Oligonucleotide specific to the species Escherichia coli and procedure for detecting and visualizing bacteria of this species

Abstract

The invention concerns a procedure for detecting and visualizing bacteria of the genomic species Escherichia coli, including all the Shigella genomnic species except for serotype 13 S. boydii/Escherichia fergusonii genomic species, with oligonucleotide capable of specifically hybridizing with the ribosomal RNA of the bacteria.

1 . Oligonucleotide capable of hybridizing specifically to the ribosomal RNA (rRNA) or to the corresponding gene (rDNA) of the genomic species  Escherichia coli  (including all the  Shigellea  with the exception of  S. boydii  serotype 13)/ Escherichia fergusonii.    
     
     
         2 . Oligonucleotide according to  claim 1  capable of hybridizing specifically to the 637-660 region of the 16S rRNA of  E coli.    
     
     
         3 . Oligonucleotide according to  claim 2  capable of hybridizing specifically with at least 10 nucleotides of the 637-660 region of the 16S rRNA of  E. coli.    
     
     
         4 . Oligonucleotide according to one of claims  1  to 3, characterized in that it corresponds to SEQ ID No. 1:  
     
     
         5 . Oligonucleotide according to one of  claims 1  to  3 , characterized in that it differs from a nucleotide of SEQ ID NO. 1.  
     
     
         6 . Oligonucleotide according to  claim 3 , characterized in that it corresponds to SEQ ID NO. 2.  
     
     
         7 . Complementary oligonucleotide of the oligo-nucleotide according to one of  claims 1  to  6 .  
     
     
         8 . Oligonucleotide according to one of  claims 1  to  7 , characterized in that it is labeled at its 3′ or 5′ end or at the 3′ and 5′ ends.  
     
     
         9 . Procedure for detection and visualization of bacteria of the genomic species  Escherichia coli  (including all the  Shigellae  with the exception of  S. boydii  serotype 13)/ Escherichia fergusonii  in a sample comprising a hybridization step of the ribosomal RNA of the bacteria of said genomic species with an oligonucleotide according to one of  claims 1  to  8 .  
     
     
         10 . Procedure according to  claim 9 , characterized in that the oligonucleotide is selected from SEQ ID NO. 1 and SEQ ID NO. 2.