Prostaglandin D synthase-specific monoclonal antibody

Abstract

The invention relates to a monoclonal antibody specifically recognizing lipocalin-type prostaglandin D synthase (L-PGDS), a hybridoma producing said monoclonal antibody, methods for detection of L-PGDS or diseases by said monoclonal antibody, and a kit for detection of L-PGDS by said monoclonal antibody. According to the invention, there is provided a monoclonal antibody specific to L-PGDS.

1 . A monoclonal antibody specifically recognizing lipocalin-type prostaglandin D synthase (L-PGDS).  
     
     
         2 . The monoclonal antibody according to  claim 1 , wherein the subclass of said monoclonal antibody is immunoglobulin G1 or G2.  
     
     
         3 . A hybridoma obtained by cell fusion between antibody-producing cells from an animal immunized with L-PGDS and myeloma cells and producing a monoclonal antibody of  claim 1  or  2 .  
     
     
         4 . A method for detection of L-PGDS, wherein L-PGDS is detected by a monoclonal antibody of  claim 1  or  2 .  
     
     
         5 . A method for detection of a disease, wherein L-PGDS is detected by a monoclonal antibody of  claim 1  or  2 .  
     
     
         6 . The method according to  claim 5 , wherein the disease is oligospermia.  
     
     
         7 . A kit for detection of L-PGDS, which is selected from the group consisting of: 
 a reagent containing the monoclonal antibody of  claim 1  or  2  labeled with an enzyme, and a substrate solution;    a reagent containing the monoclonal antibody of  claim 1  or  2 , said monoclonal antibody being labeled with an enzyme, or an enzyme-labeled polyclonal antibody recognizing human L-PGDS, and a substrate solution;    a reagent containing the monoclonal antibody of  claim 1  or  2  which is biotinylated, enzyme-labeled avidin or streptoavidin, and a substrate solution; and    a reagent containing the monoclonal antibody of  claim 1  or  2 , said monoclonal antibody being biotinylated, or a biotinylated polyclonal antibody recognizing human L-PGDS, enzyme-labeled avidin or streptoavidin, and a substrate solution.    
     
     
         8 . A method for detection of a disease, wherein the disease is detected using the kit for detection of L-PGDS described in  claim 7 .  
     
     
         9 . The method according to  claim 8 , wherein the disease is oligospermia.