US2005025787A1PendingUtilityA1
Transformed bacteria producing CS6 antigens as vaccines
Priority: May 13, 1994Filed: Jan 12, 2004Published: Feb 3, 2005
Est. expiryMay 13, 2014(expired)· nominal 20-yr term from priority
C07K 14/245A61P 37/04A61K 2039/523A61K 39/00
62
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Claims
Abstract
Disclosed herein are antigens that stimulate protective antibodies against enterotoxigenic Escherichia coli . Also disclosed herein are proteins encoded by cssA and cssB genes as well as constructs containing the genes and methods of using thereof.
Claims
exact text as granted — not AI-modified1 . A method of inducing, in a susceptible host, the production of antibodies against CS6 protein comprising administration of a composition of matter comprising a bacteria transformed with a plasmid which contains genes css A and css B, all of the css C and DNA sequence of css D which encodes at least 802 amino acids (at least 2406 base pairs), an origin of replication, a Lac promotor, and a kanamycin resistance gene wherein said bacteria expresses both CS6A and CS6B proteins.
2 . A method of claim 1 wherein the bacteria are in a pharmaceutically acceptable carrier.
3 . A method of claim 2 wherein the carrier is a carbonated liquid.
4 . A protein containing the amino acid sequence
RTEIATKNFPVSTTISKSFFA
PEPRIQPSFGENVGKEGALLF
SVNLTVPENVSQVTVYPVYDE
DYGLGRLVNTADASQSIIYQI
VDEKGKKMLKDHGAEVTPNQQ
ITFKALNYTSGEKKISPGIYN
DQVMVGYYVN.
5 . A composition of matter comprising a protein of claim 4 in a pharmaceutically acceptable carrier.
6 . A bacteria transformed with a plasmid which contains genes css A and css B, all of the css C and DNA sequence of css D which encodes at least 802 amino acids (at least 2406 base pairs), an origin of replication, a Lac promotor, and a kanamycin resistance gene wherein said bacteria expresses both CS6A and CS6B proteins.
7 . A composition of matter comprising bacteria of claim 6 in a pharmaceutically acceptable carrier.
8 . A composition of matter comprising the bacteria of claim 6 wherein the carrier is a carbonated beverage.
9 . A bacteria of claim 6 containing the sequence:
1
AAGCTTGTAA CCAGTTGATA AAAATATATC ACGCTGGGAA TGACGTGATG
(Sequence ID No #1)
51
TATATACGGA GCAGCTATGT CGGAACAGAT ATTTTCCTAT CGGTATGCGT
101
TGTGAGTAAG CGTAAAGCCA ATGCTGTCTG TAACTCCTGA TCCTTGCAGA
151
CTAAATTAGA GCTCCTTCTA AATTAGACGG ATGGATAAAC CTACAGACTG
201
GCGCTCTGGG TCTCGCCGGA TATTTTCTAA TGAATTTAAG CTTCATATGG
251
TTGAACTGGC TTCGAAACCA AATGCCAATG TCGCACAACT GGCTCGGGAA
301
CATGGCGTTG ATAACAACCT GATTTTTAAA TAGCTACGCC TCTGGCAAAG
351
AGAAGGACGT ATTTCTCGTA GAATGCCTCC AACTATTGTA GGCCCTACAG
401
TACCACTGAG GTAGCCTGAA TTTAAAGCCG AAGCGGTCAG AACTGTTCTT
451
GGTGTGAACG TAGCACTCAC CAATAAAAGC ATCAATACGG TGCTCTGTTG
501
