for preparing Rse receptor protein tyrosine kinase (rPTK) or HPTK6 rPTK comprising culturing a host cell transfected to express Rse rPTK or HPTK6 rPTK nucleic acid and recovering the Rse rPTK or HPTK6 rPTK from the host cell culture.