US2004167102A1PendingUtilityA1

Isoprenoid analog compounds and methods of making and use thereof

57
Assignee: UNIV IOWA RES FOUNDPriority: Apr 3, 2001Filed: Feb 17, 2004Published: Aug 26, 2004
Est. expiryApr 3, 2021(expired)· nominal 20-yr term from priority
A61K 49/0021C07F 9/113C07F 9/3839C07F 9/4015C07F 9/098
57
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Claims

Abstract

The invention provides compounds of formula I: wherein X, R 1 , R 2 , and n have any of the values defined in the specification, and their pharmaceutically acceptable salts. The compounds are useful, for example, for blocking prenylation transferase enzymes, for probing or diagnosing protein prenylation processes, and for treating cancer in a mammal. The invention also provides pharmaceutical compositions, processes for preparing compounds of formula I, and intermediates useful for the synthesis of compounds of formula I.

Claims

exact text as granted — not AI-modified
What is claimed is:  
     
         1 . A compound of formula I:  
       
         
           
           
               
               
           
         
       
       wherein: 
 X is independently O or S;  
 R 1  is a detectable group;  
 R 2  is independently  
 OH,  
 (C 1 -C 10 )alkanoyloxy,  
 —O—P(═O)(—OR a ) 2 ,  
 —O—P(═O)(—OR a )—O—P(═O)(—OR a ) 2 ,  
 —CH 2 —O—P(═O)(—OR a ) 2 ,  
 —CH 2 —O—P(═O)(—OR a )—O—P(═O)(—OR a ) 2 ,  
 —CH 2 —P(═O)(—OR a ) 2 ,  
 —CH{—P(═O)(—OR a ) 2 } 2 ,  
 —CH 2 —P(═O)(—OR a )—O—P(═O)(—OR a ) 2 ,  
 —CH═CH{—P(═O)(—OR a ) 2 }, or  
 —CH═C{—P(═O)(—OR a ) 2 } 2 ;  
 each R a  is independently hydrogen, (C 1 -C 10 )alkyl, (C 1 -C 10 )alkanoyl, (C 1 -C 10 )alkanoyloxy, (C 1 -C 10 )alkoxycarbonyl, or —CH 2 —O—(C 1 -C 10 )alkanoyl;  
 n is independently 1, 2, or 3;  
 or a pharmaceutically acceptable salt thereof.  
 
     
     
         2 . The compound of  claim 1  wherein the detectable group is aryl or Het, optionally substituted with one or more substituents independently selected from —COOR b , —S(O) n NR b R b , halo, cyano, nitro, aryl, heterocycle, (C 1 -C 10 )alkoxy, (C 2 -C 6 )alkenyl, —C(═O)NR b R b , —OC(═O)NR b R b , —NR b R b , or —S(O) n R b , where each R b  is independently hydrogen, (C 1 -C 10 )alkyl, or (C 1 -C 10 )alkanoyl.  
     
     
         3 . The compound of  claim 2  wherein aryl or Het is phenyl, indenyl, naphthyl, anthracenyl, or anthranil, which aryl or Het is optionally substituted with one or more substituents independently selected from —COOR b , —S(O) n NR b R b , halo, cyano, nitro, aryl, heterocycle, (C 2 -C 6 )alkenyl, —C(═O)NR b R b , —OC(═O)NR b R b , —NR b R b , or —S(O) n R b , where each R b  is independently hydrogen, (C 1 -C 10 )alkyl, or (C 1 -C 10 )alkanoyl.  
     
     
         4 . The compound of  claim 1  wherein R 1  is substituted phenyl.  
     
     
         5 . The compound of  claim 1  wherein R 1  is phenyl substituted with —COOR b .  
     
     
         6 . The compound of  claim 1  wherein R 1  is 2-methoxycarboxy phenyl.  
     
     
         7 . The compound of  claim 1  wherein R 1  is substituted naphthyl.  
     
     
         8 . The compound of  claim 1  wherein R 1  is naphthyl substituted with a —S(O) n NR b R b .  
     
