US2004209337A1PendingUtilityA1

Synthesis of 1,2,3,4-tetrahydroxybenzenes from biomass-derived carbon

55
Assignee: FROST JOHN WPriority: Mar 23, 1999Filed: May 4, 2004Published: Oct 21, 2004
Est. expiryMar 23, 2019(expired)· nominal 20-yr term from priority
Inventors:John W. FrostChad Allen Hansen
C12P 7/26C12P 7/02C12P 7/22
55
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

A bioengineered synthesis scheme for the production of 1,2,3,4-tetrahydroxybenzene from a carbon source is provided. Methods of producing 1,2,3,4-tetrahydroxybenzene acid from a carbon source based on the synthesis scheme are also provided. Methods are also provided for converting 1,2,3,4-tetrahydroxybenzene to 1,2,3-trihydroxybenzene by catalytic hydrogenation.

Claims

exact text as granted — not AI-modified
We claim:  
     
         1 . A method for the production of 1,2,3,4-tetrahydroxybenzene, comprising: 
 a) incubating, in the presence of a carbon source, a microbe comprising a genome, a DNA encoding a naturally occurring myo-inositol-1-phosphate synthase and a DNA encoding a naturally occurring inositol dehydrogenase, to produce myo-2-inosose; and    b) converting the myo-2-inosose to 1,2,3,4-tetrahydroxybenzene by acid catalyzed dehydration.    
     
     
         2 . The method of  claim 1 , wherein the DNA encoding the naturally occurring myo-inositol-1-phosphate synthase comprises an INO1 gene.  
     
     
         3 . The method of  claim 2 , wherein the INO1 gene comprises a  Saccharomyces cerevisiae  INO1.  
     
     
         4 . The method of  claim 1 , wherein the DNA encoding the naturally occurring inositol dehydrogenase comprises an iolG gene.  
     
     
         5 . The method of  claim 1 , wherein the DNA encoding the naturally occurring inositol dehydrogenase comprises a  Bacillus subtilis  iolG.  
     
     
         6 . The method of  claim 1 , wherein the DNA encoding the naturally occurring myo-inositol-1-phosphate synthase and the DNA encoding the naturally occurring inositol dehydrogenase are comprised by plasmid pAD2.28A.  
     
     
         7 . The method of  claim 1 , wherein the microbe further comprises a DNA encoding a naturally occurring myo-2-inosose dehydratase.  
     
     
         8 . The method of  claim 7 , wherein the DNA encoding a naturally occurring myo-2-inosose dehydratase is a recombinant DNA encoding a naturally occurring myo-2-inosose dehydratase.  
     
     
         9 . The method of  claim 1 , wherein the microbe further comprises a DNA encoding a naturally occurring inositol monophosphatase.  
     
     
         10 . The method of  claim 9 , wherein the DNA encoding a naturally occurring inositol monophosphatase is a recombinant DNA encoding a naturally occurring inositol monophosphatase.  
     
     
         11 . The method of  claim 1 , wherein the DNA encoding a naturally occurring myo-inositol-1-phosphate synthase is a recombinant DNA encoding a naturally occurring myo-inositol-1-phosphate synthase.  
     
     
         12 . The method of  claim 1 , wherein the DNA encoding a naturally occurring inositol dehydrogenase is a recombinant DNA encoding a naturally occurring inositol dehydrogenase.  
     
     
         13 . The method of  claim 1 , wherein the microbe is an  Escherichia coli.    
     
     
         14 . The method of  claim 1 , wherein the  Escherichia coli  is  Escherichia coli  JWF1/pAD2.28A.  
     
     
         15 . The method of  claim 1 , wherein the genome comprises the DNA encoding a naturally occurring myo-inositol-1-phosphate synthase.  
     
     
         16 . The method of  claim 1 , wherein the genome comprises the DNA encoding a naturally occurring inositol dehydrogenase.  
     
     
         17 . The method of  claim 7 , wherein the genome comprises the DNA encoding a naturally occurring myo-2-inosose dehydratase.  
     
     
         18 . The method of  claim 9 , wherein the genome comprises the DNA encoding a naturally occurring inositol monophosphatase.  
     
     
         19 . The method of  claim 1 , wherein the carbon source comprises glucose.  
     
     
         20 . A microbe comprising a genome, a recombinant DNA encoding a naturally occurring myo-inositol-1-phosphate synthase and a recombinant DNA encoding a naturally occurring inositol dehydrogenase.  
     
