Secreted proteins and polynucleotides encoding them

Abstract

Novel polynucleotides and the proteins encoded thereby are disclosed.

What is claimed is:  
     
         1 . An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: 
 (a) the nucleotide sequence of SEQ ID NO:41;    (b) the nucleotide sequence of SEQ ID NO:41 from nucleotide 161 to nucleotide 1348;    (c) the nucleotide sequence of SEQ ID NO:41 from nucleotide 599 to nucleotide 1348;    (d) the nucleotide sequence of the full-length protein coding sequence of clone dd504 — 18 deposited under accession number ATCC 98850;    (e) a nucleotide sequence encoding the full-length protein encoded by the cDNA insert of clone dd504 — 18 deposited under accession number ATCC 98850;    (f) the nucleotide sequence of a mature protein coding sequence of clone dd504 — 18 deposited under accession number ATCC 98850;    (g) a nucleotide sequence encoding a mature protein encoded by the cDNA insert of clone dd504 — 18 deposited under accession number ATCC 98850;    (h) a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:42;    (i) a nucleotide sequence encoding a protein comprising a fragment of the amino acid sequence of SEQ ID NO:42, the fragment comprising eight contiguous amino acids of SEQ ID NO:42;    (j) the nucleotide sequence of a polynucleotide that hybridizes under conditions at least as stringent as 4×SSC at 65 degrees C., or 4×SSC at 42 degrees C. with 50% formamide, to any one of the polynucleotides specified by (a)-(g); and    (k) the nucleotide sequence of a polynucleotide that hybridizes under conditions at least as stringent as 4×SSC at 50 degrees C., or 6×SSC at 40 degrees C. with 50% formamide, to any one of the polynucleotides specified by (a)-(g), and that has a length that is at least 25% of the length of SEQ ID NO:41.    
     
     
         2 . The polynucleotide of  claim 1  wherein said polynucleotide is operably linked to at least one expression control sequence.  
     
     
         3 . A host cell transformed with the polynucleotide of  claim 2 .  
     
     
         4 . The host cell of  claim 3 , wherein said cell is a mammalian cell.  
     
     
         5 . A process for producing a protein encoded by the polynucleotide of  claim 2 , which process comprises: 
 (a) growing a culture of a host cell in a suitable culture medium wherein the host cell has been transformed with the polynucleotide of  claim 2;  and    (b) purifying said protein from the culture.    
     
     
         6 . A protein produced according to the process of  claim 5 .  
     
     
         7 . An isolated polynucleotide encoding the protein of  claim 6 .  
     
     
         8 . The polynucleotide of  claim 7 , wherein the polynucleotide comprises the cDNA insert of clone dd504 — 18 deposited under accession number ATCC 98850.  
     
     
         9 . A protein comprising an amino acid sequence selected from the group consisting of: 
 (a) the amino acid sequence of SEQ ID NO:42;    (b) a fragment of the amino acid sequence of SEQ ID NO:42, the fragment comprising eight contiguous amino acids of SEQ ID NO:42; and    (c) the amino acid sequence encoded by the cDNA insert of clone dd504 — 18 deposited under accession number ATCC 98850;    the protein being substantially free from other mammalian proteins.    
     
     
         10 . The protein of  claim 9 , wherein said protein comprises the amino acid sequence of SEQ ID NO:42.  
     
     
         11 . A composition comprising the protein of  claim 9  and a pharmaceutically acceptable carrier.  
     
     
         12 . An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: 
 (a) the nucleotide sequence of SEQ ID NO:51;    (b) the nucleotide sequence of SEQ ID NO:51 from nucleotide 379 to nucleotide 3783;    (c) the nucleotide sequence of SEQ. ID NO:51 from nucleotide 460 to nucleotide 3783;    (d) the nucleotide sequence of SEQ ID NO:51 from nucleotide 1983 to nucleotide 3938,    (e) the nucleotide sequence of the full-length protein coding sequence of clone qs14 — 3 deposited under accession number ATCC 98850;    (f) a nucleotide sequence encoding the full-length protein encoded by the cDNA insert of clone qs14 — 3 deposited under accession number ATCC 98850;    (g) the nucleotide sequence of a mature protein coding sequence of clone qs14 — 3 deposited under accession number ATCC 98850;    (h) a nucleotide sequence encoding a mature protein encoded by the cDNA insert of clone qs14 — 3 deposited under accession number ATCC 98850;    (i) a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:52;    (j) a nucleotide sequence encoding a protein comprising a fragment of the amino acid sequence of SEQ ID NO:52, the fragment comprising eight contiguous amino acids of SEQ ID NO:52;    (k) the nucleotide sequence of a polynucleotide that hybridizes under conditions at least as stringent as 4×SSC at 65 degrees C., or 4×SSC at 42 degrees C. with 50% formamide, to any one of the polynucleotides specified by (a)-(h); and    (l) the nucleotide sequence of a polynucleotide that hybridizes under conditions at least as stringent as 4×SSC at 50 degrees C., or 6×SSC at 40 degrees C with 50% formamide, to any one of the polynucleotides specified by (a)-(h), and that has a length that is at least 25% of the length of SEQ ID NO:51.    
     
     
         13 . A protein comprising an amino acid sequence selected from the group consisting of: 
 (a) the amino acid sequence of SEQ ID NO:52;    (b) the amino acid sequence of SEQ ID NO:52 from amino acid 536 to amino acid 1135;    (c) a fragment of the amino acid sequence of SEQ ID NO:52, the fragment comprising eight contiguous amino acids of SEQ ID NO:52; and    (d) the amino acid sequence encoded by the cDNA insert of clone qs14 — 3 deposited under accession number ATCC 98850;    the protein being substantially free from other mammalian proteins.