US2003221221A1PendingUtilityA1
Plants with modified growth
Est. expiryMar 26, 2017(expired)· nominal 20-yr term from priority
Inventors:James A. Murray
C12N 15/8261C12N 15/827C07K 14/415Y02A40/146
56
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Abstract
A process is provided for modifying growth or architecture of plants by altering the level or the functional level of a cell division controlling protein, preferably a cell-division controlling protein that binds or phosphorylates retinoblasoma-like proteins, more preferably a cyclin, particularly a D-type cyclin within cells of a plant. Also provided are chimeric genes comprising a transcribed DNA region encoding an RNA or a protein, which when expressed either increases or decreases the level or functional level of a cell-division controlling protein, and plant cells and plants expressing such chimeric genes.
Claims
exact text as granted — not AI-modified1 . A process to obtain a plant which, when compared to a wild type plant, exhibits a phenotype selected from increased growth rate, increased number of flowers or increased seed yield per plant, said process comprising the step of transforming cells of said plant with a chimeric gene comprising the following operably linked DNA fragments:
a) a plant expressible promoter region; b) a transcribed DNA region encoding a D-type cyclin, said D-type cyclin comprising the amino acid sequence of SEQ ID No. 30; and c) a 3′ end formation and polyadenylation signal functional in plant cells.
2 . The process of claim 1 , wherein said transcribed DNA region comprises a nucleotide sequence of SEQ ID No. 29 from the nucleotide position 195 to the nucleotide position 1280.
3 . The process of claim 1 , wherein said plant expressible promoter region is a CaMV35S promoter region.
4 . A chimeric gene comprising the following operably linked DNA fragments:
a) a plant expressible promoter region; b) a transcribed DNA region encoding a D-type cyclin, said D-type cyclin comprising the amino acid sequence of SEQ ID No. 30; and c) a 3′ end formation and polyadenylation signal functional in plant cells.
5 . A plant cell comprising the chimeric gene of claim 4 .
6 . A plant, consisting essentially of the plant cells of claim 5 .
7 . The plant of claim 6 , which is a greenhouse-grown plant.
8 . A seed of the plant of claim 7 , said seed comprising the chimeric gene of claim 4.Cited by (0)
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