US2004147492A1PendingUtilityA1

Methods of treating acne

Assignee: COLLAGENEX PHARM INCPriority: Apr 5, 2001Filed: Jan 14, 2004Published: Jul 29, 2004
Est. expiryApr 5, 2021(expired)· nominal 20-yr term from priority
Inventors:Robert Ashley
A61K 31/135A61K 31/65D06M 16/00A61K 9/0053A61P 17/10
66
PatentIndex Score
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Claims

Abstract

A method of treating acne in a human in need thereof comprising administering systemically to said human a tetracycline compound in an amount that is effective to treat acne but has substantially no antibiotic activity, without administering a bisphosphonate compound.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A method of treating acne in a human in need thereof comprising administering systemically to said human a tetracycline compound in an amount that is effective to treat acne but has substantially no antibiotic activity, without administering a bisphosphonate compound.  
     
     
         2 . A method according to  claim 1 , wherein said acne is acne vulgaris, cystic acne, acne atrophica, bromide acne, chlorine acne, acne conglobata, acne cosmetica, acne detergicans, epidemic acne, acne estivalis, acne fulminans, halogen acne, acne indurata, iodide acne, acne keloid, acne mechanica, acne papulosa, pomade acne, premenstral acne, acne pustulosa, acne rosacea, acne scorbutica, acne scrofulosorum, acne urticata, acne varioliformis, acne venenata, propionic acne, acne excoriee, gram negative acne, steroid acne, or nodulocystic acne.  
     
     
         3 . A method according to  claim 1 , wherein said tetracycline compound is an antibiotic tetracycline compound administered in an amount which is 10-80% of the antibiotic amount.  
     
     
         4 . A method according to  claim 1 , wherein said tetracycline compound is doxycycline administered twice a day in a dose of 20 mg.  
     
     
         5 . A method according to  claim 1 , wherein said tetracycline compound is minocycline administered once a day in a dose of 38 mg.  
     
     
         6 . A method according to  claim 1 , wherein said tetracycline compound is minocycline administered twice a day in a dose of 38 mg.  
     
     
         7 . A method according to  claim 1 , wherein said tetracycline compound is minocycline administered three times a day in a dose of 38 mg.  
     
     
         8 . A method according to  claim 1 , wherein said tetracycline compound is minocycline administered four times a day in a dose of 38 mg.  
     
     
         9 . A method according to  claim 1 , wherein said tetracycline compound is tetracycline administered once a day in a dose of 60 mg/day.  
     
     
         10 . A method according to  claim 1 , wherein said tetracycline compound is tetracycline administered twice a day in a dose of 60 mg/day.  
     
     
         11 . A method according to  claim 1 , wherein said tetracycline compound is tetracycline administered three times a day in a dose of 60 mg/day.  
     
     
         12 . A method according to  claim 1 , wherein said tetracycline compound is tetracycline administered four times a day in a dose of 60 mg/day.  
     
     
         13 . A method according to  claim 1 , wherein said tetracycline compound is an antibiotic tetracycline compound administered in an amount which results in a serum concentration which is 10-80% of the minimum antibiotic serum concentration.  
     
     
         14 . A method according to  claim 1 , wherein said tetracycline compound is doxycycline administered in an amount which results in a serum concentration which is 1.0 μg/ml.  
     
     
         15 . A method according to  claim 1 , wherein said tetracycline compound is minocycline administered in an amount which results in a serum concentration which is 0.8 μg/ml.  
     
     
         16 . A method according to  claim 1 , wherein said tetracycline compound is tetracycline administered in an amount which results in a serum concentration which is 0.5 μg/ml.  
     
     
         17 . A method according to  claim 3  or  13 , wherein said antibiotic tetracycline compound is doxycycline, minocycline, tetracycline, oxytetracycline, chlortetracycline, demeclocycline or pharmaceutically acceptable salts thereof.  
     
     
         18 . A method according to  claim 17 , wherein said antibiotic tetracycline compound is doxycycline.  
     
