US2005087452A1PendingUtilityA1
Antioxidant sensor, methods and compositions
Est. expiryAug 26, 2023(expired)· nominal 20-yr term from priority
A61P 39/06A61K 31/7048A61K 36/82A61K 31/353A61K 36/24G01N 33/1806Y10T436/142222A61K 36/87A61K 31/355Y10S436/903Y10S435/882A61P 39/00B01D 53/62A61K 31/375C12Q 1/37G01N 27/49G01N 21/17G01N 33/82G01N 33/68G01F 1/64A61K 31/352
49
PatentIndex Score
0
Cited by
0
References
0
Claims
Abstract
The present invention includes an apparatus and method for detecting directly the antioxidant activity of both lipophilic and lipophobic anti-oxidants that uses an oxygen radical sensitive sensor in fluid communication with a sample in a solvent/water/surfactant mixture; wherein the oxygen radical sensitive sensor concurrently detects both lipophilic and lipophobic anti-oxidants in the solvent/water/surfactant mixture.
Claims
exact text as granted — not AI-modified1 . An apparatus for detecting directly the antioxidant activity of both lipophilic and lipophobic anti-oxidants comprising:
an oxygen sensor in fluid communication with a sample and an oxygen radical sensitive molecule in a solvent/water/surfactant mixture; wherein the oxygen radical sensitive sensor concurrently detects both lipophilic and lipophobic anti-oxidants in the solvent/water/surfactant mixture.
2 . The apparatus of claim 1 , wherein the wherein the oxygen radical sensitive molecule is selected from molecules that react with oxygen, molecules with conjugated double bonds; Nitrogen or Sulfur containing compounds, fluorescein, β-PE, glutathione-S-transferase, linoleic acid or combinations thereof.
3 . The apparatus of claim 1 , wherein a dissolved oxygen level is determined using a dissolved oxygen meter.
4 . The apparatus of claim 1 , wherein a dissolved oxygen level is determined using an oxygen sensor comprising a electrochemical, a chemiluminescent, a surface plasmon resonance, an infrared, a capacitance coupled, a dye-coupled fiber optic or a hyperspectral oxygen sensor.
5 . The apparatus of claim 1 , wherein the solvent is defined further as an organic solvent.
6 . The apparatus of claim 1 , wherein the surfactant is defined further as a detergent.
7 . The apparatus of claim 1 , wherein the surfactant is defined further as a non-ionic detergent.
8 . The apparatus of claim 1 , wherein the solvent in the solvent/water/surfactant mixture is at least 10 percent in volume of the solvent/water/surfactant mixture.
9 . The apparatus of claim 1 , wherein the water in the solvent/water/surfactant mixture is at least 10 percent in volume of the solvent/water/surfactant mixture.
10 . The apparatus of claim 1 , wherein the surfactant in the solvent/water/surfactant mixture is at least 10 percent in volume of the solvent/water/surfactant mixture.
11 . The apparatus of claim 1 , wherein the solvent/water/surfactant ratio is about 1:1:1.
12 . The apparatus of claim 1 , wherein the solvent comprises acetone.
13 . The apparatus of claim 1 , wherein the surfactant comprises Tween.
14 . The apparatus of claim 1 , wherein the oxygen radical level is determined using the formula:
ORAC
(
o
)
=
AUC
SMP
-
AUC
BLNK
AUC
TRLX
-
AUC
BLNK
×
1000
(
mg
/
gr
)
×
[
TRLX
(
μmol
/
ml
)
]
[
SMP
(
mg
/
ml
)
]
wherein AUCsmp is the area under the curve value of the sample;
wherein AUCblnk is the area under the curve value of the blank;
wherein AUCtrlx is the area under the curve value for Trolox; and
wherein SMP is the sample.
15 . A method of determining anti-oxidant activity comprising the steps of:
determining a dissolved oxygen level in a test solution dissolved in a solvent/water/surfactant mixture in the presence of one or more anti-oxidants and an oxygen radical target, wherein both aqueous and lipid soluble anti-oxidant activity is measured with an oxygen detector.
16 . The method of claim 15 , wherein the dissolved oxygen level is determined using an oxygen detector comprising electrochemical, chemiluminescent, surface plasmon resonance, capacitance coupled, a dye-coupled fiber optic or a hyperspectral oxygen sensor.
