Method to detect IgE

Abstract

The present invention includes a method to detect IgE using a human Fc epsilon receptor (Fc ε R) to detect IgE antibodies in a biological sample from a cat dog, horse or mouse. The present invention also relates to kits to perform such methods.

106 . An isolated human Fc ε  receptor (Fc ε R) alpha chain protein, wherein a carbohydrate group of said Fc ε R alpha chain protein is conjugated to a detectable marker.  
     
     
         107 . The Fc ε R alpha chain protein of  claim 106 , wherein said detectable marker is selected from the group consisting of a radioactive label, a fluorescent label, a chemiluminescent label, a chromophoric label and a ligand.  
     
     
         108 . The Fc ε R alpha chain protein of  claim 106 , wherein said detectable marker is selected from the group consisting of fluorescein, a radioisotope, a phosphatase, biotin, biotin-related compounds, avidin, avidin-related compounds and a peroxidase.  
     
     
         109 . The Fc ε R alpha chain protein of  claim 106 , wherein said Fc ε R alpha chain protein is encoded by a nucleic acid molecule selected from the group consisting of a nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:10 and SEQ ID NO:12.  
     
     
         110 . The Fc ε R alpha chain protein of  claim 106 , wherein said Fc ε R alpha chain protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, SEQ ID NO:11, and SEQ ID NO:13.  
     
     
         111 . An isolated human Fc ε  receptor (Fc ε R) alpha chain protein, wherein a carbohydrate group of said Fc ε R alpha chain protein is conjugated to biotin.  
     
     
         112 . The Fc ε R alpha chain protein of claim  1  11, wherein said Fc ε R alpha chain protein is encoded by a nucleic acid molecule selected from the group consisting of a nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:10 and SEQ ID NO:12.  
     
     
         113 . The Fc ε R alpha chain protein of  claim 111 , wherein said Fc ε R alpha chain protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, SEQ ID NO:11, and SEQ ID NO:13.  
     
     
         114 . An isolated human Fc ε  receptor (Fc ε R) alpha chain protein, wherein a carbohydrate group of said Fc ε R alpha chain protein is conjugated to biotin, said protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, SEQ ID NO:11, and SEQ ID NO:13.  
     
     
         115 . The Fc ε R alpha chain protein of  claim 114 , wherein said Fc ε R alpha chain protein is encoded by a nucleic acid molecule selected from the group consisting of a nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:10 and SEQ ID NO:12.