     
         19 . A method according to  claim 18 , wherein said doxycycline is administered in an amount which provides a serum concentration in the range of about 0.1 to about 0.8 μg/ml.  
     
     
         20 . A method according to  claim 18 , wherein said doxycycline is administered in an amount of 20 milligrams twice daily.  
     
     
         21 . A method according to  claim 19 , wherein said doxycycline is administered by sustained release over a 24 hour period.  
     
     
         22 . A method according to  claim 21 , where said doxcycline is administered in an amount of 40 milligrams.  
     
     
         23 . A method according to  claim 1 , wherein said tetracycline compound is a non-antibiotic tetracycline compound.  
     
     
         24 . A method according to  claim 23 , wherein said non-antibiotic tetracycline compound is: 
 4-de(dimethylamino)tetracycline (CMT-1),    tetracyclinonitrile (CMT-2),    6-demethyl-6-deoxy-4-de(dimethylamino)tetracycline (CMT-3),    4-de(dimethylamino)-7-chlorotetracycline (CMT-4),    tetracycline pyrazole (CMT-5)    4-hydroxy-4-de(dimethylamino)tetracycline (CMT-6),    4-de(dimethylamino)-12α-deoxytetracycline (CMT-7),    6-α-deoxy-5-hydroxy-4-de(dimethylamino)tetracycline (CMT-8),    4-de(dimethylamino)-12α-deoxyanhydrotetracycline (CMT-9), or    4-de(dimethylamino)minocycline (CMT-10).    
     
     
         25 . A method according to  claim 23 , wherein the non-antibiotic tetracycline compound is selected from the group consisting of:  
       
         
           
           
               
               
           
         
       
       wherein: 
 R7 is selected from the group consisting of hydrogen, amino, nitro, mono(lower alkyl)amino, halogen, di(lower alkyl)amino, ethoxythiocarbonylthio, azido, acylamino, diazonium, cyano, and hydroxyl;  
 R6-a is selected from the group consisting of hydrogen and methyl;  
 R6 and R5 are selected from the group consisting of hydrogen and hydroxyl;  
 R8 is selected from the group consisting of hydrogen and halogen;  
 R9 is selected from the group consisting of hydrogen, amino, azido, nitro, acylamino, hydroxy, ethoxythiocarbonylthio, mono(lower alkyl)amino, halogen, diazonium, di(lower alkyl)amino and RCH(NH 2 )CO;  
 R is hydrogen or lower alkyl; and pharmaceutically acceptable salts thereof; with the following provisos:  
 when either R7 and R9 are hydrogen then R8 must be halogen; and  
 when R6-a, R6, R5 and R9 are all hydrogen and R7 is hydrogen, amino, nitro, halogen, dimethylamino or diethylamino, then R8 must be halogen; and  
 when R6-a is methyl, R6 and R9 are both hydrogen, R5 is hydroxyl, and R7 is hydrogen, amino, nitro, halogen or diethylamino, then R8 is halogen; and  
 when R6-a is methyl, R6 is hydroxyl, R5, R7 and R9 are all hydrogen, then R8 must be halogen; and  
 when R6-a, R6 and R5 are all hydrogen, R9 is methylamino and R7 is dimethylamino, then R8 must be halogen; and  
 when R6-a is methyl, R6 is hydrogen, R5 is hydroxyl, R9 is methylamino and R7 is dimethylamino, then R8 must be halogen; and  
 when R6-a is methyl, R6, R5 and R9 are all hydrogen and R7 is cyano, then R8 must be halogen.  
 
