Value-added traits in grain and seed transformed with thioredoxin

Abstract

Compositions and methods of use are provided herein to make and use transgenic plants with value-added traits.

1 - 31 . (Canceled).  
     
     
         32 . A transgenic monocot plant wherein at least part of said plant comprises a recombinant nucleic acid comprising a promoter active in said part operably linked to a nucleic acid molecule encoding a thioredoxin h polypeptide wherein said promoter is a seed or grain maturation-specific promoter.  
     
     
         33 . The transgenic plant of  claim 32  wherein said part is a seed.  
     
     
         34 . The transgenic plant of  claim 32  wherein said part is a grain.  
     
     
         35 . (Canceled).  
     
     
         36 . The transgenic plant of  claim 32  wherein said promoter is selected from the group consisting of rice glutelins, rice oryzins, rice prolamines, barley hordeins, wheat gliadins, wheat glutelins, maize zeins, maize glutelins, oat glutelins,  sorghum  kasirins, millet pennisetins, rye secalins, and a maize embryo-specific globulin promoter.  
     
     
         37 . The transgenic plant of  claim 36  wherein said barley hordein promoter is selected from the group consisting of 131 hordein and D hordein promoters.  
     
     
         38 . The transgenic plant of  claim 32  wherein said plant is selected from the group consisting of rice, barley, maize, wheat, oat, rye,  sorghum , millet, triticale, turf grass and forage grass.  
     
     
         39 . (Canceled).  
     
     
         40 . The transgenic plant of  claim 32  wherein said thioredoxin h is barley, wheat, tobacco, rice,  Brassica, Arabidopsis , Picea, or soy bean thioredoxin h.  
     
     
         41 . The transgenic plant of  claim 32  wherein said recombinant nucleic acid further comprises a nucleic acid molecule encoding a signal peptide operably linked to said promoter and said nucleic acid molecule encoding a thioredoxin protein.  
     
     
         42 . The transgenic plant of  claim 41  wherein said signal peptide targets expression of the thioredoxin polypeptide to an intracellular body.  
     
     
         43 . The transgenic plant of  claim 42  wherein said signal peptide is selected from the group consisting of barley B1 hordein and D hordein signal peptides.  
     
     
         44 - 76 . (Canceled).  
     
     
         77 . A transgenic seed or grain comprising a recombinant nucleic acid comprising a promoter active in said seed or grain operably linked to a nucleic acid molecule encoding thioredoxin h polypeptide wherein said promoter is a seed or grain maturation-specific promoter.  
     
     
         78 . (Canceled).  
     
     
         79 . The transgenic seed or grain of  claim 77  wherein said promoter is selected from the group 74 consisting of rice glutelins, rice oryzins, rice prolamines, barley hordeins, wheat gliadins, wheat glutelins, maize zeins, maize glutelins, oat glutelins,  sorghum  kasirins, millet pennisetins, rye secalins, and a maize embryo-specific globulin.  
     
     
         80 . The transgenic seed or grain of  claim 79  wherein said barley hordein promoter is selected from the group consisting of B1 hordein and D hordein promoters.  
     
     
         81 . The transgenic seed or grain of  claim 80  wherein said seed or grain is selected from the group consisting of rice, barley, maize, wheat, oat, rye,  sorghum , millet, and triticale seed or grain.  
     
     
         82 . (Canceled).  
     
     
         83 . The transgenic seed or grain of  claim 77  wherein said thioredoxin h is barley, wheat, tobacco, rice, soy bean,  Brassica , Picea, or  Arabidopsis  thioredoxin h.  
     
     
         84 . The transgenic seed or grain of  claim 77  wherein said recombinant nucleic acid further comprises a nucleic acid molecule encoding a signal peptide operably linked to said promoter and said nucleic acid molecule encoding a thioredoxin protein.  
     
     
         85 . The transgenic seed or grain of  claim 84  wherein said signal peptide targets expression of the thioredoxin polypeptide to an intracellular body.  
     
     
         86 . The transgenic seed or grain of  claim 85  wherein said signal peptide is selected from the group consisting of barley B1 hordein and D hordein signal peptides.  
     
     
         87 - 111 . (Canceled).  
     
     
         112 . The transgenic plant of  claim 32  wherein said plant is wheat.  
     
     
         113 . The transgenic plant of  claim 112  wherein said thioredoxin is wheat thioredoxin h.  
     
     
         114 . The transgenic seed or grain of  claim 81  wherein said seed or grain is barley.  
     
     
         115 . The transgenic seed or grain of  claim 114  wherein said thioredoxin is barley thioredoxin h.  
     
     
         116 . The transgenic seed or grain of  claim 81  wherein said seed or grain is wheat.  
     
     
         117 . The transgenic seed or grain of  claim 116  wherein said thioredoxin is wheat thioredoxin h.  
     
     
         118 . The transgenic plant of  claim 32  wherein said thioredoxin is  Arabidopsis thioredoxin h.  
     
     
         119 . The transgenic plant of  claim 32  wherein said thioredoxin is soybean thioredoxin h.  
     
     
         120 . The transgenic monocot seed or grain of  claim 77  wherein said thioredoxin is  Arabidopsis  thioredoxin h.  
     
     
         121 . The transgenic monocot seed or grain of  claim 77  wherein said thioredoxin is soybean thioredoxin h.  
     
     
         122 . The transgenic plant of  claim 32  wherein said thioredoxin is tobacco thioredoxin h.  
     
     
         123 . The transgenic plant of  claim 32  wherein said thioredoxin is  Brassica  thioredoxin h.  
     
     
         124 . The transgenic monocot seed or grain of  claim 77  wherein said thioredoxin is tobacco thioredoxin h.  
     
     
         125 . The transgenic monocot seed or grain of  claim 77  wherein said thioredoxin is  brassica  thioredoxin h.