Recombinant preduodenal lipases and polypeptides derivatives produced by plants, processes for obtaining them and their uses

Abstract

The invention concerns the use of recombinant nucleotides sequences containing cDNA coding for a preduodenal lipase, or any sequence derived from this cDNA, for transforming plant cells in order to obtain recombinant preduodenal lipase or polypeptide derivatives. The invention also concerns the use of genetically modified plants or parts thereof, or extracts of these plants or the use of recombinant preduodenal lipase or resultant polypeptide derivatives in the field of foodstuffs, or for producing medicaments, or in industry.

1 . A recombinant dog gastric lipase comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 4 and 6.  
     
     
         2 . A recombinant dog gastric lipase comprising an amino acid sequence selected from the group of sequences consisting of: SEQ ID NOs: 4 and 6, wherein said amino acid sequence has its N-terminal fused to a signal peptide sequence or a prepropeptide sequence.  
     
     
         3 . The recombinant dog gastric lipase of  claim 2 , wherein said signal peptide sequence or said prepropeptide sequence is derived from a plant.  
     
     
         4 . The recombinant dog gastric lipase of  claim 3 , wherein said plant signal peptide sequence is encoded by a nucleotide sequence of SEQ ID NO: 14.  
     
     
         5 . The recombinant dog gastric lipase of  claim 3 , wherein said plant prepropeptide sequence is encoded by a nucleotide sequence of SEQ ID NO: 17.