Staphylococcus aureus polynucleotides and sequences

Abstract

The present invention provides polynucleotide sequences of the genome of Staphylococcus aureus, polypeptide sequences encoded by the polynucleotide sequences, corresponding polynucleotides and polypeptides, vectors and hosts comprising the polynucleotides, and assays and other uses thereof. The present invention further provides polynucleotide and polypeptide sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use.

What is claimed is:  
     
         1 . An isolated polynucleotide comprising a nucleic acid fragment of the  Staphylococcus aureus  genome, wherein said fragment consists of the nucleotide sequence of any one of SEQ ID NOS: 1-5,191 as depicted in Tables 2 and 3.  
     
     
         2 . A vector comprising the polynucleotide of  claim 1 .  
     
     
         3 . An organism which has been altered to contain the polynucleotide of  claim 1 .  
     
     
         4 . An isolated polypeptide encoded by any one of the fragments of the  Staphylococcus aureus  genome of SEQ ID NOS: 1-5,191 and depicted in Tables 2 and 3.  
     
     
         5 . An antibody which specifically binds the polypeptide of  claim 4 .  
     
     
         6 . An isolated polynucleotide encoding the polypeptide of  claim 4 .  
     
     
         7 . A vector comprising the polynucleotide of  claim 6 .  
     
     
         8 . An organism which has been altered to contain the polynucleotide of  claim 6 .  
     
     
         9 . A method for producing a polypeptide in a host cell comprising the steps of: 
 (a) incubating a host containing a heterologous nucleic acid molecule whose nucleotide sequence consists of the polynucleotide of  claim 6  under conditions where said heterologous nucleic acid molecule is expressed to produce said polypeptide, and    (b) isolating said polypeptide.    
     
     
         10 . An isolated polypeptide comprising an amino acid sequence identical to a  Staphylococcus aureus  polypeptide amino acid sequence selected from the group consisting of SEQ ID NOS: 5,192 to 5,255.