US2004194159A1PendingUtilityA1
Cloning pigs using donor nuclei from differentiated cells
Est. expiryJan 10, 2017(expired)· nominal 20-yr term from priority
A61P 9/04A61P 31/18A61P 9/00A61P 3/10A61P 35/00A61P 25/16A61P 25/28A61P 25/00C12N 15/8771A61P 1/18A61P 21/00C12N 2517/04A61P 19/00C12N 2510/00A01K 67/0275A61K 35/12A01K 2227/101A01K 67/0273A61K 38/00C12N 15/8778A01K 2217/05C12N 2517/02A61P 13/02C12N 5/0603A61P 1/16A01K 67/027A61P 17/00A61P 17/02
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Abstract
An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
Claims
exact text as granted — not AI-modified1 . A method of cloning a pig, comprising:
(i) inserting a desired differentiated pig cell or cell nucleus into an enucleated pig oocyte, under conditions suitable for the formation of a nuclear transfer (NT) unit; (ii) activating the resultant nuclear transfer unit; and (iii) transferring said cultured NT unit to a host mammal such that the NT unit develops into a fetus.
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