Culture medium with yeast or soy bean extract as amino acid source and no protein complexes of animal origin

Abstract

The invention relates to a medium for culturing pathogenic bacteria to produce an immunogenic factor. The medium comprises non-animal derived proteinaceous material. The invention also relates to the use of the medium to cultivate pathogenic bacteria, obtaining immunogenic factors from the bacteria being cultivated and preparing vaccines using the immunogenic factors.

1 . A medium for cultivating pathogenic bacteria to produce an immunogenic factor wherein the medium comprises at least 20% by dry weight of a non-animal derived proteinaceous material, and does not comprise animal derived proteinaceous material.  
     
     
         2 . The medium of  claim 1  wherein the non-animal derived proteinaceous material is a yeast extract and/or a soy bean derived protein composition.  
     
     
         3 . The medium of  claim 1  or  claim 2  wherein the pathogenic bacteria are  Helicobacter pylori, Haemophilus influenzae, Corynebacterium diphtheriae, Neisseria meningitidis, Bordetella pertussis  or  Clostridium tetani.    
     
     
         4 . A culture comprising the medium of any one of the previous claims and pathogenic bacteria.  
     
     
         5 . A process for preparing an immunogenic factor of a pathogenic bacteria comprising the steps of cultivating the bacteria in the medium of any one of  claims 1  to  3  and optionally purifying the immunogenic factor from the medium.  
     
     
         6 . A process for the production of a vaccine comprising preparing an immunogenic factor of a pathogenic bacteria by the process of  claim 5 , and bringing said factor, optionally toxoided, into association with a pharmaceutically acceptable carrier.  
     
     
         7 . A process for making a medium for cultivating pathogenic bacteria to produce an immunogenic factor comprising adding sufficient non-animal derived proteinaceous material to a standard medium for cultivating bacteria, which does not comprise animal derived proteinaceous material, so that the medium for cultivating pathogenic bacteria comprises at least 20% by dry weight of the non-animal derived proteinaceous material, and does not comprise animal derived proteinaceous material.