Delivery of disease control in aquaculture and agriculture using microbes containing bioactive proteins

Abstract

A microbial biomass, made from algae, bacteria, fungi, yeast, or combinations thereof, provides a feed for animals raised either in agriculture or aquaculture. A feed additive, and a therapeutic composition can also be made from a microbial biomass of algae, bacteria, fungi, yeast, or combinations thereof. The feed, feed additive, and therapeutic composition can comprise one or more proteins, peptides, antibodies, antibody fragments, or a combination thereof, wherein said proteins, peptides, antibodies, antibody fragments, or a combination thereof are non-native to the microbes of the biomass. The biomass can have therapeutic, bioactive, nutritional, and/or immunogenic properties.

1 . A composition comprising (1) an algal biomass or any parts thereof, wherein the algal biomass is chosen from eukaryotic algae or prokaryotic algae sources, and (2) one or more proteins, peptides, antibodies, antibody fragments, or combination thereof, which are non-native to the algae of the biomass.  
     
     
         2 . The composition of  claim 1 , wherein the composition is a feed.  
     
     
         3 . The composition of  claim 1 , wherein the composition is a feed additive.  
     
     
         4 . The composition of  claim 1 , wherein the composition is therapeutic.  
     
     
         5 . The composition of  claim 1 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof is expressed recombinantly.  
     
     
         6 . The composition of  claim 5 , further comprising a recombinant virus that expresses the protein, peptide, antibody, antibody fragment, or combination thereof.  
     
     
         7 . The composition of  claim 1 , wherein the composition inhibits the growth or replication of a pathogen.  
     
     
         8 . The composition of  claim 7 , wherein the pathogen is Vibrio.  
     
     
         9 . The composition of  claim 7 , wherein the pathogen is chosen from Taura Syndrome Virus, White Spot Syndrome Virus, and Infectious Pancreatic Necrosis Virus.  
     
     
         10 . The composition of  claim 1 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof is bactericidal or bacteriostatic.  
     
     
         11 . The composition of  claim 10 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof is chosen from cecropins, penaeidins, bactenecins, callinectins, myticins, tachyplesins, clavanins, misgurins, pleurocidins, parasins, histones, acidic proteins, and lysozymes.  
     
     
         12 . The composition of  claim 1 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof comprises an immunogenic epitope.  
     
     
         13 . The composition of  claim 1 , wherein the algal biomass comprises heterotrophic microalgae.  
     
     
         14 . The composition of  claim 1 , wherein the algal biomass comprises photosynthetic microalgae.  
     
     
         15 . The composition of  claim 1  wherein the algae are chosen from Synechocystis and Chlorella strains.  
     
     
         16 . The composition of  claim 1 , wherein the algae are probiotic.  
     
     
         17 . A method of feeding an animal comprising administering to said animal a composition comprising (1) an algal biomass or any parts thereof, wherein the algal biomass is chosen from eukaryotic algae or prokaryotic algae sources, and (2) one or more proteins, peptides, antibodies, antibody fragments, or combination thereof, which are non-native to an organism of the algal biomass.  
     
     
         18 . The method of  claim 17 , wherein the composition is a feed.  
     
     
         19 . The method of  claim 17 , wherein the composition is a feed additive.  
     
     
         20 . The method of  claim 17 , wherein the composition is therapeutic.  
     
     
         21 . The method of  claim 17 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof confers passive immunity upon the animal.  
     
     
         22 . The method of  claim 17 , wherein the animal is raised in aquaculture.  
     
     
         23 . The method of  claim 2 , wherein the animal is chosen from a fish and a crustacean.  
     
     
         24 . The method of  claim 23 , wherein the animal is chosen from a salmon and a shrimp.  
     
     
         25 . The method of  claim 17 , wherein the animal is raised in agriculture.  
     
     
         26 . The method of  claim 25 , wherein the animal is chosen from cattle, pigs, and fowl.  
     
     
         27 . The method of  claim 17 , wherein the animal is a human.  
     
     
         28 . A composition comprising (1) a yeast biomass or any parts thereof, and (2) one or more proteins, peptides, antibodies, antibody fragments, or combination thereof which are non-native to the yeast of the biomass.  
     
