Process for preparing double-coated lactic acid bacteria powder using protein and polysaccharide and product by the same

Abstract

The present invention relates to a process for production of a double-coated lactic acid bacteria culture powders using protein and polysaccharide. The present invention can provide double-coated lactic acid bacteria through homogenization in the state of an aqueous solution and lyophilization process thereby requiring no additional equipment and apparatus. The double-coated lactic acid bacteria powders according to the present invention show improved heat-resistance, acid-resistance, bile-resistance and storage stability, as well as excellent survival rate until reaching the intestines

What is claimed is:  
     
         1 . A process for preparing a double-coated lactic acid bacteria culture powders comprising the steps of: 
 preparing a protein aqueous solution;    adding a proteolytic enzyme solution to the protein aqueous solution for proteolysis;    cultivating a lactic acid bacteria in the protein aqueous solution;    concentrating the lactic acid bacteria culture using a centrifuge wherein the lactic acid bacteria culture is coated with the protein;    introducing the lactic acid bacteria culture into a polysaccharide aqueous solution and mixing to form a homogenized solution; and    lyophilizing the homogenized solution.    
     
     
         2 . The process set forth in  claim 1 , wherein the polysaccharide is selected from the group consisting of Xantan gum, Cellulose, Levan and mixtures thereof.  
     
     
         3 . The process set forth in  claim 1 , wherein the protein aqueous solution comprises one selected from the group consisting of skim milk, isolated soybean protein and mixture thereof.  
     
     
         4 . The process set forth in  claim 1 , further comprising the step of mixing a glucose, an yeast extract, a concentrated meat extract and an ionic component into the protein aqueous solution before cultivating step.  
     
     
         5 . The process set forth in  claim 4 , wherein the ionic component is selected from the group consisting of ammonium citrate, sodium acetate, dipotassium phosphate, magnesium sulfate, manganese sulfate, sodium chloride and mixtures thereof.  
     
     
         6 . The process set forth in  claim 1 , wherein the polysaccharide aqueous solution further comprises a cryoprotectant.  
     
     
         7 . The process set forth in  claim 6 , wherein the cryoprotectant includes one selected from a group consisting of trehalose, malto-dextrin, mannitol, skim milk and mixtures thereof.  
     
     
         8 . A process for preparing a double-coated lactic acid bacteria culture powders comprising the steps of: 
 preparing a protein aqueous solution;    adding a proteolytic enzyme to the protein aqueous solution for proteolysis;    cultivating a lactic acid bacteria in the protein aqueous solution;    concentrating the lactic acid bacteria culture using a centrifuge wherein the lactic acid bacteria culture is coated with the protein;    mixing the lactic acid bacteria culture into a cryoprotectant aqueous solution homogeneously followed by lyophilization;    introducing the lyophilized lactic acid bacteria culture into a polysaccharide aqueous solution and mixing to form a homogenized solution; and    lyophilizing the homogenized solution.    
     
     
         9 . A double-coated lactic acid bacteria produced by the process described in any one of claims  1 - 8 .