     
         21 . The microbe of  claim 20 , wherein the DNA encoding the naturally occurring myo-inositol-1-phosphate synthase comprises an INO1 gene.  
     
     
         22 . The microbe of  claim 21 , wherein the INO1 gene comprises a  Saccharomyces cerevisiae  INO1.  
     
     
         23 . The microbe of  claim 20 , wherein the DNA encoding the naturally occurring inositol dehydrogenase comprises an iolG gene.  
     
     
         24 . The microbe of  claim 20 , wherein the DNA encoding the naturally occurring inositol dehydrogenase comprises a  Bacillus subtilis  iolG.  
     
     
         25 . The microbe of  claim 20 , wherein the DNA encoding the naturally occurring myo-inositol-1-phosphate synthase and the DNA encoding the naturally occurring inositol dehydrogenase are comprised by plasmid pAD2.28A.  
     
     
         26 . The microbe of  claim 20 , wherein the microbe further comprises a DNA encoding a naturally occurring myo-2-inosose dehydratase.  
     
     
         27 . The microbe of  claim 20 , wherein the microbe further comprises a DNA encoding a naturally occurring inositol monophosphatase.  
     
     
         28 . The microbe of  claim 20 , wherein the DNA encoding a naturally occurring myo-inositol-1-phosphate synthase is a recombinant DNA encoding a naturally occurring myo-inositol-1-phosphate synthase.  
     
     
         29 . The microbe of  claim 20 , wherein the DNA encoding a naturally occurring inositol dehydrogenase is a recombinant DNA encoding a naturally occurring inositol dehydrogenase.  
     
     
         30 . The microbe of  claim 20 , wherein the microbe is an  Escherichia coli.    
     
     
         31 . The microbe of  claim 20 , wherein the  Escherichia coli  is  Escherichia coli  JWF1/pAD2.28A.  
     
     
         32 . The microbe of  claim 20 , wherein the genome comprises the DNA encoding a naturally occurring myo-inositol-1-phosphate synthase.  
     
     
         33 . The microbe of  claim 20 , wherein the genome comprises the DNA encoding a naturally occurring inositol dehydrogenase.  
     
     
         34 . The microbe of  claim 26 , wherein the genome comprises the DNA encoding a naturally occurring myo-2-inosose dehydratase.  
     
     
         35 . The microbe of  claim 27 , wherein the genome comprises the DNA encoding a naturally occurring inositol monophosphatase.  
     
     
         36 . A method for the production of 1,2,3,4-tetrahydroxybenzene, comprising: 
 a) incubating, in the presence of a carbon source, a microbe comprising a genome, a DNA encoding a naturally occurring myo-inositol-1-phosphate synthase, a DNA encoding a naturally occurring inositol dehydrogenase and a DNA encoding a naturally occurring myo-2-inosose dehydratase to produce D-2,3-diketo-4-deoxy-epi-inositol; and    b) converting the D-2,3-diketo-4-deoxy-epi-inositol to 1,2,3,4-tetrahydroxybenzene by acid catalyzed dehydration.    
     
     
         37 . The method of  claim 36 , wherein the DNA encoding the naturally occurring myo-inositol-1-phosphate synthase comprises an INO1 gene.  
     
     
         38 . The method of  claim 36 , wherein the INO1 gene comprises a  Saccharomyces cerevisiae  INO1.  
     
     
         39 . The method of  claim 36 , wherein the DNA encoding the naturally occurring inositol dehydrogenase comprises an iolG gene.  
     
     
         40 . The method of  claim 36 , wherein the DNA encoding the naturally occurring inositol dehydrogenase comprises a  Bacillus subtilis  iolG.  
     
     
         41 . The method of  claim 36 , wherein the DNA encoding the naturally occurring myo-inositol-1-phosphate synthase and the DNA encoding the naturally occurring inositol dehydrogenase are comprised by plasmid pAD2.28A.  
     
     
         42 . The method of  claim 36 , wherein the DNA encoding a naturally occurring myo-2-inosose dehydratase is a recombinant DNA encoding a naturally occurring myo-2-inosose dehydratase.  
     
     
         43 . The method of  claim 36 , wherein the microbe further comprises a DNA encoding a naturally occurring inositol monophosphatase.  
     
