Two human heat shock protein homolgs

Abstract

The invention provides two human heat shock protein homologs (HSPHH-1, HSPHH-2) and polynucleotides which identify and encode HSPHH-1 and HSPHH-2. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention also provides methods for treating disorders associated with expression of HSPHH-1 and HSPHH-2.

1 . A substantially purified human heat shock protein homolog (HSPHH) comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, a fragment of SEQ ID NO:1, and a fragment of SEQ ID NO:3.  
     
     
         2 . A substantially purified variant of HSPHH having at least 90% amino acid identity to the amino acid sequence of  claim 1 .  
     
     
         3 . An isolated and purified polynucleotide sequence encoding the HSPHH of  claim 1 .  
     
     
         4 . An isolated and purified polynucleotide variant having at least 90% polynucleotide identity to the polynucleotide sequence of  claim 3 .  
     
     
         5 . A composition comprising the polynucleotide sequence of  claim 3 .  
     
     
         6 . An isolated and purified polynucleotide sequence which hybridizes under stringent conditions to the polynucleotide sequence of  claim 3 .  
     
     
         7 . An isolated and purified polynucleotide sequence which is complementary to the polynucleotide sequence of  claim 3 .  
     
     
         8 . An isolated and purified polynucleotide sequence comprising a polynucleotide sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, a fragment of SEQ ID NO:2, and a fragment of SEQ ID NO:4.  
     
     
         9 . An isolated and purified polynucleotide variant having at least 90% polynucleotide identity to the polynucleotide sequence of  claim 8 .  
     
     
         10 . An isolated and purified polynucleotide sequence which is complementary to the polynucleotide sequence of  claim 8 .  
     
     
         11 . An expression vector containing at least a fragment of the polynucleotide sequence of  claim 3 .  
     
     
         12 . A host cell containing the expression vector of  claim 11 .  
     
     
         13 . A method for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:1, SEQ ID NO:3, a fragment of SEQ ID NO:1, or a fragment of SEQ ID NO:3, the method comprising the steps of: 
 a) culturing the host cell of  claim 12  under conditions suitable for the expression of the polypeptide; and    b) recovering the polypeptide from the host cell culture.    
     
     
         14 . A pharmaceutical composition comprising the HSPHH of  claim 1  in conjunction with a suitable pharmaceutical carrier.  
     
     
         15 . A purified antibody which specifically binds to the HSPHH of  claim 1 .  
     
     
         16 . A purified agonist of the HSPHH of  claim 1 .  
     
     
         17 . A purified antagonist of the HSPHH of  claim 1 .  
     
     
         18 . A method for treating or preventing cancer, the method comprising administering to a subject in need of such treatment an effective amount of the antagonist of  claim 17 .  
     
     
         19 . A method for treating or preventing inflammation, the method comprising administering to a subject in need of such treatment an effective amount of the antagonist of  claim 17 .  
     
     
         20 . A method for detecting a polynucleotide encoding HSPHH in a biological sample, the method comprising the steps of: 
 (a) hybridizing the polynucleotide of  claim 7  to nucleic acids in the biological sample, thereby forming a hybridization complex; and    (b) detecting the hybridization complex, wherein the presence of the hybridization complex correlates with the presence of a polynucleotide encoding HSPHH in the biological sample.    
     
     
         21 . The method of  claim 20  wherein the nucleic acids are amplified by the polymerase chain reaction prior to hybridization.