Method for inducing an anti-tumor and anti-cachexia immune response in mammals

Abstract

The invention relates to inducing an immune response toward tumor associated antigens and in particular to the administration of high molecular weight isolates of autologous urine either alone, with adjuvants, or with antigen presenting cells. The antigen presenting cells have been cocultured with isolates of autologous urine. The invention can also be used to treat cachexia in cancer or AIDS patients.

What is claimed is:  
     
         1 . A method for treating cancer in a mammal, comprising: 
 collecting urine from a mammal with cancer;    preparing an isolate comprising a pool of molecules larger than about 1000 daltons from said urine; and    administering an effective amount of said isolate by injection into said mammal.    
     
     
         2 . The method of  claim 1  wherein said cancer is a solid tumor.  
     
     
         3 . The method of  claim 1  wherein said injection is selected from the group consisting of intra-muscular, intravenous, intradermal, subcutaneous, and intralymphatic.  
     
     
         4 . The method of  claim 1  wherein said injection is into the tumor itself.  
     
     
         5 . The method of  claim 1  wherein said mammal is a human.  
     
     
         6 . The method of  claim 1  wherein said preparation of said isolate comprises: 
 collecting about 1,000 to about 10,000 mls of urine from said mammal with cancer,  
 filtering the urine to remove particulate matter, and  
 concentrating the proteins larger than about 1,000 daltons.  
 
     
     
         7 . The method of  claim 6  wherein said concentrating comprises placing the filtered urine in a concentrator equipped with a 1,000 dalton filter cartridge.  
     
     
         8 . The method of  claim 1 , wherein said isolate comprises molecules larger than about 5,000 daltons.  
     
     
         9 . The method of  claim 1 , wherein said isolate comprises molecules larger than about 10,000 daltons.  
     
     
         10 . The method of  claim 10 , wherein said isolate comprises molecules smaller than about 60,000 daltons.  
     
     
         11 . The method of  claim 1 , wherein said isolate comprises molecules larger than about 100,000 daltons.  
     
     
         12 . The method of  claim 1 , wherein said isolate comprises molecules larger than about 1,000 and smaller than about 1,000,000 daltons.  
     
     
         13 . The method of  claim 1 , wherein said isolate comprises molecules larger than about 3,000 and smaller than about 100,000 daltons.  
     
     
         14 . The method of  claim 1 , wherein said isolate comprises molecules larger than about 10,000 and smaller than about 50,000 daltons.  
     
     
         15 . The method of  claim 1 , further comprising administering an effective amount of an immune-stimulating compound, selected from the group consisting of an adjuvant, a heat shock protein, and a bacterial cell wall extract.  
     
     
         16 . A method for treating cachexia in a mammal, comprising: 
 collecting urine from a mammal with cancer;    preparing an isolate comprising molecules larger than about 1,000 daltons from said urine; and    administering an effective amount of said isolate to a mammal with cachexia by injection.    
     
     
         17 . The method of  claim 16 , wherein said cancer is a solid tumor.  
     
     
         18 . The method of  claim 17 , wherein said injection is selected from the group consisting of intramuscular, intravenous, intradermal, subcutaneous, and intralymphatic.  
     
     
         19 . The method of  claim 16 , wherein said injection is into the tumor itself.  
     
     
         20 . The method of  claim 16  wherein said mammal is human.  
     
     
         21 . The method of  claim 16 , wherein said preparation of said isolate comprises: 
 collecting about 1,000 to about 10,000 mls of urine from said mammal with cancer,    filtering the urine to remove particulate matter, and    concentrating the proteins larger than about 1,000 daltons.    
     
     
         22 . The method of  claim 21 , wherein said concentrating comprises placing the filtered urine in a concentrator equipped with a 1,000 dalton filter cartridge.  
     
     
         23 . The method of  claim 16  wherein said cachexia is due to cancer or AIDS.  
     
     
         24 . The method of  claim 16 , wherein said isolate comprises molecules larger than about 10,000 daltons and smaller than about 100,000 daltons.  
     
     
         25 . The method of  claim 16 , wherein said isolate comprises molecules larger than about 10,000 daltons and smaller than about 40,000 daltons.  
     
     
         26 . The method of  claim 16 , further comprising administering an effective amount of an immune-stimulating compound, selected from a group consisting of an adjuvant, a heat shock protein, and a bacterial cell wall extract.  
     
     
         27 . The method of  claim 1 , further comprising: 
 pulsing antigen-presenting cells (APCs) with said isolate; and    reinfusing an effective amount of the APCs or exosomes from the APCs into said mammal with cancer.    
     
     
         28 . The method of  claim 27 , wherein said APCs are dendritic cells.  
     
     
         29 . The method of  claim 1 , wherein said isolate is administered within exosomes.  
     
     
         30 . The method of  claim 29 , wherein said exosomes are obtained from APCs which are co-cultured with said isolate.  
     
     
         31 . The method of  claim 30 , wherein the APCs are from the mammal being treated.  
     
     
         32 . The method of  claim 16 , further comprising: 
 pulsing antigen-presenting cells (APCs) with said isolate; and    reinfusing an effective amount of the APCs or exosomes from the APCs into the mammal with cancer.    
     
     
         33 . The method of  claim 32 , wherein said APCs are dendritic cells.  
     
     
         34 . The method of  claim 16 , wherein said isolate is administered within exosomes.  
     
     
         35 . The method of  claim 34 , wherein said exosomes are obtained from APCs which are co-cultured with said isolate.  
     
     
         36 . The method of  claim 27 , wherein said effective amount of APCs or exosomes are washed APCs or washed exosomes.  
     
     
         37 . A method for stimulating an anti-cancer immune response in a mammal with cancer, comprising: 
 collecting urine from said mammal with cancer;    preparing an isolate comprising a pool of molecules larger than about 1000 daltons from said urine; and    administering an effective amount of said isolate by injection into said mammal;    thereby stimulating an anti-cancer immune response in said mammal.