ACACATTACG AATGTTATGT ATACAATAAA AATGATTATA GCAATATTAA
551
TGGTGTTATA TGAAGAAAAC AATTGGTTTA ATTCTAATTC TTGCTTCATT
601
CGGCAGCCAT GCCAGAACAG AAATAGCGAC TAAAAACTTC CCAGTATCAA
651
CGACTATTTC AAAAAGTTTT TTTGCACCTG AACCACGAAT ACAGCCTTCT
701
TTTGGTGAAA ATGTTGGAAA GGAAGGAGCT TTATTATTTA GTGTGAACTT
751
AACTGTTCCT GAAAATGTAT CCCAGGTAAC GGTCTACCCT GTTTATGATG
801
AAGATTATGG GTTAGGACGA CTAGTAAATA CCGCTGATGC TTCCCAATCA
851
ATAATCTACC AGATTGTTGA TGAGAAAGGG AAAAAAATGT TAAAAGATCA
901
TGGTGCAGAG GTTACACCTA ATCAACAAAT AACTTTTAAA GCGCTGAATT
951
ATACTAGCGG GGAAAAAAAA ATATCTCCTG GAATATATAA CGATCAGGTT
1001
ATGGTTGGTT ACTATGTAAA CTAAATACTG GAAGTATGAT TATGTTGAAA
1051
AAAATTATTT CGGCTATTGC ATTAATTGCA GGAACTTCCG GAGTGGTAAA
1101
TGCAGGAAAC TGGCAATATA AATCTCTGGA TGTAAATGTA AATATTGAGC
1151
AAAATTTTAT TCCAGATATT GATTCCGCTG TTCGTATAAT ACCTGTTAAT
1201
TACGATTCGG ACCCGAAACT GGATTCACAG TTATATACGG TTGAGATGAC
1251
GATCCCTGCA GGTGTAAGCG CAGTTAAAAT CGCACCAACA GATAGTCTGA
1301
CATCTTCTGG ACAGCAGATC GGAAAGCTGG TTAATGTAAA CAATCCAGAT
1351
CAAAATATGA ATTATTATAT CAGAAAGGAT TCTGGCGCTG GTAACTTTAT
1401
GGCAGGACAA AAAGGATCCT TTCCTGTCAA AGAGAATACG TCATACACAT
1451
TCTCAGCAAT TTATACTGGT GGCGAATACC CTAATAGCGG ATATTCGTCT
1501
GGTACTTATG CAGGAAATTT GACTGTATCA TTTTACAGCA ATTAAAAAAA
1551
GGCCGCATTA TTGCGGCCAT TGACGATACT GCTAGGCAAA AATATGAAAT
1601
CAAAGTTAAT TATACTATTG ACGTTAGTGC CATTTTCATC TTTTTCAACA
1651
GGAAATAATT TTGAAATAAA TAAGACACGA GTAATTTACT CTGACAGCAC
1701
ACCATCAGTT CAAATATCAA ATAATAAAGC ATATCCTTTA ATTATTCAAA
1751
GCAATGTATG GGATGAAAGC AATAATAAAA ATCATGACTT TATAGCAACA
1801
CCACCGATTT TTAAAATGGA AAGTGAAAGT CGGAATATAA TAAAAATAAT
1851
TAAAACAACT ATTAATTTGC CGGACTCTCA GGAAAGTATG AGATGGTTAT
1901
GTATTGAATC AATGCCACCA ATAGAAAAAA GTACTAAAAT AAACAGAAAA
1951
GAAGGAAGGA CAGACAGTAT TAATATCAGC ATTCGGGGGT GCATTAAACT
2001
GATATATCGA CCTGCCAGTG TTCCGTCTCC TGTTTTTAAT AATATAGTAG
2051
AAAAATTAAA ATGGCATAAA AATGGAAAGT ATCTTGTATT AAAAAATAAT
2101
ACACCCTATT ACATTAGCTT TTCTGAGGTT TTTTTTGATT CAGATAAAGT
2151
AAACAATGCA AAAGATATTT TATATGTAAA ACCATACTCA GAGAAGAAAA
2201
TAGATATCAG CAACAGAATA ATAAAAAAAA TCAAATGGGC TATGATTGAT
2251
GATGCTGGCG CAAAAACAAA ACTTTATGAA TCAATTTTAT AAAAAATCTC
2301
ATTACAGTAT ACAAAAACAT CAGATTACAG GCTTGCTTTT TTTGCTATTT
2351
ATATATCCTT TCTCAACCTC ATATGGAAAT GAACAATTTA GTTTTGACTC
2401
ACGATTCCTA CCATCAGGTT ATAATTACTC TTTAAATAGT AACTTACCTC
2451
CTGAAGGTGA GTATCTGGTT GATATTTATA TTAACAAAAT AAAAAAGGAG
2501
TCCGCGATTA TTCCTTTTTA TATAAAAGGA AATAAACTTG TACCATGTTT
2551
ATCAAAAGAA AAAATTTCAT CTTTGGGTAT CAACATTAAT AATAACGACA
2601
ACACAGAGTG TGTAGAAACA AGTAAGGCAG GTATTAGTAA TATCAGCTTT
2651
GAGTTTAGCT CTCTTCGTTT GTTTATTGCT GTACCGAAAA ATCTTCTGTC
2701
TGAGATTGAT AAAATATCAT CAAAGGATAT AGATAACGGG ATTCATGCTT
2751
TATTTTTTAA TTATCAAGTA AATACAAGGC TAGCCAATAA TAAAAATCGT
2801
TATGATTACA TTTCTGTTTC ACCAAATATA AATTATTTTT CATGGCGGTT