     
         9 . The compound of  claim 1  wherein R 1  is naphthyl substituted at the 5-position with a —S(O) n NR b R b  substituent.  
     
     
         10 . The compound of  claim 1  wherein R 1  is 5-N,N′—dimethylaminosulfonyl naphthy-1-yl.  
     
     
         11 . The compound of  claim 1  wherein R 2  is OH.  
     
     
         12 . The compound of  claim 1  wherein R 2  is (C 1 -C 10 )alkanoyloxy.  
     
     
         13 . The compound of  claim 1  wherein R 2  is —O—P(═O)(—OR a ) 2 .  
     
     
         14 . The compound of  claim 1  wherein R 2  is —O—P(═O)(—OR a )—O—P(═O)(—OR a ) 2 .  
     
     
         15 . The compound of  claim 1  wherein R 2  is —CH 2 —O—P(═O)(—OR a ) 2 .  
     
     
         16 . The compound of  claim 1  wherein R 2  is —CH 2 —O—P(═O)(—OR a )—O—P(═O)(—OR a ) 2 .  
     
     
         17 . The compound of  claim 1  wherein R 2  is —CH 2 —P(═O)(—OR a ) 2 .  
     
     
         18 . The compound of  claim 1  wherein R 2  is —CH{—P(═O)(—OR a ) 2 } 2 .  
     
     
         19 . The compound of  claim 1  wherein R 2  is —CH 2 —P(═O)(—OR a )—O—P(═O)(—OR a ) 2 .  
     
     
         20 . The compound of  claim 1  wherein R 2  is —CH═CH{—P(═O)(—OR a ) 2 }.  
     
     
         21 . The compound of  claim 1  wherein R 2  is —CH═C {—P(═O)(—OR a ) 2 } 2 .  
     
     
         22 . The compound of  claim 1  wherein R a  is hydrogen.  
     
     
         23 . The compound of  claim 1  wherein R a  is —C(═O)—CH 3 .  
     
     
         24 . The compound of  claim 1  wherein R a  is —CH 3 .  
     
     
         25 . The compound of  claim 1  wherein R a  is —CH 2 —O—(C 1 -C 6 )alkanoyl.  
     
     
         26 . The compound of  claim 2  wherein R b  is hydrogen.  
     
     
         27 . The compound of  claim 2  wherein R b  is —CH 3 .  
     
     
         28 . The compound of  claim 1  wherein n is 1.  
     
     
         29 . The compound of  claim 1  wherein n is 2.  
     
     
         30 . The compound of  claim 1  wherein n is 3.  
     
     
         31 . The compound of  claim 1  wherein X is —O—.  
     
     
         32 . The compound of  claim 1  wherein X is —S—.  
     
     
         33 . A pharmaceutical composition comprising a compound as described in  claim 1  and a pharmaceutically acceptable diluent or carrier.  
     
     
         34 . A method of treating cancer, comprising administering to a mammal afflicted with cancer, an amount of a compound as described in  claim 1  effective to treat said cancer.  
     
     
         35 . A method of inhibiting a prenylation transferase enzyme or synthase enzyme comprising contacting the enzyme with an effective amount of a compound as described in  claim 1 .  
     
     
         36 . A method of accessing the metabolic status of an enzyme comprising: 
 contacting the enzyme with an effective amount of a mixture of a farnesol analog compound and a geraniol or geranylgeraniol analog compound as described in  claim 1;  and    measuring the relative ratio of farnesylation to geranylgeranylation of the famesol and the geraniol or geranylgeraniol analog compounds accomplished by the enzyme.    
     
     
         37 . A compound as described in  claim 1  for use in medical therapy or diagnosis.  
     
     
         38 . The compound of  claim 37  wherein the therapy or diagnosis is treating cancer.  
     
     
         39 . The use of a compound as described in  claim 1  for the manufacture of a medicament useful for the treatment of cancer.  
     
     
         40 . The use of a compound as described in  claim 1  for the manufacture of a medicament useful for inhibiting prenylation transferase enzymes in a mammal.  
     
     
         41 . A protein conjugate comprising a protein linked to a fluorescent fragment of a compound of  claim 1.

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