     
     
         26 . A method according to  claim 23 , wherein the non-antibiotic tetracycline compound is selected from the group consisting of:  
       
         
           
           
               
               
           
         
       
       wherein: 
 R7 is selected from the group consisting of hydrogen, amino, nitro, mono(lower alkyl)amino, halogen, and di(lower alkyl)amino, ethoxythiocarbonylthio, azido, acylamino, diazonium, cyano, and hydroxyl;  
 R6-a is selected from the group consisting of hydrogen and methyl;  
 R6 and R5 are selected from the group consisting of hydrogen and hydroxyl;  
 R4 is selected from the group consisting of NOH, N—NH-A, and NH-A, where A is a lower alkyl group;  
 R8 is selected from the group consisting of hydrogen and halogen;  
 R9 is selected from the group consisting of hydrogen, amino, azido, nitro, acylamino, hydroxy, ethoxythiocarbonylthio, mono(lower alkyl)amino, halogen, di(lower alkyl)amino and RCH(NH 2 )CO;  
 R is hydrogen or lower alkyl; and  
 pharmaceutically acceptable salts thereof; with the following provisos:  
 when R4 is NOH, N—NH-alkyl or NH-alkyl and R7, R6-a, R6, R5, and R9 are all hydrogen, then R8 must be halogen; and  
 when R4 is NOH, R6-a is methyl, R6 is hydrogen or hydroxyl, R7 is halogen, R5 and R9 are both hydrogen, then R8 must be halogen; and  
 when R4 is N—NH-alkyl, R6-a is methyl, R6 is hydroxyl and R7, R5, R9 are all hydrogen, then R8 must be halogen; and  
 when R4 is NH-alkyl, R6-a, R6, R5 and R9 are all hydrogen, R7 is hydrogen, amino, mono(lower alkyl)amino, halogen, di(lower alkyl)amino or hydroxyl, then R8 must be halogen; and  
 when R4 is NH-alkyl, R6-a is methyl, R6 and R9 are both hydrogen, R5 is hydroxyl; and R7 is mono(lower alkyl)amino or di(lower alkyl)amino, then R8 must be halogen; and  
 when R4 is NH-alkyl, R6-a is methyl, R6 is hydroxy or hydrogen and R7, R5, and R9 are all be hydrogen, then R8 must be halogen.  
 
     
     
         27 . A method according to  claim 23  wherein the non-antibiotic tetracycline compound is selected from the group consisting of:  
       
         
           
           
               
               
           
         
       
       wherein: R7, R8, and R9 taken together in each case, have the following meanings:  
       
         
           
                 
                 
                 
                 
               
                     
                     
                 
                     
                     
                 
                     
                   R7 
                   R8 
                   R9 
                 
                     
                     
                 
                     
                   azido 
                   hydrogen 
                   hydrogen 
                 
                     
                   dimethylamino 
                   hydrogen 
                   azido 
                 
                     
                   hydrogen 
                   hydrogen 
                   amino 
                 
                     
                   hydrogen 
                   hydrogen 
                   azido 
                 
                     
                   hydrogen 
                   hydrogen 
                   nitro 
                 
                     
                   dimethylamino 
                   hydrogen 
                   amino 
                 
                     
                   acylamino 
                   hydrogen 
                   hydrogen 
                 
                     
                   hydrogen 
                   hydrogen 
                   acylamino 
                 
                     
                   amino 
                   hydrogen 
                   nitro 
                 
                     
                   hydrogen 
                   hydrogen 
                   (N,N-dimethyl)glycylamino 
                 
                     
                   amino 
                   hydrogen 
                   amino 
                 
                     
                   hydrogen 
                   hydrogen 
                   ethoxythiocarbonylthio 
                 
                     
                   dimethylamino 
                   hydrogen 
                   acylamino 
                 
                     
                   dimethylamino 
                   hydrogen 
                   diazonium 
                 
                     
                   dimethylamino 
                   chloro 
                   amino 
                 
                     
                   hydrogen 
                   chloro 
                   amino 
                 
                     
                   amino 
                   chloro 
                   amino 
                 
                     
                   acylamino 
                   chloro 
                   acylamino 
                 
                     
                   amino 
                   chloro 
                   hydrogen 
                 
                     
                   acylamino 
                   chloro 
                   hydrogen 
                 
                     
                   monoalkylamino 
                   chloro 
                   amino 
                 
                     
                   nitro 
                   chloro 
                   amino 
                 
                     
                   dimethylamino 
                   chloro 
                   acylamino 
                 
                     
                   dimethylamino 
                   chloro 
                   dimethylamino 
                 
                     
                   hydrogen 
                   hydrogen 
                   dimethylamino 
                 
                     
                   dimethylamino 
                   hydrogen 
                   hydrogen 
                 
                     
                   and 
                 
                 
               
