Method for producing fermented milk containing angiotensin converting enzyme inhibitory peptide and method for producing whey

Abstract

There are disclosed methods for producing fermented milk and whey that enable effective production in high yield of fermented milk and whey having high content of an ACEI peptide that is highly safe and applicable to pharmaceuticals, functional foods, health foods, and the like. The methods are: a method including the steps of mixing lactic acid bacteria and a starting material containing milk by stirring to prepare a mixed material, and fermenting the mixed material under stirring so that curd pieces and whey containing an angiotensin converting enzyme inhibitory peptide are generated, whereby fermented milk containing the curd pieces and the whey containing the angiotensin converting enzyme inhibitory peptide is produced; and a method including the steps of subjecting the resulting fermented milk to centrifugation and/or filter pressing to separate and recover whey.

1 . A method for producing fermented milk containing an angiotensin converting enzyme inhibitory peptide comprising: 
 (A) mixing lactic acid bacteria and a starting material containing milk by stirring to prepare a mixed material, and    (B-1) fermenting said mixed material under stirring so that curd pieces and whey containing an angiotensin converting enzyme inhibitory peptide are generated,    whereby fermented milk containing said curd pieces and said whey containing the angiotensin converting enzyme inhibitory peptide is produced.    
     
     
         2 . The method of  claim 1  wherein said milk is selected from the group consisting of cow's milk, goat's milk, sheep's milk, soy bean milk, skim milk, reconstituted milk, powdered mil, condensed milk and mixtures thereof.  
     
     
         3 . The method of  claim 1  wherein said fermented milk has a viscosity of not higher than 20 cp.  
     
     
         4 . The method of  claim 1  wherein said angiotensin converting enzyme inhibitory peptide is selected from the group consisting of Val-Pro-Pro, Ile-Pro-Pro, and mixtures thereof.  
     
     
         5 . The method of  claim 1  wherein said mixed material further contains a yeast.  
     
     
         6 . The method of  claim 1  wherein said lactic acid bacteria contained in the mixed material comprises  Lactobacillus helveticus.    
     
     
         7 . The method of  claim 6  wherein the  Lactobacillus helveticus  comprises  Lactobacillus helveticus  CM4 (NATIONAL INSTITUTE OF BIOSCIENCE AND HUMAN TECHNOLOGY, AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY, Deposit No. FERM BP-6060, Deposit date: Aug. 15, 1997).  
     
     
         8 . A method for producing whey containing an angiotensin converting enzyme inhibitory peptide comprising: 
 subjecting the fermented milk produced by the method of  claim 1  to at least one of centrifugation and filter pressing to separate and recover whey.    
     
     
         9 . A method for producing fermented milk containing an angiotensin converting enzyme inhibitory peptide comprising: 
 (A) mixing lactic acid bacteria and a starting material containing milk by stirring to prepare a mixed material,    (B-1) fermenting said mixed material under stirring so that curd pieces and whey containing an angiotensin converting enzyme inhibitory peptide are generated, and    (B-2) fermenting said mixed material under static conditions,    whereby fermented milk containing said curd pieces and said whey containing the angiotensin converting enzyme inhibitory peptide is produced.    
     
     
         10 . The method of  claim 9  wherein said milk is selected from the group consisting of cow's milk, goat's milk, sheep's milk, soy bean milk, skim milk, reconstituted milk, powdered milk, condensed milk and mixtures thereof.  
     
     
         11 . The method of  claim 9  wherein said fermented milk has a viscosity of not higher than 20 cp.  
     
     
         12 . The method of  claim 9  wherein said angiotensin converting enzyme inhibitory peptide is selected from the group consisting of Val-Pro-Pro, Ile-Pro-Pro, and mixtures thereof.  
     
     
         13 . The method of  claim 9  wherein said mixed material further contains a yeast.  
     
     
         14 . The method of  claim 9  wherein said lactic acid bacteria contained in the mixed material comprises  Lactobacillus helveticus.    
     
     
         15 . The method of  claim 14  wherein said  Lactobacillus helveticus  comprises  Lactobacillus helveticus  CM4 (NATIONAL INSTITUTE OF BIOSCIENCE AND HUMAN TECHNOLOGY, AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY, Deposit No. FERM BP-6060, Deposit date: Aug. 15, 1997).  
     
     
         16 . A method for producing whey containing an angiotensin converting enzyme inhibitory peptide comprising: 
 subjecting the fermented milk produced by the method of  claim 9  to at least one of centrifugation and filter pressing to separate and recover whey.