17 . The method of claim 15 , wherein the dissolved oxygen level is determined using a dissolved oxygen sensor.
18 . The method of claim 15 , wherein the oxygen radical level is determined using the formula:
ORAC
(
o
)
=
AUC
SMP
-
AUC
BLNK
AUC
TRLX
-
AUC
BLNK
×
1000
(
mg
/
gr
)
×
[
TRLX
(
μmol
/
ml
)
]
[
SMP
(
mg
/
ml
)
]
wherein AUCsmp is the area under the curve value of the sample;
wherein AUCblnk is the area under the curve value of the blank;
wherein AUCtrlx is the area under the curve value for Trolox; and
wherein SMP is the sample.
19 . The method of claim 15 , wherein the solvent is defined further as an organic solvent.
20 . The method of claim 15 , wherein the surfactant is defined further as a detergent.
21 . The method of claim 15 , wherein the surfactant is defined further as a non-ionic detergent.
22 . The method of claim 15 , wherein the solvent in the solvent/water/surfactant mixture is at least 10 percent in volume of the solvent/water/surfactant mixture.
23 . The method of claim 15 , wherein the water in the solvent/water/surfactant mixture is at least 10 percent in volume of the solvent/water/surfactant mixture.
24 . The method of claim 15 , wherein the surfactant in the solvent/water/surfactant mixture is at least 10 percent in volume of the solvent/water/surfactant mixture.
25 . The method of claim 15 , wherein the solvent/water/surfactant ratio is about 1:1:1.
26 . The method of claim 15 , wherein the solvent comprises acetone.
27 . The method of claim 15 , wherein the surfactant comprises an anionic detergent, an ionic detergent or mixtures thereof.
28 . The method of claim 15 , wherein the surfactant comprises Tween.
29 . The method of claim 15 , wherein the anti-oxidant activity is measured at about 37 degrees Centrigrade.
30 . The method of claim 15 , further comprising the step of providing a radical intiator selected from the group consisting of 2,2′-azobis[2-(5-methyl-2-imidazolin-2-yl)propane]dihydrochloride, 2,2′ azobis (2-amidinopropane)dihydrochloride (AAPH), 2,2′-azobis(2-amidinopropane)[2-(N-stearyl)amidinopropane]dihydrochloride (SA-1), 2,2′-azo(2-(2-imidiazolin-2-yl)-propane)-[2-[2-(4-n-octyl)imidazolin-2-yl]-propane]dihydrochloride (C-8), 2,2′-azobis(4-methoxy-2,4-dimethylvaleronitrile) (MeO-AMVN), 2,2′-azobis(2,4-dimethylvaleronitrile) (AMVN), azo-bis-isobutylnitrile, 2,2′-azobis (2-methylproprionate) (DAMP), and 2,2′-azobis-(2-amidinopropane), salts, mixtures or equivalents thereof.
32 . The method of claim 15 , wherein the detector is disposable.
33 . An antioxidant dietary supplement comprising:
an isolated and purified lipophobic anti-oxidant; and an isolated and purified lipophilic anti-oxidant, wherein the lipophobic and the lipophilic antioxidants combined have a dissolved oxygen value of greater than 6,000 μMol Trolox Equivalents (TE)/gram.
34 . The supplement of claim 33 , wherein the lipophobic and the lipophilic anti-oxidant are time-released.
35 . The supplement of claim 33 , wherein the lipophobic anti-oxidant is selected from the group consisting of one or more vitamin E selected from the group consisting of alpha, beta, delta, epsilon, gamma, zeta, eta, xi1, xi2, and sigma tocopherols, and alpha, beta, delta and gamma tocotrienols, analogs thereof, pharmaceutically acceptable salts thereof, and combinations thereof.
36 . The supplement of claim 33 , wherein the lipophilic anti-oxidant is selected from the group consisting of quercetin, kaempferol, myricetin, apigenin and derivates, analogs, pharmaceutically acceptable salts thereof, and combinations thereof.
37 . The supplement of claim 33 , further comprising two or more essential saccharides.
38 . The supplement of claim 33 , further comprising two or more saccharides selected from galactose, galactosamine, glucosamine, glucose, mannose, acetylated-mannose, N-acetylneuraminic acid, fucose, N-acetylgalactosamine, N-acetylglucosamine and xylose.
