Novel polypeptides, cDNA encoding the same and utilization thereof

Abstract

Structure A novel human polypeptide, the method for prepareation thereof, a cDNA encoding the polypeptide; a fragment selectively hybridizing with the sequence of the cDNA, a replication or expression plasmid containing the cDNA integrated thereinto, a host cell transformed with plasmid, an antibody against the polypeptide and a pharmaceutical composition containing the polypeptide or the antibody. Effect The polypeptides of the present invention possess hematopoiesis regulating activity, tissue generation/regeneration activity, activin/inhibin activity, chemotactic/chemokinetic activity, hemostatic and thrombolytic activity, and receptor/ligand activity, therefore, they are expected to be useful for prevention and/or treatment of various diseases.

1 . A substantially purified form of the polypeptide comprising the amino acid sequence shown in SEQ ID NOS. 1, 4, 6, 9 or 12, homologue thereof, fragment thereof or homologue of the fragment.  
     
     
         2 . A polypeptide according to  claim 1  comprising the amino acid sequence shown in SEQ ID NOS. 1, 4, 6, 9 or 12.  
     
     
         3 . A cDNA encoding the polypeptide according to  claim 1 .  
     
     
         4 . A cDNA according to  claim 3  comprising the nucleotide sequence shown in SEQ ID NOS. 2, 5, 7, 10 or 13, or a fragment cDNA selectively hybridized to the cDNA.  
     
     
         5 . A cDNA according to  claim 3  comprising the nucleotide sequence shown in SEQ ID NOS. 3, 8, 11 or 14, or a fragment cDNA selectively hybridized to the cDNA.  
     
     
         6 . A replication or expression vector carrying the cDNA according to  claims 3  to  5 .  
     
     
         7 . A host cell transformed with the replication or expression vector according to  claim 6 .  
     
     
         8 . A method for producing the polypeptide according to  claim 1  or  2  which comprises culturing a host cell according to  claim 7  under a condition effective to express the polypeptide according to  claim 1  or  2 .  
     
     
         9 . A monoclonal or polyclonal antibody against the polypeptide according to  claim 1  or  2 .  
     
     
         10 . A pharmaceutical composition containing the polypeptide according to  claim 1  or  2  or the antibody according to  claim 9 , in association with pharmaceutically acceptable diluent and/or carrier.