Treatment of skin with adenosine or adenosine analog

Abstract

Methods for enhancing the condition of non-diseased skin by application of compositions containing adenosine or an adenosine analog are disclosed. Also disclosed are methods for increasing DNA synthesis or protein synthesis in dermal cells, and methods for increasing dermal cell size, by application of compositions containing adenosine.

We claim:  
     
         1 . A method for enhancing the condition of non-diseased skin of a mammal, comprising topically applying a therapeutically effective amount of a composition comprising adenosine or an adenosine agonist to non-diseased skin of said mammal.  
     
     
         2 . The method of  claim 1 , wherein said composition further comprises an angiogenic factor.  
     
     
         3 . The method of  claim 1 , wherein the therapeutically effective amount of adenosine is an adenosine concentration of 10 −3  M to 10 −7  M.  
     
     
         4 . The method of  claim 3 , wherein said adenosine concentration is 10 −4  M to 10 −6  M.  
     
     
         5 . The method of  claim 4 , wherein said adenosine concentration is about 10 −4  M.  
     
     
         6 . The method of  claim 1 , wherein said composition further comprises a conditioning agent.  
     
     
         7 . The method of  claim 6 , wherein said conditioning agent is selected from the group consisting of a humectant, an emollient, and occlusive agent.  
     
     
         8 . The method of  claim 1 , wherein addition of adenosine does not affect skin cell proliferation.  
     
     
         9 . The method of  claim 1 , wherein said skin comprises a skin graft.  
     
     
         10 . The method of  claim 1 , wherein said mammal is a human.  
     
     
         11 . A method for promoting healing of broken, non-diseased skin in a mammal, comprising topically administering a composition comprising a therapeutically effective amount of adenosine or an adenosine agonist to said mammal.  
     
     
         12 . The method of  claim 11 , wherein said composition further comprises an angiogenic factor.  
     
     
         13 . The method of  claim 11 , wherein the therapeutically effective amount of adenosine is an adenosine concentration of 10 −3  M to 10 −7  M.  
     
     
         14 . The method of  claim 13 , wherein said adenosine concentration is 10 −4  M to 10 −6  M.  
     
     
         15 . The method of  claim 14 , wherein said adenosine concentration is about 1×10 −4  M.  
     
     
         16 . The method of  claim 11 , wherein said composition further comprises a conditioning agent.  
     
     
         17 . The method of  claim 16 , wherein said conditioning agent is selected from the group consisting of a humectant, an emollient, and occlusive agent.  
     
     
         18 . The method of  claim 11 , wherein addition of adenosine does not affect skin cell proliferation.  
     
     
         19 . The method of  claim 11 , wherein said region of skin comprises a skin graft.  
     
     
         20 . The method of  claim 11 , wherein said mammal is a human.  
     
     
         21 . A method for increasing DNA synthesis in a dermal cell of non-diseased skin of a mammal, comprising topically administering a therapeutically effective amount of adenosine to a region of non-diseased skin of said mammal containing said dermal cell, wherein addition of said adenosine does not cause proliferation of said dermal cell.  
     
     
         22 . The method of  claim 21 , wherein said composition further comprises an angiogenic factor.  
     
     
         23 . The method of  claim 21 , wherein the therapeutically effective amount of adenosine is an adenosine concentration of 10 −3  M to 10 −7  M.  
     
     
         24 . The method of  claim 23 , wherein said adenosine concentration is 10 −4  M to 10 −6  M.  
     
     
         25 . The method of  claim 24 , wherein said adenosine concentration is about 10 −4  M.  
     
     
         26 . The method of  claim 21 , wherein said composition further comprises a conditioning agent.  
     
     
         27 . The method of  claim 26 , wherein said conditioning agent is selected from the group consisting of a humectant, an emollient, and occlusive agent.  
     
     
         28 . The method of  claim 21 , wherein said region of skin comprises a skin graft.  
     
     
         29 . The method of  claim 21 , wherein said mammal is a human.  
     
     
         30 . A method of increasing protein synthesis in a dermal cell of non-diseased skin of a mammal, comprising topically administering a composition comprising a therapeutically effective amount of adenosine to a region of skin of said mammal containing said dermal cell, wherein addition of said adenosine does not cause proliferation of said dermal cell.  
     
     
         31 . The method of  claim 30 , wherein said composition further comprises an angiogenic factor.  
     
     
         32 . The method of  claim 30 , wherein the therapeutically effective amount of adenosine is an adenosine concentration of 10 −3  M to  10   −7  M.  
     
     
         33 . The method of  claim 32 , wherein said adenosine concentration is 10 −4  M to  10   −6  M.  
     
     
         34 . The method of  claim 33 , wherein said adenosine concentration is about 10 −4  M.  
     
     
         35 . The method of  claim 30 , wherein said composition further comprises a conditioning agent.  
     
     
         36 . The method of  claim 35 , wherein said conditioning agent is selected from the group consisting of a humectant, an emollient, and occlusive agent.  
     
     
         37 . The method of  claim 30 , wherein said region of skin comprises a skin graft.  
     
     
         38 . The method of  claim 30 , wherein said mammal is a human.  
     
     
         39 . A method of increasing cell size in a dermal cell in non-diseased skin of a mammal, comprising topically administering a composition comprising a therapeutically effective amount of adenosine to a region of skin of said mammal containing said dermal cell, wherein addition of said adenosine does not cause proliferation of said dermal cell, wherein addition of said adenosine does not cause proliferation of said dermal cell.  
     
     
         40 . The method of  claim 39 , wherein said composition further comprises an angiogenic factor.  
     
     
         41 . The method of  claim 39 , wherein the therapeutically effective amount of adenosine is an adenosine concentration of 10 −3  M to 10 −7  M.  
     
     
         42 . The method of  claim 41 , wherein said adenosine concentration is 10 −4  M to 10 −6  M.  
     
     
         43 . The method of  claim 42 , wherein said adenosine concentration is about 10 −6  M.  
     
     
         44 . The method of  claim 39 , wherein said composition further comprises a conditioning agent.  
     
     
         45 . The method of  claim 44 , wherein said conditioning agent is selected from the group consisting of a humectant, an emollient, and occlusive agent.  
     
     
         46 . The method of  claim 39 , wherein said region of skin comprises a skin graft.  
     
     
         47 . The method of  claim 39 , wherein said mammal is a human.  
     
     
         48 . A method for enhancing skin condition in a mammal, comprising 
 providing fibroblasts from said mammal ex vivo,    culturing said fibroblasts in the presence of adenosine; and    reintroducing said fibroblasts into said mammal.    
     
     
         49 . The method of  claim 48 , wherein the adenosine concentration in said culturing step is from about 10 −3  M to about 10 −7  M.  
     
     
         50 . A method for increasing protein synthesis in a cultured skin fibroblast, comprising culturing said fibroblast in a culture medium comprising about 10 −3  M to about 10 −7  M adenosine.  
     
     
         51 . The method of  claim 50 , wherein the adenosine concentration is about 10 −4  M.  
     
     
         52 . A composition comprising 10 −3  M to 10 −7  M adenosine and an angiogenesis factor.  
     
     
         53 . The composition of  claim 52 , wherein the concentration of said adenosine is about 10 −4  M.