     
         29 . The composition of  claim 28 , wherein the composition is a feed.  
     
     
         30 . The composition of  claim 28 , wherein the composition is a feed additive.  
     
     
         31 . The composition of  claim 28 , wherein the composition is therapeutic.  
     
     
         32 . The composition of  claim 28 , wherein the yeast biomass comprises a recombinant virus that expresses the protein, peptide, antibody, antibody fragment, or combination thereof.  
     
     
         33 . The composition of  claim 28 , wherein the composition inhibits the growth or replication of a pathogen.  
     
     
         34 . The composition of  claim 33 , wherein the pathogen is Vibrio.  
     
     
         35 . The composition of  claim 33 , wherein the pathogen is chosen from Taura Syndrome Virus, White Spot Syndrome Virus, and Infectious Pancreatic Necrosis Virus.  
     
     
         36 . The composition of  claim 28 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof is bactericidal or bacteriostatic.  
     
     
         37 . The composition of  claim 36 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof is chosen from cecropins, penaeidins, bactenecins, callinectins, myticins, tachyplesins, clavanins, misgurins, pleurocidins, parasins, histones, acidic proteins, and lysozymes.  
     
     
         38 . The composition of  claim 28 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof comprises an immunogenic epitope.  
     
     
         39 . The composition of  claim 28 , wherein the yeast is a Saccharomyces strain.  
     
     
         40 . The composition of  claim 28 , wherein the yeast are probiotic.  
     
     
         41 . A method of feeding an animal comprising administering to said animal a composition comprising (1) a yeast biomass or any parts thereof, and (2) one or more proteins, peptides, antibodies, antibody fragments, or combination thereof, which are non-native to the organism of the yeast biomass.  
     
     
         42 . The method of  claim 41 , wherein the composition is a feed.  
     
     
         43 . The method of  claim 41 , wherein the composition is a feed additive.  
     
     
         44 . The method of  claim 41 , wherein the composition is therapeutic.  
     
     
         45 . The method of  claim 41 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof confers passive immunity upon the animal.  
     
     
         46 . The method of  claim 41 , wherein the animal is raised in aquaculture.  
     
     
         47 . The method of  claim 46 , wherein the animal is chosen from a fish and a crustacean.  
     
     
         48 . The method of  claim 47 , wherein the animal is chosen from a salmon and a shrimp.  
     
     
         49 . The method of  claim 41 , wherein the animal is raised in agriculture.  
     
     
         50 . The method of  claim 49 , wherein the animal is chosen from cattle, pigs, and fowl.  
     
     
         51 . The method of  claim 41 , wherein the animal is a human.  
     
     
         52 . A composition comprising (1) a fungal biomass or any parts thereof, and (2) one or more proteins, peptides, antibodies, antibody fragments, or combination thereof, which are non-native to the fungi of the biomass.  
     
     
         53 . The composition of  claim 52 , wherein the composition is a feed.  
     
     
         54 . The composition of  claim 52 , wherein the composition is a feed additive.  
     
     
         55 . The composition of  claim 52 , wherein the composition is therapeutic.  
     
     
         56 . The composition of  claim 52 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof is expressed recombinantly.  
     
     
         57 . The composition of  claim 56 , further comprising a recombinant virus that expresses the protein, peptide, antibody, antibody fragment, or combination thereof.  
     
     
         58 . The composition of  claim 52 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof inhibits the growth or replication of a pathogen.  
     
     
         59 . The composition of  claim 58 , wherein the pathogen is chosen from Vibrio, Taura Syndrome Virus, White Spot Syndrome Virus, and Infectious Pancreatic Necrosis Virus.  
     
     
         60 . The composition of  claim 52 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof, is bactericidal or bacteriostatic.  
     
     
         61 . The composition of  claim 60 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof, is chosen from cecropins, penaeidins, bactenecins, callinectins, myticins, tachyplesins, clavanins, misgurins, pleurocidins, parasins, histones, acidic proteins, and lysozymes.  
     
     
         62 . The composition of  claim 52 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof comprises an immunogenic epitope.  
     
     
         63 . The composition of  claim 52 , wherein the fungus is a Mortierella species.  
     
