US2004197872A1PendingUtilityA1

Infectious clones of RNA viruses and vaccines and diagnostic assays derived thereof

56
Priority: Oct 30, 1996Filed: Dec 30, 2003Published: Oct 7, 2004
Est. expiryOct 30, 2016(expired)· nominal 20-yr term from priority
Inventors:Johanna MeulenbergJohannes Maria Antonius PolJudy Norma Aletta Bos-De Ruijter
C12N 7/00C12N 2770/10034C07K 14/005C12N 2770/10022A61K 39/00C12N 15/11A61P 37/04C12Q 1/68A61K 39/12
56
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Claims

Abstract

An infectious clone based on the genome of a wild-type RNA virus is produced by the process of providing a host cell not susceptible to infection by the wild-type RNA virus, providing a recombinant nucleic acid based on the genome of the wild-type RNA virus, transfecting the host cell with the recombinant nucleic acid and selecting for infectious clones. The recombinant nucleic acid comprises at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either. The infectious clones can be used in single or dual purpose vaccines and in viral vector vaccines.

Claims

exact text as granted — not AI-modified
1 - 20 . (canceled)  
     
     
         21 . An isolated polynucleotide molecule comprising a DNA sequence encoding an infectious RNA molecule encoding a United States strain PRRS virus.  
     
     
         22 . An isolated polynucleotide molecule comprising a DNA sequence encoding an infectious RNA molecule encoding a PRRS virus selected from the group consisting of PRRS virus strains ATCC VR 2332, ATCC VR 2385, ATCC VR 2386, ATCC VR 2429, ATCC VR 2474, and ATCC VR 2402.  
     
     
         23 . A transfected cell comprising a DNA sequence encoding an infectious RNA molecule encoding a PRRS virus selected from the group consisting of PRRS virus strains ATCC VR 2332, ATCC VR 2385, ATCC VR 2386, ATCC VR 2429, ATCC VR 2474, and ATCC VR 2402, which transfected cell is capable of expressing the encoded PRRS virus.  
     
     
         24 . An isolated polynucleotide molecule in the form of a plasmid, wherein said isolated polynucleotide molecule comprises a DNA sequence encoding an infectious RNA molecule encoding a PRRS virus selected from the group consisting of PRRS virus strains ATCC VR 2332, ATCC VR 2385, ATCC VR 2386, ATCC VR 2429, ATCC VR 2474, and ATCC VR 2402.  
     
     
         25 . An isolated infectious RNA molecule encoded by an isolated polynucleotide molecule, which infectious RNA molecule encodes a PRRS virus selected from the group consisting of PRRS virus strains ATCC VR 2332, ATCC VR 2385, ATCC VR 2386, ATCC VR 2429, ATCC VR 2474, and ATCC VR 2402.  
     
     
         26 . A recombinant PRRS virus encoded by an isolated polynucleotide molecule comprising a DNA sequence encoding an infectious RNA molecule encoding a PRRS virus selected from the group consisting of PRRS virus strains ATCC VR 2332, ATCC VR 2385, ATCC VR 2386, ATCC VR 2429, ATCC VR 2474, and ATCC VR 2402.  
     
     
         27 . An isolated polynucleotide molecule comprising a DNA sequence encoding an infectious RNA molecule encoding a PRRS virus wherein said PRRS virus comprises ORF7 protein of ATCC VR2332.

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