Actinobacillus pleuropneumoniae subunit vaccine

Abstract

The present invention relates to live attenuated bacteria of the genus Actinobacillus pleuropneumoniae that have a mutation in an apxIV gene such that no functional ApxIV toxin can be produced. The invention also relates to methods for the production of such bacteria. Also vaccines comprising such bacteria and methods for the production of such vaccines are part of the invention. The invention further relates to subunit vaccines comprising an ApxIV toxin, to methods for the production of such vaccines and to methods for the protection of animals against infection with bacteria of the genus Actinobacillus pleuropneumoniae . In addition, the invention relates to the promotor of the apxIV gene. Finally, the invention relates to diagnostic tests for the selective diagnosis of Actinobacillus pleuropneumoniae infections and to diagnostic tests discriminating between Actinobacillus pleuropneumoniae field strains and vaccine strains.

1 - 6 . (canceled)  
     
     
         7 . A nucleotide sequence harbouring the promoter controlling the expression of the apxIV gene.  
     
     
         8 . The nucleotide sequence according to  claim 7 , characterized in that it comprises the DNA fragment from position 451 to 1132 of SEQ ID NO: 5 or a subfragment thereof still having promoter activity.  
     
     
         9 . The nucleotide sequence according to  claim 7 , characterized in that it comprises the DNA fragment FROM POSITION 617 TO 641 OF SEQ ID NO:5.  
     
     
         10 - 19 . (canceled)