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                   wherein: R7, R8, and R9 taken together in each case, have the following 
                 
                   meanings: 
                 
                 
                 
                 
                 
               
                     
                   azido 
                   hydrogen 
                   hydrogen 
                 
                     
                   dimethylamino 
                   hydrogen 
                   azido 
                 
                     
                   hydrogen 
                   hydrogen 
                   amino 
                 
                     
                   hydrogen 
                   hydrogen 
                   azido 
                 
                     
                   hydrogen 
                   hydrogen 
                   nitro 
                 
                     
                   dimethylamino 
                   hydrogen 
                   amino 
                 
                     
                   acylamino 
                   hydrogen 
                   hydrogen 
                 
                     
                   hydrogen 
                   hydrogen 
                   acylamino 
                 
                     
                   amino 
                   hydrogen 
                   nitro 
                 
                     
                   hydrogen 
                   hydrogen 
                   (N,N-dimethyl)glycylamino 
                 
                     
                   amino 
                   hydrogen 
                   amino 
                 
                     
                   hydrogen 
                   hydrogen 
                   ethoxythiocarbonylthio 
                 
                     
                   dimethylamino 
                   hydrogen 
                   acylamino 
                 
                     
                   hydrogen 
                   hydrogen 
                   diazonium 
                 
                     
                   hydrogen 
                   hydrogen 
                   dimethylamino 
                 
                     
                   diazonium 
                   hydrogen 
                   hydrogen 
                 
                     
                   ethoxythiocarbonylthio 
                   hydrogen 
                   hydrogen 
                 
                     
                   dimethylamino 
                   chloro 
                   amino 
                 
                     
                   amino 
                   chloro 
                   amino 
                 
                     
                   acylamino 
                   chloro 
                   acylamino 
                 
                     
                   hydrogen 
                   chloro 
                   amino 
                 
                     
                   amino 
                   chloro 
                   hydrogen 
                 
                     
                   acylamino 
                   chloro 
                   hydrogen 
                 
                     
                   monoalkylamino 
                   chloro 
                   amino 
                 
                     
                   nitro 
                   chloro 
                   amino 
                 
                     
                   and 
                 
                 
               
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                   wherein: R8 is hydrogen or halogen and R9 is selected from the group 
                 
                   consisting of nitro, (N,N-dimethyl)glycylamino, and 
                 
                   ethoxythiocarbonylthio; and 
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                   wherein: R7, R8, and R9 taken together in each case, have the following 
                 
                   meanings: 
                 
                 
                 
                 
                 
               
                     
                   amino 
                   hydrogen 
                   hydrogen 
                 
                     
                   nitro 
                   hydrogen 
                   hydrogen 
                 
                     
                   azido 
                   hydrogen 
                   hydrogen 
                 
                     
                   dimethylamino 
                   hydrogen 
                   azido 
                 
                     
                   hydrogen 
                   hydrogen 
                   amino 
                 
                     
                   hydrogen 
                   hydrogen 
                   azido 
                 
                     
                   hydrogen 
                   hydrogen 
                   nitro 
                 
                     
                   bromo 
                   hydrogen 
                   hydrogen 
                 
                     
                   dimethylamino 
                   hydrogen 
                   amino 
                 
                     
                   acylamino 
                   hydrogen 
                   hydrogen 
                 
                     
                   hydrogen 
                   hydrogen 
                   acylamino 
                 
                     
                   amino 
                   hydrogen 
                   nitro 
                 
                     
                   hydrogen 
                   hydrogen 
                   (N,N-dimethyl)glycylamino 
                 
