Osteoclast proton pump subunit

Abstract

Novel gene encoding a human 116-kDa osteoclast proton pump subunit and the protein encoded thereby. Methods of treatment of bone mass disorders utilizing said gene, and antibodies that bind to the protein encoded by said gene.

1 . An isolated gene encoding a polypeptide which is a human 116-kDa osteoclast proton pump subunit.  
     
     
         2 . The gene according to  claim 1 , wherein the gene comprises a nucleotide sequence consisting of SEQ ID NO: 1.  
     
     
         3 . An isolated DNA encoding a polypeptide which is an osteoclast 116-kDa proton pump subunit and comprising the nucleotide sequence of SEQ ID NO: 1 or its complementary nucleotide sequence.  
     
     
         4 . An isolated DNA encoding a polypeptide which is a human osteoclast proton pump subunit and which comprises the amino acid sequence of SEQ ID NO: 3.  
     
     
         5 . An isolated DNA encoding a polypeptide which is an osteoclast proton pump subunit, comprising a nucleotide sequence selected from the group consisting of: 
 a) SEQ ID NO: 1 or its complementary nucleotide sequence; and    b) nucleotide sequences which hybridize under conditions of medium stringency to the nucleotide sequences of (a).    
     
     
         6 - 16 . (Cancelled)  
     
     
         17 . An isolated polynucleotide comprising a sequence encoding a human 116-kDa osteoclast proton pump subunit polypeptide or a fragment thereof.  
     
     
         18 . The polynucleotide of  claim 17 , wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:2.  
     
     
         19 . The polynucleotide of  claim 17 , wherein said fragment comprises the amino acid sequence of SEQ ID NO:4.  
     
     
         20 . The polynucleotide of  claim 17 , wherein said fragment comprises the amino acid sequence of SEQ ID NO:5.  
     
     
         21 . The polynucleotide of  claim 17 , wherein said fragment comprises the amino acid sequence of SEQ ID NO:6.  
     
     
         22 . The polynucleotide of  claim 17 , wherein said fragment comprises the amino acid sequence of SEQ ID NO:7.  
     
     
         23 . The polynucleotide of  claim 17 , wherein said fragment comprises the amino acid sequence of SEQ ID NO:8.  
     
     
         24 . The polynucleotide of  claim 17 , wherein said fragment comprises the amino acid sequence of SEQ ID NO:9.  
     
     
         25 . The polynucleotide of  claim 17 , wherein said polynucleotide comprises the sequence of SEQ ID NO: 1 or the complement thereof.  
     
     
         26 . An isolated polynucleotide comprising a sequence which hybridizes at high stringency to the polynucleotide of  claim 17 .  
     
     
         27 . An isolated polynucleotide comprising a sequence which hybridizes at medium stringency to the polynucleotide of  claim 17 .  
     
     
         28 . An isolated polynucleotide comprising a sequence which hybridizes at high stringency to the polynucleotide of  claim 25 .  
     
     
         29 . An isolated polynucleotide comprising a sequence which hybridizes at medium stringency to the polynucleotide of  claim 25 .  
     
     
         30 . The polynucleotide of  claim 17 , wherein said polynucleotide is operably linked to a regulatory sequence.  
     
     
         31 . The polynucleotide of  claim 25 , wherein said polynucleotide is operably linked to a regulatory sequence.  
     
     
         32 . A cell comprising the polynucleotide of  claim 17 .  
     
     
         33 . A cell comprising the polynucleotide of  claim 25.