39 . The supplement of claim 33 , further comprising a plant source of vitamin C.
40 . The supplement of claim 39 , wherein the plant source of vitamin C comprises an Australian bush plum ( Terminalia ferdinandiana ).
41 . The supplement of claim 39 , wherein the plant source of vitamin C comprises a wild-Australian bush plum ( Terminalia ferdinandiana ).
42 . The supplement of claim 39 , further comprising one or more pro-biotics.
43 . The supplement of claim 39 , further comprising one or more pro-biotics selected from Lactobacillus sp. and Bifidobacterium sp.
44 . The supplement of claim 33 , wherein the supplement is compressed to provide a generally oxygen impermeable surface.
45 . The supplement of claim 33 , wherein the supplement is a roller-compressed particle, a capsule, a tablet, a mini-tab, a caplet, an effervescent tablet or combinations thereof.
46 . The supplement of claim 33 , wherein the isolated and purified lipophilic anti-oxidant, wherein the lipophobic and the lipophilic antioxidants have an anti-oxidant ORAC(fl-lipo) value greater than 7000 μMol Trolox Equivalents (TE)/gram.
47 . The supplement of claim 33 , wherein the isolated and purified lipophilic anti-oxidant, wherein the lipophobic and the lipophilic antioxidants when provided to a patient provide an increase of over 13% as measured by ORAC(β-PE) from the patient's baseline antioxidant level.
48 . A dietary supplement comprising:
a nutritionally effective amount of two or more essential saccharides; an isolated and purified lipophobic oxygen-radical quencher; and an isolated and purified lipophilic oxygen-radical quencher, wherein the lipophobic and the lipophilic oxygen-radical quenchers combined have an oxygen-radical quencher value of greater than 6,000 μMol Trolox Equivalents (TE)/gram.
49 . The supplement of claim 48 , wherein the lipophobic and the lipophilic oxygen-radical quencher when provided to a patient provide an increase of over 13% as measured by ORAC(β-PE) from the patient's baseline antioxidant level.
50 . The supplement of claim 48 , wherein the lipophobic and the lipophilic oxygen-radical quencher are packaged for extended-release.
51 . The supplement of claim 48 , wherein the lipophobic oxygen-radical quencher is selected from the group consisting of one or more vitamin E selected from the group consisting of alpha, beta, delta, epsilon, gamma, zeta, eta, xi1, xi2, and sigma tocopherols, and alpha, beta, delta and gamma tocotrienols, analogs thereof, pharmaceutically acceptable salts thereof, and combinations thereof.
52 . The supplement of claim 48 , wherein the lipophilic oxygen-radical quencher is selected from the group consisting of flavonols, quercetin, kaempferol, myricetin, apigenin and derivates, analogs, pharmaceutically acceptable salts thereof, and combinations thereof.
53 . The supplement of claim 48 , further comprising two or more saccharides selected from the group consisting of galactose, glucose, mannose, N-acetylneuraminic acid, fucose, N-acetylgalactosamine, N-acetylglucosamine and xylose, derivates, analogs, pharmaceutically acceptable salts thereof, and combinations thereof.
54 . The supplement of claim 48 , further comprising six or more saccharides selected from the group consisting of galactose, glucose, mannose, N-acetylneuraminic acid, fucose, N-acetylgalactosamine, N-acetylglucosamine and xylose, derivates, analogs, pharmaceutically acceptable salts thereof, and combinations thereof.
55 . A system for measuring the antioxidant potential of a compositions comprising:
an oxygen sensor in fluid communication with a sample and an oxygen radical sensitive molecule in a solvent/water/surfactant mixture; wherein the oxygen radical sensitive sensor concurrently detects both lipophilic and lipophobic anti-oxidants in the solvent/water/surfactant mixture; a fluidic system in fluid communication with the oxygen sensor comprising one or more vessels, values and conduits; and a processor connected to the oxygen sensor and the fluidic system.
56 . The system of claim 55 , further comprising pH, ORP, conductivity or turbidity sensors in fluid communication with the fluidics system.Join the waitlist — get patent alerts
Track US2005087452A1 — get alerts on status changes and closely related new filings.
We store only your email — no account needed. See our privacy policy.