Modified enzymes having polymer conjugates

Abstract

The present invention relates to polypeptide-polymer conjugates having added and/or removed one or more attachment groups for coupling polymeric molecules on the surface of the polypeptide structure, a method for preparing polypeptide-polymer conjugates of the invention, the use of said conjugated for reducing the immunogenicity and allergenicity and compositions comprising said conjugate.

1 - 35 . (canceled)  
     
     
         36 . A conjugate comprising a lipase moiety conjugated to one or more polymers, wherein the lipase moiety is a  Humicola lanuginosa  lipase which comprises one or more of the following substitutions: 
 A18K, G31K, T32K, N33K, G38K, A40K, D48K, T50K, E56K, D57K, S58K, G59K, V60K, G61K, D62K, T64K, L78K, N88K, G91K, N92K, L93K, S105K, G106K, R118K, V120K, R125K, R133K, P136K, R139K, R160K, R179K, R209K, G225K, L227K, V228K, P229K, P250K and F262K.    
     
     
         37 . The conjugate of  claim 36 , wherein the  Humicola lanuginosa  lipase has an amino acid sequence of SEQ ID NO: 6.  
     
     
         38 . The conjugate of  claim 36 , wherein the polymer(s) have a molecular weight from 1 to 60 kDa.  
     
     
         39 . The conjugate of  claim 36 , wherein the polymer(s) are natural or synthetic homo or heteropolymers.  
     
     
         40 . The conjugate of  claim 36 , wherein the polymer(s) are selected from the group consisting of polyols, polyamines, polycarboxyl acids and polymers comprising a hydroxyl group and an amine group.  
     
     
         41 . The conjugate of  claim 36 , wherein the polymer(s) are selected from the group consisting of polyalkylene oxides (PAO), PEG-glycidyl ethers (Epox-PEG), PEG-oxycarbonylimidazole (CDI-PEG), branched PEGs, polyvinyl alcohols (PVA), poly-carboxylates, polyvinylpyrolidones, poly-D,L-amino acids, polyethylene-co-maleic acid anhydride, polystyrene-co-malic acid anhydrides, dextrans, heparins, homologous albumins, celluloses, hydrolysates of chitosan, starches, glycogen, agaroses and derivatives thereof, guar gum, pullulan, inulin, xanthan gum, carrageenin, pectin, alginic acid hydrolysates and bio-polymers.  
     
     
         42 . The conjugate of  claim 36 , wherein the polymer(s) are polyalkylene glycols (PAG) or methoxypolyethylene glycols (mPEG).  
     
     
         43 . The conjugate of  claim 36 , wherein the polymer(s) are selected from the group consisting of polyethylene glycols (PEG), polypropylene glycols and carboxymethyl-dextrans.  
     
     
         44 . The conjugate of  claim 36 , wherein the polymer(s) are selected from the group consisting of methylcellulose, carboxymethylcellulose, ethylcellulose, hydroxyethylcellulose carboxyethylcellulose and hydroxypropylcellulose.  
     
     
         45 . The conjugate of  claim 36 , wherein the polymer(s) are hydroxyethyl-starches or hydroxypropyl-starches.  
     
     
         46 . The conjugate of  claim 36 , wherein the polymer(s) are methoxypolyethylene glycols (mPEG).  
     
     
         47 . A detergent composition comprising a conjugate of  claim 36  and a surfactant.  
     
     
         48 . A skin care composition, comprising a conjugate of  claim 36  and ingredients used in skin care products.  
     
     
         49 . A pharmaceutical composition comprising a conjugate of  claim 36  and further comprising ingredients used in pharmaceuticals.