Purified insulin composition

Abstract

The invention concerns a process of chromatographically separating glycosylated proteins from non-glycosylated proteins by subjecting a solution comprising glycolsylated and non-glycosylated proteins to chromatography using a Ca ++ containing eluant. By using this process a fraction comprising non-glycosylated proteins substantially free from glycosylated proteins is obtained. The process may be applied to the separation of proteins used in the medical industry, such as insulin.

1 . A composition comprising recombinant insulin or an analogue thereof, wherein (i) said composition comprises about 0.02% to 0.2% glycosylated insulin or insulin analogue and (ii) said recombinant insulin or insulin analogue is produced in a yeast cell.  
     
     
         2 . A composition as defined in  claim 1 , wherein said glycosylated insulin or insulin analogue is mono-glycosylated.