     
         64 . The composition of  claim 52 , wherein the fungal biomass or any parts thereof is probiotic.  
     
     
         65 . A method of feeding an animal comprising administering to said animal a composition comprising (1) a fungal biomass or any parts thereof, and (2) one or more proteins, peptides, antibodies, antibody fragments, or combination thereof, which are non-native to the fungi of the biomass.  
     
     
         66 . The method of  claim 65 , wherein the composition is a feed.  
     
     
         67 . The method of  claim 65 , wherein the composition is a feed additive.  
     
     
         68 . The method of  claim 65 , wherein the composition is therapeutic.  
     
     
         69 . The method of  claim 65 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof confers passive immunity upon the animal.  
     
     
         70 . The method of  claim 65 , wherein the animal is raised in aquaculture.  
     
     
         71 . The method of  claim 70 , wherein the animal is chosen from a fish and a crustacean.  
     
     
         72 . The method of  claim 71 , wherein the animal is chosen from a salmon and a shrimp.  
     
     
         73 . The method of  claim 65 , wherein the animal is raised in agriculture.  
     
     
         74 . The method of  claim 73 , wherein the animal is chosen from cattle, pigs, and fowl.  
     
     
         75 . The method of  claim 65 , wherein the animal is a human.  
     
     
         76 . A composition comprising (1) a bacterial biomass or any parts thereof, (2) one or more proteins, peptides, antibodies, antibody fragments, or combination thereof, which are non-native to the bacteria of the biomass, and (3) a recombinant virus that expresses the protein, peptide, antibody, antibody fragment, or combination thereof.  
     
     
         77 . The composition of  claim 76 , wherein the composition is a feed.  
     
     
         78 . The composition of  claim 76 , wherein the composition is a feed additive.  
     
     
         79 . The composition of  claim 76 , wherein the composition is therapeutic.  
     
     
         80 . The composition of  claim 76 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof, inhibits the growth or replication of a pathogen.  
     
     
         81 . The composition of  claim 80 , wherein the pathogen is chosen from Vibrio, Taura Syndrome Virus, White Spot Syndrome Virus, and Infectious Pancreatic Necrosis Virus.  
     
     
         82 . The composition of  claim 76 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof is bactericidal or bacteriostatic.  
     
     
         83 . The composition of  claim 82 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof is chosen from cecropins, penaeidins, bactenecins, callinectins, myticins, tachyplesins, clavanins, misgurins, pleurocidins, parasins, histones, acidic proteins, and lysozymes.  
     
     
         84 . The composition of  claim 76 , wherein the protein, peptide, antibody, antibody fragment, or combination thereof comprises an immunogenic epitope.  
     
     
         85 . The composition of  claim 52 , wherein the fungal biomass or any parts thereof is probiotic.  
     
     
         86 . A method of feeding an animal comprising administering to said animal a composition comprising (1) a bacterial biomass or any parts thereof, and (2) one or more proteins, peptides, antibodies, antibody fragments, or combination thereof, which are non-native to an organism of the bacterial of the biomass, and (3) a recombinant virus that expresses the protein, peptide, antibody, antibody fragment, or combination thereof.  
     
     
         87 . The method of  claim 86 , wherein the composition is a feed.  
     
     
         88 . The method of  claim 86 , wherein the composition is a feed additive.  
     
     
         89 . The method of  claim 86 , wherein the composition is therapeutic.  
     
     
         90 . The method of  claim 86 , wherein the polypeptide confers passive immunity upon the animal.  
     
     
         91 . The method of  claim 86 , wherein the animal is raised in aquaculture.  
     
     
         92 . The method of  claim 96 , wherein the animal is chosen from a fish and a crustacean.  
     
     
         93 . The method of  claim 92 , wherein the animal is chosen from a salmon and a shrimp.  
     
     
         94 . The method of  claim 86 , wherein the animal is raised in agriculture.  
     
     
         95 . The method of  claim 94 , wherein the animal is chosen from cattle, pigs, and fowl.  
     
     
         96 . The method of  claim 86 , wherein the animal is a human.  
     
     
         97 . The method of  claim 86 , wherein the composition expresses the protein, peptide, antibody, antibody fragment, or combination thereof.