                     
                   amino 
                   hydrogen 
                   amino 
                 
                     
                   diethylamino 
                   hydrogen 
                   hydrogen 
                 
                     
                   hydrogen 
                   hydrogen 
                   ethoxythiocarbonylthio 
                 
                     
                   dimethylamino 
                   hydrogen 
                   methylamino 
                 
                     
                   dimethylamino 
                   hydrogen 
                   acylamino 
                 
                     
                   dimethylamino 
                   chloro 
                   amino 
                 
                     
                   amino 
                   chloro 
                   amino 
                 
                     
                   acylamino 
                   chloro 
                   acylamino 
                 
                     
                   hydrogen 
                   chloro 
                   amino 
                 
                     
                   amino 
                   chloro 
                   hydrogen 
                 
                     
                   acylamino 
                   chloro 
                   hydrogen 
                 
                     
                   monoalkylamino 
                   chloro 
                   amino 
                 
                     
                   nitro 
                   chloro 
                   amino 
                 
                     
                     
                 
                     
                     
                 
             
                
                
                
                
               
               
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
             
                
                
                
                
                
                
                
                
                
                
               
            
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
             
                
                
                
                
                
                
                
                
                
                
               
            
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
       and pharmaceutically acceptable salts thereof.  
     
     
         28 . A method according to  claim 23 , wherein the non-antibiotic tetracycline compound is selected from the group consisting of:  
       
         
           
           
               
               
           
         
       
       wherein: 
 R7 is selected from the group consisting of hydrogen, amino, nitro, mono(lower alkyl)amino, halogen, di(lower alkyl)amino, ethoxythiocarbonylthio, azido, acylamino, diazonium, cyano, and hydroxyl;  
 R6-a is selected from the group consisting of hydrogen and methyl;  
 R6 and R5 are selected from the group consisting of hydrogen and hydroxyl;  
 R8 is selected from the group consisting of hydrogen and halogen;  
 R9 is selected from the group consisting of hydrogen, amino, azido, nitro, acylamino, hydroxy, ethoxythiocarbonylthio, mono(lower alkyl)amino, halogen, diazonium, di(lower allyl)amino and RCH(NH 2 )CO;  
 R is hydrogen or lower alkyl;  
 R a  and R b  are selected from the group consisting of hydrogen, methyl, ethyl, n-propyl and 1-methylethyl with the proviso that R a  and R b  cannot both be hydrogen;  
 R c  and R d  are, independently, (CH 2 ) n CHR e  wherein n is 0 or 1 and R e  is selected from the group consisting of hydrogen, alkyl, hydroxy, lower(C 1 -C 3 )alkoxy, amino, or nitro; and,  
 W is selected from the group consisting of (CHR e ) m  wherein m is 0-3 and said R e  is as above, NH, N(C 1 -C 3 ) straight chained or branched alkyl, 0, S and N(C 1 -C 4 ) straight chain or branched alkoxy; and,  
 pharmaceutically acceptable salts thereof.  
 
     
     
         29 . A method according to  claim 16 , wherein the non-antibiotic tetracycline compound selected from the group consisting of structures S-Z has the following provisos: 
 when either R7 and R 9  are hydrogen then R8 must be halogen; and    when R6-a, R6, R5 and R9 are all hydrogen and R7 is hydrogen, amino, nitro, halogen, dimethylamino or diethylamino, then R8 must be halogen; and    when R6-a is methyl, R6 and R9 are both hydrogen, R5 is hydroxyl, and R7 is hydrogen, amino, nitro, halogen or diethylamino, then R8 is halogen; and    when R6-a is methyl, R6 is hydroxyl, R5, R7 and R9 are all hydrogen, then R8 must be halogen; and    when R6-a, R6 and R5 are all hydrogen, R9 is methylamino and R7 is dimethylamino, then R8 must be halogen; and    when R6-a is methyl, R6 is hydrogen, R5 is hydroxyl, R9 is methylamino and R7 is dimethylamino, then R8 must be halogen; and    when R6-a is methyl, R6, R5 and R9 are all hydrogen and R7 is cyano, then R8 must be halogen.    
     
     
         30 . A method according to  claim 1 , wherein said tetracycline compound has a photoirritancy factor of less than the photoirritancy factor of doxycycline.  
     