2851
GCGTAATCTT TTTGAATTTA ACCAAAACAA CGATGAAAAA ACATGGGAAA
2901
GAAACTACAC TTATCTAGAA AAAAGTTTTT ATGATAAAAA GCTAAACTTA
2951
GTCGTTGGTG AAAGTTATAC GAATTCAAAT GTTTATAATA ACTACTCTTT
3001
TACTGGTATT TCAGTTTCTA CAGATACAGA TATGTATACG CCAAGTGAAA
3051
TCGATTATAC ACCAGAAATT CATGGAGTGG CTGATTCAGA CTCTCAGATT
3101
ATTGTCAGGC AAGGCAACAC CATTATCATT AATGAAAGTG TTCCAGCCGG
3151
ACCGTTCTCA TTTCCAATAA CCAATCTCAT GTATACTGGG GGGCAACTTA
3201
ATGTGGAGAT AACAGATATT TATGGAAATA AAAAACAATA TACTGTCAAT
3251
AATTCCTCTC TTCCTGTTAT GAGAAAAGCG GGACTAATGG TATATAATTT
3301
TATATCTGGG AAATTAACAA AAAAAAATAG TGAGGATGGT GATTTTTTTA
3351
CTCAAGGTGA TATTAACTAC GGTACTCACT ATAACAGCAC ACTATTCGGT
3401
GGATATCAGT TTAGTAAAAA TTATTTTAAC TTATCTACTG GTATAGGCAC
3451
TGATCTGGGA TTTTCTGGAG CATGGCTACT ACACGTTAGC AGAAGTAATT
3501
TTAAGAATAA AAATGGATAT AATATTAATC TACAACAAAA CACTCAGTTA
3551
AGACCATTCA ATGCCGGGGT TAATTTCGAT TACGCATACA GAAAAAAAAG
3601
GTATGTGGAA CTTTCCGACA TTGGCTGGCA TGGTAATTTA TATAATCAAC
3651
TTAAAAATAG TTTTTCTTTA TCCTTGTCAA AATCATTGAA TAAATACGGA
3701
AATTTCTCAC TTGATTATAA CAAAATGAAA TACTGGGATA ATGCGTATGA
3751
TAGTAACTCA ATGTCGATTC GTTATTTTTT TAAATTCATG CGAGCAATGA
3801
TTACAACAAA TTGTTCTTTA AATAAATATC AATCTTATGA AAAAAAAGAT
3851
AAAAGATTTA GTATTAATAT ATCATTGCCT TTAACCAAAG ATTACGGGCA
3901
CATATCTTCA AACTATTCAT TTTCCAATGC AAATACACGA ACGGCAACCA
3951
GTTCTGTAGG CTTAAACGGT AGTTTTTTTA ATGACGCAAG ATTAAACTGG
4001
AACATTCAGC AGAACAGAAC GACCCGTAAC AATGGATATA CTGATAATAC
4051
CAGTTACATA GCAACCAGCT ATGCCTCTCC CTATGGCGTT TTTACTGGTT
4101
CATATTCAGG ATCGAACAAG TATTCAAGCC AGTTTTATTC TGCATCGGGA
4151
GGTATTGTTT TGCATAGCGA TGGCGTAGCT TTTACTCAAA AAGCCGGAGA
4201
TACCTCTGCT CTTGTCCGTA TTGATAATAT TTCTGATATA AAAATTGGTA
4251
ACACTCCTGG TGTTTATACT GGGTATAATG GTTTTGCTTT AATTCCTCAT
4301
CTTCAGCCGT TCAAAAAAAA CACCATTTTA ATTAATGATA AAGGAATTCC
4351
AGACGGTATT ACTCTTGCTA ATATAAAAAA ACAAGTTATC CCATCACGAG
4401
GAGCTATTGT TAAAGTAAAA TTTGATGCTA AAAAAGGCAA TGACATTTTG
4451
TTTAAGCTTA CAACTAAAGA TGGAAAAACG CCCCCATTAG GAGCTATAGC
4501
CCATGAAAAA AATGGAAAAC AGATTAATAC GGGTATCGTT GACGATGATG
4551
GTATGCTTTA TATGTCTGGA TTATCAGGGA CAGGGATTAT TAATGTAACA
4601
TGGAATGGAA AAGTCTGTTC ATTTCCTTTT TCAGAAAAAG ATATATCTAG
4651
CAAACAATTA TCTGTTGTAA ATAAACAATG TTAGGTAGTG CATCCAATTA
4701
GTAGAACATG TGTTTTTCGA TAAACGCTCC GATCTCTTTT TCGTGGATCT
4751
CAACTGAGCG TGAGAAGCAG ATTGTTTTAC GAGCCAACCG CTTAATGCGG
4801
GTGCGTAGCG TCAGATTATT ACGCTCAATG CGTTGGGTGA ATATTTTGCC
4851
GGTCAGATGC TTATTCTTCG GTACC
10 . A protein expressed by the bacteria of claim 6 containing the sequence:
RTEIATKNFPVSTTISKSFFA
(SEQ. ID No. 9)
PEPRIQPSFGENVGKEGALLF
SVNLTVPENVSQVTVYPVYDE
DYGLGRLVNTADASQSIIYQI
VDEKGKKMLKDHGAEVTPNQQ
ITFKALNYTSGEKKISPGIYN
DQVMVGYYVN.Join the waitlist — get patent alerts
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