     
         44 . The method of  claim 43 , wherein the DNA encoding a naturally occurring inositol monophosphatase is a recombinant DNA encoding a naturally occurring inositol monophosphatase.  
     
     
         45 . The method of  claim 36 , wherein the DNA encoding a naturally occurring myo-inositol-1-phosphate synthase is a recombinant DNA encoding a naturally occurring myo-inositol-1-phosphate synthase.  
     
     
         46 . The method of  claim 36 , wherein the DNA encoding a naturally occurring inositol dehydrogenase is a recombinant DNA encoding a naturally occurring inositol dehydrogenase.  
     
     
         47 . The method of  claim 36 , wherein the microbe is an  Escherichia coli.    
     
     
         48 . The method of  claim 36 , wherein the  Escherichia coli  is  Escherichia coli  JWF1/pAD2.28A.  
     
     
         49 . The method of  claim 36 , wherein the genome comprises the DNA encoding a naturally occurring myo-inositol-1-phosphate synthase.  
     
     
         50 . The method of  claim 36 , wherein the genome comprises the DNA encoding a naturally occurring inositol dehydrogenase.  
     
     
         51 . The method of  claim 36 , wherein the genome comprises the DNA encoding a naturally occurring myo-2-inosose dehydratase.  
     
     
         52 . The method of  claim 43 , wherein the genome comprises the DNA encoding a naturally occurring inositol monophosphatase.  
     
     
         53 . The method of  claim 36 , wherein the carbon source comprises glucose.  
     
     
         54 . A fermentation composition comprising a microbe which comprises a recombinant DNA encoding a naturally occurring myo-inositol-1-phosphate synthase and a recombinant DNA encoding a naturally occurring inositol dehydrogenase.  
     
     
         55 . The fermentation composition of  claim 54 , wherein the DNA encoding the naturally occurring myo-inositol-1-phosphate synthase comprises an INO1 gene.  
     
     
         56 . The fermentation composition of  claim 55 , wherein the INO1 gene comprises a  Saccharomyces cerevisiae  INO1.  
     
     
         57 . The fermentation composition of  claim 54 , wherein the DNA encoding the naturally occurring inositol dehydrogenase comprises an iolG gene.  
     
     
         58 . The fermentation composition of  claim 54 , wherein the DNA encoding the naturally occurring inositol dehydrogenase comprises a  Bacillus subtilis  iolG.  
     
     
         59 . The fermentation composition of  claim 54 , wherein the DNA encoding the naturally occurring myo-inositol-1-phosphate synthase and the DNA encoding the naturally occurring inositol dehydrogenase are comprised by plasmid pAD2.28A.  
     
     
         60 . The fermentation composition of  claim 54 , wherein the microbe further comprises a DNA encoding a naturally occurring myo-2-inosose dehydratase.  
     
     
         61 . The fermentation composition of  claim 60 , wherein the DNA encoding a naturally occurring myo-2-inosose dehydratase is a recombinant DNA encoding a naturally occurring myo-2-inosose dehydratase.  
     
     
         62 . The fermentation composition of  claim 54 , wherein the microbe further comprises a DNA encoding a naturally occurring inositol monophosphatase.  
     
     
         63 . The fermentation composition of  claim 62 , wherein the DNA encoding a naturally occurring inositol monophosphatase is a recombinant DNA encoding a naturally occurring inositol monophosphatase.  
     
     
         64 . The fermentation composition of  claim 54 , wherein the microbe is an  Escherichia coli.    
     
     
         65 . The fermentation composition of  claim 54 , wherein the  Escherichia coli  is  Escherichia coli  JWF1/pAD2.28A.  
     
     
         66 . The fermentation composition of  claim 54 , wherein the genome comprises the DNA encoding a naturally occurring myo-inositol-1-phosphate synthase.  
     
     
         67 . The fermentation composition of  claim 54 , wherein the genome comprises the DNA encoding a naturally occurring inositol dehydrogenase.  
     
     
         68 . The fermentation composition of  claim 62 , wherein the genome comprises at least one recombinant DNA encoding a naturally occurring enzyme selected from the group consisting of inositol dehydrogenase, inositol monophosphatase and myo-2-inosose dehydratase.  
     
     
         69 . The fermentation composition of  claim 54 , wherein the carbon source comprises glucose.

Cited by (0)

No later patents cite this yet.

References (0)

No backward citations on record.