Methods and compositions for enhancing fibroblast migration

Abstract

Methods and compositions for enhancing fibroblast migration at a wound site are disclosed. The method includes contacting the wound site with fibrinogen that is prepared by a process which includes precipitating plasma with glycine. The compositions includes a lipid rich component and fibrinogen.

What is claimed:  
     
         1 . A method for enhancing fibroblast migration at a wound site comprising: 
 contacting the wound site with a fibrinogen preparation, wherein the fibrinogen preparation includes a lipid rich component.    
     
     
         2 . A method according to  claim 1  wherein the fibrinogen preparation further comprises fibrinogen prepared by a process which comprises precipitating plasma with glycine.  
     
     
         3 . A method according to  claim 2  wherein the fibrinogen preparation further comprises a growth factor, an extracellular matrix material, or mixtures thereof.  
     
     
         4 . A method according to  claim 2  wherein the precipitating is carried out by a process which comprises: 
 adding glycine to plasma to produce a precipitate and a supernatant;  
 dissolving the precipitate in a buffer to produce a solution; and  
 precipitating the solution by adding glycine to the solution.  
 
     
     
         5 . A method according to  claim 2  wherein the fibrinogen in prepared by a process comprising: 
 precipitating plasma with glycine to produce a first precipitate and a first supernatant;  
 dissolving the first precipitate in a buffer to produce a first solution;  
 precipitating the first solution by adding glycine to the first solution to produce a second precipitate and a second supernatant;  
 dissolving the second precipitate in a buffer to produce a second solution; and  
 precipitating the second solution by adding ammonium sulfate to the second solution to produce a third precipitate and a third supernatant.  
 
     
     
         6 . A method according to  claim 5  wherein the third supernatant comprises a lipid rich layer.  
     
     
         7 . A method according to  claim 6  wherein the third supernatant is further treated to produce the lipid rich component.  
     
     
         8 . A method according to  claim 7  wherein the third supernatant is precipitated to produce the lipid rich component.  
     
     
         9 . A composition comprising: 
 a lipid rich component and    fibrinogen.    
     
     
         10 . A composition according to  claim 9  wherein the fibrinogen has a purity of above 95%.  
     
     
         11 . A composition according to  claim 9  wherein the fibrinogen has a purity of about 99%.  
     
     
         12 . A composition according to  claim 9  wherein the fibrinogen is prepared by a process which comprises precipitating plasma with glycine.  
     
     
         13 . A composition according to  claim 12  wherein the fibrinogen is prepared by a process which comprises: 
 precipitating plasma with glycine to produce a first precipitate and a first supernatant;  
 dissolving the first precipitate in a buffer to produce a first solution;  
 precipitating the first solution by adding glycine to the first solution to produce a second precipitate and a second supernatant;  
 dissolving the second precipitate in a buffer to produce a second solution; and  
 precipitating the second solution by adding ammonium sulfate to the second solution to produce a third precipitate and a third supernatant.  
 
     
     
         14 . A composition according to  claim 9  wherein the lipid rich component is prepared by a process which comprises precipitating plasma with glycine.  
     
     
         15 . A composition according to  claim 14  wherein the lipid rich component is prepared by a process which comprises: 
 precipitating plasma with glycine to produce a first precipitate and a first supernatant;  
 dissolving the first precipitate in a buffer to produce a first solution;  
 precipitating the first solution by adding glycine to the first solution to produce a second precipitate and a second supernatant;  
 dissolving the second precipitate in a buffer to produce a second solution;  
 precipitating the second solution by adding ammonium sulfate to the second solution to produce a third precipitate and a third supernatant; and  
 precipitating the third supernatant to produce the lipid rich component.