     
         31 . A method according to  claim 1 , wherein said tetracycline compound has a photoirritancy factor from about one to about two.  
     
     
         32 . A method according to  claim 31 , wherein said tetracycline compound has a general formula:  
       
         
           
           
               
               
           
         
       
       wherein R7, R8, and R9 taken together are, respectively, hydrogen, hydrogen and dimethylamino.  
     
     
         33 . A method according to  claim 1 , wherein said tetracycline compound has a photoirritancy factor from about 1.0 to about 1.2.  
     
     
         34 . A method according to  claim 33 , wherein said tetracycline compound is selected from the group consisting of:  
       
         
           
                 
                 
                 
                 
               
                     
                     
                 
                     
                     
                 
                     
                   R7 
                   R8 
                   R9 
                 
                     
                     
                 
                     
                 
                 
               
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                   wherein R7, R8, and R9 taken together in each case, have the following 
                 
                   meanings: 
                 
                 
                 
                 
                 
               
                     
                   hydrogen 
                   hydrogen 
                   amino 
                 
                     
                   hydrogen 
                   hydrogen 
                   palmitamide 
                 
                     
                   and 
                 
                 
               
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                   wherein R7, R8, and R9 taken together in each case, have the following 
                 
                   meanings: 
                 
                 
                 
                 
                 
               
                     
                   hydrogen 
                   hydrogen 
                   acetamido 
                 
                     
                   hydrogen 
                   hydrogen 
                   dimethylaminoacetamido 
                 
                     
                   hydrogen 
                   hydrogen 
                   nitro 
                 
                     
                   hydrogen 
                   hydrogen 
                   amino 
                 
                     
                   and 
                 
                 
               
                     
                 
                   
                     
                       
                       
                           
                           
                       
                     
                   
                 
                   wherein R8, and R9 taken together are, respectively, hydrogen and nitro. 
                 
                     
                 
                     
                 
             
                
                
                
                
               
               
                
               
            
             
                
                
                
                
               
            
             
                
                
                
               
            
             
                
                
                
                
                
                
                
                
                
                
               
            
             
                
                
                
                
                
               
            
             
                
                
                
                
                
               
            
           
         
       
     
     
         35 . A method according to  claim 1 , wherein said systemic administration is oral administration, intravenous injection, intramuscular injection, subcutaneous administration, transdermal administration or intranasal administration.  
     
     
         36 . A method of treating acne in a human in need thereof comprising administering to said human an effective amount of a non-antibiotic tetracycline compound without administering a bisphosphonate compound.  
     
     
         37 . A method according to  claim 36 , wherein said administration is topical administration.  
     
     
         38 . A method according to  claim 36 , wherein said administration is systemic administration.  
     
     
         39 . A method for reducing the number of comedones in a human in need thereof comprising administering systemically to said human a tetracycline compound in an amount that is effective to reduce the number of comedones but has substantially no antibiotic activity.  
     
     
         40 . A method according to  claim 39 , wherein said tetracycline compound is doxycycline.  
     
     
         41 . A method according to  claim 40 , wherein said doxycycline is administered in a daily amount of from about 30 to about 60 milligrams but maintains a concentration in human plasma below the threshold for a significant antibiotic effect.  
     
     
         42 . A method according to  claim 40 , wherein said doxycycline is administered in an amount of approximately 20 milligrams twice daily.  
     
     
         43 . A method according to  claim 39 , wherein said tetracycline compound is administered without administering a bisphosphonate.  
     
     
         44 . A method for inhibiting oxidation of melanin in a human in need thereof comprising administering systemically to said human a tetracycline compound in an amount that is effective to inhibit oxidation of melanin but has substantially no antibiotic activity.  
     
     
         45 . A method for inhibiting lipid-associated abnormal follicular differentiation in a human in need thereof comprising administering systemically to said, human a tetracycline compound in an amount that is effective to inhibit lipid-associated abnormal follicular differentiation but has substantially no antibiotic activity.

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