Aspergillus fumigatus profilin

Abstract

The invention provides isolated nucleic acid and amino acid sequences of A. fumigatus profilin, methods of screening for A. fumigatus profilin modulators using biologically active A. fumigatus profilin, and kits for screening for A. fumigatus profilin modulators.

1 . An isolated nucleic acid sequence encoding  A. fumigatus  profiling protein, wherein the protein has the following properties: (i) the protein's activity includes binding to actin; and (ii) the protein has a sequence that has greater than 90% amino acid sequence identity to SEQ ID NO:2 as measured using a sequence comparison algorithm.  
     
     
         2 . An isolated nucleic acid sequence of  claim 1 , wherein the protein specifically binds to polyclonal antibodies to a protein comprising SEQ ID NO:2.  
     
     
         3 . An isolated nucleic acid sequence of  claim 1 , wherein the nucleic acid encodes SEQ ID NO:2.  
     
     
         4 . An isolated nucleic acid sequence of  claim 1 , wherein the nucleic acid has a nucleotide sequence of SEQ ID NO:1.  
     
     
         5 . An isolated nucleic acid sequence of  claim 1 , wherein the nucleic acid selectively hybridizes under stringent hybridization conditions to SEQ ID NO:1.  
     
     
         6 . An expression vector comprising a nucleic acid encoding a  Aspergillus fumigatus  profilin protein, wherein the protein has the following properties: (i) the protein's activity includes binding to actin; and (ii) the protein has a sequence that has greater than 90% amino acid sequence identity to SEQ ID NO:2 as measured using a sequence comparison algorithm.  
     
     
         7 . A host cell transfected with the vector of  claim 6 .  
     
     
         8 . An isolated  Aspergillus fumigatus  profiling protein, wherein the protein has greater than 90% amino acid sequence identity to SEQ ID NO:2 as measured using a sequence comparison algorithm.  
     
     
         9 . An isolated protein of  claim 8 , wherein the protein specifically binds to polyclonal antibodies to  A. fumigatus  profilin.  
     
     
         10 . An isolated protein of  claim 8 , wherein the protein is  A. fumigatus  profilin.  
     
     
         11 . An isolated protein of  claim 8 , wherein the protein has an amino acid sequence of SEQ ID NO:2.  
     
     
         12 . An isolated nucleic acid comprising a sequence which has greater than 80% sequence identity with nucleotide SEQ ID NO:1.