Rupestris stem pitting associated virus nucleic acids, proteins, and their uses

Abstract

The present invention relates to an isolated protein or polypeptide corresponding to a protein or polypeptide of a Rupestris stem pitting associated virus. The encoding DNA molecule, either alone in isolated form, in an expression system, a host cell, or a transgenic grape plant, is also disclosed. Other aspects of the present invention relate to a method of imparting Rupestris stem pitting associated virus resistance to grape plants by transforming them with the DNA molecule of the present invention, and a method of detecting the presence of a Rupestris stem pitting associated virus, such as RSPaV-1, in a sample.

1 . An isolated protein or polypeptide corresponding to a protein or polypeptide of a  Rupestris  stem pitting associated virus.  
     
     
         2 - 17 . (cancelled)  
     
     
         18 . An isolated DNA molecule encoding a protein or polypeptide according to  claim 1 .  
     
     
         19 - 34 . (cancelled)  
     
     
         35 . A host cell transformed with a heterologous DNA molecule according to  claim 18 .  
     
     
         36 . The host cell according to  claim 35 , wherein the host cell is selected from a group consisting of  Agrobacterium vitis  and  Agrobacterium tumefaciens.    
     
     
         37 . The host cell according to  claim 35 , wherein the host cell is a grape cell.  
     
     
         38 . The host cell according to  claim 35 , wherein the protein or polypeptide is selected from a group consisting of a replicase, a coat protein, and a protein of a triple gene block.  
     
     
         39 . A transgenic  Vitis  scion cultivar or rootstock cultivar comprising the DNA molecule according to  claim 18 .  
     
     
         40 . A transgenic  Vitis  scion cultivar or rootstock cultivar according to  claim 39 , wherein the protein or polypeptide is selected from a group consisting of a replicase, a coat protein, and a protein of a triple gene block.  
     
     
         41 - 63 . (cancelled).