Method for isolating phenolic substances or javabiones from wood comprising knotwood

Abstract

The present invention relates to a method for isolating of phenolic substances or juvabiones from wood comprising knotwood, said method comprising the steps of a) extracting i) the over-sized chip fraction obtained by screening chipped wood, or ii) a knot-rich sub-fraction obtained from said over-sized chip fraction, or iii) knotwood obtained as residue in finishing of mechanical wood products with a polar solvent, and b) recovering the extract.

1 - 21 . (canceled)  
     
     
         22 . A method for isolating phenolic substances or juvabiones from wood comprising knotwood, said method comprising the steps of 
 a) extracting 
 i) the over-sized chip fraction obtained by screening chipped wood, or  
 ii) a knot-rich sub-fraction obtained from said over-sized chip fraction, or  
 iii) knotwood obtained as residue in finishing of mechanical wood products  
   with a polar solvent, and    b) recovering the extract.    
     
     
         23 . The method of  claim 22 , wherein the over-sized chip fraction is separated in a knot-rich fraction and a knot-poor fraction, and the knot-rich fraction is extracted.  
     
     
         24 . The method of  claim 23 , wherein the over-sized chip fraction is refined before the separation.  
     
     
         25 . The method of  claim 22 , wherein the polar solvent is either a single polar agent, or a mixture of two or more polar agents, said polar agent or agents having a dielectric constant that is greater than 3, determined at 25 Celsius degrees.  
     
     
         26 . The method of  claim 22 , wherein the extract is further purified for releasing the desired phenolic substance or juvabione.  
     
     
         27 . The method of  claim 22 , wherein a) the phenolic substance is 
 i) a lignan, which is hydroxymatairesinol, allohydroxy-matairesinol, matairesinol, conidendrin, pinoresinol, oxomatairesinol, lariciresinol, liovil, isolariciresinol, secoisolariciresinol, picearesinol, conidendric acid or nortrachelogenin, or    ii) a flavonoid, which is pinocembrin, dihydrokaempferol, pinobanksin, naringenin, catechin, 2,4,6-trihydroxychalcone, aromadendrin or taxifolin, or    iii) a stilbene, which is pinosylvin, pinosylvin monomethyl ether, dihydropinosylvin, methylpinosylvin, methyldihydropinosylvin or reservatrol, or    b) the juvabione is epijuvabione, dehydrojuvabione, dihydroepijuvabione or epijuvabione acid.    
     
     
         28 . The method of  claim 22 , wherein the wood comprises coniferous wood.  
     
     
         29 . The method of  claim 28 , wherein the wood is spruce wood of the genus Picea.  
     
     
         30 . The method of  claim 29 , wherein the isolated substance is a lignan which is hydroxymatairesinol, secoisolariciresinol or liovil.  
     
     
         31 . The method of  claim 28 , wherein the wood is fir wood of the genus Abies.  
     
     
         32 . The method of  claim 31 , wherein the isolated substance is a lignan which is secoisolariciresinol, lariciresinol or isolariciresinol.  
     
     
         33 . The method of  claim 32 , wherein the isolated substance is epijuvabione or dehydrojuvabione.  
     
     
         34 . The method of  claim 28 , wherein the wood is a Larix genus.  
     
     
         35 . The method of  claim 34 , wherein the isolated substance is a lignan which is secoisolariciresinol, lariciresinol or isolariciresinol; or a flavonoid which is catechin, taxifolin or dihydrokaempferol.  
     
     
         36 . The method of  claim 28 , wherein the wood is pine wood of the genus Pinus.  
     
     
         37 . The method of  claim 36 , wherein the isolated substance is a flavoinoid, a lignan, a stilbene or a juvabione.  
     
     
         38 . The method of  claim 37 , wherein the flavoinoid is pinocembrin, dihydrokaempferol, pinobanksin, naringenin, catechin, 2,4,6-trihydroxychalcone, aromadendrin or taxifolin, the lignan is lariciresinol, isolariciresinol, secoisolariciresinol, matairesinol, liovil or nortrachelogenin (NTG), the stilbene is pinosylvin, dihydropinosylvin, pinosylvin monomethyl ether, methylpinosylvin, methyldihydropinosylvin, or reservatrol, or the juvabione is epijuvabione acid.  
     
     
         39 . The method of  claim 28 , wherein the wood is a Pseudotsuga genus.  
     
     
         40 . The method of  claim 39 , wherein the isolated substance is a lignan, which is lariciresinol, isolariciresinol or secoisolariciresinol, or ii) a juvabione such as dihydroepijuvabione.  
     
     
         41 . The method of  claim 22 , wherein the wood is hardwood and the isolated substance is a flavonoid or a stilbene.  
     
     
         42 . The method of  claim 41 , wherein the hardwood is aspen and the isolated and the isolated flavonoid is naringenin or dihydrokaemferol.