Arrangement for the detection of illumination radiation in a laser scanning microscope

Abstract

An arrangement for the detection of the illumination radiation in a laser scanning microscope, wherein a portion of the illumination radiation is coupled out and detected at the main color splitter, wherein light transmitted through the main color splitter is advantageously detected and/or a portion of the illumination radiation is coupled out and detected before coupling into a light-conducting fiber and/or a portion of the illumination radiation is coupled out and detected at a beam splitter arranged downstream of a light-conducting fiber.

1 - 9 . (cancelled).  
   
   
       10 . An arrangement for the detection of the illumination radiation in a laser scanning microscope, comprising that a portion of the illumination radiation is coupled out and detected at the main color splitter.  
   
   
       11 . The arrangement according to  claim 10 , wherein light transmitted through the main color splitter is detected.  
   
   
       12 . An arrangement for the detection of the illumination radiation in a laser scanning microscope, wherein a portion of the illumination radiation is coupled out and detected before coupling into a light-conducting fiber.  
   
   
       13 . The arrangement according to  claim 12 , wherein coupling out is carried out behind an AOTF arranged in the illumination beam path.  
   
   
       14 . An arrangement for the detection of the illumination radiation in a laser scanning microscope, comprising that a portion of the illumination radiation is coupled out and detected at a beam splitter arranged downstream of the light-conducting fiber.  
   
   
       15 . The arrangement according to  claim 14 , wherein light transmitted through the input-coupling splitter is detected.  
   
   
       16 . A method for the operation of an arrangement according to  claim 10 , comprising the step of carrying out a measurement during a continuous AOTF mode.  
   
   
       17 . The method for the operation of an arrangement according to  claim 10 , comprising the step of carrying out the measurement so as to be synchronous to the line-by-line detection of the sample.  
   
   
       18 . The method for the operation of an arrangement according to  claim 10 , comprising the step of carrying out a measurement with a plurality of receivers for diagnosing intensity variations and/or misalignment.