Methods and compositions for detecting signals in binding assays

Abstract

Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the presently claimed invention provides a method of labeling a hybridized target wherein the target comprises a binding ligand. In another embodiment, the presently claimed invention provides methods of signal amplification.

1 - 14 . (cancelled)  
     
     
         15 . A complex comprising: a probe immobilized to a solid substrate; a target molecule hybridized to said probe, said target molecule comprising a binding ligand; and a receptor complexed to said binding ligand, said receptor being conjugated to a detectable microparticle.  
     
     
         16 . The complex of  claim 15  wherein said binding ligand is a biotin and said receptor is streptavidin.  
     
     
         17 . The complex of  claim 15  wherein said microparticle is fluorescently dyed.  
     
     
         18 . A complex comprising: a probe immobilized to a solid substrate; a target molecule hybridized to said probe, said target molecule comprising a first binding ligand; a first receptor complexed to said first binding ligand; an anti-receptor complexed to said first receptor, said anti-receptor comprising a plurality of second binding ligands and being conjugated to a microparticle; and a second receptor complexed to said anti-receptor, wherein said second receptor comprises a detectable moiety.  
     
     
         19 - 22 . (cancelled)