Antisense modulation of estrogen receptor alpha expression

Abstract

Antisense compounds, compositions and methods are provided for modulating the expression of estrogen receptor alpha. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding estrogen receptor alpha. Methods of using these compounds for modulation of estrogen receptor alpha expression and for treatment of diseases associated with expression of estrogen receptor alpha are provided.

What is claimed is:  
     
         1 . A compound 8 to 50 nucleobases in length targeted to a nucleic acid molecule encoding estrogen receptor alpha, wherein said compound specifically hybridizes with said nucleic acid molecule encoding estrogen receptor alpha and inhibits the expression of estrogen receptor alpha.  
     
     
         2 . The compound of  claim 1  which is an antisense oligonucleotide.  
     
     
         3 . The compound of  claim 2  wherein the antisense oligonucleotide has a sequence comprising SEQ ID NO: 19, 20, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 34, 35, 37, 39, 40, 42, 43, 44, 45, 46, 47, 48, 49, 50, 52, 53, 54, 56, 60, 74, 77, 88, 91 or 95.  
     
     
         4 . The compound of  claim 2  wherein the antisense oligonucleotide comprises at least one modified internucleoside linkage.  
     
     
         5 . The compound of  claim 4  wherein the modified internucleoside linkage is a phosphorothioate linkage.  
     
     
         6 . The compound of  claim 2  wherein the antisense oligonucleotide comprises at least one modified sugar moiety.  
     
     
         7 . The compound of  claim 6  wherein the modified sugar moiety is a 2′-O-methoxyethyl sugar moiety.  
     
     
         8 . The compound of  claim 2  wherein the antisense oligonucleotide comprises at least one modified nucleobase.  
     
     
         9 . The compound of  claim 8  wherein the modified nucleobase is a 5-methylcytosine.  
     
     
         10 . The compound of  claim 2  wherein the antisense oligonucleotide is a chimeric oligonucleotide.  
     
     
         11 . A compound 8 to 50 nucleobases in length which specifically hybridizes with at least an 8-nucleobase portion of an active site on a nucleic acid molecule encoding estrogen receptor alpha.  
     
     
         12 . A composition comprising the compound of  claim 1  and a pharmaceutically acceptable carrier or diluent.  
     
     
         13 . The composition of  claim 12  further comprising a colloidal dispersion system.  
     
     
         14 . The composition of  claim 12  wherein the compound is an antisense oligonucleotide.  
     
     
         15 . A method of inhibiting the expression of estrogen receptor alpha in cells or tissues comprising contacting said cells or tissues with the compound of  claim 1  so that expression of estrogen receptor alpha is inhibited.  
     
     
         16 . A method of treating an animal having a disease or condition associated with estrogen receptor alpha comprising administering to said animal a therapeutically or prophylactically effective amount of the compound of  claim 1  so that expression of estrogen receptor alpha is inhibited.  
     
     
         17 . The method of  claim 16  wherein the disease or condition is a hyperproliferative disease.  
     
     
         18 . The compound of  claim 1  targeted to a nucleic acid molecule encoding an estrogen receptor alpha/fatty acid synthase fusion transcript, wherein said compound specifically hybridizes with and differentially inhibits the expression of said fusion transcript relative to variants of estrogen receptor alpha.  
     
     
         19 . The compound of  claim 1  targeted to a nucleic acid molecule encoding estrogen receptor alpha, wherein said compound specifically hybridizes with and differentially inhibits the expression of one of the variants of estrogen receptor alpha relative to the remaining variants of estrogen receptor alpha.  
     
     
         20 . The compound of  claim 19  targeted to a nucleic acid molecule encoding estrogen receptor alpha, wherein said compound hybridizes with and specifically inhibits the expression of a variant of estrogen receptor alpha, wherein said variant is selected from the group consisting of ESR-alpha, ESR-alpha-II, ESR-alpha-III, ESR-alpha-IV, ESR-alpha-V, ESR-alpha-VI, ESR-alpha-VII, ESR-alpha-VIII and ESR-alpha-IX.